TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161254.2.1
(1192 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR167393.1|DR167393 RTPHOS1_18_B12.g1_A029 Roots minus p... 227 2e-064
gb|AW011359.1|AW011359 ST19H02 Pine TriplEx shoot tip libra... 121 6e-050
gb|BI397974.1|BI397974 NXPV_112_H04_F NXPV (Nsf Xylem Plani... 129 3e-029
>gb|DR167393.1|DR167393 RTPHOS1_18_B12.g1_A029 Roots minus phosphorous Pinus taeda cDNA clone
RTPHOS1_18_B12_A029 5', mRNA sequence
Length = 785
Score = 227 bits (491), Expect(3) = 2e-064
Identities = 87/126 (69%), Positives = 99/126 (78%)
Frame = +1 / +1
Query: 814 ASATITVSGTSNWTKVEQKLVAKGTNRTSRLQITTNKKGVVWLDQVSLMPEDTYKGHGFR 993
+S + S NWTK E L A T+ +RL +T++KKG+VW DQVS MP DT+KGHGFR
Sbjct: 241 SSIIASSSEIQNWTKKEFILKATATDHYARLSLTSSKKGIVWFDQVSAMPVDTFKGHGFR 420
Query: 994 TELISMLLDLKPRFLRFPGGCFVEGDWLRNAFRWRESIGPWEERPGHFGDVWHYWTDDGL 1173
L SML DLKP F+RFPGGCFVEG WLRNAF WR+SIGPWEERPGHFGDVW YWTDDGL
Sbjct: 421 KGLASMLADLKPAFIRFPGGCFVEGVWLRNAFPWRQSIGPWEERPGHFGDVWGYWTDDGL 600
Query: 1174 GYYEFL 1191
G+YEFL
Sbjct: 601 GFYEFL 618
Score = 158 bits (340), Expect(2) = 2e-049
Identities = 77/119 (64%), Positives = 86/119 (72%)
Frame = -3 / -1
Query: 1190 RNS*YPSPSSVQ*CQTSPKCPGLSSHGPIDSLHLNAFLSQSPSTKQPPGNLKNRGFKSKS 1011
RNS* PSPSSVQ* TSPK PGLSSHGPID L NAFLS +PSTKQPPGNL GF+S S
Sbjct: 617 RNS*NPSPSSVQ*PHTSPK*PGLSSHGPIDCLQGNAFLSHTPSTKQPPGNLIKAGFRSAS 438
Query: 1010 MDISSVRKPCPLYVSSGMSDT*SSQTTPFLFVVI*SLDVLLVPLATSFCSTFVQFDVPE 834
MD S RKPCPL VS+GM++T*S+QT PF +V SL V +A S FVQF + E
Sbjct: 437 MDASPFRKPCPLNVSTGMAET*SNQTIPFFELVKESLA**SVAVAFKMNSFFVQF*ISE 261
Score = 122 bits (262), Expect(2) = 8e-031
Identities = 51/97 (52%), Positives = 66/97 (68%)
Frame = -2 / -3
Query: 1191 KELIISKPIISPVMPNIPEVSRPLFPWTN*FSPSECIS*PVTFYKTTSRKSQESWFQIQK 1012
K+LI KPIISP+ P I +++RPL PW+N* P +CIS P FYKT + +S +SWFQI K
Sbjct: 618 KKLIKPKPIISPIAPYITKMTRPLLPWSN*LPPGKCISKPYPFYKTATWESDKSWFQISK 439
Query: 1011 HGYKFCAETMSLVCVFRHE*YLIKPNNSFFVRCYLKP 901
HG ++TMSL C++RH LIKPN + C KP
Sbjct: 438 HGC*SFSKTMSLECIYRHGRNLIKPNYTLLRACQGKP 328
Score = 102 bits (217), Expect = 6e-021
Identities = 57/119 (47%), Positives = 69/119 (57%)
Frame = +2 / +2
Query: 836 LAHQIGQKWSRSWLLKEPIEHQGFK*QRTKKELFGLIKYHSCLKTHTRDMVSAQNLYPCF 1015
