TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS9f07.yg.2.1
(394 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY557602.1| Medicago truncatula cultivar Jemalong core a... 132 4e-052
gb|AC175027.3| Medicago truncatula clone mth2-102d9, WORKIN... 130 1e-050
emb|AJ416755.1|MTR416755 Medicago truncatula partial mRNA f... 80 1e-014
gb|AC133341.11| Medicago truncatula clone mth2-28i3, comple... 34 1.5
gb|AC146819.18| Medicago truncatula clone mth2-176k6, compl... 34 1.5
gb|BG645679.1|BG645679 EST507298 KV3 Medicago truncatula cD... 33 2.1
gb|CX528238.1|CX528238 s13dNF53C04AT034_516988 Aphid-Infect... 33 2.1
gb|AC146551.24| Medicago truncatula clone mth2-8m1, WORKING... 26 2.2
gb|CG929222.1|CG929222 MBEIV61TF mth2 Medicago truncatula g... 31 7.4
emb|CR482287.1| mth2-146E21FM1 BAC end, cultivar Jemalong A... 31 7.4
gb|CB892308.1|CB892308 EST649277 KV3 Medicago truncatula cD... 31 7.4
gb|AC140850.20| Medicago truncatula clone mth2-11i23, compl... 31 7.4
gb|AC146705.11| Medicago truncatula clone mth2-101f3, compl... 31 7.4
>gb|AY557602.1| Medicago truncatula cultivar Jemalong core alpha
1,3-fucosyltransferase (FUT2) gene, complete cds
Length = 3124
Score = 132 bits (283), Expect(2) = 4e-052
Identities = 49/74 (66%), Positives = 61/74 (82%)
Frame = -1 / +1
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSSKKGTVYHLF 215
KY+GPSDSFKAL+DMAAVHSSCRLCIH+A+K KEE++P F RPC C+ TVYH++
Sbjct: 2191 KYEGPSDSFKALVDMAAVHSSCRLCIHLASKSREKEEKSPDFKRRPCKCTRGSETVYHIY 2370
Query: 214 IRERGRFKSESIYM 173
+RERG F+ ESIY+
Sbjct: 2371 VRERGTFEMESIYL 2412
Score = 95.9 bits (203), Expect(2) = 4e-052
Identities = 38/56 (67%), Positives = 46/56 (82%)
Frame = -1 / +2
Query: 169 SGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
S LTL + ++AVL KF SLNHVPVWK ERP ++GGD+LK+Y+IYP GLTQRQAL
Sbjct: 2870 SSNLTLESFKTAVLTKFTSLNHVPVWKPERPEILKGGDELKVYKIYPAGLTQRQAL 3037
Score = 64.8 bits (135), Expect(2) = 4e-015
Identities = 30/47 (63%), Positives = 35/47 (74%)
Frame = +3 / -3
Query: 252 LQGRFVNFGVLSSFK*ILVAICIQRRQDE*TAAMSIRALKESDGPSY 392
LQGR + G+ SSF + A +QRRQDE TAA+S RALKESDGPSY
Sbjct: 2333 LQGRLLKSGLFSSFSLLFEAK*MQRRQDECTAAISTRALKESDGPSY 2193
Score = 39.1 bits (79), Expect(2) = 4e-007
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = +2 / -2
Query: 257 RSICKFWSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTIIL 394
RS + W+ L L + K R*M CH+ K E IRW IL
Sbjct: 2328 RSPLEVWALLFLLSTL*GQVNAKAAR*MYSCHIHKGLEGIRWPFIL 2191
Score = 39.1 bits (79), Expect(2) = 4e-015
Identities = 22/57 (38%), Positives = 31/57 (54%)
Frame = +2 / -1
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARS 172
+SL LC V V Q I T F LPYRN+++G + ++ +G Q *+ARS
Sbjct: 3037 KSLPLCQARRVYFVNLQFITTF*NFRSFRLPYRNMIQGRELRKNSSLEGLQS*IARS 2867
Score = 37.7 bits (76), Expect(2) = 6e-006
Identities = 20/48 (41%), Positives = 27/48 (56%)
Frame = -3 / +1
Query: 158 NTGSLGICSAR*I*VPQPRSCMEG*KATEY*RWG*PETIQNLPSRSNT 15
N+G L C + + VP+ SCME *K RW * E +QN+P +T
Sbjct: 2881 NSGVLQDCCSYEVHVPESCSCMEA*KT*NSKRW**IEGLQNIPCGLDT 3024
Score = 37.7 bits (76), Expect(2) = 4e-007
Identities = 20/52 (38%), Positives = 23/52 (44%)
Frame = +3 / -2
