TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2441276.2.1
(1796 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo li... 78 1e-046
gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo li... 78 1e-040
gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciat... 92 2e-032
gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo lib... 72 6e-021
gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo li... 72 6e-021
>gb|DR685262.1|DR685262 EST1075339 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWAB186 3' end, mRNA sequence
Length = 841
Score = 78.0 bits (164), Expect(5) = 1e-046
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = -2 / +2
Query: 1786 RGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRLS 1613
RG+NGELRLG+RRA + K+G L NQ ++L L A AVATKS+FH++YNPR S
Sbjct: 2 RGENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTS 175
Score = 75.8 bits (159), Expect(3) = 2e-022
Identities = 47/98 (47%), Positives = 53/98 (54%)
Frame = +3 / -2
Query: 1461 GSASPIPAMIPVHLLSEASSLSYLTLNLEPAENG*LKLLINFENGIMNSDWLNLGL**MW 1640
GSASPI ++PV LLS SS+S L L P +NG L+L F GIMNS L LGL*
Sbjct: 327 GSASPIAVIMPV*LLSAVSSVSNLILKRIPIDNGCLQLFTYF**GIMNSAGLVLGL*KT* 148
Query: 1641 NTLLVATACATLGSEPRFEHWL*RAGKADPFLS*AARR 1754
N L VATA A S R+ WL R P A R
Sbjct: 147 NMLFVATALAAAASTLRWAFWLERTEDRIPLFCLVALR 34
Score = 72.1 bits (151), Expect(4) = 5e-021
Identities = 40/107 (37%), Positives = 55/107 (51%)
Frame = -1 / +1
Query: 1787 EG**WGAKTWSAPCSSA*KWICFSSSL*PVLKSWFTT*CCTCCGHQKCVPHLLQPQIKPI 1608
EG* W K + KW SS *P S C CC H++ V LQ + K
Sbjct: 1 EG*KWRVKIRDSQSYQTEKWYSIFSSF*PECPSQCACCCS*CCCHKEHVSCFLQSKDKSS 180
Query: 1607 *IHYTIFEVYQELQSTIFCWFEVQSEI*ER*CF*KKMHRDHSRNW*C 1467
+HYT+ E+ ++LQ+TI W +Q+EI* R *+K+H + N *C
Sbjct: 181 RVHYTLSEICEKLQATIVYWNALQNEI*NRGYR*EKLHWHDNCNR*C 321
Score = 49.6 bits (102), Expect(5) = 1e-046
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = -2 / +2
Query: 1456 WRGSKWKCLMVRWDDDVDFRQPNRISPWEIELTSSVSGSHMSA 1328
W GSKW+ L V WD+ Q R+S WEIE + + +G ++S+
Sbjct: 335 WPGSKWRLLKVEWDEHAANEQQERVSAWEIEPSIAGAGLNVSS 463
Score = 49.2 bits (101), Expect(5) = 1e-046
Identities = 18/30 (60%), Positives = 24/30 (80%)
Frame = -2 / +2
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADP 1460
G RFK+R+E++D +ER TG+I IGDADP
Sbjct: 239 GMRFKMRFETEDTAERSYTGMITAIGDADP 328
Score = 47.3 bits (97), Expect(5) = 1e-046
Identities = 21/49 (42%), Positives = 28/49 (57%)
Frame = -2 / +2
Query: 1333 SAPNAKRLKPCLPHVNPDYLVPNGSGRPDFAESAQFHKVLQGQELLGYR 1187
S KRL+ L D +P+GS DF ES +F KVLQGQE + ++
Sbjct: 461 SGTRTKRLRTTLLSTPVDVAIPDGSRLSDFEESIRFQKVLQGQENMSFK 607
Score = 38.2 bits (77), Expect(4) = 5e-021
Identities = 17/28 (60%), Positives = 21/28 (75%)
Frame = -1 / +1
Query: 1052 TRKP*VFLPLLRLWGVSKIPKGLARSRS 969
TRK F ++RLW + KIPKGLARSR+
Sbjct: 727 TRKIRHFK*VIRLWRIYKIPKGLARSRN 810
Score = 37.7 bits (76), Expect(5) = 1e-046
Identities = 19/53 (35%), Positives = 26/53 (49%)
Frame = -2 / +2
