TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405087.2.1
(737 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DT634393.1|DT634393 EST1149324 Normalized pine embryo li... 101 5e-038
gb|DR687633.1|DR687633 EST1077715 Normalized pine embryo li... 101 5e-038
gb|DR168933.1|DR168933 RTPHOS1_28_G05.g1_A029 Roots minus p... 81 7e-032
gb|DR057730.1|DR057730 RTNIT1_7_B04.g1_A029 Roots minus nit... 81 1e-025
gb|DR057381.1|DR057381 RTNIT1_5_B04.g1_A029 Roots minus nit... 81 3e-025
gb|CO366556.1|CO366556 RTK1_28_C09.g1_A029 Roots minus pota... 81 2e-024
>gb|DT634393.1|DT634393 EST1149324 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PIMG549 3' end, mRNA sequence
Length = 827
Score = 101 bits (216), Expect(2) = 5e-038
Identities = 44/96 (45%), Positives = 60/96 (62%)
Frame = +2 / +1
Query: 254 GFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYYPSHSRETSNNSKITFALDQN 433
G VLST+LT+ EI +L SG +LP +FDA IC+SGS +YYP+ + S D +
Sbjct: 25 GLVLSTALTVDEILGMLNSGNILPHEFDALICSSGSELYYPTIPAYPDDGSNKKLCPDPD 204
Query: 434 HQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQI 541
+ SHI+YRWGGEGLRK + S + + + ER I
Sbjct: 205 YDSHIDYRWGGEGLRKTMSLLTASERDGQEKQERVI 312
Score = 76.2 bits (160), Expect(2) = 5e-038
Identities = 29/69 (42%), Positives = 51/69 (73%)
Frame = +2 / +1
Query: 530 ERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPI 709
+ ++IFED +HS+A+CLA+ V + + + E R+ +R++ LRC+ ++ ++T+L V+P+
Sbjct: 298 QERVIFEDAKHSNAHCLAYVVKDSTRVRKVDEFRQRLRMRGLRCHPMFCRNSTQLHVIPL 477
Query: 710 HASRSQALR 736
ASRSQALR
Sbjct: 478 LASRSQALR 504
>gb|DR687633.1|DR687633 EST1077715 Normalized pine embryo library, Lib_D Pinus taeda cDNA
clone PWABU74 3' end, mRNA sequence
Length = 797
Score = 101 bits (216), Expect(2) = 5e-038
Identities = 44/96 (45%), Positives = 60/96 (62%)
Frame = +2 / +2
Query: 254 GFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYYPSHSRETSNNSKITFALDQN 433
G VLST+LT+ EI +L SG +LP +FDA IC+SGS +YYP+ + S D +
Sbjct: 17 GLVLSTALTVDEILGMLNSGNILPHEFDALICSSGSELYYPTIPAYPDDGSNKKLCPDPD 196
Query: 434 HQSHIEYRWGGEGLRKYLVKWATSVVERKGRTERQI 541
+ SHI+YRWGGEGLRK + S + + + ER I
Sbjct: 197 YDSHIDYRWGGEGLRKTMSLLTASERDGQEKQERVI 304
Score = 76.2 bits (160), Expect(2) = 5e-038
Identities = 29/69 (42%), Positives = 51/69 (73%)
Frame = +2 / +2
Query: 530 ERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHSATRLSVVPI 709
+ ++IFED +HS+A+CLA+ V + + + E R+ +R++ LRC+ ++ ++T+L V+P+
Sbjct: 290 QERVIFEDAKHSNAHCLAYVVKDSTRVRKVDEFRQRLRMRGLRCHPMFCRNSTQLHVIPL 469
Query: 710 HASRSQALR 736
ASRSQALR
Sbjct: 470 LASRSQALR 496
>gb|DR168933.1|DR168933 RTPHOS1_28_G05.g1_A029 Roots minus phosphorous Pinus taeda cDNA
clone RTPHOS1_28_G05_A029 5', mRNA sequence
Length = 537
Score = 81.3 bits (171), Expect(3) = 7e-032
Identities = 33/78 (42%), Positives = 53/78 (67%)
Frame = +2 / +1
Query: 503 SVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHS 682
S+ + +G + + ED E S+ +CLA++V N + P ELR+ +R++ LRC+ +Y +
Sbjct: 292 SMKKLRGPENSRFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQN 471
Query: 683 ATRLSVVPIHASRSQALR 736
+RL V+P+HASRSQALR
Sbjct: 472 CSRLRVLPLHASRSQALR 525
Score = 53.3 bits (110), Expect(3) = 7e-032
Identities = 24/58 (41%), Positives = 35/58 (60%)
Frame = +2 / +1
Query: 200 IIRNAIEVIHKENMSGSTGFVLSTSLTISEIHSLLLSGGMLPTDFDAFICNSGSNIYY 373
II+ + + S GF+LST+LT+ E S+L S G+ + FDA IC+SGS + Y
Sbjct: 25 IIQEIMNAVCSAGNFSSPGFILSTALTVPETISMLQSIGIKVSAFDALICSSGSQLLY 198