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPD 170
CV+P G IL +F FHTGT RD+N TA K V D
Sbjct: 3024 CVKPAGYIL*TFNSSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLD 2869
Score = 35.0 bits (70), Expect(2) = 6e-006
Identities = 18/48 (37%), Positives = 25/48 (52%)
Frame = -2 / +2
Query: 393 SMMVHLILSKLLSTWQRFIHLVVFVYILLPRFI*RRKGLQNLQIDLVA 250
SM HLI S+ L WQ +IHL F + R R++ Q + DL +
Sbjct: 2192 SMKGHLIPSRPLWIWQLYIHLAAFAFTWPQRVERRKRRAQTSRGDLAS 2335
Score = 34.1 bits (68), Expect(2) = 0.001
Identities = 20/56 (35%), Positives = 28/56 (50%)
Frame = -2 / +3
Query: 171 DRAS*HWEPWNLQCSVNLGPSTTFLYGRMKGHRVLEVGMT*NYTEFTQSV*HNVRL 4
D A * W P L + P FLYG +K + +V M * +T++T *H +L
Sbjct: 2868 DLAI*LWSPSRLLFLRSSRP*IMFLYGSLKDLKF*KVVMN*RFTKYTLRA*HRGKL 3035
Score = 30.9 bits (61), Expect(2) = 0.001
Identities = 20/74 (27%), Positives = 41/74 (55%)
Frame = -3 / +3
Query: 392 V*WSI*FFQSSYRHGSGSFILSSLYTYCYQDSFKGGKDSKIYKSTL*LFQQKGDSLPLVY 213
V* +I*F Q +GS +FIL L++ ++ +G ++ ++ ++TL + + + + +
Sbjct: 2193 V*RAI*FLQGPCGYGSCTFILPPLHSLGLKE*REGREEPRLQEATLQVHSRVRNRISYLC 2372
Query: 212 PRERAV*VREHLYE 171
R+ +* HL E
Sbjct: 2373 ERKGNI*DGVHLLE 2414
>gb|AC175027.3| Medicago truncatula clone mth2-102d9, WORKING DRAFT SEQUENCE, 16
unordered pieces
Length = 124356
Score = 130 bits (278), Expect(2) = 1e-050
Identities = 49/75 (65%), Positives = 61/75 (81%)
Frame = -1 / -1
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSSKKGTVYHLF 215
KY+GPSDSFKAL+DMAAVHSSCRLCIH+A+K KEE++P F R C C+ TVYH++
Sbjct: 93006 KYEGPSDSFKALVDMAAVHSSCRLCIHLASKSREKEEKSPDFKKRLCKCTRGSETVYHIY 92827
Query: 214 IRERGRFKSESIYMR 170
+RERG F+ ESIY+R
Sbjct: 92826 VRERGTFEMESIYLR 92782
Score = 98.2 bits (208), Expect(2) = 1e-050
Identities = 39/57 (68%), Positives = 47/57 (82%)
Frame = -1 / -2
Query: 172 RSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
RS LTL + ++AVL KF SLNHVPVWK ERP ++GGD+LK+Y+IYP GLTQRQAL
Sbjct: 92330 RSSNLTLESFKTAVLTKFTSLNHVPVWKPERPEILKGGDELKVYKIYPAGLTQRQAL 92160
Score = 64.8 bits (135), Expect(2) = 1e-013
Identities = 30/47 (63%), Positives = 35/47 (74%)
Frame = +3 / +2
Query: 252 LQGRFVNFGVLSSFK*ILVAICIQRRQDE*TAAMSIRALKESDGPSY 392
LQ RF+ G+ SSF + A +QRRQDE TAA+S RALKESDGPSY
Sbjct: 92864 LQSRFLKSGLFSSFSLLFEAK*MQRRQDECTAAISTRALKESDGPSY 93004
Score = 40.0 bits (81), Expect(2) = 2e-006
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = +2 / +1
Query: 257 RSICKFWSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTIIL 394
+S+ + W+ L L + K R*M CH+ K E IRW IL
Sbjct: 92869 KSLLEVWALLFLLSTL*GQVNAKAAR*MYSCHIHKGLEGIRWPFIL 93006
Score = 39.6 bits (80), Expect(2) = 1e-013
Identities = 21/53 (39%), Positives = 24/53 (45%)
Frame = +3 / +1
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPDL 173
CV+P G IL +F FHTGT RD+N TA K V DL
Sbjct: 92173 CVKPAGYIL*TFNSSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDL 92331
Score = 39.1 bits (79), Expect(2) = 2e-006
Identities = 22/57 (38%), Positives = 31/57 (54%)
Frame = +2 / +3
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARS 172
+SL LC V V Q I T F LPYRN+++G + ++ +G Q *+ARS
Sbjct: 92160 KSLPLCQARRVYFVNLQFITTF*NFRSFRLPYRNMIQGRELRKNSSLEGLQS*IARS 92330
