TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBN10e11.xg.2.2
(581 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB891199.1|CB891199 EST648168 KV3 Medicago truncatula cD... 122 7e-035
gb|BF648256.1|BF648256 NF045H03EC1F1031 Elicited cell cultu... 122 7e-035
gb|BE322931.1|BE322931 NF025A06IN1F1038 Insect herbivory Me... 122 7e-035
gb|BI271778.1|BI271778 NF019A05FL1F1036 Developing flower M... 122 8e-035
gb|BQ164947.1|BQ164947 EST610816 KVKC Medicago truncatula c... 122 9e-035
gb|AW559881.1|AW559881 EST314929 DSIR Medicago truncatula c... 108 1e-030
gb|CB894252.1|CB894252 EST647044 HOGA Medicago truncatula c... 88 2e-024
gb|AC142396.25| Medicago truncatula clone mth2-6g9, complet... 51 4e-024
>gb|CB891199.1|CB891199 EST648168 KV3 Medicago truncatula cDNA clone KV3-49B3, mRNA
sequence
Length = 624
Score = 122 bits (262), Expect(2) = 7e-035
Identities = 48/66 (72%), Positives = 58/66 (87%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RFSSTS+TGL NN+Y
Sbjct: 188 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVTGLNNNEY 367
Query: 396 HVSRGK 413
++RGK
Sbjct: 368 QITRGK 385
Score = 95.9 bits (203), Expect(2) = 8e-022
Identities = 40/73 (54%), Positives = 48/73 (65%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W SLL NPV +VELN IS+ GPPIP PTS+TF P + P+C A KC CRLM
Sbjct: 396 RLSAFPLVIWYSLLFNPVTDVELNREISLNGPPIPQPTSKTFCPGVRPSCKARKCSCRLM 217
Query: 244 ASREVSPGYLKYK 206
S SP +L K
Sbjct: 216 LSLRDSPTHLVAK 178
Score = 46.9 bits (96), Expect(2) = 7e-035
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 128 YDLATSFYEFGWGESFHFA 184
Score = 29.9 bits (59), Expect(2) = 8e-022
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K V RS*
Sbjct: 183 AKWKDSPQPNS*KLVARS* 127
>gb|BF648256.1|BF648256 NF045H03EC1F1031 Elicited cell culture Medicago truncatula cDNA
clone NF045H03EC 5', mRNA sequence
Length = 635
Score = 122 bits (262), Expect(2) = 7e-035
Identities = 48/66 (72%), Positives = 58/66 (87%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RFSSTS+TGL NN+Y
Sbjct: 284 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVTGLNNNEY 463
Query: 396 HVSRGK 413
++RGK
Sbjct: 464 QITRGK 481
Score = 95.9 bits (203), Expect(2) = 8e-022
Identities = 40/73 (54%), Positives = 48/73 (65%)
Frame = -2 / -3
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W SLL NPV +VELN IS+ GPPIP PTS+TF P + P+C A KC CRLM
Sbjct: 492 RLSAFPLVIWYSLLFNPVTDVELNREISLNGPPIPQPTSKTFCPGVRPSCKARKCSCRLM 313
Query: 244 ASREVSPGYLKYK 206
S SP +L K
Sbjct: 312 LSLRDSPTHLVAK 274
Score = 46.9 bits (96), Expect(2) = 7e-035
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 224 YDLATSFYEFGWGESFHFA 280
Score = 29.9 bits (59), Expect(2) = 8e-022
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -2 / -3
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K V RS*
Sbjct: 279 AKWKDSPQPNS*KLVARS* 223
>gb|BE322931.1|BE322931 NF025A06IN1F1038 Insect herbivory Medicago truncatula cDNA clone
NF025A06IN 5', mRNA sequence
Length = 660
Score = 122 bits (262), Expect(2) = 7e-035
Identities = 48/66 (72%), Positives = 58/66 (87%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RFSSTS+TGL NN+Y
Sbjct: 257 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVTGLNNNEY 436
Query: 396 HVSRGK 413
++RGK
Sbjct: 437 QITRGK 454
Score = 95.9 bits (203), Expect(2) = 9e-022
Identities = 40/73 (54%), Positives = 48/73 (65%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W SLL NPV +VELN IS+ GPPIP PTS+TF P + P+C A KC CRLM
Sbjct: 465 RLSAFPLVIWYSLLFNPVTDVELNREISLNGPPIPQPTSKTFCPGVRPSCKARKCSCRLM 286
Query: 244 ASREVSPGYLKYK 206
S SP +L K
Sbjct: 285 LSLRDSPTHLVAK 247
Score = 46.9 bits (96), Expect(2) = 7e-035
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 197 YDLATSFYEFGWGESFHFA 253
Score = 29.9 bits (59), Expect(2) = 9e-022
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K V RS*
Sbjct: 252 AKWKDSPQPNS*KLVARS* 196
>gb|BI271778.1|BI271778 NF019A05FL1F1036 Developing flower Medicago truncatula cDNA clone
NF019A05FL 5', mRNA sequence
Length = 691
Score = 122 bits (262), Expect(2) = 8e-035
Identities = 48/66 (72%), Positives = 58/66 (87%)
Frame = +3 / +2
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RFSSTS+TGL NN+Y
Sbjct: 302 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVTGLNNNEY 481
Query: 396 HVSRGK 413
