TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH17d04.yg.2.1
(637 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY557602.1| Medicago truncatula cultivar Jemalong core a... 139 7e-057
gb|AC175027.3| Medicago truncatula clone mth2-102d9, WORKIN... 137 2e-056
emb|AJ416755.1|MTR416755 Medicago truncatula partial mRNA f... 201 7e-051
>gb|AY557602.1| Medicago truncatula cultivar Jemalong core alpha
1,3-fucosyltransferase (FUT2) gene, complete cds
Length = 3124
Score = 139 bits (298), Expect(3) = 7e-057
Identities = 50/76 (65%), Positives = 63/76 (82%)
Frame = -1 / +1
Query: 400 RWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYH 221
RWKY+GPSDSFKAL+DMAAVHSSCRLCIH+A+K KEE++P F RPC C+ T+YH
Sbjct: 2185 RWKYEGPSDSFKALVDMAAVHSSCRLCIHLASKSREKEEKSPDFKRRPCKCTRGSETVYH 2364
Query: 220 LFIRERGRFKSESIYM 173
+++RERG F+ ESIY+
Sbjct: 2365 IYVRERGTFEMESIYL 2412
Score = 123 bits (264), Expect(2) = 4e-030
Identities = 51/99 (51%), Positives = 68/99 (68%)
Frame = -1 / +1
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVAS 458
RY+FS FE E DY EKFFQ V G+IPVVVG PNIQ+F P G+ L+I L+DV S
Sbjct: 1696 RYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGPPNIQDFAPSPGSFLYIKELEDVES 1875
Query: 457 VAKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAV 341
VAK+M+ +A NP+A+NQSLR Y ++ F L+ ++ +
Sbjct: 1876 VAKSMRYLAENPEAYNQSLR*NYMMKTNKFLRLLFLSCI 1992
Score = 95.9 bits (203), Expect(3) = 7e-057
Identities = 38/56 (67%), Positives = 46/56 (82%)
Frame = -1 / +2
Query: 169 SGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
S LTL + ++AVL KF SLNHVPVWK ERP ++GGD+LK+Y+IYP GLTQRQAL
Sbjct: 2870 SSNLTLESFKTAVLTKFTSLNHVPVWKPERPEILKGGDELKVYKIYPAGLTQRQAL 3037
Score = 92.3 bits (195), Expect(3) = 1e-038
Identities = 46/81 (56%), Positives = 52/81 (64%)
Frame = +3 / -3
Query: 393 FHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXTXX* 572
F+L D*L ASGF A + + LAT++TSSS L+ P G S*IFG PTTTGI P T
Sbjct: 1940 FYLND*LYASGFSARYLMDLATDSTSSSSLMYKNEPGEGAKS*IFGGPTTTGIVPATSDW 1761
Query: 573 KNFSXX*SXSXXXSNXXLNL* 635
KNFS *S S SN LNL*
Sbjct: 1760 KNFSVT*SSSLEFSNAKLNL* 1698
Score = 73.9 bits (155), Expect(3) = 1e-038
Identities = 33/50 (66%), Positives = 38/50 (76%)
Frame = +3 / -3
Query: 252 LQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHL 401
LQGR + G+ SSF + A +QRRQDE TAA+S RALKESDGPSYFHL
Sbjct: 2333 LQGRLLKSGLFSSFSLLFEAK*MQRRQDECTAAISTRALKESDGPSYFHL 2184
Score = 40.0 bits (81), Expect(3) = 1e-038
Identities = 21/52 (40%), Positives = 23/52 (44%)
Frame = +3 / -2
Query: 15 CVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPD 170
CV+P G IL F FHTGT RD+N TA K RV D
Sbjct: 3024 CVKPAGYIL*TFNSSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLD 2869
Score = 32.7 bits (65), Expect(2) = 4e-030
Identities = 19/43 (44%), Positives = 24/43 (55%)
Frame = -3 / +1
Query: 158 NSGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEIIQNLP 30
NSG L + + VP+ SCME *K RW * E +QN+P
Sbjct: 2881 NSGVLQDCCSYEVHVPESCSCMEA*KT*NSKRW**IEGLQNIP 3009
Score = 32.2 bits (64), Expect(3) = 7e-057
Identities = 21/43 (48%), Positives = 21/43 (48%)
Frame = -3 / +3
Query: 635 LQIQXXI*XXX*XGLXX*KVFSVXSXRFYSGCCWCSKYSRVXS 507
LQI I* GL *KVF V Y CW SKYSR S
Sbjct: 1698 LQI*LSI*KF*RGGLCN*KVFPVACCWNYPCGCWSSKYSRFCS 1826
>gb|AC175027.3| Medicago truncatula clone mth2-102d9, WORKING DRAFT SEQUENCE, 16
unordered pieces
Length = 124356
Score = 137 bits (293), Expect(3) = 2e-056
Identities = 50/77 (64%), Positives = 63/77 (81%)
Frame = -1 / -1
