TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704580.2.1
(721 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CX539845.1|CX539845 s13dNF0HG12GS088_462962 Germinating ... 292 6e-078
gb|BI311789.1|BI311789 EST5313539 GESD Medicago truncatula ... 269 1e-072
gb|AC119419.9| Medicago truncatula clone mth2-6b12, complet... 84 4e-051
gb|AC127674.21| Medicago truncatula clone mth2-20a12, WORKI... 84 8e-051
gb|AW329423.2|AW329423 N200658e rootphos(-) Medicago trunca... 189 5e-047
gb|BF518932.1|BF518932 EST456464 DSIL Medicago truncatula c... 119 4e-026
emb|CR317587.1| mte1-42K1RM1 BAC end, cultivar Jemalong A17... 84 4e-021
>gb|CX539845.1|CX539845 s13dNF0HG12GS088_462962 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 636
Score = 292 bits (631), Expect = 6e-078
Identities = 117/145 (80%), Positives = 136/145 (93%)
Frame = -3 / +3
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILAD 513
VEAC+EET+VVYVD LL+QKNYIKEETIERMRLDEEV+MDFFREHI+V+KVE+RV +L+D
Sbjct: 165 VEACLEETVVVYVDRLLTQKNYIKEETIERMRLDEEVIMDFFREHISVSKVENRVSVLSD 344
Query: 512 MRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGIPKKEAKEVVQECKEIYEN 333
+RELASAESLD+FTLIYTNILEHQPDCPPEV KLV +REGIP+K+AKEV+QECK+IYEN
Sbjct: 345 LRELASAESLDTFTLIYTNILEHQPDCPPEVVEKLVGLREGIPRKDAKEVIQECKDIYEN 524
Query: 332 SLVDGNPQKSGFVFGKLKCLTAKKG 258
SLVDG P K+GFVF ++KCLTA KG
Sbjct: 525 SLVDGRPPKTGFVFHRVKCLTASKG 599
Score = 149 bits (320), Expect = 5e-035
Identities = 68/139 (48%), Positives = 92/139 (66%)
Frame = +3 / -2
Query: 276 ALQFAKHKTGLLRVAIHKRVLVYLFAFLYNLLGFLFWNAFPHCHKLIKNLRRAIRLMFQY 455
AL K+KT L R +I+KR+ +Y+FA LY+LLG L N+F +K + NLRRA+RLMF+Y
Sbjct: 581 ALHPMKNKTCLWRSSINKRIFIYVFALLYDLLGILPRNSFS*TNKFLNNLRRAVRLMFEY 402
Query: 456 ISVNESKAIQTLS*S*LPHICQNPHSAFNFCHVDVFPEEIHKHLFI*PHPLYRFFFYVVF 635
I ++E K IQ L *S P + + HS FNF + D+F +E+H L I* HP FF VVF
Sbjct: 401 ICIDECKGIQALR*SKFPQVAKYTHSVFNFRNTDMFSKEVHDDLLI*SHPFNSFFLDVVF 222
Query: 636 LRKEMVNIDHNCFLYASLH 692
L + VNI ++CF AS +
Sbjct: 221 LC*KTVNIYNHCFF*ASFN 165
Score = 147 bits (316), Expect = 2e-034
Identities = 76/145 (52%), Positives = 80/145 (55%)
Frame = +1 / -3
Query: 259 PFFAVKHFNLPNTKPDF*GLPSTSEFSYISLHSCTTSLASFFGMPSRIATSL*KTSGGQS 438
PF AVKHF TKP F GLPST EFSY+SLHSC TSLASF G+PSR TS TSGGQS
Sbjct: 598 PFEAVKHFTR*KTKPVFGGLPSTREFSYMSLHSCMTSLASFRGIPSRRPTSFSTTSGGQS 419
Query: 439 G*CSSILV*MRVXXXXXXXXXXXXXXXXXXTLLSTFVTLMCXXXXXXXXXXXXXXXXXXX 618
G*CSSI V*M V TL ST TL+C
Sbjct: 418 G*CSSIFV*MSVKVSRLSAEASSLKSLSTLTLFSTLETLICSLKKSMMTSSSSLIRSIVS 239