Query: 1126 IDERSCSNDASNIIPGVPRIGVRTPLGSPRFSYRCSGFGESPRFQKVLQGQEV 968
++ + C+ A + + L S R FGES RFQKVLQGQE+
Sbjct: 653 LERKGCNAVAERFETARTENAIWSSLERSDISSRLLDFGESIRFQKVLQGQEI 811
Score = 37.7 bits (76), Expect(3) = 2e-022
Identities = 15/23 (65%), Positives = 16/23 (69%)
Frame = +3 / -2
Query: 1203 S*PCKTLWNWADSAKSGRPLPFG 1271
S*PCKT WN DS+KS LP G
Sbjct: 591 S*PCKTFWNLMDSSKSDNLLPSG 523
Score = 37.7 bits (76), Expect(3) = 2e-022
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = +3 / -2
Query: 972 S*PCKTFWNLGDSPK 1016
S*PCKTFWNL DSPK
Sbjct: 807 S*PCKTFWNLIDSPK 763
Score = 32.7 bits (65), Expect(4) = 5e-021
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = -1 / +1
Query: 1457 VAWFEMEMFDGPMG*RCRFSSTKQDFSLGD* 1365
+AWF+ME+ +G MG* C + + F +G+*
Sbjct: 334 MAWFKMEVIEG*MG*TCSK*TAGKSFCMGN* 426
Score = 22.1 bits (42), Expect(4) = 5e-021
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -3 / +3
Query: 1245 LRNLPNSTRSCKVKNYWVIE 1186
L+N +S RSCKVK +++
Sbjct: 549 LKNP*DSKRSCKVKKICLLK 608
>gb|DT632793.1|DT632793 EST1147724 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFN79 3' end, mRNA sequence
Length = 783
Score = 78.0 bits (164), Expect(4) = 1e-040
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = -2 / +2
Query: 1786 RGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRLS 1613
RG+NGELRLG+RRA + K+G L NQ ++L L A AVATKS+FH++YNPR S
Sbjct: 2 RGENGELRLGIRRATRQKSGILSSVLSNQNAHLSVLAAAASAVATKSMFHVFYNPRTS 175
Score = 49.6 bits (102), Expect(4) = 1e-040
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = -2 / +2
Query: 1456 WRGSKWKCLMVRWDDDVDFRQPNRISPWEIELTSSVSGSHMSA 1328
W GSKW+ L V WD+ Q R+S WEIE + + +G ++S+
Sbjct: 335 WPGSKWRLLKVEWDEHAANEQQERVSAWEIEPSIAGAGLNVSS 463
Score = 49.2 bits (101), Expect(4) = 1e-040
Identities = 18/30 (60%), Positives = 24/30 (80%)
Frame = -2 / +2
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADP 1460
G RFK+R+E++D +ER TG+I IGDADP
Sbjct: 239 GMRFKMRFETEDTAERSYTGMITAIGDADP 328
Score = 47.3 bits (97), Expect(4) = 1e-040
Identities = 21/49 (42%), Positives = 28/49 (57%)
Frame = -2 / +2
Query: 1333 SAPNAKRLKPCLPHVNPDYLVPNGSGRPDFAESAQFHKVLQGQELLGYR 1187
S KRL+ L D +P+GS DF ES +F KVLQGQE + ++
Sbjct: 461 SGTRTKRLRTTLLSTPVDVAIPDGSRLSDFEESIRFQKVLQGQENMSFK 607
>gb|BX250119.1|BX250119 BX250119 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP032F08, mRNA sequence
Length = 674
Score = 91.8 bits (194), Expect(2) = 2e-021
Identities = 48/83 (57%), Positives = 53/83 (63%)
Frame = +3 / -1
Query: 1458 MGSASPIPAMIPVHLLSEASSLSYLTLNLEPAENG*LKLLINFENGIMNSDWLNLGL**M 1637
+GS SPIP ++PV+LLS ASS+S L LN P EN LKL GI NS L LGL**M
Sbjct: 575 IGSTSPIPTIVPVYLLSVASSVSNLILNRIPIENEWLKLFTYL*YGITNSVGLVLGL**M 396
Query: 1638 WNTLLVATACATLGSEPRFEHWL 1706
WN L VATA AT S P WL
Sbjct: 395 WNILFVATA*ATAASTPECMPWL 327
Score = 81.3 bits (171), Expect(3) = 2e-032
Identities = 36/61 (59%), Positives = 44/61 (72%)
Frame = -2 / +1
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
LFLR +NGELRLG+RRA+Q ++ L + + G L VAHAVATKS+FHIYYNPR