Score = 37.7 bits (76), Expect(2) = 3e-004
Identities = 20/48 (41%), Positives = 27/48 (56%)
Frame = -3 / -1
Query: 158 NTGSLGICSAR*I*VPQPRSCMEG*KATEY*RWG*PETIQNLPSRSNT 15
N+G L C + + VP+ SCME *K RW * E +QN+P +T
Sbjct: 92316 NSGVLQDCCSYEVHVPESCSCMEA*KT*NSKRW**IEGLQNIPCGLDT 92173
Score = 34.1 bits (68), Expect(2) = 0.012
Identities = 20/56 (35%), Positives = 28/56 (50%)
Frame = -2 / -3
Query: 171 DRAS*HWEPWNLQCSVNLGPSTTFLYGRMKGHRVLEVGMT*NYTEFTQSV*HNVRL 4
D A * W P L + P FLYG +K + +V M * +T++T *H +L
Sbjct: 92329 DLAI*LWSPSRLLFLRSSRP*IMFLYGSLKDLKF*KVVMN*RFTKYTLRA*HRGKL 92162
Score = 34.1 bits (68), Expect(2) = 3e-004
Identities = 17/48 (35%), Positives = 24/48 (50%)
Frame = -2 / -2
Query: 393 SMMVHLILSKLLSTWQRFIHLVVFVYILLPRFI*RRKGLQNLQIDLVA 250
SM HLI S+ L WQ +IHL F + R R++ Q + D +
Sbjct: 93005 SMKGHLIPSRPLWIWQLYIHLAAFAFTWPQRVERRKRRAQTSRSDFAS 92862
Score = 32.2 bits (64), Expect(2) = 0.012
Identities = 20/75 (26%), Positives = 42/75 (56%)
Frame = -3 / -3
Query: 392 V*WSI*FFQSSYRHGSGSFILSSLYTYCYQDSFKGGKDSKIYKSTL*LFQQKGDSLPLVY 213
V* +I*F Q +GS +FIL L++ ++ +G ++ ++ ++TL + + + + +
Sbjct: 93004 V*RAI*FLQGPCGYGSCTFILPPLHSLGLKE*REGREEPRLQEATLQVHSRVRNCISYLC 92825
Query: 212 PRERAV*VREHLYEI 168
R+ +* HL E+
Sbjct: 92824 ERKGNI*DGVHLLEV 92780
>emb|AJ416755.1|MTR416755 Medicago truncatula partial mRNA for alpha1,3-fucosyltransferase
(fuct3 gene)
Length = 658
Score = 80.3 bits (169), Expect = 1e-014
Identities = 30/40 (75%), Positives = 36/40 (90%)
Frame = -1 / +2
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTP 275
KY+GPSDSFKAL+DMA VHSSCRLCIH+A+K KEE++P
Sbjct: 536 KYEGPSDSFKALVDMAXVHSSCRLCIHLASKSREKEEKSP 655
Score = 54.2 bits (112), Expect = 9e-007
Identities = 25/39 (64%), Positives = 29/39 (74%)
Frame = +3 / -2
Query: 276 GVLSSFK*ILVAICIQRRQDE*TAAMSIRALKESDGPSY 392
G+ SSF + A +QRRQDE T A+S RALKESDGPSY
Sbjct: 654 GLFSSFSLLFEAK*MQRRQDECTXAISTRALKESDGPSY 538
Score = 36.8 bits (74), Expect = 0.16
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +2 / -1
Query: 275 WSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTIIL 394
W+ L L + K R*M CH+ K E IRW IL
Sbjct: 655 WALLFLLSTL*GQVNAKAAR*MYXCHIHKGLEGIRWPFIL 536
>gb|AC133341.11| Medicago truncatula clone mth2-28i3, complete sequence
Length = 138850
Score = 33.6 bits (67), Expect = 1.5
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +3 / +1
Query: 183 LSDLNRPLSRINKW*TVPFLLEQLQGRFVNFGVLSSFK*ILVAICIQRRQD 335
+ D+ P S++ KW PF E + G FV G+ S + +C+ + D
Sbjct: 979 IQDITIPRSKLTKWFMEPFFEELIVGCFVRVGIGRSKTGPIYRLCMVKNVD 1131
>gb|AC146819.18| Medicago truncatula clone mth2-176k6, complete sequence
Length = 127132
Score = 33.6 bits (67), Expect = 1.5
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +3 / -3
Query: 183 LSDLNRPLSRINKW*TVPFLLEQLQGRFVNFGVLSSFK*ILVAICIQRRQD 335
+ D+ P S++ KW PF E + G FV G+ S + +C+ + D
Sbjct: 37187 IQDITIPRSKLTKWFMEPFFEELIVGCFVRVGIGRSKTGPIYRLCMVKNVD 37035
>gb|BG645679.1|BG645679 EST507298 KV3 Medicago truncatula cDNA clone pKV3-47C5 5' end, mRNA
sequence
Length = 618
Score = 33.1 bits (66), Expect = 2.1
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = -1 / +2
Query: 298 HLKEERTPKFTNRPCSCSSKKGTVYHLFIRE 206
