TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.4
(664 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG449351.1|BG449351 NF046F07IN1F1061 Insect herbivory Me... 98 4e-035
gb|AL371111.1|AL371111 MtBA42A01F1 MtBA Medicago truncatula... 98 4e-032
gb|BI271829.1|BI271829 NF012D11FL1F1093 Developing flower M... 98 9e-027
gb|AC142396.25| Medicago truncatula clone mth2-6g9, complet... 69 1e-026
gb|AA660590.1|AA660590 00476 MtRHE Medicago truncatula cDNA... 64 3e-026
gb|BQ164948.1|BQ164948 EST610817 KVKC Medicago truncatula c... 98 6e-026
gb|CB891199.1|CB891199 EST648168 KV3 Medicago truncatula cD... 66 1e-025
gb|BQ164947.1|BQ164947 EST610816 KVKC Medicago truncatula c... 62 2e-024
>gb|BG449351.1|BG449351 NF046F07IN1F1061 Insect herbivory Medicago truncatula cDNA clone
NF046F07IN 5', mRNA sequence
Length = 670
Score = 97.7 bits (207), Expect(3) = 4e-035
Identities = 37/51 (72%), Positives = 41/51 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA EWC+TD FDPNN +H KAEIE+GDGLPDIR
Sbjct: 247 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIR 399
Score = 61.6 bits (128), Expect(3) = 4e-035
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = -2 / +1
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEA 247
G+ + C +K DFM MP DN+FDA YAI+ATC+AP+A
Sbjct: 130 GVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDA 243
Score = 28.1 bits (55), Expect(3) = 4e-035
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL N+Y ++RGK
Sbjct: 55 SSTSVTGLNNNEYQITRGK 111
>gb|AL371111.1|AL371111 MtBA42A01F1 MtBA Medicago truncatula cDNA clone MtBA42A01 T3, mRNA
sequence
Length = 489
Score = 97.7 bits (207), Expect(2) = 4e-032
Identities = 37/51 (72%), Positives = 41/51 (80%)
Frame = -2 / +3
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA EWC+TD FDPNN +H KAEIE+GDGLPDIR
Sbjct: 123 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIR 275
Score = 61.6 bits (128), Expect(2) = 4e-032
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = -2 / +3
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEA 247
G+ + C +K DFM MP DN+FDA YAI+ATC+AP+A
Sbjct: 6 GVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDA 119
>gb|BI271829.1|BI271829 NF012D11FL1F1093 Developing flower Medicago truncatula cDNA clone
NF012D11FL 5', mRNA sequence
Length = 675
Score = 97.7 bits (207), Expect(2) = 9e-027
Identities = 37/51 (72%), Positives = 41/51 (80%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA EWC+TD FDPNN +H KAEIE+GDGLPDIR
Sbjct: 71 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIR 223
Score = 43.7 bits (89), Expect(2) = 9e-027
Identities = 15/22 (68%), Positives = 19/22 (86%)
Frame = -2 / +2
Query: 312 PIADNTFDAAYAIQATCYAPEA 247
P DN+FDA YAI+ATC+AP+A
Sbjct: 2 PFEDNSFDAVYAIEATCHAPDA 67
>gb|AC142396.25| Medicago truncatula clone mth2-6g9, complete sequence
Length = 106058
Score = 68.9 bits (144), Expect(4) = 1e-026
Identities = 26/46 (56%), Positives = 32/46 (69%)
Frame = -2 / +3
Query: 177 LYPWLSQQGVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTK 40
L+ + + G Y E+YRVLKPGQ FA EWC+TD FDPNN +H K
Sbjct: 56973 LFLYYA*YGCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIK 57110
Score = 61.1 bits (127), Expect(4) = 1e-026
Identities = 21/32 (65%), Positives = 27/32 (84%)
Frame = -2 / +3
Query: 336 LKGDFMNMPIADNTFDAAYAIQATCYAPEAVC 241
++ DFM MP DN+FDA YAI+ATC+AP+AVC
Sbjct: 56676 MQADFMKMPFEDNSFDAVYAIEATCHAPDAVC 56771
Score = 37.3 bits (75), Expect(4) = 1e-026
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = -2 / +3
Query: 513 SSTXXXGLXXNDYHVSRGKVCIL 445
SST GL N+Y ++RGKVC+L
Sbjct: 55404 SSTSVTGLNNNEYQITRGKVCVL 55472
Score = 32.7 bits (65), Expect(4) = 1e-026
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = -2 / +1
