TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE36e08.yg.2.1
(352 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC149303.10| Medicago truncatula clone mth2-76a20, compl... 101 8e-039
emb|CT009652.1| Medicago truncatula chromosome 5 clone mth2... 108 2e-038
gb|BI272580.1|BI272580 NF024B08FL1F1064 Developing flower M... 101 2e-033
gb|CG960737.1|CG960737 MBEKQ86TR mth2 Medicago truncatula g... 73 2e-025
emb|CT033766.1| Medicago truncatula chromosome 5 clone mth4... 81 2e-025
gb|CA921793.1|CA921793 EST639511 MTUS Medicago truncatula c... 108 4e-025
gb|CB891497.1|CB891497 EST648466 KV3 Medicago truncatula cD... 108 4e-023
gb|BI309278.1|BI309278 EST530688 GPOD Medicago truncatula c... 78 3e-022
gb|CG956421.1|CG956421 MBELT33TR mth2 Medicago truncatula g... 72 2e-021
>gb|AC149303.10| Medicago truncatula clone mth2-76a20, complete sequence
Length = 141028
Score = 101 bits (215), Expect(2) = 8e-039
Identities = 35/59 (59%), Positives = 47/59 (79%)
Frame = -1 / -1
Query: 181 NVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYD 5
NV R+LWQPV GT+ Y++++G+ VQIV QDT I E HP+H+HGY FY++A GFGN+D
Sbjct: 40636 NVSRSLWQPVPGTKAYKLKFGSRVQIVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNFD 40460
Score = 79.0 bits (166), Expect(2) = 8e-039
Identities = 31/43 (72%), Positives = 35/43 (81%)
Frame = -1 / -1
Query: 316 ASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFT 188
ASM VSF LP+ +S+LQAHYH IPGVFT DFPA PPV FD+T
Sbjct: 40768 ASMNNVSFTLPNNISILQAHYHGIPGVFTTDFPAKPPVKFDYT 40640
>emb|CT009652.1| Medicago truncatula chromosome 5 clone mth2-124p21, WORKING DRAFT
SEQUENCE
Length = 110957
Score = 108 bits (230), Expect(2) = 2e-038
Identities = 36/60 (60%), Positives = 50/60 (83%)
Frame = -1 / -2
Query: 181 NVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
NVPR LW P KGT+L++++YG+ VQIV QDT I E+HPMH+HG+HF+V+ +GFGN++P
Sbjct: 76690 NVPRGLWTPRKGTKLFKLKYGSNVQIVLQDTSIVTVEDHPMHVHGFHFFVVGSGFGNFNP 76511
Score = 70.7 bits (148), Expect(2) = 2e-038
Identities = 27/55 (49%), Positives = 37/55 (67%)
Frame = -1 / -2
Query: 352 PFCQGXXXXXXGASMXXVSFQLPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFT 188
P CQG AS+ SF LP T SL+QA+Y+ +PG+FT DFP +PP+ F++T
Sbjct: 76858 PRCQGPNGTRFAASINNNSFVLPRTTSLMQAYYNGVPGIFTTDFPPVPPIQFNYT 76694
>gb|BI272580.1|BI272580 NF024B08FL1F1064 Developing flower Medicago truncatula cDNA clone
NF024B08FL 5', mRNA sequence
Length = 640
Score = 101 bits (215), Expect(3) = 2e-033
Identities = 35/59 (59%), Positives = 47/59 (79%)
Frame = -1 / +2
Query: 181 NVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYD 5
NV R+LWQPV GT+ Y++++G+ VQIV QDT I E HP+H+HGY FY++A GFGN+D
Sbjct: 119 NVSRSLWQPVPGTKAYKLKFGSRVQIVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNFD 295
Score = 49.2 bits (101), Expect(3) = 2e-033
Identities = 19/27 (70%), Positives = 22/27 (81%)
Frame = -1 / +1
Query: 301 VSFQLPDTVSLLQAHYHHIPGVFTDDF 221
VSF LP+ +S+LQAHYH IPGVFT F
Sbjct: 1 VSFTLPNNISILQAHYHGIPGVFTT*F 81
Score = 28.6 bits (56), Expect(3) = 2e-033
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = -1 / +2
Query: 226 DFPALPPVFFDFT 188
DFPA PPV FD+T
Sbjct: 77 DFPAKPPVKFDYT 115
>gb|CG960737.1|CG960737 MBEKQ86TR mth2 Medicago truncatula genomic clone 76O3, DNA sequence
Length = 927
Score = 73.5 bits (154), Expect(2) = 2e-025
Identities = 24/49 (48%), Positives = 36/49 (73%)
Frame = -1 / +1
Query: 148 GTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
GT++YR+ + + VQIV Q T + A E HP H+HG++F+V+ G GN+DP
Sbjct: 166 GTKVYRLNFNSSVQIVLQGTAMIAPENHPFHLHGFNFFVVGQGLGNFDP 312
Score = 62.5 bits (130), Expect(2) = 2e-025
