TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192420.2.1
(738 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CF665105.1|CF665105 RTCNT1_14_A11.b1_A029 Root control P... 299 2e-080
gb|DR743729.1|DR743729 RTCU1_17_G02.g1_A029 Roots plus adde... 297 4e-080
gb|CF397297.1|CF397297 RTDS3_2_D11.g1_A022 Drought-stressed... 177 1e-043
gb|CF397205.1|CF397205 RTDS3_2_D11.b2_A022 Drought-stressed... 144 9e-034
gb|CF400358.1|CF400358 RTWW1_4_D05.g1_A015 Well-watered lob... 67 9e-025
>gb|CF665105.1|CF665105 RTCNT1_14_A11.b1_A029 Root control Pinus taeda cDNA clone
RTCNT1_14_A11_A029 3', mRNA sequence
Length = 773
Score = 299 bits (647), Expect = 2e-080
Identities = 126/144 (87%), Positives = 135/144 (93%)
Frame = +1 / +1
Query: 7 QKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININLRTVQIAADVNIDEV 186
+KIVMVLAATNFPWDIDEALRRRLEKRIYIPLP FESRK LI INL+ V++A DV+IDEV
Sbjct: 229 KKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPAFESRKELIRINLKGVEVAPDVDIDEV 408
Query: 187 ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPVAMCDFVEALVK 366
ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDP+AMCDF EAL K
Sbjct: 409 ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPIAMCDFEEALSK 588
Query: 367 VQKSVSPSDIEKHEKWMAEFGSA* 438
VQ+SVS +DIE+HEKW AEFGSA*
Sbjct: 589 VQRSVSAADIERHEKWFAEFGSA* 660
Score = 160 bits (345), Expect = 7e-039
Identities = 68/142 (47%), Positives = 91/142 (64%)
Frame = -1 / -3
Query: 432 RPKLSHPLLMLLYV*RRDTLLNLHQSLHKVAHGHRVLGYLVL*HVLDLVAGLACYLAPHA 253
R KL P M LY+ R+T LN + K+ HG+ + L+L*H+L L+ L C L+PHA
Sbjct: 654 RTKLCKPFFMALYISSRNTTLNFAKGFFKITHGNGIFSNLIL*HILYLITSLPCNLSPHA 475
Query: 252 IH*GIAANVCQIISTIAFCPPSNLVDVNICGYLNCSKINVDKCLSTFKIWKWNINTLLQP 73
IH I N+C IIST++ C SN ++VNI YLN ++N DK L+ K K IN+LL P
Sbjct: 474 IHGCIPTNIC*IISTVSLCAASNFINVNIWCYLNTLQVNADKLLTRLKSRKRYINSLL*P 295
Query: 72 SPQCLINIPWKICSSQNHNNFL 7
SP+ I+IPW+ICS Q+H FL
Sbjct: 294 SPESFIDIPWEICSCQHHYYFL 229
Score = 155 bits (332), Expect(2) = 3e-040
Identities = 74/104 (71%), Positives = 83/104 (79%)
Frame = -2 / -1
Query: 434 ADPNSAIHFSCFSMSEGETLF*TFTRASTKSHMATGSLAISSFDMFLISSRVLPAILRLM 255
A+PNSA HFS S+S ETL *T RAS+KSHMA GSLAISSFD+F ISSRV PAI RLM
Sbjct: 656 AEPNSANHFSWRSISAAETLR*TLLRASSKSHMAMGSLAISSFDIFFISSRVFPAIFRLM 477
Query: 254 PFIEASRQTFVRSSPL*PSVLRATSSMLTSAAI*TVLRLMLISA 123
PF++ASRQTFV+SSPL PSV RATSSM TS A T +LM IS+
Sbjct: 476 PFMDASRQTFVKSSPLYPSVRRATSSMSTSGATSTPFKLMRISS 345
Score = 30.9 bits (61), Expect(2) = 3e-040
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = -2 / -1
Query: 47 HGKFVAARTITIFW 6
HGKFVAA TITIF+
Sbjct: 269 HGKFVAASTITIFF 228
>gb|DR743729.1|DR743729 RTCU1_17_G02.g1_A029 Roots plus added copper Pinus taeda cDNA clone
