TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430991.2.1
(515 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT637161.1|DT637161 EST1152092 Normalized pine embryo li... 191 2e-056
gb|DR692110.1|DR692110 EST1082197 Normalized pine embryo li... 191 2e-056
gb|CX713339.1|CX713339 RTPQ1_8_B07.g1_A032 Roots treated wi... 132 1e-033
gb|DT633602.1|DT633602 EST1148533 Normalized pine embryo li... 131 4e-033
gb|DR686821.1|DR686821 EST1076899 Normalized pine embryo li... 131 4e-033
gb|DR053011.1|DR053011 RTCA1_8_A01.g1_A029 Roots minus calc... 132 2e-030
gb|DR169155.1|DR169155 RTPHOS1_30_A06.g1_A029 Roots minus p... 121 3e-030
gb|BQ655460.1|BQ655460 NXRV094_H04_F NXRV (Nsf Xylem Root w... 108 2e-026
gb|BQ107024.1|BQ107024 NXLV085_F11_F NXLV (Nsf Xylem Late w... 108 3e-023
gb|DR686620.1|DR686620 EST1076698 Normalized pine embryo li... 108 3e-023
gb|DT633664.1|DT633664 EST1148595 Normalized pine embryo li... 108 3e-023
gb|BX254765.1|BX254765 BX254765 Pinus pinaster differenciat... 97 3e-022
gb|DR744317.1|DR744317 RTCU1_21_E03.g1_A029 Roots plus adde... 103 8e-022
gb|BQ699412.1|BQ699412 NXRV127_E03_F NXRV (Nsf Xylem Root w... 83 1e-021
>gb|DT637161.1|DT637161 EST1152092 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMH039 3' end, mRNA sequence
Length = 879
Score = 191 bits (411), Expect(2) = 2e-056
Identities = 72/112 (64%), Positives = 89/112 (79%)
Frame = -2 / +1
Query: 337 LRDNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYR 158
L DN LYHFCVFSDN+LA SVVVNST N+ +P+++VFH+VTDE+N+ +AWF MNDYR
Sbjct: 211 LTDNKLYHFCVFSDNVLATSVVVNSTVQNADYPEQLVFHIVTDEINHGATQAWFAMNDYR 390
Query: 157 GVTVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGTPIKFRNPK 2
G +E+Q V+DF WLNASYVPVLKQL++ TQ +YF GS + IKFRNPK
Sbjct: 391 GAAIEVQNVDDFNWLNASYVPVLKQLRDEETQSYYFKGSSDMKSAIKFRNPK 546
Score = 103 bits (220), Expect(2) = 5e-022
Identities = 56/105 (53%), Positives = 64/105 (60%)
Frame = +3 / -2
Query: 3 FGFLNLIGVPRLPLPEK*NFCVAAFCSCLRTGTYDALSQVKSSTFCISTVTPL*SFMPNH 182
FG NLI + P K* CV++ SC TGT DA SQ+KSSTFC S PL*SF+ N
Sbjct: 545 FGLRNLIALFISEEPLK**LCVSSSRSCFNTGT*DAFSQLKSSTFCTSMAAPL*SFIANQ 366
Query: 183 AFIGA*FNSSVTK*KTTLSGCLEFIAVELTTTETARMLSEKTQK* 317
A + * SSVT KT+ SG F VE TTT+ A LSE TQK*
Sbjct: 365 ACVAP*LISSVTIWKTSCSG*SAF*TVEFTTTDVASTLSENTQK* 231
Score = 47.8 bits (98), Expect(2) = 2e-056
Identities = 17/53 (32%), Positives = 30/53 (56%)
Frame = -2 / +1
Query: 514 SLXEKSKVXXXKXTXXXXIAAEELPKGLYCLGVRLTMXWFKSPELQRKFSDRS 356
+L +++ + T +AAE +PKGLYC G L+ W K+ +LQ+ ++
Sbjct: 40 ALEDRTNAATIQSTTFGQLAAEAVPKGLYCFGTMLSKEWSKNHKLQKHMDQQN 198
Score = 21.7 bits (41), Expect(2) = 5e-022
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +3 / -2
Query: 420 PRQ*SPFGSSSAA 458
P+Q* P G++SAA
Sbjct: 134 PKQ*RPLGTASAA 96
>gb|DR692110.1|DR692110 EST1082197 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWADI14 3' end, mRNA sequence
Length = 741
Score = 191 bits (411), Expect(2) = 2e-056
Identities = 72/112 (64%), Positives = 89/112 (79%)
Frame = -2 / +2
Query: 337 LRDNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYR 158
L DN LYHFCVFSDN+LA SVVVNST N+ +P+++VFH+VTDE+N+ +AWF MNDYR
Sbjct: 203 LTDNKLYHFCVFSDNVLATSVVVNSTVQNADYPEQLVFHIVTDEINHGATQAWFAMNDYR 382