L + GQK S L++ I GF *Q ++ FGLIK+ CL H+RDMV ++ +PC
Sbjct: 263 LKFKTGQKRSSF*KLQQQIIMLGFP*QARRRV*FGLIKFLPCL*IHSRDMVFEKD*HPCL 442
Query: 1016 WI*NHDS*DFLEVVL*KVTG*EMHSDGENQLVHGKRGLDTSGMFGITGLMMGLDIMSSF 1192
I*N D VL*K G EMH G NQL HG+RGL M+G GLMMGL MS F
Sbjct: 443 LI*NQLLSDSQVAVL*KGYGLEMHFPGGNQLDHGRRGLVILVMYGAIGLMMGLGFMSFF 619
Score = 97.3 bits (206), Expect(2) = 9e-041
Identities = 36/78 (46%), Positives = 58/78 (74%)
Frame = +1 / +1
Query: 655 DECPAGGVGVYNPGFWGMNIEDGKAYNLVMYVQSPETKDLTVSLTSSDGLQNLASATITV 834
++CP GGVGVYNPG+WGMNIE+G++Y++V++V+S +L++S TSSDGL LA+++I
Sbjct: 76 NQCPVGGVGVYNPGYWGMNIEEGESYHVVLWVRSTGPVNLSISFTSSDGLNILAASSIIA 255
Query: 835 SGTSNWTKVEQKLVAKGT 888
S + +++ + K T
Sbjct: 256 SSSEIQNWTKKEFILKAT 309
Score = 90.9 bits (192), Expect(2) = 9e-041
Identities = 40/70 (57%), Positives = 47/70 (67%)
Frame = +3 / +3
Query: 936 LA*SSITHA*RHIQGTWFPHRTYIHAFGFETTILEISWRLFCRR*LAKKCIQMERINWSM 1115
L *SS HA R+IQGTWF RT IHA ET+ +I LFCRR +A KCI +E INW+M
Sbjct: 363 LV*SSFCHACRYIQGTWFSKRTSIHAC*SETSFYQIPRWLFCRRGMA*KCISLEAINWTM 542
Query: 1116 GREAWTLRGC 1145
G EAW+ C
Sbjct: 543 GGEAWSFW*C 572
Score = 58.8 bits (122), Expect(2) = 2e-049
Identities = 30/57 (52%), Positives = 36/57 (63%)
Frame = -3 / -1
Query: 830 VIVAEAKFCNPSELVNETVRSFVSGD*TYITRL*AFPSSMFMPQKPGL*TPTPPAGH 660
+I+ AK +PSE V E R D T+ T * PSS+F+PQ PGL*TPTPP GH
Sbjct: 251 MILEAAKMLSPSEDVKEIERLTGPVDLTHKTT**DSPSSIFIPQ*PGL*TPTPPTGH 81
Score = 35.0 bits (70), Expect(2) = 8e-031
Identities = 11/29 (37%), Positives = 21/29 (72%)
Frame = -2 / -3
Query: 741 NEIVGLPIFYVHAPKTWVINTNTTSWTLI 655
N ++G +F +H+P T +IN+N+ + TL+
Sbjct: 162 NNMIGFTLFNIHSPVTRIINSNSPNRTLV 76
Score = 35.0 bits (70), Expect(3) = 2e-064
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +1 / +1
Query: 598 DRTSCFSRNIVALRMEVLCD 657
DR+S F N +ALRME+LCD
Sbjct: 4 DRSSLFELNKIALRMEILCD 63
Score = 23.5 bits (45), Expect(3) = 2e-064
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3 / +3
Query: 660 VSSWWCWCL*PRFLGHEHRR 719
VS W W L* LG+E+ R
Sbjct: 81 VSCWGSWSL*SWLLGNEY*R 140
>gb|AW011359.1|AW011359 ST19H02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST19H02, mRNA sequence
Length = 601
Score = 121 bits (258), Expect(2) = 6e-050
Identities = 42/48 (87%), Positives = 43/48 (89%)
Frame = +1 / +3