Query: 619 XFM*FF*ERRWST*TTIVSSMQAST 693
M*FF +R ST TT VSS QAST
Sbjct: 238 SLM*FFCVKRRSTYTTTVSSRQAST 164
Score = 98.2 bits (208), Expect(2) = 1e-021
Identities = 53/105 (50%), Positives = 67/105 (63%)
Frame = -2 / +2
Query: 585 GAYGFLQGTHQRDKS*KQSADSGRYEGASFS*EFG*LYSHLH*YTGTST*LPA*GFL*AC 406
G +G L T+Q S*KQS + R EG FS E G LY+HL+ YT TST*LPA*G C
Sbjct: 272 GHHGLL*RTYQCF*S*KQSECT*RLEGTCFSGEPGYLYTHLYKYTRTST*LPA*GC*ETC 451
Query: 405 GNAGRHSKKGSQGGCTRMQRDIRELACGWQPSEVRFCVWQIEVLD 271
+ R+S +G QGG TR+QR I + +C W+ S+ RFC EVL+
Sbjct: 452 WSTRRNSSEGCQGGHTRVQRHI*KFSC*WKTSKDRFCFSSGEVLN 586
Score = 26.3 bits (51), Expect(2) = 1e-021
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -2 / +2
Query: 693 CGGLHRGNNCGLC*PSPFSEKLHK 622
C L R N+ +C*PS +EKLH+
Sbjct: 164 C*SLPRRNSGCIC*PSFNTEKLHQ 235
>gb|BI311789.1|BI311789 EST5313539 GESD Medicago truncatula cDNA clone pGESD15O10 5' end,
mRNA sequence
Length = 688
Score = 269 bits (581), Expect(2) = 1e-072
Identities = 108/134 (80%), Positives = 125/134 (93%)
Frame = -3 / +1
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILAD 513
VEAC+EET+VVYVD LL+QKNYIKEETIERMRLDEEV+MDFFREHI+V+KVE+RV +L+D
Sbjct: 163 VEACLEETVVVYVDRLLTQKNYIKEETIERMRLDEEVIMDFFREHISVSKVENRVSVLSD 342
Query: 512 MRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGIPKKEAKEVVQECKEIYEN 333
+RELASAESLD+FTLIYTNILEHQPDCPPEV KLV +REGIP+K+AKEV+QECK+IYEN
Sbjct: 343 LRELASAESLDTFTLIYTNILEHQPDCPPEVVEKLVGLREGIPRKDAKEVIQECKDIYEN 522
Query: 332 SLVDGNPQKSGFVF 291
SLVDG P K+G F
Sbjct: 523 SLVDGRPPKTGLFF 564
Score = 142 bits (304), Expect = 7e-033
Identities = 63/129 (48%), Positives = 86/129 (66%)
Frame = +3 / -2
Query: 306 LLRVAIHKRVLVYLFAFLYNLLGFLFWNAFPHCHKLIKNLRRAIRLMFQYISVNESKAIQ 485
L R +I+KR+ +Y+FA LY+LLG L N+F +K + NLRRA+RLMF+YI ++E K IQ
Sbjct: 549 LWRSSINKRIFIYVFALLYDLLGILPRNSFS*TNKFLNNLRRAVRLMFEYICIDECKGIQ 370
Query: 486 TLS*S*LPHICQNPHSAFNFCHVDVFPEEIHKHLFI*PHPLYRFFFYVVFLRKEMVNIDH 665
L *S P + + HS FNF + D+F +E+H L I* HP FF VVFL + VNI +
Sbjct: 369 ALR*SKFPQVAKYTHSVFNFRNTDMFSKEVHDDLLI*SHPFNSFFLDVVFLC*KTVNIYN 190
Query: 666 NCFLYASLH 692
+CF AS +
Sbjct: 189 HCFF*ASFN 163
Score = 128 bits (275), Expect = 7e-029
Identities = 67/131 (51%), Positives = 71/131 (54%)
Frame = +1 / -3
Query: 301 PDF*GLPSTSEFSYISLHSCTTSLASFFGMPSRIATSL*KTSGGQSG*CSSILV*MRVXX 480
P F GLPST EFSY+SLHSC TSLASF G+PSR TS TSGGQSG*CSSI V*M V
Sbjct: 554 PVFGGLPSTREFSYMSLHSCMTSLASFRGIPSRRPTSFSTTSGGQSG*CSSIFV*MSVKV 375
Query: 481 XXXXXXXXXXXXXXXXTLLSTFVTLMCXXXXXXXXXXXXXXXXXXXXFM*FF*ERRWST* 660
TL ST TL+C M*FF +R ST
