TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3438088.2.1
(615 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone m... 167 1e-041
gb|BF648440.1|BF648440 NF047E08EC1F1066 Elicited cell cultu... 167 1e-041
gb|BQ139914.1|BQ139914 NF026G11PH1F1087 Phoma-infected Medi... 167 1e-041
gb|CG934104.1|CG934104 MBENC70TR mth2 Medicago truncatula g... 156 2e-037
gb|AC144618.7| Medicago truncatula clone mth2-21d12, comple... 151 8e-036
emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-1... 151 8e-036
gb|CG959762.1|CG959762 MBEKX59TR mth2 Medicago truncatula g... 150 1e-035
gb|CG961263.1|CG961263 MBEJP43TRB mth2 Medicago truncatula ... 150 1e-035
gb|BI310382.1|BI310382 EST5312132 GESD Medicago truncatula ... 103 1e-034
gb|AC127169.13| Medicago truncatula clone mth2-36n3, comple... 130 2e-029
gb|BQ165568.1|BQ165568 EST611437 KVKC Medicago truncatula c... 105 6e-022
gb|CA922432.1|CA922432 EST640150 MTUS Medicago truncatula c... 105 6e-022
gb|AY308955.1| Medicago truncatula cellulase mRNA, complete... 105 6e-022
gb|AC150784.20| Medicago truncatula clone mth2-134j11, WORK... 105 6e-022
gb|AJ499329.1|AJ499329 AJ499329 MTGIM Medicago truncatula c... 101 8e-021
>gb|AC151000.3| Medicago truncatula chromosome 2 BAC clone mth2-193p1, complete
sequence
Length = 120579
Score = 167 bits (359), Expect(2) = 1e-041
Identities = 60/77 (77%), Positives = 65/77 (84%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 25091 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 25270
Query: 433 DRHDGFKDARRNYNYTE 383
DRHDGF D R+NYNYTE
Sbjct: 25271 DRHDGFHDVRKNYNYTE 25321
Score = 149 bits (319), Expect = 5e-035
Identities = 70/105 (66%), Positives = 74/105 (70%)
Frame = +3 / -1
Query: 300 VFLSTPPWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGF 479
VFLS SPD ATNAAT+PA PASV SV*L*F R S PS SGPATMAP + GFGF
Sbjct: 25404 VFLSIGMLLSPDNATNAATKPAFPASVGSV*L*FFRTS*KPSCLSGPATMAPTSIFGFGF 25225
Query: 480 LASLHFHPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
SLHFHPPLQ T F GI+A W TC G* P PTT*LIF+GF
Sbjct: 25224 DDSLHFHPPLQLYLTLFFGIDAALWWTCFG**LPKPTT*LIFRGF 25090
Score = 23.5 bits (45), Expect(2) = 1e-041
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +2
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 25391 IDKNTLFSAV 25420
>gb|BF648440.1|BF648440 NF047E08EC1F1066 Elicited cell culture Medicago truncatula cDNA
clone NF047E08EC 5', mRNA sequence
Length = 647
Score = 167 bits (359), Expect(2) = 1e-041
Identities = 60/77 (77%), Positives = 65/77 (84%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 146 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 325
Query: 433 DRHDGFKDARRNYNYTE 383
DRHDGF D R+NYNYTE
Sbjct: 326 DRHDGFHDVRKNYNYTE 376
Score = 149 bits (319), Expect = 5e-035
Identities = 70/105 (66%), Positives = 74/105 (70%)
Frame = +3 / -3
Query: 300 VFLSTPPWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGF 479
VFLS SPD ATNAAT+PA PASV SV*L*F R S PS SGPATMAP + GFGF
