TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN21e06.xg.2.1
(704 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG448529.1|BG448529 NF036G02RT1F1017 Developing root Med... 193 2e-053
gb|BG450908.1|BG450908 NF095E08DT1F1065 Drought Medicago tr... 183 9e-052
gb|AC166046.6| Medicago truncatula clone mth2-6p7, WORKING ... 88 9e-043
gb|AL382396.1|AL382396 MtBC06E08R1 MtBC Medicago truncatula... 166 9e-042
gb|AC138171.17| Medicago truncatula clone mth2-7f10, comple... 98 4e-040
gb|AC170856.1| Medicago truncatula chromosome 2 clone mth2-... 89 5e-040
gb|AC147428.6| Medicago truncatula clone mth2-34i10, comple... 84 2e-039
gb|AL375055.1|AL375055 MtBB11A08R1 MtBB Medicago truncatula... 149 4e-035
gb|AL375054.1|AL375054 MtBB11A08F1 MtBB Medicago truncatula... 82 9e-032
gb|AL382395.1|AL382395 MtBC06E08F1 MtBC Medicago truncatula... 87 1e-030
gb|AW690621.2|AW690621 NF036F01ST1F1000 Developing stem Med... 122 6e-027
>gb|BG448529.1|BG448529 NF036G02RT1F1017 Developing root Medicago truncatula cDNA clone
NF036G02RT 5', mRNA sequence
Length = 468
Score = 193 bits (417), Expect(2) = 2e-053
Identities = 75/119 (63%), Positives = 93/119 (78%)
Frame = +3 / +1
Query: 129 ESLLTMFWDHQDAVLCCSFKEKPVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKAL 308
ES+L W H DAVLCC+ K PVFTF NQ G+DMLETTLVALQD++L+KI D+ GRK L
Sbjct: 91 ESVLKSLWHHSDAVLCCTLKAMPVFTFSNQAGLDMLETTLVALQDISLEKIFDDNGRKTL 270
Query: 309 HAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
+E P++++QG+A L G+CLS MGR VSYE+AVAWKVL ++ N HC+ F FVNW FV*
Sbjct: 271 FSEFPQIIQQGFACLQGGICLSSMGRPVSYERAVAWKVLNDEQNAHCICFMFVNWXFV* 447
Score = 122 bits (260), Expect(2) = 4e-028
Identities = 60/119 (50%), Positives = 71/119 (59%)
Frame = -2 / -2
Query: 484 QTKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSACS 305
QTK Q T K Q SS TFQATA S ET RP+ +R P R+A PC I +G S
Sbjct: 446 QTKXQFTNIKQIQ*AFCSSFSTFQATALSYETGRPMLERQIPPCRHAKPC*IIWGNSEKR 267
Query: 304 AFLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTGFSLKEQHKTAS*WSQNIVKSDS 128
F P SS I SR+ SC AT VVS+MS+P W KVNTG + + QH TAS W + +DS
Sbjct: 266 VFRPLSSNIFSRLISCNATSVVSSMSSPAWFEKVNTGIAFRVQHNTASEWCHKDLSTDS 90
Score = 36.8 bits (74), Expect(2) = 2e-053
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +3 / +3
Query: 42 EAATLVRWICQSYRYHLGADLV 107
EA TL WIC SYR +LG +L+
Sbjct: 3 EAQTLAHWICNSYRCYLGVELL 68
Score = 24.0 bits (46), Expect(2) = 4e-028
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = -2 / -3
Query: 109 LTKSAPK*YR*LWQIQRTSVAAS 41
L+ S PK*+ *L QIQ V AS
Sbjct: 70 LSSSTPK*HL*LLQIQ*ARVCAS 2
>gb|BG450908.1|BG450908 NF095E08DT1F1065 Drought Medicago truncatula cDNA clone NF095E08DT
5', mRNA sequence
Length = 694
Score = 183 bits (394), Expect(2) = 9e-052
Identities = 71/119 (59%), Positives = 90/119 (75%)
Frame = +3 / -1
Query: 129 ESLLTMFWDHQDAVLCCSFKEKPVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKAL 308
ES+L H DAV CCS K PVFTF NQ G+DMLETTLVALQD+TL+KI D+ G+K L
Sbjct: 571 ESILKSLXHHSDAVXCCSLKALPVFTFANQAGLDMLETTLVALQDITLEKIFDDNGKKTL 392