Sbjct: 374 SRLSAEASSLKSLSTLTLFSTLETLICSLKKSMMTSSSSLIRSIVSSLM*FFCVKRRSTY 195
Query: 661 TTIVSSMQAST 693
TT VSS QAST
Sbjct: 194 TTTVSSRQAST 162
Score = 26.3 bits (51), Expect(2) = 1e-072
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3 / +3
Query: 308 KSGFVFGKLKCLTAKKG 258
K VF ++KCLTA KG
Sbjct: 546 KDRVVFHRVKCLTASKG 596
>gb|AC119419.9| Medicago truncatula clone mth2-6b12, complete sequence
Length = 106395
Score = 84.0 bits (177), Expect(5) = 4e-051
Identities = 33/39 (84%), Positives = 38/39 (97%)
Frame = -3 / +2
Query: 545 KVESRVRILADMRELASAESLDSFTLIYTNILEHQPDCP 429
KVE+RV +L+D+RELASAESLD+FTLIYTNILEHQPDCP
Sbjct: 25466 KVENRVSVLSDLRELASAESLDTFTLIYTNILEHQPDCP 25582
Score = 73.5 bits (154), Expect(5) = 4e-051
Identities = 27/37 (72%), Positives = 33/37 (89%)
Frame = -3 / +2
Query: 368 EVVQECKEIYENSLVDGNPQKSGFVFGKLKCLTAKKG 258
+V+QECK+IYENSLVDG P K+GFVF ++KCLTA KG
Sbjct: 26336 QVIQECKDIYENSLVDGRPPKTGFVFHRVKCLTASKG 26446
Score = 70.7 bits (148), Expect(5) = 4e-051
Identities = 28/31 (90%), Positives = 31/31 (100%)
Frame = -3 / +2
Query: 638 QKNYIKEETIERMRLDEEVLMDFFREHINVT 546
QKNYIKEETIERMRLDEEV+MDFFREHI+V+
Sbjct: 25250 QKNYIKEETIERMRLDEEVIMDFFREHISVS 25342
Score = 61.6 bits (128), Expect(4) = 1e-021
Identities = 26/36 (72%), Positives = 27/36 (75%)
Frame = +1 / -1
Query: 259 PFFAVKHFNLPNTKPDF*GLPSTSEFSYISLHSCTT 366
PF AVKHF TKP F GLPST EFSY+SLHSC T
Sbjct: 26445 PFEAVKHFTR*KTKPVFGGLPSTREFSYMSLHSCMT 26338
Score = 52.4 bits (108), Expect(4) = 1e-021
Identities = 21/39 (53%), Positives = 27/39 (69%)
Frame = +3 / -3
Query: 429 RAIRLMFQYISVNESKAIQTLS*S*LPHICQNPHSAFNF 545
RA+RLMF+YI ++E K IQ L *S P + + HS FNF
Sbjct: 25582 RAVRLMFEYICIDECKGIQALR*SKFPQVAKYTHSVFNF 25466
Score = 40.5 bits (82), Expect(5) = 4e-051
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -3 / +2
Query: 428 PEVFYKLVAMREGIPKKEAKEV 363
PEV KLV +REGIP+K+AKEV
Sbjct: 25832 PEVVEKLVGLREGIPRKDAKEV 25897
Score = 40.0 bits (81), Expect(5) = 4e-051
Identities = 15/19 (78%), Positives = 18/19 (94%)
Frame = -3 / +1
Query: 692 VEACIEETIVVYVDHLLSQ 636
VEAC+EET+VVYVD LL+Q
Sbjct: 25042 VEACLEETVVVYVDRLLTQ 25098
Score = 38.2 bits (77), Expect(4) = 1e-021
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +3 / -3
Query: 549 HVDVFPEEIHKHLFI*PHPLYRFFFYVVFLRKEM 650
+ D+F +E+H L I* HP FF VVFL+ ++
Sbjct: 25339 NTDMFSKEVHDDLLI*SHPFNSFFLDVVFLQLKL 25238
Score = 31.3 bits (62), Expect(4) = 1e-021
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +1 / -1
Query: 364 TSLASFFGMPSRIATSL*KTSG 429
TSLASF G+PSR TS TSG