Query: 42 KAEIELGDGLPDIR 1
+AEIE+GDGLPDIR
Sbjct: 57193 QAEIEIGDGLPDIR 57234
>gb|AA660590.1|AA660590 00476 MtRHE Medicago truncatula cDNA 5' similar to
delta24-sterol-methyltransferase, mRNA sequence
Length = 555
Score = 64.3 bits (134), Expect(4) = 3e-026
Identities = 23/31 (74%), Positives = 25/31 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNN 61
G Y E+YRVLKPGQ FA EWC+TD FDPNN
Sbjct: 271 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPNN 363
Score = 61.6 bits (128), Expect(4) = 3e-026
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = -2 / +1
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEA 247
G+ + C +K DFM MP DN+FDA YAI+ATC+AP+A
Sbjct: 154 GVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDA 267
Score = 25.4 bits (49), Expect(4) = 3e-026
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL N+Y ++ GK
Sbjct: 79 SSTSVTGLNNNEYQITXGK 135
Score = 23.5 bits (45), Expect(4) = 3e-026
Identities = 10/19 (52%), Positives = 11/19 (57%)
Frame = -2 / +2
Query: 78 LFDPNNAKHLTTKAEIELG 22
L P KH KAEIE+G
Sbjct: 347 LLIPITKKHQXIKAEIEIG 403
>gb|BQ164948.1|BQ164948 EST610817 KVKC Medicago truncatula cDNA clone pKVKC-3H9, mRNA
sequence
Length = 778
Score = 97.7 bits (207), Expect(2) = 6e-026
Identities = 37/51 (72%), Positives = 41/51 (80%)
Frame = -2 / -1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKHLTTKAEIELGDGLPDIR 1
G Y E+YRVLKPGQ FA EWC+TD FDPNN +H KAEIE+GDGLPDIR
Sbjct: 712 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPNNQEHQKIKAEIEIGDGLPDIR 560
Score = 40.9 bits (83), Expect(2) = 6e-026
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = -2 / -1
Query: 303 DNTFDAAYAIQATCYAPEA 247
DN+FDA YAI+ATC+AP+A
Sbjct: 772 DNSFDAVYAIEATCHAPDA 716
>gb|CB891199.1|CB891199 EST648168 KV3 Medicago truncatula cDNA clone KV3-49B3, mRNA
sequence
Length = 624
Score = 65.7 bits (137), Expect(3) = 1e-025
Identities = 23/34 (67%), Positives = 27/34 (79%)
Frame = -2 / +2
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPNNAKH 52
G Y E+YRVLKPGQ FA EWC+TD FDP+N +H
Sbjct: 521 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPHNPEH 622
Score = 61.6 bits (128), Expect(3) = 1e-025
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = -2 / +2
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEA 247
G+ + C +K DFM MP DN+FDA YAI+ATC+AP+A
Sbjct: 404 GVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDA 517
Score = 28.1 bits (55), Expect(3) = 1e-025
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2 / +2
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL N+Y ++RGK
Sbjct: 329 SSTSVTGLNNNEYQITRGK 385
>gb|BQ164947.1|BQ164947 EST610816 KVKC Medicago truncatula cDNA clone pKVKC-3H9, mRNA
sequence
Length = 788
Score = 61.6 bits (128), Expect(3) = 2e-024
Identities = 22/30 (73%), Positives = 24/30 (80%)
Frame = -2 / +1
Query: 153 GVYSEVYRVLKPGQYFALDEWCLTDLFDPN 64
G Y E+YRVLKPGQ FA EWC+TD FDPN
Sbjct: 688 GCYKEIYRVLKPGQCFAAYEWCMTDSFDPN 777
Score = 61.6 bits (128), Expect(3) = 2e-024
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = -2 / +1
Query: 360 GLSEQCCSLKGDFMNMPIADNTFDAAYAIQATCYAPEA 247
G+ + C +K DFM MP DN+FDA YAI+ATC+AP+A
Sbjct: 571 GVDKTCNFVKADFMKMPFEDNSFDAVYAIEATCHAPDA 684
Score = 28.1 bits (55), Expect(3) = 2e-024
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2 / +1
Query: 513 SSTXXXGLXXNDYHVSRGK 457
SST GL N+Y ++RGK
Sbjct: 496 SSTSVTGLNNNEYQITRGK 552
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 556,179,040
Number of Sequences: 392609
Number of extensions: 7620943
Number of successful extensions: 39267
Number of sequences better than 1.0e-020: 11
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 20923211870
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)