Identities = 25/42 (59%), Positives = 32/42 (76%)
Frame = -1 / +1
Query: 289 LPDTVSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVPRAL 164
L TVSLLQA+Y++I GVFTDDFP+ PP+ FD+T + P L
Sbjct: 28 LMPTVSLLQANYYNIKGVFTDDFPSKPPMVFDYTGTDQPANL 153
>emb|CT033766.1| Medicago truncatula chromosome 5 clone mth4-42c9, WORKING DRAFT SEQUENCE
Length = 235225
Score = 80.8 bits (170), Expect(2) = 2e-025
Identities = 26/50 (52%), Positives = 40/50 (80%)
Frame = -1 / -1
Query: 151 KGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
KGTR+YR+ Y + VQ++ QDTG+ A E HP+H+HG++F+V+ G GN++P
Sbjct: 168769 KGTRVYRLAYNSTVQLILQDTGMIAPENHPIHLHGFNFFVVGKGQGNFNP 168620
Score = 54.7 bits (113), Expect(2) = 2e-025
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = -1 / -1
Query: 277 VSLLQAHYHHIPGVFTDDFPALPPVFFDFTSQNVP 173
+SLLQAH+ +I G+FTDDFP P + +DFT P
Sbjct: 168892 ISLLQAHFFNISGIFTDDFPRKPEIVYDFTGNKQP 168788
>gb|CA921793.1|CA921793 EST639511 MTUS Medicago truncatula cDNA clone MTUS-44E1, mRNA
sequence
Length = 763
Score = 108 bits (230), Expect(2) = 4e-025
Identities = 36/60 (60%), Positives = 50/60 (83%)
Frame = -1 / -3
Query: 181 NVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
NVPR LW P KGT+L++++YG+ VQIV QDT I E+HPMH+HG+HF+V+ +GFGN++P
Sbjct: 659 NVPRGLWTPRKGTKLFKLKYGSNVQIVLQDTSIVTVEDHPMHVHGFHFFVVGSGFGNFNP 480
Score = 26.3 bits (51), Expect(2) = 4e-025
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -1 / -1
Query: 289 LPDTVSLLQAHYHHIPGVFTDDF 221
LP L+QA+Y+ +PG+ F
Sbjct: 763 LPRQTPLMQAYYNGVPGILPQIF 695
>gb|CB891497.1|CB891497 EST648466 KV3 Medicago truncatula cDNA clone KV3-50D17, mRNA
sequence
Length = 593
Score = 108 bits (230), Expect = 4e-023
Identities = 36/60 (60%), Positives = 50/60 (83%)
Frame = -1 / -2
Query: 181 NVPRALWQPVKGTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
NVPR LW P KGT+L++++YG+ VQIV QDT I E+HPMH+HG+HF+V+ +GFGN++P
Sbjct: 562 NVPRGLWTPRKGTKLFKLKYGSNVQIVLQDTSIVTVEDHPMHVHGFHFFVVGSGFGNFNP 383
>gb|BI309278.1|BI309278 EST530688 GPOD Medicago truncatula cDNA clone pGPOD-11O15 5' end,
mRNA sequence
Length = 615
Score = 77.6 bits (163), Expect(2) = 3e-022
Identities = 26/48 (54%), Positives = 37/48 (77%)
Frame = -1 / +1
Query: 148 GTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYD 5
GT+LY++ + VQ+V QDTGI A E HP+H+HG++F+V+ G GNYD
Sbjct: 469 GTKLYKLSFNDTVQLVMQDTGIIAPESHPVHLHGFNFFVVGRGVGNYD 612
Score = 46.4 bits (95), Expect(2) = 3e-022
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = -1 / +1
Query: 280 TVSLLQAHYHHIPGVFTDDFPALPPVFFDFT 188
T +LLQAHY +I VFT DFP PP ++FT
Sbjct: 343 TTALLQAHYFNIKNVFTADFPPNPPHIYNFT 435
>gb|CG956421.1|CG956421 MBELT33TR mth2 Medicago truncatula genomic clone 82F18, DNA
sequence
Length = 666
Score = 71.6 bits (150), Expect(2) = 2e-021
Identities = 23/49 (46%), Positives = 36/49 (73%)
Frame = -1 / +2
Query: 148 GTRLYRVRYGAVVQIVFQDTGIFAAEEHPMHIHGYHFYVLATGFGNYDP 2
GT++YR+ + + VQIV Q T + A + HP H+HG++F+V+ G GN+DP
Sbjct: 113 GTKVYRLNFNSSVQIVLQGTAMIAPKNHPFHLHGFNFFVVGQGLGNFDP 259
Score = 50.1 bits (103), Expect(2) = 2e-021
Identities = 18/33 (54%), Positives = 25/33 (75%)
Frame = -1 / +2
Query: 262 AHYHHIPGVFTDDFPALPPVFFDFTSQNVPRAL 164
A+Y++I GVFTDDFP+ PP+ FD+T + P L
Sbjct: 2 ANYYNIKGVFTDDFPSKPPMVFDYTGTDQPANL 100
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 198,606,430
Number of Sequences: 392609
Number of extensions: 2115480
Number of successful extensions: 71909
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 51
effective length of database: 127,221,272
effective search space used: 8269382680
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)