Sbjct: 25896 TSLASFRGIPSRRPTSFSTTSG 25831
>gb|AC127674.21| Medicago truncatula clone mth2-20a12, WORKING DRAFT SEQUENCE, 6
unordered pieces
Length = 126913
Score = 84.0 bits (177), Expect(5) = 8e-051
Identities = 33/39 (84%), Positives = 38/39 (97%)
Frame = -3 / +3
Query: 545 KVESRVRILADMRELASAESLDSFTLIYTNILEHQPDCP 429
KVE+RV +L+D+RELASAESLD+FTLIYTNILEHQPDCP
Sbjct: 37149 KVENRVSVLSDLRELASAESLDTFTLIYTNILEHQPDCP 37265
Score = 73.5 bits (154), Expect(5) = 8e-051
Identities = 27/37 (72%), Positives = 33/37 (89%)
Frame = -3 / +3
Query: 368 EVVQECKEIYENSLVDGNPQKSGFVFGKLKCLTAKKG 258
+V+QECK+IYENSLVDG P K+GFVF ++KCLTA KG
Sbjct: 38019 QVIQECKDIYENSLVDGRPPKTGFVFHRVKCLTASKG 38129
Score = 70.7 bits (148), Expect(5) = 8e-051
Identities = 28/31 (90%), Positives = 31/31 (100%)
Frame = -3 / +3
Query: 638 QKNYIKEETIERMRLDEEVLMDFFREHINVT 546
QKNYIKEETIERMRLDEEV+MDFFREHI+V+
Sbjct: 36933 QKNYIKEETIERMRLDEEVIMDFFREHISVS 37025
Score = 61.6 bits (128), Expect(4) = 2e-021
Identities = 26/36 (72%), Positives = 27/36 (75%)
Frame = +1 / -1
Query: 259 PFFAVKHFNLPNTKPDF*GLPSTSEFSYISLHSCTT 366
PF AVKHF TKP F GLPST EFSY+SLHSC T
Sbjct: 38128 PFEAVKHFTR*KTKPVFGGLPSTREFSYMSLHSCMT 38021
Score = 52.4 bits (108), Expect(4) = 2e-021
Identities = 21/39 (53%), Positives = 27/39 (69%)
Frame = +3 / -3
Query: 429 RAIRLMFQYISVNESKAIQTLS*S*LPHICQNPHSAFNF 545
RA+RLMF+YI ++E K IQ L *S P + + HS FNF
Sbjct: 37265 RAVRLMFEYICIDECKGIQALR*SKFPQVAKYTHSVFNF 37149
Score = 40.5 bits (82), Expect(5) = 8e-051
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -3 / +3
Query: 428 PEVFYKLVAMREGIPKKEAKEV 363
PEV KLV +REGIP+K+AKEV
Sbjct: 37515 PEVVEKLVGLREGIPRKDAKEV 37580
Score = 40.0 bits (81), Expect(5) = 8e-051
Identities = 15/19 (78%), Positives = 18/19 (94%)
Frame = -3 / +2
Query: 692 VEACIEETIVVYVDHLLSQ 636
VEAC+EET+VVYVD LL+Q
Sbjct: 36725 VEACLEETVVVYVDRLLTQ 36781
Score = 38.2 bits (77), Expect(4) = 2e-021
Identities = 15/34 (44%), Positives = 22/34 (64%)
Frame = +3 / -3
Query: 549 HVDVFPEEIHKHLFI*PHPLYRFFFYVVFLRKEM 650
+ D+F +E+H L I* HP FF VVFL+ ++
Sbjct: 37022 NTDMFSKEVHDDLLI*SHPFNSFFLDVVFLQLKL 36921
Score = 31.3 bits (62), Expect(4) = 2e-021
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +1 / -1
Query: 364 TSLASFFGMPSRIATSL*KTSG 429
TSLASF G+PSR TS TSG
Sbjct: 37579 TSLASFRGIPSRRPTSFSTTSG 37514
>gb|AW329423.2|AW329423 N200658e rootphos(-) Medicago truncatula cDNA clone MHRP-19H10,
mRNA sequence
Length = 672
Score = 189 bits (407), Expect = 5e-047
Identities = 75/96 (78%), Positives = 88/96 (91%)