Query: 400 RWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYH 221
RWKY+GPSDSFKAL+DMAAVHSSCRLCIH+A+K KEE++P F R C C+ T+YH
Sbjct: 93012 RWKYEGPSDSFKALVDMAAVHSSCRLCIHLASKSREKEEKSPDFKKRLCKCTRGSETVYH 92833
Query: 220 LFIRERGRFKSESIYMR 170
+++RERG F+ ESIY+R
Sbjct: 92832 IYVRERGTFEMESIYLR 92782
Score = 98.2 bits (208), Expect(3) = 2e-056
Identities = 39/57 (68%), Positives = 47/57 (82%)
Frame = -1 / -2
Query: 172 RSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
RS LTL + ++AVL KF SLNHVPVWK ERP ++GGD+LK+Y+IYP GLTQRQAL
Sbjct: 92330 RSSNLTLESFKTAVLTKFTSLNHVPVWKPERPEILKGGDELKVYKIYPAGLTQRQAL 92160
Score = 73.9 bits (155), Expect(4) = 3e-031
Identities = 33/50 (66%), Positives = 38/50 (76%)
Frame = +3 / +2
Query: 252 LQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHL 401
LQ RF+ G+ SSF + A +QRRQDE TAA+S RALKESDGPSYFHL
Sbjct: 92864 LQSRFLKSGLFSSFSLLFEAK*MQRRQDECTAAISTRALKESDGPSYFHL 93013
Score = 71.6 bits (150), Expect(4) = 3e-031
Identities = 33/57 (57%), Positives = 39/57 (68%)
Frame = +3 / +2
Query: 393 FHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXT 563
F+L D*L ASGF A + + LAT++TSSS L+ P G S*IFG PTTTGI P T
Sbjct: 93257 FYLND*LYASGFSARYLMDLATDSTSSSSLMYKNEPGEGAKS*IFGGPTTTGIVPTT 93427
Score = 41.8 bits (85), Expect(4) = 3e-031
Identities = 22/53 (41%), Positives = 24/53 (45%)
Frame = +3 / +1
Query: 15 CVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDL 173
CV+P G IL F FHTGT RD+N TA K RV DL
Sbjct: 92173 CVKPAGYIL*TFNSSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDL 92331
Score = 41.4 bits (84), Expect(3) = 2e-056
Identities = 16/28 (57%), Positives = 17/28 (60%)
Frame = -1 / -1
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXG 554
RY+FS FE E DY EKFFQ V G
Sbjct: 94272 RYKFSLAFENSNEEDYVTEKFFQSLVAG 94189
Score = 29.0 bits (57), Expect(4) = 3e-031
Identities = 15/27 (55%), Positives = 15/27 (55%)
Frame = +3 / +2
Query: 555 PXTXX*KNFSXX*SXSXXXSNXXLNL* 635
P T KNFS *S S SN LNL*
Sbjct: 94190 PATSDWKNFSVT*SSSLEFSNAKLNL* 94270
>emb|AJ416755.1|MTR416755 Medicago truncatula partial mRNA for alpha1,3-fucosyltransferase
(fuct3 gene)
Length = 658
Score = 201 bits (434), Expect = 7e-051
Identities = 79/121 (65%), Positives = 96/121 (79%)
Frame = -1 / +2
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVAS 458
RY+FS FE E DY EKFFQ V G+IPVVVG PNIQ+F P G+ L+I L+DV S
Sbjct: 293 RYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGPPNIQDFAPSPGSFLYIKELEDVES 472
Query: 457 VAKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERT 278
VAK+M+ +A NP+A+NQSLRWKY+GPSDSFKAL+DMA VHSSCRLCIH+A+K KEE++
Sbjct: 473 VAKSMRYLAENPEAYNQSLRWKYEGPSDSFKALVDMAXVHSSCRLCIHLASKSREKEEKS 652
Query: 277 P 275
P
Sbjct: 653 P 655
Score = 147 bits (315), Expect = 2e-034
Identities = 72/120 (60%), Positives = 81/120 (67%)
Frame = +3 / -2
Query: 276 GVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHLKD*LKASGFEAIFFIVLA 455
G+ SSF + A +QRRQDE T A+S RALKESDGPSYFHL D*L ASGF A + + LA
Sbjct: 654 GLFSSFSLLFEAK*MQRRQDECTXAISTRALKESDGPSYFHLND*LYASGFSARYLMDLA 475
Query: 456 TEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXTXX*KNFSXX*SXSXXXSNXXLNL* 635
T++TSSS L+ P G S*IFG PTTTGI P T KNFS *S S SN LNL*
Sbjct: 474 TDSTSSSSLMYKNEPGEGAKS*IFGGPTTTGIVPATSDWKNFSVT*SSSLEFSNAKLNL* 295
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 580,074,804
Number of Sequences: 392609
Number of extensions: 7796433
Number of successful extensions: 34535
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 19788820865
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)