TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5110719.2.1
(581 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG453939.1|BG453939 NF096C06LF1F1039 Developing leaf Med... 90 4e-034
gb|BG453105.1|BG453105 NF090G12LF1F1097 Developing leaf Med... 90 5e-033
gb|BG450990.1|BG450990 NF097F03DT1F1029 Drought Medicago tr... 89 1e-030
gb|DW019329.1|DW019329 EST1228290 MTY Medicago truncatula c... 90 3e-030
gb|BG647218.1|BG647218 EST508837 HOGA Medicago truncatula c... 90 2e-028
>gb|BG453939.1|BG453939 NF096C06LF1F1039 Developing leaf Medicago truncatula cDNA clone
NF096C06LF 5', mRNA sequence
Length = 658
Score = 89.5 bits (189), Expect(3) = 3e-030
Identities = 48/107 (44%), Positives = 58/107 (54%)
Frame = -2 / -1
Query: 394 SDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDFTPVKRCCSSMIFWRYFP*ASIRXXXX 215
S +G + S N S + P G S+ TP+ RCCSS+IF++YFP*ASI
Sbjct: 502 SGSGVAGSVLLSSSCWNISPGVGADTPEPGASERTPLSRCCSSIIFFKYFP*ASILNCSC 323
Query: 214 XXXXXXXXSRFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPS 74
SRF TSI S AS IC P++PS S SP S LSV FPS
Sbjct: 322 LCTSNCSCSRFCTSI*SFSASVICIPDEPSRLESISPVSFLSVFFPS 182
Score = 68.0 bits (142), Expect(4) = 4e-034
Identities = 27/40 (67%), Positives = 35/40 (87%)
Frame = +3 / +3
Query: 66 ASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQK 185
+S +G KTD K+ GD+L+ L+GSSG+QI+EALKLQMEVQK
Sbjct: 174 SSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQK 293
Score = 57.4 bits (119), Expect(4) = 4e-034
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +3 / +3
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQF 347
AQGKYL+KIIEEQQRL+GV SE P G S D F
Sbjct: 345 AQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMF 455
Score = 53.8 bits (111), Expect(4) = 4e-034
Identities = 21/33 (63%), Positives = 25/33 (75%)
Frame = +3 / +3
Query: 480 SRNEPLTPDSNCQNGSPVASPNHERAAKRQRGS 578
S +EP+TPDS CQ GSP SPN ER+ K+QR S
Sbjct: 558 SHHEPMTPDSGCQVGSPADSPNGERSTKKQRVS 656
Score = 44.1 bits (90), Expect(3) = 3e-030
Identities = 19/30 (63%), Positives = 20/30 (66%)
Frame = -2 / -1
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVSGS 488
L RC L LS GL+ GEP WQ ESGV GS
Sbjct: 655 LTRCFLVDLSPLGLSAGEPTWQPESGVIGS 566
Score = 37.3 bits (75), Expect(3) = 3e-030
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -2 / -1
Query: 76 SVEASGIYFASLYFCK*LFTWYIV 5
S E SG YFAS YFCK L TW +V
Sbjct: 181 SDEQSGKYFASRYFCKWLLTW*MV 110
Score = 29.5 bits (58), Expect(4) = 4e-034
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2 / +2
Query: 2 INNIPCKKSLAEIQTSKVYS* 64
+N++PC++ AEI TSK+ +*
Sbjct: 107 LNHLPCQEPFAEIPTSKILT* 169
>gb|BG453105.1|BG453105 NF090G12LF1F1097 Developing leaf Medicago truncatula cDNA clone
NF090G12LF 5', mRNA sequence
Length = 661
Score = 89.5 bits (189), Expect(3) = 1e-028
Identities = 48/107 (44%), Positives = 58/107 (54%)
Frame = -2 / -1
Query: 394 SDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDFTPVKRCCSSMIFWRYFP*ASIRXXXX 215
S +G + S N S + P G S+ TP+ RCCSS+IF++YFP*ASI
Sbjct: 502 SGSGVAGSVLLSSSCWNISPGVGADTPEPGASERTPLSRCCSSIIFFKYFP*ASILNCSC 323
Query: 214 XXXXXXXXSRFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPS 74
SRF TSI S AS IC P++PS S SP S LSV FPS
Sbjct: 322 LCTSNCSCSRFCTSI*SFSASVICIPDEPSRLESISPVSFLSVFFPS 182
Score = 68.0 bits (142), Expect(4) = 5e-033
Identities = 27/40 (67%), Positives = 35/40 (87%)
Frame = +3 / +3
Query: 66 ASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQK 185
+S +G KTD K+ GD+L+ L+GSSG+QI+EALKLQMEVQK
Sbjct: 174 SSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQK 293
Score = 57.4 bits (119), Expect(4) = 5e-033