Query: 309 HAEVPKLMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
E P++M+QG+ + G+CLS MGR ++YE+AVAWKVL E+ + HC+ F F+NWSFV*
Sbjct: 391 CXEFPQIMQQGFMCIQGGICLSSMGRPITYERAVAWKVLNEEESAHCICFMFINWSFV* 215
Score = 41.8 bits (85), Expect(2) = 9e-052
Identities = 16/28 (57%), Positives = 21/28 (75%)
Frame = +3 / -1
Query: 24 LISGSPEAATLVRWICQSYRYHLGADLV 107
L G+ EA TL RWIC S R++LGA+L+
Sbjct: 670 LPPGTXEAQTLTRWICNSXRFYLGAELL 587
>gb|AC166046.6| Medicago truncatula clone mth2-6p7, WORKING DRAFT SEQUENCE, 8
unordered pieces
Length = 118925
Score = 87.7 bits (185), Expect(4) = 9e-043
Identities = 33/49 (67%), Positives = 39/49 (79%)
Frame = +3 / +2
Query: 339 GYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
G+A L G+CLS MGR VSYE+AVAWKVL ++ N HC+ F FVNWSFV*
Sbjct: 9425 GFACLQGGICLSSMGRPVSYERAVAWKVLNDEQNAHCICFMFVNWSFV* 9571
Score = 83.1 bits (175), Expect(4) = 9e-043
Identities = 33/53 (62%), Positives = 44/53 (83%)
Frame = +3 / +2
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYL 353
PVFTF NQ G+DMLETTLVALQD++L+KI D+ GRK L +E P++++Q Y +L
Sbjct: 9125 PVFTFSNQAGLDMLETTLVALQDISLEKIFDDNGRKTLFSEFPQIIQQVYNFL 9283
Score = 45.5 bits (93), Expect(4) = 9e-043
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +3 / +3
Query: 81 RYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFK 188
R +LG +L+ + + ES+L W H DAVLCC+ K
Sbjct: 8622 RCYLGVELLKSNTEGKESVLKSLWHHSDAVLCCTLK 8729
Score = 40.0 bits (81), Expect(4) = 9e-043
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +3 / +3
Query: 12 AGQKLISGSPEAATLVRWICQSYRY 86
AG + G+PEA TL WIC SYRY
Sbjct: 7905 AGLRSPLGTPEAQTLAHWICNSYRY 7979
>gb|AL382396.1|AL382396 MtBC06E08R1 MtBC Medicago truncatula cDNA clone MtBC06E08 T7, mRNA
sequence
Length = 526
Score = 166 bits (357), Expect(2) = 9e-042
Identities = 66/97 (68%), Positives = 78/97 (80%)
Frame = +3 / +1
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLS 374
P+FTF NQ G+DMLETTLVALQD+ LDK+ DE GRK L +E K+M+QGYA LP G+C+S
Sbjct: 46 PIFTFSNQAGLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQGYASLPGGICVS 225
Query: 375 GMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
M R VSYEQA+AWKVL +D HCLAF F+NWSFV*
Sbjct: 226 SMNRPVSYEQAIAWKVLNDDDANHCLAFMFINWSFV* 336
Score = 106 bits (227), Expect = 3e-022
Identities = 53/98 (54%), Positives = 60/98 (61%)
Frame = -2 / -3
Query: 487 HQTKDQLTKQKARQCTLPSSPRTFQATACS*ETWRPIPDRHTPAGRYA*PCSISFGTSAC 308
HQTKDQL KA+Q SS RTF A ACS*+T + D P GR A*PC I S
Sbjct: 338 HQTKDQLMNMKAKQ*LASSSLRTFHAMACS*DTGLFMLDTQIPPGRDA*PCCIILENSEQ 159
Query: 307 SAFLPGSSEILSRVRSCRATKVVSNMSTPIWLPKVNTG 194
FLP SS LS + S RAT+VVS+MS+P W KVN G
Sbjct: 158 RIFLPASSRTLSSIIS*RATRVVSSMSSPAWFEKVNIG 45
Score = 25.4 bits (49), Expect(2) = 9e-042
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +3 / +1
Query: 156 HQDAVLCCSFK 188
H DA++CCS K
Sbjct: 1 HSDAIMCCSVK 33
>gb|AC138171.17| Medicago truncatula clone mth2-7f10, complete sequence
Length = 104336
Score = 98.2 bits (208), Expect(3) = 4e-040
