TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1724818.2.1
(1089 letters)
Database: Pinus_nucl_with_EST.fasta
355,925 sequences; 217,277,237 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DR118536.1|DR118536 RTMG1_17_D04.g1_A029 Roots minus mag... 245 6e-074
dbj|BD267264.1| Compositions isolated from plant cells and ... 155 5e-037
dbj|BD267344.1| Compositions isolated from plant cells and ... 68 2e-022
>gb|DR118536.1|DR118536 RTMG1_17_D04.g1_A029 Roots minus magnesium Pinus taeda cDNA clone
RTMG1_17_D04_A029 5', mRNA sequence
Length = 680
Score = 245 bits (530), Expect(3) = 6e-074
Identities = 98/175 (56%), Positives = 128/175 (73%)
Frame = +3 / +3
Query: 423 DLALLHLNSNRFCGVXXXXXXXXXXXXXXXXSNNRFVGAFPAVVLALPSLRYLDLRFNDF 602
DLAL H+N+NRFCG+ SNNRF G FP VV+ LPSL+YLD+RFN+F
Sbjct: 156 DLALFHINTNRFCGIVPHSFKNMTLLYELDISNNRFAGPFPKVVIGLPSLKYLDIRFNEF 335
Query: 603 EGPIPPPLFDRPLDAILLNSNRLRGPIPANLGNSPASVLVLAHNRLGGCIPPSIGRMAAT 782
EG +P LFD+ LDAI +N+NR IP+NLGNSP SV+V A+N L GC+PPS+G+MAAT
Sbjct: 336 EGKLPTKLFDKDLDAIFVNNNRFHLEIPSNLGNSPVSVVVFANNNLKGCVPPSLGKMAAT 515
Query: 783 LNEIVLIDDDLTGCVPPQVGLLTKLTVFDVSGNHLQGPLPPAVGGMAVLQQLNVA 947
LNEI+ ++++L+ C+P ++GLL LTVFD S N L GPLP ++G M L+QL+VA
Sbjct: 516 LNEIIFLNNNLSACLPSEIGLLKNLTVFDASFNKLVGPLPDSIGQMDSLEQLDVA 680
Score = 43.2 bits (88), Expect(3) = 6e-074
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +1 / +3
Query: 271 TSNWAGPNVCAYNGIYCAPRPSDGALAV 354
T NW GPNVC Y G++C+ P + ++ V
Sbjct: 3 TGNWVGPNVCNYTGVFCSAPPYNKSILV 86
Score = 29.0 bits (57), Expect(3) = 6e-074
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3 / +3
Query: 369 NHADIAGHIPADLPL 413
NHADIAG++P +L L
Sbjct: 105 NHADIAGYLPTELGL 149
>dbj|BD267264.1| Compositions isolated from plant cells and utilization of the same
in modifying plant cell signal transduction
Length = 551
Score = 155 bits (333), Expect = 5e-037
Identities = 60/108 (55%), Positives = 83/108 (76%)
Frame = +3 / +3
Query: 663 NRLRGPIPANLGNSPASVLVLAHNRLGGCIPPSIGRMAATLNEIVLIDDDLTGCVPPQVG 842
NR IP+NLGNSP SV+V A+N L GC+PPS+G+MAATLNEI+ ++++L+ C+P ++G
Sbjct: 3 NRFHLEIPSNLGNSPVSVVVFANNNLKGCVPPSLGKMAATLNEIIFLNNNLSACLPSEIG 182
Query: 843 LLTKLTVFDVSGNHLQGPLPPAVGGMAVLQQLNVAGNLLRGPVPSAVC 986
LL LTVFD S N L GPLP ++G M L+QL+VA N+L G +P ++C
Sbjct: 183 LLKNLTVFDASFNKLVGPLPDSIGQMDSLEQLDVAHNMLTGKIPQSIC 326
>dbj|BD267344.1| Compositions isolated from plant cells and utilization of the same in
modifying plant cell signal transduction
Length = 2691
Score = 68.4 bits (143), Expect(3) = 2e-022
Identities = 33/84 (39%), Positives = 45/84 (53%)
Frame = +3 / +1
Query: 516 SNNRFVGAFPAVVLALPSLRYLDLRFNDFEGPIPPPLFDRPLDAILLNSNRLRGPIPANL 695
SNN F G+ P + L +L LDL N F G +P + L A+ L +N + G IP N+
Sbjct: 1312 SNNNFTGSLPRNIGQLSNLYLLDLSSNKFTGSLPLSISHLDLQALYLYNNNISGTIPNNI 1491
Query: 696 GNSPASVLVLAHNRLGGCIPPSIG 767
S +L L++N L G IP IG
Sbjct: 1492 SFSDIQILDLSNNNLEGSIPKLIG 1563
Score = 58.8 bits (122), Expect(3) = 2e-022
Identities = 21/59 (35%), Positives = 39/59 (66%)
Frame = +3 / +1
Query: 798 LIDDDLTGCVPPQVGLLTKLTVFDVSGNHLQGPLPPAVGGMAVLQQLNVAGNLLRGPVP 974
L +++L G +P +G L + + + SGN L+GP+P + G ++ L+QL+++ N L G +P
Sbjct: 1978 LSNNNLVGQIPASIGTLRDMRLLNFSGNQLEGPIPASFGNISTLEQLDLSKNNLNGSIP 2154
Score = 25.4 bits (49), Expect(3) = 2e-022
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +1 / +1
Query: 292 NVCAYNGIYCAP 327
NVC +NGI C P
Sbjct: 232 NVCLWNGISCRP 267
Database: Pinus_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:45 PM
Number of letters in database: 217,277,237
Number of sequences in database: 355,925
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 282,420,828
Number of Sequences: 355925
Number of extensions: 3139767
Number of successful extensions: 22303
Number of sequences better than 1.0e-020: 3
length of database: 72,425,745
effective HSP length: 53
effective length of database: 53,561,720
effective search space used: 16550571480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)