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +3 / +3
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQF 347
AQGKYL+KIIEEQQRL+GV SE P G S D F
Sbjct: 345 AQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMF 455
Score = 49.6 bits (102), Expect(4) = 5e-033
Identities = 20/33 (60%), Positives = 24/33 (72%)
Frame = +3 / +3
Query: 480 SRNEPLTPDSNCQNGSPVASPNHERAAKRQRGS 578
S +EP+TPDS CQ GS SPN ER+ K+QR S
Sbjct: 558 SHHEPMTPDSGCQVGSXADSPNGERSTKKQRVS 656
Score = 38.2 bits (77), Expect(3) = 1e-028
Identities = 17/30 (56%), Positives = 18/30 (60%)
Frame = -2 / -1
Query: 577 LPRCLLAALSWFGLATGEPFWQLESGVSGS 488
L RC L LS GL+ P WQ ESGV GS
Sbjct: 655 LTRCFLVDLSPLGLSAXXPTWQPESGVIGS 566
Score = 37.3 bits (75), Expect(3) = 1e-028
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -2 / -1
Query: 76 SVEASGIYFASLYFCK*LFTWYIV 5
S E SG YFAS YFCK L TW +V
Sbjct: 181 SDEQSGKYFASRYFCKWLLTW*MV 110
Score = 29.5 bits (58), Expect(4) = 5e-033
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2 / +2
Query: 2 INNIPCKKSLAEIQTSKVYS* 64
+N++PC++ AEI TSK+ +*
Sbjct: 107 LNHLPCQEPFAEIPTSKILT* 169
>gb|BG450990.1|BG450990 NF097F03DT1F1029 Drought Medicago truncatula cDNA clone NF097F03DT
5', mRNA sequence
Length = 646
Score = 88.6 bits (187), Expect(3) = 7e-026
Identities = 43/83 (51%), Positives = 51/83 (61%)
Frame = -2 / -1
Query: 322 EAPPAGVSDFTPVKRCCSSMIFWRYFP*ASIRXXXXXXXXXXXXSRFWTSICSLRASDIC 143
+ P G S+ TP+ RCCSS+IF++YFP*ASI SRF TSI S AS IC
Sbjct: 430 DTPEPGASERTPLSRCCSSIIFFKYFP*ASILNCSCLCTSNCSCSRFCTSI*SFSASVIC 251
Query: 142 NPEDPSSPASKSPGSLLSVLFPS 74
P++PS S SP S LSV FPS
Sbjct: 250 IPDEPSRLESISPVSFLSVFFPS 182
Score = 68.0 bits (142), Expect(4) = 1e-030
Identities = 27/40 (67%), Positives = 35/40 (87%)
Frame = +3 / +3
Query: 66 ASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQK 185
+S +G KTD K+ GD+L+ L+GSSG+QI+EALKLQMEVQK
Sbjct: 174 SSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQK 293
Score = 57.4 bits (119), Expect(4) = 1e-030
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +3 / +3
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQF 347
AQGKYL+KIIEEQQRL+GV SE P G S D F
Sbjct: 345 AQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMF 455
Score = 41.4 bits (84), Expect(4) = 1e-030
Identities = 15/25 (60%), Positives = 18/25 (72%)
Frame = +3 / +2
Query: 492 PLTPDSNCQNGSPVASPNHERAAKR 566
P+TPDS CQ GSP SPN R+ K+
Sbjct: 572 PMTPDSGCQVGSPADSPNGXRSTKK 646
Score = 37.3 bits (75), Expect(3) = 7e-026
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -2 / -1
Query: 76 SVEASGIYFASLYFCK*LFTWYIV 5
S E SG YFAS YFCK L TW +V
Sbjct: 181 SDEQSGKYFASRYFCKWLLTW*MV 110
Score = 31.8 bits (63), Expect(3) = 7e-026
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = -2 / -2
Query: 562 LAALSWFGLATGEPFWQLESGVSG 491
L LS GL+ G P WQ ESGV G
Sbjct: 642 LVDLSPLGLSAGXPTWQPESGVIG 571
Score = 29.5 bits (58), Expect(4) = 1e-030
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2 / +2
Query: 2 INNIPCKKSLAEIQTSKVYS* 64
+N++PC++ AEI TSK+ +*
Sbjct: 107 LNHLPCQEPFAEIPTSKILT* 169
>gb|DW019329.1|DW019329 EST1228290 MTY Medicago truncatula cDNA clone MTYBH65, mRNA
sequence
Length = 872
Score = 89.5 bits (189), Expect(3) = 3e-027
Identities = 48/107 (44%), Positives = 58/107 (54%)
Frame = -2 / -3
Query: 394 SDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDFTPVKRCCSSMIFWRYFP*ASIRXXXX 215
S +G + S N S + P G S+ TP+ RCCSS+IF++YFP*ASI
Sbjct: 750 SGSGVAGSVLLSSSCWNISPGVGADTPEPGASERTPLSRCCSSIIFFKYFP*ASILNCSC 571
Query: 214 XXXXXXXXSRFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPS 74
SRF TSI S AS IC P++PS S SP S LSV FPS
Sbjct: 570 LCTSNCSCSRFCTSI*SFSASVICIPDEPSRLESISPVSFLSVFFPS 430