Identities = 35/50 (70%), Positives = 43/50 (86%)
Frame = +3 / +2
Query: 336 QGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
QG+A +PAG+C+S MGRH SY+QAVAWKV ED +VHCLAF F+NWSF+*
Sbjct: 82934 QGFACMPAGICMSTMGRHASYDQAVAWKVHAEDNSVHCLAFSFINWSFI* 83083
Score = 79.4 bits (167), Expect(3) = 4e-040
Identities = 35/55 (63%), Positives = 40/55 (72%)
Frame = +3 / +2
Query: 174 CCSFKEKPVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQ 338
C ++ PVF F NQ G+DMLETTLVALQD+TLDKI DE RK L A KLM+Q
Sbjct: 82643 CLVLQQVPVFIFANQAGLDMLETTLVALQDITLDKIFDESARKNLIAYFAKLMQQ 82807
Score = 44.1 bits (90), Expect(3) = 4e-040
Identities = 18/33 (54%), Positives = 22/33 (66%)
Frame = +3 / +2
Query: 9 TAGQKLISGSPEAATLVRWICQSYRYHLGADLV 107
+ G KL G+PEA TL +WICQSYR L L+
Sbjct: 82277 SGGAKLSPGTPEALTLAQWICQSYR*SLHESLM 82375
>gb|AC170856.1| Medicago truncatula chromosome 2 clone mth2-13d14, *** SEQUENCING IN
PROGRESS ***, 2 ordered pieces
Length = 101063
Score = 89.0 bits (188), Expect(4) = 5e-040
Identities = 34/53 (64%), Positives = 41/53 (77%)
Frame = +3 / -3
Query: 327 LMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
L+ QG+A L G+CLS MGR +SYE+ VAWKVL E+ N HC+ F FVNWSFV*
Sbjct: 70578 LIWQGFACLQGGLCLSSMGRPISYERVVAWKVLNEEENAHCICFMFVNWSFV* 70420
Score = 75.8 bits (159), Expect(4) = 5e-040
Identities = 31/48 (64%), Positives = 39/48 (81%)
Frame = +3 / -1
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQ 338
PVFTF NQ G+DMLETTLVALQD+ L+KI D+ GRK L +E P +++Q
Sbjct: 70883 PVFTFANQAGLDMLETTLVALQDIALEKIFDDHGRKILCSEFPLIIQQ 70740
Score = 48.3 bits (99), Expect(4) = 5e-040
Identities = 17/36 (47%), Positives = 24/36 (66%)
Frame = +3 / -1
Query: 81 RYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFK 188
R LGA+L+ +++ ESLL W H DA+LCC+ K
Sbjct: 71327 RCFLGAELLKSNNEGSESLLKSLWHHTDAILCCTLK 71220
Score = 33.1 bits (66), Expect(4) = 5e-040
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +3 / -3
Query: 33 GSPEAATLVRWICQSYR 83
G+PEA TL RWI SYR
Sbjct: 71511 GTPEAQTLARWISNSYR 71461
>gb|AC147428.6| Medicago truncatula clone mth2-34i10, complete sequence
Length = 112873
Score = 84.5 bits (178), Expect(4) = 2e-039
Identities = 29/53 (54%), Positives = 41/53 (77%)
Frame = +3 / +2
Query: 327 LMEQGYAYLPAGVCLSGMGRHVSYEQAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
++ QG+ + G+CLS MGR ++YE+AVAWKVL E+ + HC+ F F+NWSFV*
Sbjct: 45218 ILVQGFMCIQGGICLSSMGRPITYERAVAWKVLNEEESAHCICFMFINWSFV* 45376
Score = 80.3 bits (169), Expect(4) = 2e-039
Identities = 32/48 (66%), Positives = 41/48 (85%)
Frame = +3 / +2
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQ 338
PVFTF NQ G+DMLETTLVALQD+TL+KI D+ G+K L +E P++M+Q
Sbjct: 44906 PVFTFANQAGLDMLETTLVALQDITLEKIFDDNGKKTLCSEFPQIMQQ 45049
Score = 40.9 bits (83), Expect(4) = 2e-039
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = +3 / +1
Query: 33 GSPEAATLVRWICQSYRY 86
G+PEA TL RWIC SYRY
Sbjct: 44488 GTPEAQTLTRWICNSYRY 44541
Score = 38.6 bits (78), Expect(4) = 2e-039
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +3 / +1
