TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCL35a05.yg.2.1
(567 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CR954191.1| Medicago truncatula chromosome 5 clone mte1... 140 1e-036
emb|CR301510.1| mte1-2K10RM1 BAC end, cultivar Jemalong A17... 146 3e-034
gb|CX526606.1|CX526606 s13dNF36F10AT091_513724 Aphid-Infect... 131 6e-034
gb|CG945567.1|CG945567 MBEMS48TR mth2 Medicago truncatula g... 127 3e-032
gb|CG934467.1|CG934467 MBELJ71TR mth2 Medicago truncatula g... 116 5e-029
gb|CG942709.1|CG942709 MBEAH64TF mth2 Medicago truncatula g... 107 2e-026
gb|CG931676.1|CG931676 MBECQ89TR mth2 Medicago truncatula g... 105 9e-026
>emb|CR954191.1| Medicago truncatula chromosome 5 clone mte1-26e13, WORKING DRAFT
SEQUENCE
Length = 94044
Score = 140 bits (300), Expect(2) = 1e-036
Identities = 58/112 (51%), Positives = 76/112 (67%)
Frame = -3 / +1
Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296
A+SVV E V C+DG L+VAK++ YH V+L KF T+L+ ++
Sbjct: 31117 AISVVFDNAETEEVYQTCMDGFLAVAKVSAYYHLESILDDLVVSLCKFVTILDPLSPEES 31296
Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140
++ FGEDTKARMATE VFTIA YGD+IR+GWRNI+DCIL HK+GLLP ++
Sbjct: 31297 ILAFGEDTKARMATETVFTIANRYGDYIRTGWRNILDCILKFHKLGLLPAQM 31452
Score = 33.6 bits (67), Expect(2) = 1e-036
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = -3 / +1
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ Q+L ++TP+++ G + RFSQLL
Sbjct: 31525 SSSQLLSVNTPKRSSGFISRFSQLL 31599
>emb|CR301510.1| mte1-2K10RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 555
Score = 146 bits (313), Expect = 3e-034
Identities = 60/112 (53%), Positives = 78/112 (69%)
Frame = -3 / +3
Query: 475 AVSVVXXXVEXXXVLTGCIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDP 296
A+SVV E V C+DG L++AK++ +H V+L KFTTLLN S +++P
Sbjct: 207 AISVVFDHAEHEDVYQTCMDGFLAIAKISACHHLEDVLDDLVVSLCKFTTLLNPSLVEEP 386
Query: 295 VIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140
V+ FG+D KARMAT VFTIA YGD+IR+GWRNI+DCIL HK+GLLP R+
Sbjct: 387 VLAFGDDMKARMATVTVFTIANRYGDYIRTGWRNILDCILRLHKLGLLPARV 542
>gb|CX526606.1|CX526606 s13dNF36F10AT091_513724 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 613
Score = 131 bits (281), Expect(2) = 6e-034
Identities = 52/95 (54%), Positives = 69/95 (72%)
Frame = -3 / +1
Query: 424 CIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTKARMATEAV 245
C+DG L+VAK++ YH V+L KF T+L+ ++ ++ FGEDTKARMATE V
Sbjct: 16 CMDGFLAVAKVSAYYHLESILDDLVVSLCKFVTILDPLSPEESILAFGEDTKARMATETV 195
Query: 244 FTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140
FTIA YGD+IR+GWRNI+DCIL HK+GLLP ++
Sbjct: 196 FTIANRYGDYIRTGWRNILDCILKFHKLGLLPAQM 300
Score = 33.6 bits (67), Expect(2) = 6e-034
Identities = 12/25 (48%), Positives = 20/25 (80%)
Frame = -3 / +1
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ Q+L ++TP+++ G + RFSQLL
Sbjct: 373 SSSQLLSVNTPKRSSGFISRFSQLL 447
>gb|CG945567.1|CG945567 MBEMS48TR mth2 Medicago truncatula genomic clone 4H23, DNA sequence
Length = 880
Score = 127 bits (271), Expect(2) = 3e-032
Identities = 50/95 (52%), Positives = 66/95 (69%)
Frame = -3 / -2
Query: 424 CIDGXLSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTKARMATEAV 245