++RGK
Sbjct: 482 QITRGK 499
Score = 95.9 bits (203), Expect(2) = 9e-022
Identities = 40/73 (54%), Positives = 48/73 (65%)
Frame = -2 / -2
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W SLL NPV +VELN IS+ GPPIP PTS+TF P + P+C A KC CRLM
Sbjct: 510 RLSAFPLVIWYSLLFNPVTDVELNREISLNGPPIPQPTSKTFCPGVRPSCKARKCSCRLM 331
Query: 244 ASREVSPGYLKYK 206
S SP +L K
Sbjct: 330 LSLRDSPTHLVAK 292
Score = 46.9 bits (96), Expect(2) = 8e-035
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +2
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 242 YDLATSFYEFGWGESFHFA 298
Score = 29.9 bits (59), Expect(2) = 9e-022
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -2 / -2
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K V RS*
Sbjct: 297 AKWKDSPQPNS*KLVARS* 241
>gb|BQ164947.1|BQ164947 EST610816 KVKC Medicago truncatula cDNA clone pKVKC-3H9, mRNA
sequence
Length = 788
Score = 122 bits (262), Expect(2) = 9e-035
Identities = 48/66 (72%), Positives = 58/66 (87%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNNDY 395
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RFSSTS+TGL NN+Y
Sbjct: 355 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRFSSTSVTGLNNNEY 534
Query: 396 HVSRGK 413
++RGK
Sbjct: 535 QITRGK 552
Score = 95.9 bits (203), Expect(2) = 1e-021
Identities = 40/73 (54%), Positives = 48/73 (65%)
Frame = -2 / -1
Query: 424 KMQTLPLETW*SLLVNPVIEVELNLAISIKGPPIPHPTSRTFIPFLSPNCSAIKCWCRLM 245
++ PL W SLL NPV +VELN IS+ GPPIP PTS+TF P + P+C A KC CRLM
Sbjct: 563 RLSAFPLVIWYSLLFNPVTDVELNREISLNGPPIPQPTSKTFCPGVRPSCKARKCSCRLM 384
Query: 244 ASREVSPGYLKYK 206
S SP +L K
Sbjct: 383 LSLRDSPTHLVAK 345
Score = 46.9 bits (96), Expect(2) = 9e-035
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 295 YDLATSFYEFGWGESFHFA 351
Score = 29.9 bits (59), Expect(2) = 1e-021
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 58 AKWNESPHPYS*KPVTRS* 2
AKW +SP P S*K V RS*
Sbjct: 350 AKWKDSPQPNS*KLVARS* 294
>gb|AW559881.1|AW559881 EST314929 DSIR Medicago truncatula cDNA clone pDSIR-25B19, mRNA
sequence
Length = 500
Score = 108 bits (230), Expect(2) = 1e-030
Identities = 42/59 (71%), Positives = 52/59 (88%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARFSSTSITGLTNND 392
R+ GE+ +E+IKRH+HF+ALQLGL G KVLDVGCG+GGPL EI+RFSSTS+TGL NN+
Sbjct: 322 RWVGESLKESIKRHEHFLALQLGLTPGQKVLDVGCGLGGPLREISRFSSTSVTGLNNNE 498
Score = 46.9 bits (96), Expect(2) = 1e-030
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 262 YDLATSFYEFGWGESFHFA 318
>gb|CB894252.1|CB894252 EST647044 HOGA Medicago truncatula cDNA clone HOGA-30H7, mRNA
sequence
Length = 792
Score = 88.1 bits (186), Expect(2) = 2e-024
Identities = 35/47 (74%), Positives = 41/47 (87%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKVLDVGCGIGGPLIEIARF 356
R+ GE+ RE+IKRH+HF+ALQLGL G KVLDVGCGIGGPL EI+RF
Sbjct: 232 RWVGESLRESIKRHEHFLALQLGLTPGQKVLDVGCGIGGPLREISRF 372
Score = 46.9 bits (96), Expect(2) = 2e-024
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFA 59
YDL T FYE+GWG+SFHFA
Sbjct: 172 YDLATSFYEFGWGESFHFA 228
>gb|AC142396.25| Medicago truncatula clone mth2-6g9, complete sequence
Length = 106058
Score = 51.5 bits (106), Expect(4) = 4e-024
Identities = 20/30 (66%), Positives = 25/30 (83%)
Frame = +3 / +1
Query: 216 RYPGETSREAIKRHQHFMALQLGLKKGMKV 305
R+ GE+ RE+IKRH+HF+ALQLGL G KV
Sbjct: 54709 RWVGESLRESIKRHEHFLALQLGLTPGQKV 54798
Score = 50.6 bits (104), Expect(4) = 4e-024
Identities = 18/32 (56%), Positives = 22/32 (68%)
Frame = +3 / +1
Query: 3 YDLVTGFYEYGWGDSFHFAGGSATYAYLXXXI 98
YDL T FYE+GWG+SFHFA Y++L I
Sbjct: 54565 YDLATSFYEFGWGESFHFATRCPFYSFLHIQI 54660
Score = 46.9 bits (96), Expect(4) = 4e-024
Identities = 16/23 (69%), Positives = 21/23 (91%)
Frame = +3 / +3
Query: 357 SSTSITGLTNNDYHVSRGKVCIL 425
SSTS+TGL NN+Y ++RGKVC+L
Sbjct: 55404 SSTSVTGLNNNEYQITRGKVCVL 55472
Score = 41.4 bits (84), Expect(4) = 4e-024
Identities = 16/18 (88%), Positives = 17/18 (94%)
Frame = +3 / +3
Query: 303 VLDVGCGIGGPLIEIARF 356
VLDVGCGIGGPL EI+RF
Sbjct: 54903 VLDVGCGIGGPLREISRF 54956
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 551,870,895
Number of Sequences: 392609
Number of extensions: 7559581
Number of successful extensions: 51452
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 17701047560
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)