RTCU1_17_G02_A029 5', mRNA sequence
Length = 712
Score = 297 bits (644), Expect = 4e-080
Identities = 125/144 (86%), Positives = 135/144 (93%)
Frame = +1 / +1
Query: 7 QKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININLRTVQIAADVNIDEV 186
++IVMVLAATNFPWDIDEALRRRLEKRIYIPLP FESRK LI INL+ V++A DV+IDEV
Sbjct: 13 EEIVMVLAATNFPWDIDEALRRRLEKRIYIPLPAFESRKELIRINLKGVEVAPDVDIDEV 192
Query: 187 ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPVAMCDFVEALVK 366
ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDP+AMCDF EAL K
Sbjct: 193 ARRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPIAMCDFEEALSK 372
Query: 367 VQKSVSPSDIEKHEKWMAEFGSA* 438
VQ+SVS +DIE+HEKW AEFGSA*
Sbjct: 373 VQRSVSAADIERHEKWFAEFGSA* 444
Score = 159 bits (341), Expect = 3e-038
Identities = 67/141 (47%), Positives = 90/141 (63%)
Frame = -1 / -2
Query: 432 RPKLSHPLLMLLYV*RRDTLLNLHQSLHKVAHGHRVLGYLVL*HVLDLVAGLACYLAPHA 253
R KL P M LY+ R+T LN + K+ HG+ + L+L*H+L L+ L C L+PHA
Sbjct: 438 RTKLCKPFFMALYISSRNTTLNFAKGFFKITHGNGIFSNLIL*HILYLITSLPCNLSPHA 259
Query: 252 IH*GIAANVCQIISTIAFCPPSNLVDVNICGYLNCSKINVDKCLSTFKIWKWNINTLLQP 73
IH I N+C IIST++ C SN ++VNI YLN ++N DK L+ K K IN+LL P
Sbjct: 258 IHGCIPTNIC*IISTVSLCAASNFINVNIWCYLNTLQVNADKLLTRLKSRKRYINSLL*P 79
Query: 72 SPQCLINIPWKICSSQNHNNF 10
SP+ I+IPW+ICS Q+H F
Sbjct: 78 SPESFIDIPWEICSCQHHYYF 16
Score = 155 bits (332), Expect(2) = 2e-039
Identities = 74/104 (71%), Positives = 83/104 (79%)
Frame = -2 / -3
Query: 434 ADPNSAIHFSCFSMSEGETLF*TFTRASTKSHMATGSLAISSFDMFLISSRVLPAILRLM 255
A+PNSA HFS S+S ETL *T RAS+KSHMA GSLAISSFD+F ISSRV PAI RLM
Sbjct: 440 AEPNSANHFSWRSISAAETLR*TLLRASSKSHMAMGSLAISSFDIFFISSRVFPAIFRLM 261
Query: 254 PFIEASRQTFVRSSPL*PSVLRATSSMLTSAAI*TVLRLMLISA 123
PF++ASRQTFV+SSPL PSV RATSSM TS A T +LM IS+
Sbjct: 260 PFMDASRQTFVKSSPLYPSVRRATSSMSTSGATSTPFKLMRISS 129
Score = 27.6 bits (54), Expect(2) = 2e-039
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = -2 / -3
Query: 47 HGKFVAARTITI 12
HGKFVAA TITI
Sbjct: 53 HGKFVAASTITI 18
>gb|CF397297.1|CF397297 RTDS3_2_D11.g1_A022 Drought-stressed loblolly pine roots DS3 Pinus
taeda cDNA clone RTDS3_2_D11_A022 5', mRNA sequence
Length = 799
Score = 177 bits (380), Expect = 1e-043
Identities = 74/84 (88%), Positives = 79/84 (94%)
Frame = +1 / +1
Query: 7 QKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININLRTVQIAADVNIDEV 186
+KIVMVLAATNFPWDIDEALRRRLEKRIYIPLP FESRK LI INL+ V++A DV+IDEV
Sbjct: 547 KKIVMVLAATNFPWDIDEALRRRLEKRIYIPLPAFESRKELIRINLKGVEVAPDVDIDEV 726
Query: 187 ARRTEGYSGDDLTNVCRDASMNGM 258
ARRTEGYSGDDLTNVCRDASMNGM
Sbjct: 727 ARRTEGYSGDDLTNVCRDASMNGM 798
Score = 104 bits (221), Expect = 9e-022