Query: 157 GVTVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGTPIKFRNPK 2
G +E+Q V+DF WLNASYVPVLKQL++ TQ +YF GS + IKFRNPK
Sbjct: 383 GAAIEVQNVDDFNWLNASYVPVLKQLRDEETQSYYFKGSSDMKSAIKFRNPK 538
Score = 103 bits (220), Expect(2) = 4e-022
Identities = 56/105 (53%), Positives = 64/105 (60%)
Frame = +3 / -1
Query: 3 FGFLNLIGVPRLPLPEK*NFCVAAFCSCLRTGTYDALSQVKSSTFCISTVTPL*SFMPNH 182
FG NLI + P K* CV++ SC TGT DA SQ+KSSTFC S PL*SF+ N
Sbjct: 537 FGLRNLIALFISEEPLK**LCVSSSRSCFNTGT*DAFSQLKSSTFCTSMAAPL*SFIANQ 358
Query: 183 AFIGA*FNSSVTK*KTTLSGCLEFIAVELTTTETARMLSEKTQK* 317
A + * SSVT KT+ SG F VE TTT+ A LSE TQK*
Sbjct: 357 ACVAP*LISSVTIWKTSCSG*SAF*TVEFTTTDVASTLSENTQK* 223
Score = 47.8 bits (98), Expect(2) = 2e-056
Identities = 17/53 (32%), Positives = 30/53 (56%)
Frame = -2 / +2
Query: 514 SLXEKSKVXXXKXTXXXXIAAEELPKGLYCLGVRLTMXWFKSPELQRKFSDRS 356
+L +++ + T +AAE +PKGLYC G L+ W K+ +LQ+ ++
Sbjct: 32 ALEDRTNAATIQSTTFGQLAAEAVPKGLYCFGTMLSKEWSKNHKLQKHMDQQN 190
Score = 21.7 bits (41), Expect(2) = 4e-022
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = +3 / -1
Query: 420 PRQ*SPFGSSSAA 458
P+Q* P G++SAA
Sbjct: 126 PKQ*RPLGTASAA 88
>gb|CX713339.1|CX713339 RTPQ1_8_B07.g1_A032 Roots treated with paraquat Pinus taeda cDNA
clone RTPQ1_8_B07_A032 5', mRNA sequence
Length = 657
Score = 132 bits (283), Expect(2) = 1e-033
Identities = 52/102 (50%), Positives = 72/102 (70%)
Frame = -2 / +2
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 248 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 427
Query: 151 TVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGT 26
VE++ VED+ +LN+SYVPVL+QL++A Q+FYF T
Sbjct: 428 HVEVKAVEDYKFLNSSYVPVLRQLESANLQRFYFENKLENAT 553
Score = 29.9 bits (59), Expect(2) = 1e-033
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +2
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 131 IAAKSIPKSIHCLSMRLMEERISQPE 208
>gb|DT633602.1|DT633602 EST1148533 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFW66 3' end, mRNA sequence
Length = 879
Score = 131 bits (280), Expect(2) = 4e-033
Identities = 52/102 (50%), Positives = 71/102 (69%)
Frame = -2 / +3
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 195 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 374
Query: 151 TVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGT 26
VE++ VED+ +LN+SYVPVL+QL+ A Q+FYF T
Sbjct: 375 HVEVKAVEDYKFLNSSYVPVLRQLEPANLQRFYFENKLENAT 500
Score = 29.9 bits (59), Expect(2) = 4e-033
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +3
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 78 IAAKSIPKSIHCLSMRLMEERISQPE 155
>gb|DR686821.1|DR686821 EST1076899 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABK22 3' end, mRNA sequence
Length = 742
Score = 131 bits (280), Expect(2) = 4e-033
Identities = 52/102 (50%), Positives = 71/102 (69%)
Frame = -2 / +1
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 187 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 366
Query: 151 TVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGT 26
VE++ VED+ +LN+SYVPVL+QL+ A Q+FYF T
Sbjct: 367 HVEVKAVEDYKFLNSSYVPVLRQLEPANLQRFYFENKLENAT 492
Score = 29.9 bits (59), Expect(2) = 4e-033
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +1
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 70 IAAKSIPKSIHCLSMRLMEERISQPE 147