Query: 120 QAGESLLTMFWDHQDAVLCCSFK 188
+ ES+L W H DAVLCCS K
Sbjct: 44677 EGSESILKSLWHHSDAVLCCSLK 44745
>gb|AL375055.1|AL375055 MtBB11A08R1 MtBB Medicago truncatula cDNA clone MtBB11A08 T7, mRNA
sequence
Length = 535
Score = 149 bits (320), Expect = 4e-035
Identities = 60/88 (68%), Positives = 71/88 (80%)
Frame = +3 / +1
Query: 222 GVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYAYLPAGVCLSGMGRHVSYE 401
G+DMLETTLVALQD+ LDK+ DE GRK L +E K+M+QGYA LP G+C+S M R VSYE
Sbjct: 1 GLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQGYASLPGGICVSSMNRPVSYE 180
Query: 402 QAVAWKVLGEDGNVHCLAFCFVNWSFV* 485
QA+AWKVL +D HCLAF F+NWSFV*
Sbjct: 181 QAIAWKVLNDDDANHCLAFMFINWSFV* 264
>gb|AL375054.1|AL375054 MtBB11A08F1 MtBB Medicago truncatula cDNA clone MtBB11A08 T3, mRNA
sequence
Length = 492
Score = 82.2 bits (173), Expect(2) = 9e-032
Identities = 33/49 (67%), Positives = 40/49 (81%)
Frame = +3 / +1
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQG 341
P+FTF NQ G+DMLETTLVALQD+ LDK+ DE GRK L +E K+M+QG
Sbjct: 346 PIFTFSNQAGLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQG 492
Score = 76.2 bits (160), Expect(2) = 9e-032
Identities = 28/58 (48%), Positives = 36/58 (62%)
Frame = +3 / +1
Query: 15 GQKLISGSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFK 188
G K + GSPEA L RWI +SYR H GA+L A +++L W H DA++CCS K
Sbjct: 160 GPKSLPGSPEAHALARWISRSYRMHTGAELFRVESAASDAILKQLWHHSDAIMCCSVK 333
>gb|AL382395.1|AL382395 MtBC06E08F1 MtBC Medicago truncatula cDNA clone MtBC06E08 T3, mRNA
sequence
Length = 481
Score = 86.8 bits (183), Expect(2) = 1e-030
Identities = 35/51 (68%), Positives = 42/51 (82%)
Frame = +3 / +3
Query: 195 PVFTFGNQMGVDMLETTLVALQDLTLDKISDEPGRKALHAEVPKLMEQGYA 347
P+FTF NQ G+DMLETTLVALQD+ LDK+ DE GRK L +E K+M+QGYA
Sbjct: 324 PIFTFSNQAGLDMLETTLVALQDIMLDKVLDEXGRKILCSEFSKIMQQGYA 476
Score = 68.0 bits (142), Expect(2) = 1e-030
Identities = 25/58 (43%), Positives = 32/58 (55%)
Frame = +3 / +3
Query: 15 GQKLISGSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFK 188
G K + GSPEA L RWI + YR H GA L +++L W H A++CCS K
Sbjct: 138 GPKSLPGSPEAHALARWISRXYRMHTGAXLFRVESAXSDAILKQLWHHSXAIMCCSVK 311
>gb|AW690621.2|AW690621 NF036F01ST1F1000 Developing stem Medicago truncatula cDNA clone
NF036F01ST 5', mRNA sequence
Length = 425
Score = 122 bits (261), Expect = 6e-027
Identities = 48/78 (61%), Positives = 58/78 (74%)
Frame = +3 / +1
Query: 33 GSPEAATLVRWICQSYRYHLGADLVSHSDQAGESLLTMFWDHQDAVLCCSFKEKPVFTFG 212
G+PEA TL RWI SYR LGA+L+ +++ ESLL W H DA+LCC+ K PVFTF
Sbjct: 175 GTPEAQTLARWISNSYRCFLGAELLKSNNEGSESLLKSLWHHTDAILCCTLKALPVFTFA 354
Query: 213 NQMGVDMLETTLVALQDL 266
NQ G+DMLETTLVALQD+
Sbjct: 355 NQAGLDMLETTLVALQDI 408
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 681,467,507
Number of Sequences: 392609
Number of extensions: 9269741
Number of successful extensions: 40127
Number of sequences better than 1.0e-020: 16
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22687820100
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)