Sbjct: 459 VFLSIGMLLSPDNATNAATKPAFPASVGSV*L*FFRTS*KPSCLSGPATMAPTSIFGFGF 280
Query: 480 LASLHFHPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
SLHFHPPLQ T F GI+A W TC G* P PTT*LIF+GF
Sbjct: 279 DDSLHFHPPLQLYLTLFFGIDAALWWTCFG**LPKPTT*LIFRGF 145
Score = 23.5 bits (45), Expect(2) = 1e-041
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +2
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 446 IDKNTLFSAV 475
>gb|BQ139914.1|BQ139914 NF026G11PH1F1087 Phoma-infected Medicago truncatula cDNA clone
NF026G11PH 5', mRNA sequence
Length = 639
Score = 167 bits (359), Expect(2) = 1e-041
Identities = 60/77 (77%), Positives = 65/77 (84%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 156 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 335
Query: 433 DRHDGFKDARRNYNYTE 383
DRHDGF D R+NYNYTE
Sbjct: 336 DRHDGFHDVRKNYNYTE 386
Score = 146 bits (314), Expect = 2e-034
Identities = 69/105 (65%), Positives = 73/105 (69%)
Frame = +3 / -3
Query: 300 VFLSTPPWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGF 479
VFLS SPD A NAAT+PA PASV SV*L*F R S PS SGPATMAP + GFGF
Sbjct: 469 VFLSIGMLLSPDNAXNAATKPAFPASVGSV*L*FFRTS*KPSCLSGPATMAPTSIFGFGF 290
Query: 480 LASLHFHPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
SLHFHPPLQ T F GI+A W TC G* P PTT*LIF+GF
Sbjct: 289 DDSLHFHPPLQLYLTLFFGIDAALWWTCFG**LPKPTT*LIFRGF 155
Score = 23.5 bits (45), Expect(2) = 1e-041
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +3
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 456 IDKNTLFSAV 485
>gb|CG934104.1|CG934104 MBENC70TR mth2 Medicago truncatula genomic clone 6L19, DNA sequence
Length = 882
Score = 156 bits (336), Expect = 2e-037
Identities = 56/73 (76%), Positives = 61/73 (83%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 663 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 842
Query: 433 DRHDGFKDARRNY 395
DRHDGF D R+NY
Sbjct: 843 DRHDGFHDVRKNY 881
Score = 104 bits (221), Expect = 2e-021
Identities = 47/73 (64%), Positives = 50/73 (68%)
Frame = +3 / -3
Query: 396 *FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP**TPFLGIEAPRW*TCLG*R 575
*F R S PS SGPATMAP + GFGF SLHFHPPLQ T F GI+A W TC G*
Sbjct: 880 *FFRTS*KPSCLSGPATMAPTSIFGFGFDDSLHFHPPLQLYLTLFFGIDAALWWTCFG** 701
Query: 576 FPNPTT*LIFKGF 614
P PTT*LIF+GF
Sbjct: 700 LPKPTT*LIFRGF 662
>gb|AC144618.7| Medicago truncatula clone mth2-21d12, complete sequence
Length = 106554
Score = 151 bits (325), Expect = 8e-036
Identities = 54/110 (49%), Positives = 70/110 (63%)
Frame = -3 / -3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+ MSY+VG+G+++PRHVHHRGASIP + +Y C GGWKWRD+ NPN + GAMV GP
Sbjct: 90517 NPMNMSYIVGYGEKFPRHVHHRGASIPNDHKNYSCTGGWKWRDSPDRNPNNVTGAMVGGP 90338
Query: 433 DRHDGFKDARRNYNYTEXXXXXXXXXXXXXXXXXXXGHGGVDKNTMFSAV 284
+ D F D+R+NYNYTE G+D NT+F A+
Sbjct: 90337 NPFDQFHDSRKNYNYTEPTLAGNAGLVAALISLTSTTGSGIDVNTIFEAI 90188
>emb|CT030180.3| M.truncatula DNA sequence from clone MTH2-177C12 on chromosome 3,
complete sequence
Length = 141577