Identities = 44/84 (52%), Positives = 57/84 (67%)
Frame = -1 / -2
Query: 258 HAIH*GIAANVCQIISTIAFCPPSNLVDVNICGYLNCSKINVDKCLSTFKIWKWNINTLL 79
HAIH I N+C IIST++ C SN ++VNI YLN ++N DK L+ K K IN+LL
Sbjct: 798 HAIHGCIPTNIC*IISTVSLCAASNFINVNIWCYLNTLQVNADKLLTRLKSRKRYINSLL 619
Query: 78 QPSPQCLINIPWKICSSQNHNNFL 7
PSP+ I+IPW+ICS Q+H FL
Sbjct: 618 *PSPESFIDIPWEICSCQHHYYFL 547
>gb|CF397205.1|CF397205 RTDS3_2_D11.b2_A022 Drought-stressed loblolly pine roots DS3 Pinus
taeda cDNA clone RTDS3_2_D11_A022 3', mRNA sequence
Length = 788
Score = 144 bits (308), Expect = 9e-034
Identities = 60/68 (88%), Positives = 64/68 (94%)
Frame = +1 / +2
Query: 235 RDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPVAMCDFVEALVKVQKSVSPSDIEKHEKW 414
RDASMNGMRRKIAGKTRDEIKNMSKDEIAKDP+AMCDF EAL KVQ+SVS +DIE+HEKW
Sbjct: 2 RDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPIAMCDFEEALSKVQRSVSAADIERHEKW 181
Query: 415 MAEFGSA* 438
AEFGSA*
Sbjct: 182 FAEFGSA* 205
Score = 107 bits (228), Expect = 1e-022
Identities = 49/67 (73%), Positives = 55/67 (82%)
Frame = -2 / -3
Query: 434 ADPNSAIHFSCFSMSEGETLF*TFTRASTKSHMATGSLAISSFDMFLISSRVLPAILRLM 255
A+PNSA HFS S+S ETL *T RAS+KSHMA GSLAISSFD+F ISSRV PAI RLM
Sbjct: 201 AEPNSANHFSWRSISAAETLR*TLLRASSKSHMAMGSLAISSFDIFFISSRVFPAIFRLM 22
Query: 254 PFIEASR 234
PF++ASR
Sbjct: 21 PFMDASR 1
>gb|CF400358.1|CF400358 RTWW1_4_D05.g1_A015 Well-watered loblolly pine roots WW1 Pinus
taeda cDNA clone RTWW1_4_D05_A015 5', mRNA sequence
Length = 773
Score = 66.6 bits (139), Expect(3) = 9e-025
Identities = 24/43 (55%), Positives = 33/43 (76%)
Frame = +1 / -3
Query: 16 VMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININL 144
V+VL ATN PW +D A++RR EKRIYIPLP E+RK + +++
Sbjct: 555 VLVLGATNIPWALDNAIKRRFEKRIYIPLPGLEARKRMFELHV 427
Score = 46.0 bits (94), Expect(3) = 9e-025
Identities = 15/40 (37%), Positives = 29/40 (72%)
Frame = +1 / -3
Query: 313 EIAKDPVAMCDFVEALVKVQKSVSPSDIEKHEKWMAEFGS 432
E+ + P+ M DF+++L V+ +V+ +DI+KH++W E G+
Sbjct: 183 ELHEPPLRMADFIKSLASVRPTVTQADIKKHDEWTQESGN 64
Score = 38.6 bits (78), Expect(3) = 9e-025
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = +1 / -3
Query: 172 NIDEVARRTEGYSGDDLTNVCRDASMNGMRR 264
N E+A +TEGYSG D+ + RDA M +R+
Sbjct: 396 NYRELAEQTEGYSGSDIAIIVRDALMQPVRK 304
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 396,958,485
Number of Sequences: 355925
Number of extensions: 5767619
Number of successful extensions: 68091
Number of sequences better than 1.0e-020: 5
length of database: 72,425,745
effective HSP length: 52
effective length of database: 53,917,645
effective search space used: 10406105485
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)