H+ +E T + T+ PC+C KG++ LF+ +
Sbjct: 170 HVLQEYTSRITSTPCTCFLMKGSLKVLFVHK 262
>gb|CX528238.1|CX528238 s13dNF53C04AT034_516988 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 619
Score = 33.1 bits (66), Expect = 2.1
Identities = 11/31 (35%), Positives = 20/31 (64%)
Frame = -1 / +1
Query: 298 HLKEERTPKFTNRPCSCSSKKGTVYHLFIRE 206
H+ +E T + T+ PC+C KG++ LF+ +
Sbjct: 280 HVLQEYTSRITSTPCTCFLMKGSLKVLFVHK 372
>gb|AC146551.24| Medicago truncatula clone mth2-8m1, WORKING DRAFT SEQUENCE, 5 unordered
pieces
Length = 129258
Score = 26.3 bits (51), Expect(2) = 2.2
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -3 / -1
Query: 338 FILSSLYTYCYQDSFKG 288
F LS Y+YCY + KG
Sbjct: 77463 FCLSEKYSYCYSNKGKG 77413
Score = 23.5 bits (45), Expect(2) = 2.2
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -1 / -3
Query: 241 KKGTVYHLFIRERGRFKSESI 179
+KG + +LFIR KS+SI
Sbjct: 77374 RKGRLLNLFIRSSNYGKSQSI 77312
>gb|CG929222.1|CG929222 MBEIV61TF mth2 Medicago truncatula genomic clone 65K2, DNA sequence
Length = 605
Score = 31.3 bits (62), Expect = 7.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1 / +1
Query: 391 YDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLK 290
Y S+S+ AA+H +CRLC H +I L+
Sbjct: 70 YKA*SESYTPPTWPAAMHQACRLCNHFVAQISLQ 171
>emb|CR482287.1| mth2-146E21FM1 BAC end, cultivar Jemalong A17 of Medicago
truncatula, genomic survey sequence
Length = 623
Score = 31.3 bits (62), Expect = 7.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1 / +1
Query: 391 YDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLK 290
Y S+S+ AA+H +CRLC H +I L+
Sbjct: 70 YKA*SESYTPPTWPAAMHQACRLCNHFVAQISLQ 171
>gb|CB892308.1|CB892308 EST649277 KV3 Medicago truncatula cDNA clone KV3-53H12, mRNA
sequence
Length = 623
Score = 31.3 bits (62), Expect = 7.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1 / +2
Query: 391 YDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLK 290
Y S+S+ AA+H +CRLC H +I L+
Sbjct: 95 YKA*SESYTPPTWPAAMHQACRLCNHFVAQISLQ 196
>gb|AC140850.20| Medicago truncatula clone mth2-11i23, complete sequence
Length = 138846
Score = 31.3 bits (62), Expect = 7.4
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -1 / -1
Query: 247 SSKKGTVYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGK 122
S + YH F+ G + + R G + GAL+S+V GK
Sbjct: 61584 SPPRNVRYHFFLYFLGYYVVYPLCFRFGSIIAGALQSSVFGK 61459
>gb|AC146705.11| Medicago truncatula clone mth2-101f3, complete sequence
Length = 120058
Score = 31.3 bits (62), Expect = 7.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1 / -3
Query: 391 YDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLK 290
Y S+S+ AA+H +CRLC H +I L+
Sbjct: 109361 YKA*SESYTPPTWPAAMHQACRLCNHFVAQISLQ 109260
Database: Mt_nucl_with_EST.fasta
Posted date: May 2, 2006 2:46 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 391,843,290
Number of Sequences: 392609
Number of extensions: 4783708
Number of successful extensions: 72204
Number of sequences better than 10.0: 18
length of database: 147,244,331
effective HSP length: 52
effective length of database: 126,828,663
effective search space used: 9892635714
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)