TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455862.2.1
(712 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO365044.1|CO365044 RTK1_23_G08.b1_A029 Roots minus pota... 75 2e-023
gb|DR389051.1|DR389051 RTHG1_32_D01.b1_A029 Roots plus adde... 75 2e-023
gb|DR742826.1|DR742826 RTCU1_7_F03.b2_A029 Roots plus added... 75 2e-023
gb|CF392189.1|CF392189 RTDR3_8_F01.b1_A022 Loblolly pine ro... 71 8e-022
gb|DR014514.1|DR014514 HEAT1_50_A06.b1_A029 Root at 37 C fo... 66 5e-021
gb|CR394075.1|CR394075 CR394075 RN Pinus pinaster cDNA clon... 62 6e-021
>gb|CO365044.1|CO365044 RTK1_23_G08.b1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_23_G08_A029 3', mRNA sequence
Length = 853
Score = 75.3 bits (158), Expect(2) = 2e-023
Identities = 25/58 (43%), Positives = 37/58 (63%)
Frame = +1 / +3
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACVPDGDGRPLSC 192
YP + M V +++W+G WATQ G+VK+DWS +PF SY ++ D C +G+G C
Sbjct: 402 YPNEQAMRVFSSIWNGDQWATQGGRVKIDWSYSPFVASYMNFNTDVCEWNGNGSTSQC 575
Score = 53.8 bits (111), Expect(2) = 2e-023
Identities = 20/46 (43%), Positives = 27/46 (58%)
Frame = +1 / +3
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSR 339
T+ W L+ A+ G + W R NY+ Y+YC D RF PAECS+
Sbjct: 582 TNWWNQNSLNYAQQGQLQWVRSNYLIYDYCKDYARFNYQLPAECSQ 719
>gb|DR389051.1|DR389051 RTHG1_32_D01.b1_A029 Roots plus added mercury Pinus taeda cDNA
clone RTHG1_32_D01_A029 3', mRNA sequence
Length = 782
Score = 75.3 bits (158), Expect(2) = 2e-023
Identities = 25/58 (43%), Positives = 37/58 (63%)
Frame = +1 / +3
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACVPDGDGRPLSC 192
YP + M V +++W+G WATQ G+VK+DWS +PF SY ++ D C +G+G C
Sbjct: 330 YPNEQAMRVFSSIWNGDQWATQGGRVKIDWSYSPFVASYMNFNTDVCEWNGNGSTSQC 503
Score = 53.8 bits (111), Expect(2) = 2e-023
Identities = 20/46 (43%), Positives = 27/46 (58%)
Frame = +1 / +3
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSR 339
T+ W L+ A+ G + W R NY+ Y+YC D RF PAECS+
Sbjct: 510 TNWWNQNSLNYAQQGQLQWVRSNYLIYDYCKDYARFNYQLPAECSQ 647
>gb|DR742826.1|DR742826 RTCU1_7_F03.b2_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_7_F03_A029 3', mRNA sequence
Length = 646
Score = 75.3 bits (158), Expect(2) = 2e-023
Identities = 25/58 (43%), Positives = 37/58 (63%)
Frame = +1 / +2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACVPDGDGRPLSC 192
YP + M V +++W+G WATQ G+VK+DWS +PF SY ++ D C +G+G C
Sbjct: 80 YPNEQAMRVFSSIWNGDQWATQGGRVKIDWSYSPFVASYMNFNTDVCEWNGNGSTSQC 253
Score = 53.8 bits (111), Expect(2) = 2e-023
Identities = 20/46 (43%), Positives = 27/46 (58%)
Frame = +1 / +2
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSR 339
T+ W L+ A+ G + W R NY+ Y+YC D RF PAECS+
Sbjct: 260 TNWWNQNSLNYAQQGQLQWVRSNYLIYDYCKDYARFNYQLPAECSQ 397
>gb|CF392189.1|CF392189 RTDR3_8_F01.b1_A022 Loblolly pine roots recovering from drought DR3
Pinus taeda cDNA clone RTDR3_8_F01_A022 3', mRNA
sequence
Length = 563
Score = 71.2 bits (149), Expect(2) = 8e-022
Identities = 23/52 (44%), Positives = 35/52 (67%)
Frame = +1 / +2
Query: 37 MSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACVPDGDGRPLSC 192
M V +++W+G WATQ G+VK+DWS++PF SY ++ D C +G+G C
Sbjct: 14 MRVFSSIWNGDQWATQGGRVKIDWSSSPFVASYMNFNTDVCEWNGNGSTSQC 169
Score = 52.4 bits (108), Expect(2) = 8e-022
Identities = 19/46 (41%), Positives = 27/46 (58%)
Frame = +1 / +2
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSR 339
T+ W L+ A+ G + W R NY+ Y+YC D RF PAEC++
Sbjct: 176 TNWWNQNSLNYAQQGQLQWVRSNYLIYDYCKDYARFNYQLPAECAQ 313
>gb|DR014514.1|DR014514 HEAT1_50_A06.b1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone
HEAT1_50_A06_A029 3', mRNA sequence
Length = 828
Score = 66.1 bits (138), Expect(2) = 5e-021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +1 / +2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADAC 159
YP +PM V+++LW+ WAT+ G K DW+ APF +YR + ++C
Sbjct: 311 YPNSQPMKVYSSLWNADDWATRGGLAKTDWTKAPFVATYRNFKVESC 451
Score = 57.0 bits (118), Expect(2) = 5e-021
Identities = 19/31 (61%), Positives = 22/31 (70%)
Frame = +1 / +2
Query: 256 WARQNYMHYNYCDDGWRFPQGFPAECSRN*F 348
W ++NYM YNYC D RFP GFP EC+R F
Sbjct: 584 WVQRNYMTYNYCTDSKRFPNGFPPECTRQNF 676
>gb|CR394075.1|CR394075 CR394075 RN Pinus pinaster cDNA clone RN73D05_1, mRNA sequence
Length = 556
Score = 62.0 bits (129), Expect(2) = 6e-021
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +1 / +3
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADA 156
YP + M V+++LW+ WAT+ G VK DWS APF S+R ++A A
Sbjct: 87 YPKSQAMRVYSSLWNADDWATRGGLVKTDWSKAPFVASFRNFNAPA 224
Score = 58.8 bits (122), Expect(2) = 6e-021
Identities = 23/52 (44%), Positives = 30/52 (57%)
Frame = +1 / +3
Query: 178 RPLSCPAGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAEC 333
R + PA ++ LD+ + + W R NYM Y+YC D RFPQG PAEC
Sbjct: 204 RNFNAPAASNFAAEEALDSNQEQKLQWVRNNYMIYDYCADTKRFPQGLPAEC 359
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 326,798,500
Number of Sequences: 355925
Number of extensions: 4701712
Number of successful extensions: 55716
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 9920846680
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)