TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCL28h07.yg.2.1
(277 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC137603.16| Medicago truncatula clone mth2-14b10, compl... 179 1e-044
gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone m... 179 1e-044
gb|AC122723.6| Medicago truncatula clone mth2-5i15, complet... 179 1e-044
gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKIN... 171 2e-042
gb|AC157648.18| Medicago truncatula clone mth2-68d9, comple... 171 2e-042
gb|AC158209.13| Medicago truncatula clone mth2-154h5, compl... 152 1e-036
gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Me... 140 6e-033
gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORK... 131 2e-030
gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKI... 131 2e-030
gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Me... 128 2e-029
gb|BF004482.1|BF004482 EST432980 KV1 Medicago truncatula cD... 119 1e-026
>gb|AC137603.16| Medicago truncatula clone mth2-14b10, complete sequence
Length = 109793
Score = 179 bits (385), Expect = 1e-044
Identities = 72/85 (84%), Positives = 78/85 (91%)
Frame = -1 / -1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHYYG TLLHGGA+YR TGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 61793 ILMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHF 61614
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIVY+IFG YR ++
Sbjct: 61613 VKGIELMILLIVYQIFGNGYRSGLS 61539
>gb|AC152184.1| Medicago truncatula chromosome 7 BAC clone mte1-61c3, complete
sequence
Length = 103090
Score = 179 bits (385), Expect = 1e-044
Identities = 71/85 (83%), Positives = 79/85 (92%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHY+G TLLHGGA+YR TGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 8269 ILMQLQLAPVFFTFSLGTKTHYFGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHF 8448
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIEL++LL+VYEIF SYR A+A
Sbjct: 8449 VKGIELLVLLVVYEIFSHSYRSAVA 8523
Score = 82.6 bits (174), Expect(2) = 4e-021
Identities = 38/68 (55%), Positives = 50/68 (73%)
Frame = +2 / -2
Query: 5 NSSSV*LPKYFIHNKQNHQFNALHKMASRIQSIILSKLGMEHHKSTPCSSIFSSSM*QRG 184
NS+SV*+ K F++NK + N L K+ SRI+SII+SKLGME+HKS+ SIF +SM Q
Sbjct: 8520 NSTSV*MTKNFVYNK*YQELNTLDKVTSRIKSIIISKLGMENHKSSTRGSIFCTSMKQCP 8341
Query: 185 SVVVSFGP 208
S +VS GP
Sbjct: 8340 SKIVSLGP 8317
Score = 37.7 bits (76), Expect(2) = 4e-021
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3 / -3
Query: 195 *VLVPRENVKNTDANWSCIS 254
*VLVPRENVKNT A+ +CIS
Sbjct: 8330 *VLVPRENVKNTGASCNCIS 8271
>gb|AC122723.6| Medicago truncatula clone mth2-5i15, complete sequence
Length = 105353
Score = 179 bits (385), Expect = 1e-044
Identities = 71/85 (83%), Positives = 79/85 (92%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+LMQLQLA VFFTFSLGTKTHY+G TLLHGGA+YR TGRGFVVFHAKFA+NYRLYSRSHF
Sbjct: 79666 ILMQLQLAPVFFTFSLGTKTHYFGRTLLHGGAKYRPTGRGFVVFHAKFADNYRLYSRSHF 79845
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIEL++LL+VYEIF SYR A+A
Sbjct: 79846 VKGIELLVLLVVYEIFSHSYRSAVA 79920
Score = 82.6 bits (174), Expect(2) = 4e-021
Identities = 38/68 (55%), Positives = 50/68 (73%)
Frame = +2 / -3
Query: 5 NSSSV*LPKYFIHNKQNHQFNALHKMASRIQSIILSKLGMEHHKSTPCSSIFSSSM*QRG 184
NS+SV*+ K F++NK + N L K+ SRI+SII+SKLGME+HKS+ SIF +SM Q
Sbjct: 79917 NSTSV*MTKNFVYNK*YQELNTLDKVTSRIKSIIISKLGMENHKSSTRGSIFCTSMKQCP 79738
Query: 185 SVVVSFGP 208
S +VS GP
Sbjct: 79737 SKIVSLGP 79714
Score = 37.7 bits (76), Expect(2) = 4e-021
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3 / -1
Query: 195 *VLVPRENVKNTDANWSCIS 254
*VLVPRENVKNT A+ +CIS
Sbjct: 79727 *VLVPRENVKNTGASCNCIS 79668
>gb|AC152887.20| Medicago truncatula clone mth2-91j4, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 116277
Score = 171 bits (369), Expect = 2e-042
Identities = 64/83 (77%), Positives = 78/83 (93%)
Frame = -1 / -1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