Frame = -3 / +3
Query: 545 KVESRVRILADMRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGIPKKEAKE 366
KVE+RV +L+D+RELASAESLD+FTLIYTNILEHQPDCPPEV KLV +REGIP+K+AKE
Sbjct: 3 KVENRVSVLSDLRELASAESLDTFTLIYTNILEHQPDCPPEVVEKLVGLREGIPRKDAKE 182
Query: 365 VVQECKEIYENSLVDGNPQKSGFVFGKLKCLTAKKG 258
V+QECK+IYENSLVDG P K+GFVF ++KCLTA KG
Sbjct: 183 VIQECKDIYENSLVDGRPPKTGFVFHRVKCLTASKG 290
Score = 117 bits (250), Expect = 2e-025
Identities = 57/95 (60%), Positives = 59/95 (62%)
Frame = +1 / -3
Query: 259 PFFAVKHFNLPNTKPDF*GLPSTSEFSYISLHSCTTSLASFFGMPSRIATSL*KTSGGQS 438
PF AVKHF TKP F GLPST EFSY+SLHSC TSLASF G+PSR TS TSGGQS
Sbjct: 289 PFEAVKHFTR*KTKPVFGGLPSTREFSYMSLHSCMTSLASFRGIPSRRPTSFSTTSGGQS 110
Query: 439 G*CSSILV*MRVXXXXXXXXXXXXXXXXXXTLLST 543
G*CSSI V*M V TL ST
Sbjct: 109 G*CSSIFV*MSVKVSRLSAEASSLKSLSTLTLFST 5
Score = 104 bits (222), Expect = 1e-021
Identities = 46/90 (51%), Positives = 62/90 (68%)
Frame = +3 / -2
Query: 276 ALQFAKHKTGLLRVAIHKRVLVYLFAFLYNLLGFLFWNAFPHCHKLIKNLRRAIRLMFQY 455
AL K+KT L R +I+KR+ +Y+FA LY+LLG L N+F +K + NLRRA+RLMF+Y
Sbjct: 272 ALHPMKNKTCLWRSSINKRIFIYVFALLYDLLGILPRNSFS*TNKFLNNLRRAVRLMFEY 93
Query: 456 ISVNESKAIQTLS*S*LPHICQNPHSAFNF 545
I ++E K IQ L *S P + + HS FNF
Sbjct: 92 ICIDECKGIQALR*SKFPQVAKYTHSVFNF 3
>gb|BF518932.1|BF518932 EST456464 DSIL Medicago truncatula cDNA clone pDSIL-19M14, mRNA
sequence
Length = 593
Score = 119 bits (255), Expect = 4e-026
Identities = 48/59 (81%), Positives = 57/59 (96%)
Frame = -3 / +2
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILA 516
VEAC+EET+VVYVD LL+QKNYIKEETIERMRLDEEV+MDFFREHI+V+KVE+RV +L+
Sbjct: 416 VEACLEETVVVYVDRLLTQKNYIKEETIERMRLDEEVIMDFFREHISVSKVENRVSVLS 592
>emb|CR317587.1| mte1-42K1RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 697
Score = 84.0 bits (177), Expect(2) = 4e-021
Identities = 33/39 (84%), Positives = 38/39 (97%)
Frame = -3 / -1
Query: 545 KVESRVRILADMRELASAESLDSFTLIYTNILEHQPDCP 429
KVE+RV +L+D+RELASAESLD+FTLIYTNILEHQPDCP
Sbjct: 646 KVENRVSVLSDLRELASAESLDTFTLIYTNILEHQPDCP 530
Score = 40.5 bits (82), Expect(2) = 4e-021
Identities = 16/22 (72%), Positives = 19/22 (86%)
Frame = -3 / -1
Query: 428 PEVFYKLVAMREGIPKKEAKEV 363
PEV KLV +REGIP+K+AKEV
Sbjct: 280 PEVVEKLVGLREGIPRKDAKEV 215
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 754,099,748
Number of Sequences: 392609
Number of extensions: 11222226
Number of successful extensions: 72081
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 23318037325
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)