Score = 40.9 bits (83), Expect(3) = 7e-032
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2 / +1
Query: 425 DQNHQSHIEYRWGGEGLRKYLVK 493
D ++ SHI+YRWG EGLRK + K
Sbjct: 235 DPDYDSHIDYRWGAEGLRKSMKK 303
>gb|DR057730.1|DR057730 RTNIT1_7_B04.g1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_7_B04_A029 5', mRNA sequence
Length = 929
Score = 81.3 bits (171), Expect(3) = 1e-025
Identities = 33/78 (42%), Positives = 53/78 (67%)
Frame = +2 / +3
Query: 503 SVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHS 682
S+ + +G + + ED E S+ +CLA++V N + P ELR+ +R++ LRC+ +Y +
Sbjct: 186 SMKKLRGPENSRFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQN 365
Query: 683 ATRLSVVPIHASRSQALR 736
+RL V+P+HASRSQALR
Sbjct: 366 CSRLRVLPLHASRSQALR 419
Score = 40.9 bits (83), Expect(3) = 1e-025
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2 / +3
Query: 425 DQNHQSHIEYRWGGEGLRKYLVK 493
D ++ SHI+YRWG EGLRK + K
Sbjct: 129 DPDYDSHIDYRWGAEGLRKSMKK 197
Score = 31.8 bits (63), Expect(3) = 1e-025
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = +2 / +3
Query: 287 EIHSLLLSGGMLPTDFDAFICNSGSNIYY 373
E S+L S G+ + FDA IC+SGS + Y
Sbjct: 6 ETISMLQSIGIKVSAFDALICSSGSQLLY 92
>gb|DR057381.1|DR057381 RTNIT1_5_B04.g1_A029 Roots minus nitrogen Pinus taeda cDNA clone
RTNIT1_5_B04_A029 5', mRNA sequence
Length = 829
Score = 81.3 bits (171), Expect(3) = 3e-025
Identities = 33/78 (42%), Positives = 53/78 (67%)
Frame = +2 / +3
Query: 503 SVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHS 682
S+ + +G + + ED E S+ +CLA++V N + P ELR+ +R++ LRC+ +Y +
Sbjct: 192 SMKKLRGPENSRFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQN 371
Query: 683 ATRLSVVPIHASRSQALR 736
+RL V+P+HASRSQALR
Sbjct: 372 CSRLRVLPLHASRSQALR 425
Score = 40.9 bits (83), Expect(3) = 3e-025
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2 / +3
Query: 425 DQNHQSHIEYRWGGEGLRKYLVK 493
D ++ SHI+YRWG EGLRK + K
Sbjct: 135 DPDYDSHIDYRWGAEGLRKSMKK 203
Score = 30.9 bits (61), Expect(3) = 3e-025
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +2 / +3
Query: 278 TISEIHSLLLSGGMLPTDFDAFICNSGSNIYY 373
T+ E S+L S + + FDA IC+SGS + Y
Sbjct: 3 TVPETISMLQSIFIKVSAFDALICSSGSQLLY 98
>gb|CO366556.1|CO366556 RTK1_28_C09.g1_A029 Roots minus potassium Pinus taeda cDNA clone
RTK1_28_C09_A029 5', mRNA sequence
Length = 851
Score = 81.3 bits (171), Expect(3) = 2e-024
Identities = 33/78 (42%), Positives = 53/78 (67%)
Frame = +2 / -2
Query: 503 SVVERKGRTERQIIFEDPEHSSAYCLAFRVVNPNHLPPLKELRKLMRIQSLRCNALYNHS 682
S+ + +G + + ED E S+ +CLA++V N + P ELR+ +R++ LRC+ +Y +
Sbjct: 697 SMKKLRGPENSRFLAEDAEASNFHCLAYQVKNISTAPKADELRRQLRMRGLRCHVIYTQN 518
Query: 683 ATRLSVVPIHASRSQALR 736
+RL V+P+HASRSQALR
Sbjct: 517 CSRLRVLPLHASRSQALR 464
Score = 40.9 bits (83), Expect(3) = 2e-024
Identities = 14/23 (60%), Positives = 18/23 (78%)
Frame = +2 / -2
Query: 425 DQNHQSHIEYRWGGEGLRKYLVK 493
D ++ SHI+YRWG EGLRK + K
Sbjct: 754 DPDYDSHIDYRWGAEGLRKSMKK 686
Score = 27.6 bits (54), Expect(3) = 2e-024
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +2 / -2
Query: 314 GMLPTDFDAFICNSGSNIYY 373
G+ + FDA IC+SGS + Y
Sbjct: 850 GIKVSAFDALICSSGSQLLY 791
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 392,977,367
Number of Sequences: 355925
Number of extensions: 5511334
Number of successful extensions: 40645
Number of sequences better than 1.0e-020: 6
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10406105485
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)