CIDG L++AK++ YH V L KF T+L+ +++ V+ FG+DTKARM TE V
Sbjct: 879 CIDGFLAIAKISAYYHLENVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETV 700
Query: 244 FTIATAYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140
FTIA+ YGD+IR+GWRNI+DCI H +GLLP L
Sbjct: 699 FTIASRYGDYIRTGWRNILDCIFRLHNLGLLPTHL 595
Score = 32.7 bits (65), Expect(2) = 3e-032
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -3 / -2
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ + ISTP+++ GL+ RFSQLL
Sbjct: 522 SSTHLQSISTPKRSSGLLSRFSQLL 448
>gb|CG934467.1|CG934467 MBELJ71TR mth2 Medicago truncatula genomic clone 80K22, DNA
sequence
Length = 869
Score = 116 bits (247), Expect(2) = 5e-029
Identities = 46/90 (51%), Positives = 62/90 (68%)
Frame = -3 / -3
Query: 409 LSVAKLAXXYHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTKARMATEAVFTIAT 230
L++AK++ YH V L KF T+L+ +++ V+ FG+DTKARM TE VFTIA+
Sbjct: 864 LAIAKISAYYHLKNVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIAS 685
Query: 229 AYGDHIRSGWRNIIDCILXXHKIGLLPGRL 140
YGD+IR+GWRNI+DCI H +GLLP L
Sbjct: 684 RYGDYIRTGWRNILDCIFRLHNLGLLPTHL 595
Score = 32.7 bits (65), Expect(2) = 5e-029
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -3 / -3
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ + ISTP+++ GL+ RFSQLL
Sbjct: 522 SSTHLQSISTPKRSSGLLSRFSQLL 448
>gb|CG942709.1|CG942709 MBEAH64TF mth2 Medicago truncatula genomic clone 12L8, DNA sequence
Length = 845
Score = 107 bits (228), Expect(2) = 2e-026
Identities = 43/81 (53%), Positives = 55/81 (67%)
Frame = -3 / -2
Query: 382 YHXXXXXXXXXVALSKFTTLLNTSYIDDPVIVFGEDTKARMATEAVFTIATAYGDHIRSG 203
YH V L KF T+L+ +++ V+ FG+DTKARM TE VFTIA+ YGD+IR+G
Sbjct: 835 YHLENVLDDLIVCLCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIASRYGDYIRTG 656
Query: 202 WRNIIDCILXXHKIGLLPGRL 140
WRNI+DCI H +GLLP L
Sbjct: 655 WRNILDCIFRLHNLGLLPTHL 593
Score = 32.7 bits (65), Expect(2) = 2e-026
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -3 / -2
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ + ISTP+++ GL+ RFSQLL
Sbjct: 520 SSTHLQSISTPKRSSGLLSRFSQLL 446
>gb|CG931676.1|CG931676 MBECQ89TR mth2 Medicago truncatula genomic clone 28O9, DNA sequence
Length = 770
Score = 105 bits (223), Expect(2) = 9e-026
Identities = 40/68 (58%), Positives = 52/68 (76%)
Frame = -3 / -3
Query: 343 LSKFTTLLNTSYIDDPVIVFGEDTKARMATEAVFTIATAYGDHIRSGWRNIIDCILXXHK 164
L KF T+L+ +++ V+ FG+DTKARM TE VFTIA+ YGD+IR+GWRNI+DCI H
Sbjct: 765 LCKFFTILDPLSVEESVLAFGDDTKARMVTETVFTIASRYGDYIRTGWRNILDCIFRLHN 586
Query: 163 IGLLPGRL 140
+GLLP L
Sbjct: 585 LGLLPTHL 562
Score = 32.7 bits (65), Expect(2) = 9e-026
Identities = 13/25 (52%), Positives = 19/25 (76%)
Frame = -3 / -3
Query: 76 SAPQVLPISTPRKTYGLMGRFSQLL 2
S+ + ISTP+++ GL+ RFSQLL
Sbjct: 489 SSTHLQSISTPKRSSGLLSRFSQLL 415
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 409,492,592
Number of Sequences: 392609
Number of extensions: 4847877
Number of successful extensions: 27383
Number of sequences better than 1.0e-020: 8
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 17068867290
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)