TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAF14f01.yg.2.1
(319 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA990579.1|CA990579 EST644087 GESD Medicago truncatula c... 72 2e-023
gb|CX538323.1|CX538323 s13dNF80A11GS081_459898 Germinating ... 72 3e-023
gb|BI272689.1|BI272689 NF024D06FL1F1057 Developing flower M... 72 3e-023
gb|CA990448.1|CA990448 EST643956 GESD Medicago truncatula c... 72 1e-022
>gb|CA990579.1|CA990579 EST644087 GESD Medicago truncatula cDNA clone GESD-29M9, mRNA
sequence
Length = 679
Score = 72.1 bits (151), Expect(2) = 2e-023
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNP ACAWAYG+N F L R CTE +H + +NE+ LW
Sbjct: 188 YMNSSHPLIKAKFNPKACAWAYGMNFFDLDAWRRVKCTEEYHYWQNLNENRTLW 349
Score = 55.6 bits (115), Expect(2) = 2e-023
Identities = 23/40 (57%), Positives = 26/40 (65%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I +AAV
Sbjct: 362 LPPGLITYYSTTKPLDKSWHVLGLGYNPSISMDEINNAAV 481
>gb|CX538323.1|CX538323 s13dNF80A11GS081_459898 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 661
Score = 71.6 bits (150), Expect(2) = 3e-023
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +2
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNP ACAWAYG+N F L R CTE +H + +NE+ LW
Sbjct: 338 YMNFSHPLIKAKFNPKACAWAYGMNFFDLDAWRRVKCTEEYHYWQNLNENRTLW 499
Score = 55.6 bits (115), Expect(2) = 3e-023
Identities = 23/40 (57%), Positives = 26/40 (65%)
Frame = -3 / +2
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I +AAV
Sbjct: 512 LPPGLITYYSTTKPLDKSWHVLGLGYNPSISMDEINNAAV 631
>gb|BI272689.1|BI272689 NF024D06FL1F1057 Developing flower Medicago truncatula cDNA clone
NF024D06FL 5', mRNA sequence
Length = 724
Score = 71.6 bits (150), Expect(2) = 3e-023
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNP ACAWAYG+N F L R CTE +H + +NE+ LW
Sbjct: 75 YMNFSHPLIKAKFNPKACAWAYGMNFFDLDAWRRVKCTEEYHYWQNLNENRTLW 236
Score = 55.6 bits (115), Expect(2) = 3e-023
Identities = 23/40 (57%), Positives = 26/40 (65%)
Frame = -3 / +3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+GLGYNPSIS + I +AAV
Sbjct: 249 LPPGLITYYSTTKPLDKSWHVLGLGYNPSISMDEINNAAV 368
>gb|CA990448.1|CA990448 EST643956 GESD Medicago truncatula cDNA clone GESD-28P9, mRNA
sequence
Length = 695
Score = 71.6 bits (150), Expect(2) = 1e-022
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = -3 / +3
Query: 317 YLXXXXPIVRDRFNPNACAWAYGVNVFXLXXXXRDGCTELFHQYMEMNEDXELW 156
Y+ P+++ +FNP ACAWAYG+N F L R CTE +H + +NE+ LW
Sbjct: 54 YMNFSHPLIKAKFNPKACAWAYGMNFFDLDAWRRVKCTEEYHYWQNLNENRTLW 215
Score = 53.8 bits (111), Expect(2) = 1e-022
Identities = 22/40 (55%), Positives = 25/40 (62%)
Frame = -3 / +3
Query: 140 LTAGXXXXXGNTKXXDKSWHVMGLGYNPSISPEAIRSAAV 21
L G TK DKSWHV+G GYNPSIS + I +AAV
Sbjct: 228 LPPGLITYYSTTKPLDKSWHVLGFGYNPSISMDEINNAAV 347
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 263,558,439
Number of Sequences: 392609
Number of extensions: 2540463
Number of successful extensions: 82192
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 51
effective length of database: 127,221,272
effective search space used: 6869948688
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)