Score = 151 bits (325), Expect = 8e-036
Identities = 54/110 (49%), Positives = 70/110 (63%)
Frame = -3 / +1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+ MSY+VG+G+++PRHVHHRGASIP + +Y C GGWKWRD+ NPN + GAMV GP
Sbjct: 136090 NPMNMSYIVGYGEKFPRHVHHRGASIPNDHKNYSCTGGWKWRDSPDRNPNNVTGAMVGGP 136269
Query: 433 DRHDGFKDARRNYNYTEXXXXXXXXXXXXXXXXXXXGHGGVDKNTMFSAV 284
+ D F D+R+NYNYTE G+D NT+F A+
Sbjct: 136270 NPFDQFHDSRKNYNYTEPTLAGNAGLVAALISLTSTTGSGIDVNTIFEAI 136419
>gb|CG959762.1|CG959762 MBEKX59TR mth2 Medicago truncatula genomic clone 77J22, DNA
sequence
Length = 930
Score = 150 bits (323), Expect = 1e-035
Identities = 54/71 (76%), Positives = 59/71 (83%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 689 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 868
Query: 433 DRHDGFKDARR 401
DRHDGF D R+
Sbjct: 869 DRHDGFHDVRK 901
>gb|CG961263.1|CG961263 MBEJP43TRB mth2 Medicago truncatula genomic clone 69H14, DNA
sequence
Length = 918
Score = 150 bits (323), Expect = 1e-035
Identities = 54/71 (76%), Positives = 59/71 (83%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVGFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN + GAMVAGP
Sbjct: 689 NPRKMSYVVGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNILVGAMVAGP 868
Query: 433 DRHDGFKDARR 401
DRHDGF D R+
Sbjct: 869 DRHDGFHDVRK 901
>gb|BI310382.1|BI310382 EST5312132 GESD Medicago truncatula cDNA clone pGESD7N4 5' end,
mRNA sequence
Length = 579
Score = 103 bits (220), Expect(3) = 1e-034
Identities = 36/49 (73%), Positives = 40/49 (81%)
Frame = -3 / +1
Query: 604 KMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTI 458
KMSYV GFG YP+HVHHR ASIPKN V Y CKGGWKWR++ KPNPN +
Sbjct: 28 KMSYVDGFGNHYPKHVHHRAASIPKNKVKYNCKGGWKWRESSKPNPNIL 174
Score = 75.8 bits (159), Expect(2) = 7e-028
Identities = 37/52 (71%), Positives = 38/52 (73%)
Frame = +3 / -1
Query: 300 VFLSTPPWPSPDKATNAATRPALPASVASV*L*FLRASLNPSWRSGPATMAP 455
VFLS SPD ATNAAT+PA PASV SV*L*F R S PS SGPATMAP
Sbjct: 333 VFLSIGMLLSPDNATNAATKPAFPASVGSV*L*FFRTS*KPSCLSGPATMAP 178
Score = 69.3 bits (145), Expect(2) = 7e-028
Identities = 30/49 (61%), Positives = 34/49 (69%)
Frame = +3 / -2
Query: 462 VLGFGFLASLHFHPPLQP**TPFLGIEAPRW*TCLG*RFPNPTT*LIFK 608
+ GFGF SLHFHPPLQ T F GI+A W TC G* P P+T*LIF+
Sbjct: 170 IFGFGFDDSLHFHPPLQLYLTLFFGIDAALWWTCFG**LPKPST*LIFR 24
Score = 58.3 bits (121), Expect(3) = 1e-034
Identities = 21/24 (87%), Positives = 22/24 (91%)
Frame = -3 / +2
Query: 454 GAMVAGPDRHDGFKDARRNYNYTE 383
GAMVAGPDRHDGF D R+NYNYTE
Sbjct: 179 GAMVAGPDRHDGFHDVRKNYNYTE 250
Score = 23.5 bits (45), Expect(3) = 1e-034
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +2
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 320 IDKNTLFSAV 349
>gb|AC127169.13| Medicago truncatula clone mth2-36n3, complete sequence
Length = 124554
Score = 130 bits (279), Expect = 2e-029
Identities = 47/77 (61%), Positives = 55/77 (71%)
Frame = -3 / +3
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+ MSY+VG+G ++PRHVHHRGASIP + HY C GWKWRD NPN I GAMV GP
