TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3562100.2.1
(759 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ154720.1|BQ154720 NF099G01IR1F1008 Irradiated Medicago... 186 3e-046
gb|CA922013.1|CA922013 EST639731 MTUS Medicago truncatula c... 186 3e-046
gb|CX523674.1|CX523674 s13dNF02F03AT029_322732 Aphid-Infect... 186 3e-046
gb|BI311684.1|BI311684 EST5313434 GESD Medicago truncatula ... 179 5e-044
gb|CA990471.1|CA990471 EST643979 GESD Medicago truncatula c... 141 2e-033
gb|AC137820.11| Medicago truncatula clone mth2-15f9, comple... 85 5e-033
gb|CA990480.1|CA990480 EST643988 GESD Medicago truncatula c... 141 1e-032
gb|BF519079.1|BF519079 EST456539 DSIL Medicago truncatula c... 139 4e-032
gb|AC155101.19| Medicago truncatula clone mth2-139j13, comp... 92 5e-031
gb|BE324374.2|BE324374 NF015G01PL1F1005 Phosphate starved l... 133 4e-030
>gb|BQ154720.1|BQ154720 NF099G01IR1F1008 Irradiated Medicago truncatula cDNA clone
NF099G01IR 5', mRNA sequence
Length = 666
Score = 186 bits (401), Expect = 3e-046
Identities = 76/117 (64%), Positives = 90/117 (76%)
Frame = +3 / +1
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 226 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 405
Query: 183 LYIGDIFLVNSAEIIRPNLSIREGIEIIVSGGMTMPQVIMSLEPTPRKSQNVRLDRV 353
LYIGDI L+NS ++IRPNLSIREGIEIIVSGGMTMPQVI +E ++ + L+R+
Sbjct: 406 LYIGDIILINSKDVIRPNLSIREGIEIIVSGGMTMPQVISPIERVAHPNERISLNRI 576
Score = 118 bits (253), Expect = 8e-026
Identities = 54/87 (62%), Positives = 64/87 (73%)
Frame = -3 / -2
Query: 298 ITCGIVIPPETIISMPSRIDKLGLIISAEFTKKMSPIYK*LVGT*TLHNSSSPLSV*RTM 119
ITCGIVIPPET+IS+PSR+++ GLI S E + +SPIY+ VG TL +SSS S R
Sbjct: 521 ITCGIVIPPETMISIPSRMERFGLITSLELIRIISPIYRWFVGIKTLQSSSSSFSFCRVT 342
Query: 118 VDVMNPNAYSPTRG*LMIATSLNAXXF 38
VDVM P AYSPTRG* +IATSL A F
Sbjct: 341 VDVMKPKAYSPTRG*RIIATSLKAVVF 261
>gb|CA922013.1|CA922013 EST639731 MTUS Medicago truncatula cDNA clone MTUS-47E10, mRNA
sequence
Length = 779
Score = 186 bits (401), Expect = 3e-046
Identities = 76/117 (64%), Positives = 90/117 (76%)
Frame = +3 / -2
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 541 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 362
Query: 183 LYIGDIFLVNSAEIIRPNLSIREGIEIIVSGGMTMPQVIMSLEPTPRKSQNVRLDRV 353
LYIGDI L+NS ++IRPNLSIREGIEIIVSGGMTMPQVI +E ++ + L+R+
Sbjct: 361 LYIGDIILINSKDVIRPNLSIREGIEIIVSGGMTMPQVISPIERVAHPNERISLNRI 191
Score = 118 bits (253), Expect = 8e-026
Identities = 54/87 (62%), Positives = 64/87 (73%)
Frame = -3 / +3
Query: 298 ITCGIVIPPETIISMPSRIDKLGLIISAEFTKKMSPIYK*LVGT*TLHNSSSPLSV*RTM 119
ITCGIVIPPET+IS+PSR+++ GLI S E + +SPIY+ VG TL +SSS S R
Sbjct: 246 ITCGIVIPPETMISIPSRMERFGLITSLELIRIISPIYRWFVGIKTLQSSSSSFSFCRVT 425
Query: 118 VDVMNPNAYSPTRG*LMIATSLNAXXF 38
VDVM P AYSPTRG* +IATSL A F
Sbjct: 426 VDVMKPKAYSPTRG*RIIATSLKAVVF 506
>gb|CX523674.1|CX523674 s13dNF02F03AT029_322732 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 692
Score = 186 bits (401), Expect = 3e-046
Identities = 76/117 (64%), Positives = 90/117 (76%)
Frame = +3 / -2
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 565 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 386
Query: 183 LYIGDIFLVNSAEIIRPNLSIREGIEIIVSGGMTMPQVIMSLEPTPRKSQNVRLDRV 353