Query: 1042 FPGGCFVEGDWLRNAFRWRESIGPWEERPGHFGDVWHYWTDDGLGYYE 1185
FPGGCFVEG W RNAF WR+SIGPWEERPGHFGDVW YWTDDGLG YE
Sbjct: 270 FPGGCFVEGVWHRNAFPWRQSIGPWEERPGHFGDVWGYWTDDGLGLYE 413
Score = 97.7 bits (207), Expect(2) = 6e-050
Identities = 42/82 (51%), Positives = 52/82 (63%)
Frame = +1 / +2
Query: 814 ASATITVSGTSNWTKVEQKLVAKGTNRTSRLQITTNKKGVVWLDQVSLMPEDTYKGHGFR 993
+S + S NWTK E L A T+ +RL +T++KKG+VW DQVS MP T+KGHGFR
Sbjct: 41 SSIIASSSEIQNWTKKEFILKATATDHYARLSLTSSKKGIVWFDQVSAMPVXTFKGHGFR 220
Query: 994 TELISMLLDLKPRFLRFPGGCF 1059
L SML DLKP F+ P F
Sbjct: 221 KGLASMLADLKPAFIXIPRWLF 286
Score = 85.4 bits (180), Expect(2) = 4e-030
Identities = 38/48 (79%), Positives = 39/48 (81%)
Frame = -3 / -1
Query: 1184 S*YPSPSSVQ*CQTSPKCPGLSSHGPIDSLHLNAFLSQSPSTKQPPGN 1041
S* PSPSSVQ* TSPK PGLSSHGPID L NAFL +PSTKQPPGN
Sbjct: 412 S*SPSPSSVQ*PHTSPK*PGLSSHGPIDCLQGNAFLCHTPSTKQPPGN 269
Score = 67.0 bits (140), Expect(2) = 4e-030
Identities = 34/65 (52%), Positives = 42/65 (64%)
Frame = -3 / -2
Query: 1028 GFKSKSMDISSVRKPCPLYVSSGMSDT*SSQTTPFLFVVI*SLDVLLVPLATSFCSTFVQ 849
GF+S SMD S RKPCPL V +GM++T*S+QT PF +V SL V +A S FVQ
Sbjct: 255 GFRSASMDASPFRKPCPLNVXTGMAET*SNQTIPFFELVKESLA**SVAVAFKMNSFFVQ 76
Query: 848 FDVPE 834
F + E
Sbjct: 75 F*ISE 61
>gb|BI397974.1|BI397974 NXPV_112_H04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_112_H04 5' similar to Arabidopsis
thaliana sequence At3g10740 putative
alpha-L-arabinofuranosidase see
http://mips.gsf.de/proj/thal/db/index.html, mRNA
sequence
Length = 332
Score = 129 bits (277), Expect = 3e-029
Identities = 54/83 (65%), Positives = 63/83 (75%)
Frame = +1 / +2
Query: 409 QLARKIPETLFGIFFEEINHAGAGGIWAELVSNRGFEAGGSHTPSNIDPWSIIGDDSSVF 588
Q R+IP TLFG+FF INHAGAGG+WAELVSN FEAGG +TPSNI S D+ SV
Sbjct: 68 QPGRQIPSTLFGLFFXXINHAGAGGLWAELVSNXXFEAGGPNTPSNIXXXSPXXDEXSVL 247
Query: 589 VATDRTSCFSRNIVALRMEVLCD 657
+ATDR+S F N +ALRME+LCD
Sbjct: 248 LATDRSSLFELNKIALRMEILCD 316
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 711,847,397
Number of Sequences: 355925
Number of extensions: 11733774
Number of successful extensions: 64047
Number of sequences better than 1.0e-020: 5
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 18371669960
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)