>gb|DR053011.1|DR053011 RTCA1_8_A01.g1_A029 Roots minus calcium Pinus taeda cDNA clone
RTCA1_8_A01_A029 5', mRNA sequence
Length = 802
Score = 132 bits (283), Expect = 2e-030
Identities = 52/102 (50%), Positives = 72/102 (70%)
Frame = -2 / +3
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 66 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 245
Query: 151 TVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGT 26
VE++ VED+ +LN+SYVPVL+QL++A Q+FYF T
Sbjct: 246 HVEVKAVEDYKFLNSSYVPVLRQLESANLQRFYFENKLENAT 371
>gb|DR169155.1|DR169155 RTPHOS1_30_A06.g1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_30_A06_A029 5', mRNA sequence
Length = 568
Score = 121 bits (259), Expect(2) = 3e-030
Identities = 47/87 (54%), Positives = 66/87 (75%)
Frame = -2 / +2
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 302 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 481
Query: 151 TVEIQKVEDFTWLNASYVPVLKQLQNA 71
VE++ VED+ +LN+SYVPVL+QL++A
Sbjct: 482 HVEVKAVEDYKFLNSSYVPVLRQLESA 562
Score = 29.9 bits (59), Expect(2) = 3e-030
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +2
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 185 IAAKSIPKSIHCLSMRLMEERISQPE 262
>gb|BQ655460.1|BQ655460 NXRV094_H04_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV094_H04 5' similar to Arabidopsis thaliana
sequence At3g25140 glycosyl transferase, putative see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 492
Score = 108 bits (231), Expect(2) = 2e-026
Identities = 41/83 (49%), Positives = 59/83 (71%)
Frame = -2 / +1
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGV 152
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 226 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNGS 405
Query: 151 TVEIQKVEDFTWLNASYVPVLKQ 83
VE++ ED+ + N+SYVP ++Q
Sbjct: 406 HVEVKAXEDYKFXNSSYVPXMRQ 474
Score = 29.9 bits (59), Expect(2) = 2e-026
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +1
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 109 IAAKSIPKSIHCLSMRLMEERISQPE 186
>gb|BQ107024.1|BQ107024 NXLV085_F11_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
clone NXLV085_F11 5' similar to Arabidopsis thaliana
sequence At2g20807 unknown protein see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 732
Score = 108 bits (230), Expect = 3e-023
Identities = 40/62 (64%), Positives = 48/62 (77%)
Frame = -2 / +1
Query: 187 KAWFGMNDYRGVTVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGTPIKFRN 8
+AWF MNDYRG T+E+Q V+DF WLNASYVPVLKQL++ TQ +YF G + IKFRN
Sbjct: 22 QAWFAMNDYRGATIEVQNVDDFNWLNASYVPVLKQLRDEETQSYYFKGFSDMKSAIKFRN 201
Query: 7 PK 2
PK
Sbjct: 202 PK 207
>gb|DR686620.1|DR686620 EST1076698 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABI03 3' end, mRNA sequence
Length = 866
Score = 108 bits (230), Expect = 3e-023
Identities = 41/79 (51%), Positives = 59/79 (74%)
Frame = -2 / +2
Query: 286 AVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGVTVEIQKVEDFTWLNA 107
A SVVVNST +N++ +K VFHLVTD+LN+ MK WF +N + ++ V+DF WLN+
Sbjct: 2 AASVVVNSTIMNAKESEKHVFHLVTDKLNFGAMKMWFLLNPPGKAAIHVENVDDFKWLNS 181
Query: 106 SYVPVLKQLQNAATQKFYF 50
SY PVL+QL++AA +++YF
Sbjct: 182 SYCPVLRQLESAAMKEYYF 238
>gb|DT633664.1|DT633664 EST1148595 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMFX37 3' end, mRNA sequence