Score = 68.0 bits (142), Expect(4) = 3e-030
Identities = 27/40 (67%), Positives = 35/40 (87%)
Frame = +3 / +2
Query: 66 ASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQK 185
+S +G KTD K+ GD+L+ L+GSSG+QI+EALKLQMEVQK
Sbjct: 422 SSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQK 541
Score = 57.4 bits (119), Expect(4) = 3e-030
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +3 / +2
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQF 347
AQGKYL+KIIEEQQRL+GV SE P G S D F
Sbjct: 593 AQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMF 703
Score = 41.4 bits (84), Expect(4) = 3e-030
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +3 / +2
Query: 480 SRNEPLTPDSNCQNGSPVASPN 545
S +EP+TPDS CQ GSP SPN
Sbjct: 806 SHHEPMTPDSGCQVGSPADSPN 871
Score = 37.3 bits (75), Expect(3) = 3e-027
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -2 / -3
Query: 76 SVEASGIYFASLYFCK*LFTWYIV 5
S E SG YFAS YFCK L TW +V
Sbjct: 429 SDEQSGKYFASRYFCKWLLTW*MV 358
Score = 33.6 bits (67), Expect(3) = 3e-027
Identities = 13/18 (72%), Positives = 14/18 (77%)
Frame = -2 / -3
Query: 541 GLATGEPFWQLESGVSGS 488
GL+ GEP WQ ESGV GS
Sbjct: 867 GLSAGEPTWQPESGVIGS 814
Score = 29.5 bits (58), Expect(4) = 3e-030
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2 / +1
Query: 2 INNIPCKKSLAEIQTSKVYS* 64
+N++PC++ AEI TSK+ +*
Sbjct: 355 LNHLPCQEPFAEIPTSKILT* 417
>gb|BG647218.1|BG647218 EST508837 HOGA Medicago truncatula cDNA clone pHOGA-16C19 5' end,
mRNA sequence
Length = 817
Score = 89.5 bits (189), Expect(3) = 1e-025
Identities = 48/107 (44%), Positives = 58/107 (54%)
Frame = -2 / -3
Query: 394 SDAGAGVEGSVRSESGN*SLDTVTEAPPAGVSDFTPVKRCCSSMIFWRYFP*ASIRXXXX 215
S +G + S N S + P G S+ TP+ RCCSS+IF++YFP*ASI
Sbjct: 707 SGSGVAGSVLLSSSCWNISPGVGADTPEPGASERTPLSRCCSSIIFFKYFP*ASILNCSC 528
Query: 214 XXXXXXXXSRFWTSICSLRASDICNPEDPSSPASKSPGSLLSVLFPS 74
SRF TSI S AS IC P++PS S SP S LSV FPS
Sbjct: 527 LCTSNCSCSRFCTSI*SFSASVICIPDEPSRLESISPVSFLSVFFPS 387
Score = 68.0 bits (142), Expect(4) = 2e-028
Identities = 27/40 (67%), Positives = 35/40 (87%)
Frame = +3 / +1
Query: 66 ASTDGNKTDNKDPGDLLAGLEGSSGLQISEALKLQMEVQK 185
+S +G KTD K+ GD+L+ L+GSSG+QI+EALKLQMEVQK
Sbjct: 379 SSDEGKKTDKKETGDMLSNLDGSSGMQITEALKLQMEVQK 498
Score = 57.4 bits (119), Expect(4) = 2e-028
Identities = 24/37 (64%), Positives = 26/37 (70%)
Frame = +3 / +1
Query: 237 AQGKYLQKIIEEQQRLTGVKSETPAGGASVTVSSDQF 347
AQGKYL+KIIEEQQRL+GV SE P G S D F
Sbjct: 550 AQGKYLKKIIEEQQRLSGVLSEAPGSGVSAPTPGDMF 660
Score = 37.3 bits (75), Expect(3) = 1e-025
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -2 / -3
Query: 76 SVEASGIYFASLYFCK*LFTWYIV 5
S E SG YFAS YFCK L TW +V
Sbjct: 386 SDEQSGKYFASRYFCKWLLTW*MV 315
Score = 34.5 bits (69), Expect(4) = 2e-028
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +3 / +1
Query: 480 SRNEPLTPDSNCQNGSP 530
S +EP+TPDS CQ GSP
Sbjct: 763 SHHEPMTPDSGCQVGSP 813
Score = 29.5 bits (58), Expect(4) = 2e-028
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +2 / +3
Query: 2 INNIPCKKSLAEIQTSKVYS* 64
+N++PC++ AEI TSK+ +*
Sbjct: 312 LNHLPCQEPFAEIPTSKILT* 374
Score = 28.6 bits (56), Expect(3) = 1e-025
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -2 / -3
Query: 529 GEPFWQLESGVSGS 488
GEP WQ ESGV GS
Sbjct: 812 GEPTWQPESGVIGS 771
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 551,064,811
Number of Sequences: 392609
Number of extensions: 7105911
Number of successful extensions: 53459
Number of sequences better than 1.0e-020: 7
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 17701047560
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)