Sbjct: 238 LFLRDENGELRLGIRRASQQQSSVPSSVLSSHGIHSGVLAAVAHAVATKSMFHIYYNPRT 417
Query: 1615 S 1613
S
Sbjct: 418 S 420
Score = 56.1 bits (116), Expect(3) = 2e-032
Identities = 21/31 (67%), Positives = 26/31 (83%)
Frame = -2 / +1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPM 1457
G RFK+R+E++DA+ERR TG I GIGD DPM
Sbjct: 484 GMRFKMRFETEDATERRYTGTIVGIGDVDPM 576
Score = 41.8 bits (85), Expect(3) = 2e-032
Identities = 13/31 (41%), Positives = 19/31 (61%)
Frame = -2 / +1
Query: 1456 WRGSKWKCLMVRWDDDVDFRQPNRISPWEIE 1364
W S+W+ V WD+ + +R+SPWEIE
Sbjct: 580 WPNSRWRSFKVGWDEHAAQXRQDRVSPWEIE 672
Score = 32.2 bits (64), Expect(2) = 2e-021
Identities = 15/29 (51%), Positives = 16/29 (55%)
Frame = +1 / -2
Query: 1363 AQSPKEKSCLVDENLHRHPIGPSNISISN 1449
A+ P EK C LH HPI P ISI N
Sbjct: 673 ARFPMEKPCPAXPVLHAHPILP*TISILN 587
>gb|DN612931.1|DN612931 EST965981 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PSABL95, mRNA sequence
Length = 596
Score = 71.6 bits (150), Expect(3) = 6e-021
Identities = 28/61 (45%), Positives = 41/61 (67%)
Frame = -2 / -1
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
+FLRG+NGELR+GVRRA + +N + + +LG + +HAV TK++F +YY PR
Sbjct: 374 IFLRGENGELRVGVRRAMRQQNNVPSSVISSHSMHLGVIATASHAVTTKTMFSVYYKPRT 195
Query: 1615 S 1613
S
Sbjct: 194 S 192
Score = 45.1 bits (92), Expect(3) = 6e-021
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = -2 / -1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPM 1457
G RFK+++E ++ E+R TG I GI DADP+
Sbjct: 128 GMRFKMKFEGEEVPEQRFTGTIVGISDADPV 36
Score = 23.1 bits (44), Expect(3) = 6e-021
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -2 / -1
Query: 1456 WRGSKWKCL 1430
W SKW+CL
Sbjct: 32 WPNSKWRCL 6
>gb|DT628876.1|DT628876 EST1155446 Subtracted pine embryo library, Lib_B Pinus taeda cDNA
clone PIMD303 5' end, mRNA sequence
Length = 596
Score = 71.6 bits (150), Expect(3) = 6e-021
Identities = 28/61 (45%), Positives = 41/61 (67%)
Frame = -2 / +1
Query: 1795 LFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCSNLGSLPNVAHAVATKSVFHIYYNPRL 1616
+FLRG+NGELR+GVRRA + +N + + +LG + +HAV TK++F +YY PR
Sbjct: 223 IFLRGENGELRVGVRRAMRQQNNVPSSVISSHSMHLGVIATASHAVTTKTMFSVYYKPRT 402
Query: 1615 S 1613
S
Sbjct: 403 S 405
Score = 45.1 bits (92), Expect(3) = 6e-021
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = -2 / +1
Query: 1549 GSRFKVRYESDDASERRCTGIIAGIGDADPM 1457
G RFK+++E ++ E+R TG I GI DADP+
Sbjct: 469 GMRFKMKFEGEEVPEQRFTGTIVGISDADPV 561
Score = 23.1 bits (44), Expect(3) = 6e-021
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -2 / +1
Query: 1456 WRGSKWKCL 1430
W SKW+CL
Sbjct: 565 WPNSKWRCL 591
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,081,124,214
Number of Sequences: 355925
Number of extensions: 17580717
Number of successful extensions: 77533
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 55
effective length of database: 52,849,870
effective search space used: 28697479410
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)