Length = 812
Score = 108 bits (230), Expect = 3e-023
Identities = 41/79 (51%), Positives = 59/79 (74%)
Frame = -2 / +1
Query: 286 AVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRGVTVEIQKVEDFTWLNA 107
A SVVVNST +N++ +K VFHLVTD+LN+ MK WF +N + ++ V+DF WLN+
Sbjct: 10 AASVVVNSTIMNAKESEKHVFHLVTDKLNFGAMKMWFLLNPPGKAAIHVENVDDFKWLNS 189
Query: 106 SYVPVLKQLQNAATQKFYF 50
SY PVL+QL++AA +++YF
Sbjct: 190 SYCPVLRQLESAAMKEYYF 246
>gb|BX254765.1|BX254765 BX254765 Pinus pinaster differenciating xylem adult Pinus pinaster
cDNA clone PP104A12, mRNA sequence
Length = 629
Score = 96.8 bits (205), Expect(2) = 3e-022
Identities = 38/81 (46%), Positives = 52/81 (64%)
Frame = -2 / +2
Query: 358 SPAVQSNLRDNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAW 179
+P + L DNS HF + +DN+LA SVVV S NS PDKVVFH++TD+ YAPM AW
Sbjct: 386 APELVPCLTDNSYQHFVLATDNVLAASVVVLSAVRNSAKPDKVVFHVITDKKTYAPMHAW 565
Query: 178 FGMNDYRGVTVEIQKVEDFTW 116
F ++ +E++ + FTW
Sbjct: 566 FALHPIPSAIIEVKGLHQFTW 628
Score = 27.6 bits (54), Expect(2) = 3e-022
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -2 / +2
Query: 511 LXEKSKVXXXKXTXXXXIAAEELPKGLYCLGVRL 410
L +K++ IA+ +PKG++CL ++L
Sbjct: 239 LEQKARSSRMHEYLYRHIASSSIPKGMHCLSLKL 340
>gb|DR744317.1|DR744317 RTCU1_21_E03.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_21_E03_A029 5', mRNA sequence
Length = 749
Score = 103 bits (220), Expect = 8e-022
Identities = 43/111 (38%), Positives = 64/111 (57%)
Frame = -2 / +3
Query: 358 SPAVQSNLRDNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAW 179
+P L DNS +HF + +DN+LA SVVV+S A N P+ VFH++TD+ YA M AW
Sbjct: 60 NPEFVPRLTDNSYHHFVLPTDNVLAASVVVSSAAKNFATPETAVFHVITDKKTYAAMHAW 239
Query: 178 FGMNDYRGVTVEIQKVEDFTWLNASYVPVLKQLQNAATQKFYFSGSGSRGT 26
F ++ +E++ V F WL VPVL+ ++ + + Y+ G GT
Sbjct: 240 FALHPLAPAIIEVKSVHHFEWLTKENVPVLEVMETQSEIRRYYHGGHIIGT 392
>gb|BQ699412.1|BQ699412 NXRV127_E03_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV127_E03 5' similar to Arabidopsis thaliana
sequence At3g25140 glycosyl transferase, putative see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 544
Score = 82.6 bits (174), Expect(3) = 1e-021
Identities = 31/59 (52%), Positives = 42/59 (71%)
Frame = -2 / +1
Query: 331 DNSLYHFCVFSDNILAVSVVVNSTAINSRHPDKVVFHLVTDELNYAPMKAWFGMNDYRG 155
D +LYH+ +FSDN++A SVVVNS N++ P K VFH+VTD++N A M+ WF M G
Sbjct: 217 DPNLYHYAIFSDNVIAASVVVNSAVKNAKEPWKHVFHVVTDKMNLAAMQVWFKMRSLNG 393
Score = 29.9 bits (59), Expect(3) = 1e-021
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -2 / +1
Query: 460 IAAEELPKGLYCLGVRLTMXWFKSPE 383
IAA+ +PK ++CL +RL PE
Sbjct: 100 IAAKSIPKSIHCLSMRLMEERISQPE 177
Score = 27.2 bits (53), Expect(3) = 1e-021
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -2 / +3
Query: 97 PVLKQLQNAATQKFYF 50
PVL+QL++A Q FYF
Sbjct: 453 PVLRQLESANLQXFYF 500
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 259,410,169
Number of Sequences: 355925
Number of extensions: 3436391
Number of successful extensions: 45170
Number of sequences better than 1.0e-020: 17
length of database: 72,425,745
effective HSP length: 51
effective length of database: 54,273,570
effective search space used: 6512828400
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)