LYIGDI L+NS ++IRPNLSIREGIEIIVSGGMTMPQVI +E ++ + L+R+
Sbjct: 385 LYIGDIILINSKDVIRPNLSIREGIEIIVSGGMTMPQVISPIERVAHPNERISLNRI 215
Score = 118 bits (253), Expect = 8e-026
Identities = 54/87 (62%), Positives = 64/87 (73%)
Frame = -3 / +3
Query: 298 ITCGIVIPPETIISMPSRIDKLGLIISAEFTKKMSPIYK*LVGT*TLHNSSSPLSV*RTM 119
ITCGIVIPPET+IS+PSR+++ GLI S E + +SPIY+ VG TL +SSS S R
Sbjct: 270 ITCGIVIPPETMISIPSRMERFGLITSLELIRIISPIYRWFVGIKTLQSSSSSFSFCRVT 449
Query: 118 VDVMNPNAYSPTRG*LMIATSLNAXXF 38
VDVM P AYSPTRG* +IATSL A F
Sbjct: 450 VDVMKPKAYSPTRG*RIIATSLKAVVF 530
>gb|BI311684.1|BI311684 EST5313434 GESD Medicago truncatula cDNA clone pGESD13J1 5' end,
mRNA sequence
Length = 687
Score = 179 bits (385), Expect = 5e-044
Identities = 72/103 (69%), Positives = 85/103 (82%)
Frame = +3 / +1
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYA GFITS++VLQT GDEELC VYVPTNH
Sbjct: 145 SKQISAAISPDQNSQAFKEVAIIRHPRVGEYALGFITSSVVLQTYSGDEELCCVYVPTNH 324
Query: 183 LYIGDIFLVNSAEIIRPNLSIREGIEIIVSGGMTMPQVIMSLE 311
LYIGDIFLVN+ ++IRPNLS+REGIEI+VSGGM+MPQ++ +L+
Sbjct: 325 LYIGDIFLVNTKDVIRPNLSVREGIEIVVSGGMSMPQILSTLD 453
Score = 117 bits (251), Expect = 2e-025
Identities = 55/85 (64%), Positives = 63/85 (74%)
Frame = -3 / -2
Query: 292 CGIVIPPETIISMPSRIDKLGLIISAEFTKKMSPIYK*LVGT*TLHNSSSPLSV*RTMVD 113
CGI +PPET IS+PSR DK GL+ S T+ +SPI + VGT*T HNSSSP V*RT +
Sbjct: 434 CGIDMPPETTISIPSRTDKFGLMTSLVLTRNISPI*RWFVGT*TQHNSSSPE*V*RTTDE 255
Query: 112 VMNPNAYSPTRG*LMIATSLNAXXF 38
VMNPNAYSPTRG M+ATSL A F
Sbjct: 254 VMNPNAYSPTRGCRMMATSLKACEF 180
>gb|CA990471.1|CA990471 EST643979 GESD Medicago truncatula cDNA clone GESD-28D22, mRNA
sequence
Length = 821
Score = 141 bits (302), Expect(2) = 2e-033
Identities = 57/84 (67%), Positives = 65/84 (77%)
Frame = +3 / +2
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 482 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 661
Query: 183 LYIGDIFLVNSAEIIRPNLSIREG 254
LYIGDI L+NS ++IR NLSIREG
Sbjct: 662 LYIGDIILINSKDVIRTNLSIREG 733
Score = 22.6 bits (43), Expect(2) = 2e-033
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3 / +3
Query: 279 MTMPQVIMSLEPTPRKSQNV 338
MTMPQVI +E + ++ +
Sbjct: 756 MTMPQVISPIERSAHPNEEI 815
>gb|AC137820.11| Medicago truncatula clone mth2-15f9, complete sequence
Length = 134674
Score = 84.9 bits (179), Expect(3) = 5e-033
Identities = 32/43 (74%), Positives = 38/43 (88%)
Frame = +3 / -3
Query: 129 QTDKGDEELCSVYVPTNHLYIGDIFLVNSAEIIRPNLSIREGI 257
Q + DEELCSV++PTNHLYIGDI L+NS ++IRPNLSIREGI
Sbjct: 117650 QKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLSIREGI 117522
Score = 66.1 bits (138), Expect(3) = 5e-033
Identities = 27/33 (81%), Positives = 28/33 (84%)
Frame = +3 / -2
Query: 33 DXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQ 131
D N AFKEVAII HPRVGEYAFGFITST+ LQ
Sbjct: 118908 DQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQ 118810
Score = 47.8 bits (98), Expect(3) = 5e-033
Identities = 19/40 (47%), Positives = 27/40 (67%)
Frame = +3 / -2
Query: 234 NLSIREGIEIIVSGGMTMPQVIMSLEPTPRKSQNVRLDRV 353
N+ + G EIIVSGGMTMPQVI +E ++ + L+R+
Sbjct: 117312 NIYVDAGAEIIVSGGMTMPQVISPIERVAHPNERISLNRI 117193
>gb|CA990480.1|CA990480 EST643988 GESD Medicago truncatula cDNA clone GESD-28F22, mRNA