Sbjct: 102192 NPMNMSYIVGYGNKFPRHVHHRGASIPNDDKHYSCIEGWKWRDTPNRNPNNIVGAMVGGP 102371
Query: 433 DRHDGFKDARRNYNYTE 383
+ D F D R N +YTE
Sbjct: 102372 NGFDQFYDLRNNNSYTE 102422
>gb|BQ165568.1|BQ165568 EST611437 KVKC Medicago truncatula cDNA clone pKVKC-10B7, mRNA
sequence
Length = 781
Score = 105 bits (224), Expect = 6e-022
Identities = 40/77 (51%), Positives = 51/77 (66%)
Frame = -3 / -2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSY+VG+G ++P VHHR ASIP + Y C G W ++K PNP + GAMV GP
Sbjct: 507 NPMKMSYLVGYGDKFPVQVHHRSASIPWDKRLYNCDDGKTWLNSKNPNPQVLLGAMVGGP 328
Query: 433 DRHDGFKDARRNYNYTE 383
D +D F D R N +TE
Sbjct: 327 DTNDHFTDQRSNKRFTE 277
>gb|CA922432.1|CA922432 EST640150 MTUS Medicago truncatula cDNA clone MTUS-53E12, mRNA
sequence
Length = 735
Score = 105 bits (224), Expect = 6e-022
Identities = 40/77 (51%), Positives = 51/77 (66%)
Frame = -3 / -1
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSY+VG+G ++P VHHR ASIP + Y C G W ++K PNP + GAMV GP
Sbjct: 507 NPMKMSYLVGYGDKFPVQVHHRSASIPWDKRLYNCDDGKTWLNSKNPNPQVLLGAMVGGP 328
Query: 433 DRHDGFKDARRNYNYTE 383
D +D F D R N +TE
Sbjct: 327 DTNDHFTDQRSNKRFTE 277
>gb|AY308955.1| Medicago truncatula cellulase mRNA, complete cds
Length = 2074
Score = 105 bits (224), Expect = 6e-022
Identities = 40/77 (51%), Positives = 51/77 (66%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSY+VG+G ++P VHHR ASIP + Y C G W ++K PNP + GAMV GP
Sbjct: 1568 NPMKMSYLVGYGDKFPVQVHHRSASIPWDKRLYNCDDGKTWLNSKNPNPQVLLGAMVGGP 1747
Query: 433 DRHDGFKDARRNYNYTE 383
D +D F D R N +TE
Sbjct: 1748 DTNDHFTDQRSNKRFTE 1798
>gb|AC150784.20| Medicago truncatula clone mth2-134j11, WORKING DRAFT SEQUENCE
Length = 116850
Score = 105 bits (224), Expect = 6e-022
Identities = 40/77 (51%), Positives = 51/77 (66%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSY+VG+G ++P VHHR ASIP + Y C G W ++K PNP + GAMV GP
Sbjct: 35396 NPMKMSYLVGYGDKFPVQVHHRSASIPWDKRLYNCDDGKTWLNSKNPNPQVLLGAMVGGP 35575
Query: 433 DRHDGFKDARRNYNYTE 383
D +D F D R N +TE
Sbjct: 35576 DTNDHFTDQRSNKRFTE 35626
>gb|AJ499329.1|AJ499329 AJ499329 MTGIM Medicago truncatula cDNA clone mtgmacc120002g06,
mRNA sequence
Length = 532
Score = 101 bits (216), Expect = 8e-021
Identities = 39/77 (50%), Positives = 50/77 (64%)
Frame = -3 / -2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP+KMSY+VG+G ++ VHHR ASIP + Y C G W ++K PNP + GAMV GP
Sbjct: 495 NPMKMSYLVGYGDKFAVQVHHRSASIPWDKRLYNCDDGKTWLNSKNPNPQVLLGAMVGGP 316
Query: 433 DRHDGFKDARRNYNYTE 383
D +D F D R N +TE
Sbjct: 315 DTNDHFTDQRSNKRFTE 265
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 613,082,743
Number of Sequences: 392609
Number of extensions: 8836431
Number of successful extensions: 56549
Number of sequences better than 1.0e-020: 18
length of database: 147,244,331
effective HSP length: 53
effective length of database: 126,436,054
effective search space used: 19091844154
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)