++MQLQLASVFFTF LGTK HYYG TLLHGG++YR TGRGFVVFHAKFA+NYR+YSRSHF
Sbjct: 97275 IIMQLQLASVFFTFQLGTKAHYYGRTLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHF 97096
Query: 76 VKGIELMILLIVYEIFGQSYRGA 8
VKG+E++ILLI+YE++G+SYR +
Sbjct: 97095 VKGLEILILLIIYEVYGESYRSS 97027
>gb|AC157648.18| Medicago truncatula clone mth2-68d9, complete sequence
Length = 84361
Score = 171 bits (369), Expect = 2e-042
Identities = 64/83 (77%), Positives = 78/83 (93%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
++MQLQLASVFFTF LGTK HYYG TLLHGG++YR TGRGFVVFHAKFA+NYR+YSRSHF
Sbjct: 76559 IIMQLQLASVFFTFQLGTKAHYYGRTLLHGGSKYRPTGRGFVVFHAKFADNYRMYSRSHF 76738
Query: 76 VKGIELMILLIVYEIFGQSYRGA 8
VKG+E++ILLI+YE++G+SYR +
Sbjct: 76739 VKGLEILILLIIYEVYGESYRSS 76807
>gb|AC158209.13| Medicago truncatula clone mth2-154h5, complete sequence
Length = 93864
Score = 152 bits (327), Expect = 1e-036
Identities = 61/77 (79%), Positives = 69/77 (89%)
Frame = -1 / -2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
++MQLQLA VFFTFSLGTK HY+G TLLHGGA+YRATGRGFVV H KFA+NYR+YSRSHF
Sbjct: 55949 IIMQLQLAPVFFTFSLGTKLHYFGRTLLHGGAKYRATGRGFVVRHEKFADNYRMYSRSHF 55770
Query: 76 VKGIELMILLIVYEIFG 26
VKGIEL +LLI Y I+G
Sbjct: 55769 VKGIELALLLICYMIYG 55719
>gb|BF641357.1|BF641357 NF061B11IN1F1092 Insect herbivory Medicago truncatula cDNA clone
NF061B11IN 5', mRNA sequence
Length = 660
Score = 140 bits (300), Expect = 6e-033
Identities = 56/71 (78%), Positives = 64/71 (90%)
Frame = -1 / +1
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVK 71
MQLQL S+FFTFSLGT+TH++G T+LHGGA+YRATGRGFVV H FAENYRLY+RSHFVK
Sbjct: 391 MQLQLGSLFFTFSLGTRTHFFGRTILHGGAKYRATGRGFVVEHKSFAENYRLYARSHFVK 570
Query: 70 GIELMILLIVY 38
IEL I+LIVY
Sbjct: 571 AIELGIILIVY 603
>gb|AC150782.12| Medicago truncatula clone mth2-116a21, WORKING DRAFT SEQUENCE, 3
unordered pieces
Length = 118726
Score = 131 bits (281), Expect = 2e-030
Identities = 50/71 (70%), Positives = 64/71 (90%)
Frame = -1 / +3
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVK 71
MQLQL+SVF+TFS+GT++H++G T+LHGGA+YRATGRGFVV H FAE YRL+SRSHFVK
Sbjct: 21243 MQLQLSSVFYTFSMGTRSHFFGRTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVK 21422
Query: 70 GIELMILLIVY 38
IEL ++L++Y
Sbjct: 21423 AIELGLILVIY 21455
>gb|AC158736.12| Medicago truncatula clone mth2-165n2, WORKING DRAFT SEQUENCE
Length = 118519
Score = 131 bits (281), Expect = 2e-030
Identities = 50/71 (70%), Positives = 64/71 (90%)
Frame = -1 / -1
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVK 71
MQLQL+SVF+TFS+GT++H++G T+LHGGA+YRATGRGFVV H FAE YRL+SRSHFVK
Sbjct: 11554 MQLQLSSVFYTFSMGTRSHFFGRTILHGGAKYRATGRGFVVEHKSFAEIYRLFSRSHFVK 11375
Query: 70 GIELMILLIVY 38
IEL ++L++Y
Sbjct: 11374 AIELGLILVIY 11342
>gb|BG449853.1|BG449853 NF053H03IN1F1029 Insect herbivory Medicago truncatula cDNA clone
NF053H03IN 5', mRNA sequence
Length = 646
Score = 128 bits (275), Expect = 2e-029
Identities = 50/60 (83%), Positives = 56/60 (93%)
Frame = -1 / +3
Query: 181 TLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYEIFGQSYRGAIA 2
TLLHGGA+YR TGRGFVVFHAKFA+NYRLYSRSHFVKGIEL+ILL+VY IFG +YRG +A
Sbjct: 6 TLLHGGAQYRGTGRGFVVFHAKFADNYRLYSRSHFVKGIELVILLVVYHIFGHAYRGVVA 185
>gb|BF004482.1|BF004482 EST432980 KV1 Medicago truncatula cDNA clone pKV1-17D12, mRNA
sequence
Length = 617
Score = 119 bits (254), Expect = 1e-026
Identities = 47/54 (87%), Positives = 51/54 (94%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRL 95
+LMQLQLA VFFTFSLGTKTHY+G TLLHGGA+YR TGRGFVVFHAKFA+NYRL
Sbjct: 455 ILMQLQLAPVFFTFSLGTKTHYFGRTLLHGGAKYRPTGRGFVVFHAKFADNYRL 616
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 269,896,886
Number of Sequences: 392609
Number of extensions: 3130177
Number of successful extensions: 130520
Number of sequences better than 1.0e-020: 15
length of database: 147,244,331
effective HSP length: 50
effective length of database: 127,613,881
effective search space used: 5232169121
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)