sequence
Length = 712
Score = 141 bits (303), Expect = 1e-032
Identities = 58/86 (67%), Positives = 66/86 (76%)
Frame = +3 / +2
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 455 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 634
Query: 183 LYIGDIFLVNSAEIIRPNLSIREGIE 260
LYIGDI L+NS ++IR NLSIRE IE
Sbjct: 635 LYIGDIILINSRDVIRTNLSIRERIE 712
>gb|BF519079.1|BF519079 EST456539 DSIL Medicago truncatula cDNA clone pDSIL-19L9, mRNA
sequence
Length = 651
Score = 139 bits (299), Expect = 4e-032
Identities = 56/82 (68%), Positives = 64/82 (78%)
Frame = +3 / +1
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITST+ LQ + DEELCSV++PTNH
Sbjct: 406 SKQISAAISPDQNTTAFKEVAIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNH 585
Query: 183 LYIGDIFLVNSAEIIRPNLSIR 248
LYIGDI L+NS ++IRPNLSIR
Sbjct: 586 LYIGDIILINSKDVIRPNLSIR 651
>gb|AC155101.19| Medicago truncatula clone mth2-139j13, complete sequence
Length = 124152
Score = 91.8 bits (194), Expect(3) = 5e-031
Identities = 36/51 (70%), Positives = 42/51 (82%)
Frame = +3 / -1
Query: 105 FITSTMVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSAEIIRPNLSIREGI 257
++ V QT GDEELC VYVPTNHLYIGDIFLVN+ ++IRPNLS+REGI
Sbjct: 113136 YLNYIAVSQTYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRPNLSVREGI 112984
Score = 86.3 bits (182), Expect(2) = 4e-027
Identities = 33/45 (73%), Positives = 39/45 (86%)
Frame = +3 / -3
Query: 123 VLQTDKGDEELCSVYVPTNHLYIGDIFLVNSAEIIRPNLSIREGI 257
+ Q+ GDEELC VYVPTNHLYIGDIFLVN+ ++IRP LS+REGI
Sbjct: 104140 LFQSYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRPTLSVREGI 104006
Score = 66.1 bits (138), Expect(2) = 4e-027
Identities = 27/36 (75%), Positives = 30/36 (83%)
Frame = +3 / -2
Query: 33 DXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDK 140
D N AFKEVAII HPRVGEYAFGFITS++VLQ +
Sbjct: 107087 DQNSQAFKEVAIIRHPRVGEYAFGFITSSVVLQVSE 106980
Score = 65.7 bits (137), Expect(3) = 5e-031
Identities = 28/40 (70%), Positives = 30/40 (75%)
Frame = +3 / -3
Query: 12 VXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQ 131
V T D N AFKEVAII HPRVGEYA GFITS++VLQ
Sbjct: 113488 VNTCAATDQNSQAFKEVAIIRHPRVGEYALGFITSSVVLQ 113369
Score = 34.5 bits (69), Expect(3) = 5e-031
Identities = 12/18 (66%), Positives = 17/18 (94%)
Frame = +3 / -1
Query: 258 EIIVSGGMTMPQVIMSLE 311
EI+VSGGM+MPQ++ +LE
Sbjct: 103536 EIVVSGGMSMPQILSTLE 103483
>gb|BE324374.2|BE324374 NF015G01PL1F1005 Phosphate starved leaf Medicago truncatula cDNA
clone NF015G01PL 5', mRNA sequence
Length = 346
Score = 133 bits (284), Expect = 4e-030
Identities = 54/77 (70%), Positives = 61/77 (79%)
Frame = +3 / +3
Query: 3 SXXVXTXXXXDXNXXAFKEVAIISHPRVGEYAFGFITSTMVLQTDKGDEELCSVYVPTNH 182
S + D N AFKEVAII HPRVGEYAFGFITS++VLQ+ GDEELC VYVPTNH
Sbjct: 111 SKQISAAISPDQNSQAFKEVAIIRHPRVGEYAFGFITSSVVLQSYSGDEELCCVYVPTNH 290
Query: 183 LYIGDIFLVNSAEIIRP 233
LYIGDIFLVN+ ++IRP
Sbjct: 291 LYIGDIFLVNTKDVIRP 341
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 749,481,856
Number of Sequences: 392609
Number of extensions: 11239374
Number of successful extensions: 48313
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 24956602110
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)