TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204272.2.1
(739 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ155462.1|BQ155462 NF080G03IR1F1024 Irradiated Medicago... 79 2e-036
gb|CA919533.1|CA919533 EST637251 MTUS Medicago truncatula c... 78 3e-034
gb|AW773989.1|AW773989 EST332975 KV3 Medicago truncatula cD... 81 5e-032
gb|AC161401.2| Medicago truncatula chromosome 7 clone mte1-... 73 8e-026
gb|AC141107.5| Medicago truncatula clone mth2-34a12, comple... 81 9e-026
gb|BE942556.1|BE942556 EST422135 MGHG Medicago truncatula c... 81 3e-024
gb|CX521404.1|CX521404 s13dNF75F04VI032_450594 Virus-Infect... 82 8e-024
gb|BE998628.1|BE998628 EST430351 GVSN Medicago truncatula c... 81 1e-021
gb|BQ138850.1|BQ138850 NF008B12PH1F1094 Phoma-infected Medi... 81 2e-021
>gb|BQ155462.1|BQ155462 NF080G03IR1F1024 Irradiated Medicago truncatula cDNA clone
NF080G03IR 5', mRNA sequence
Length = 653
Score = 79.0 bits (166), Expect(3) = 2e-036
Identities = 31/56 (55%), Positives = 37/56 (66%)
Frame = -3 / +2
Query: 650 WNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
WNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 2 WNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 169
Score = 78.0 bits (164), Expect(3) = 2e-036
Identities = 33/64 (51%), Positives = 44/64 (68%)
Frame = -3 / +2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLALA 219
S+ + GST TVLTS ++MDENSFS P KV+P++S L + + M V + P SF+SFDL
Sbjct: 263 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDLLKE 442
Query: 218 QSKL 207
S L
Sbjct: 443 SSNL 454
Score = 35.0 bits (70), Expect(3) = 2e-036
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = -3 / +2
Query: 482 ENSFLRVKVINFGSDAVTLTISTSGL 405
+ +++R+K +NFG+ AV L IS +GL
Sbjct: 176 KKNYIRIKAVNFGTSAVNLKISFNGL 253
>gb|CA919533.1|CA919533 EST637251 MTUS Medicago truncatula cDNA clone MTUS-14H2, mRNA
sequence
Length = 668
Score = 78.0 bits (164), Expect(3) = 3e-034
Identities = 33/64 (51%), Positives = 44/64 (68%)
Frame = -3 / -2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLALA 219
S+ + GST TVLTS ++MDENSFS P KV+P++S L + + M V + P SF+SFDL
Sbjct: 340 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDLLKE 161
Query: 218 QSKL 207
S L
Sbjct: 160 SSNL 149
Score = 71.6 bits (150), Expect(3) = 3e-034
Identities = 30/57 (52%), Positives = 36/57 (63%)
Frame = -3 / -2
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
R PDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 604 R*TPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 434
Score = 35.0 bits (70), Expect(3) = 3e-034
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = -3 / -2
Query: 482 ENSFLRVKVINFGSDAVTLTISTSGL 405
+ +++R+K +NFG+ AV L IS +GL
Sbjct: 427 KKNYIRIKAVNFGTSAVNLKISFNGL 350
>gb|AW773989.1|AW773989 EST332975 KV3 Medicago truncatula cDNA clone pKV3-16D2, mRNA
sequence
Length = 616
Score = 81.3 bits (171), Expect(2) = 5e-032
Identities = 32/57 (56%), Positives = 38/57 (66%)
Frame = -3 / -2
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 552 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 382
Score = 78.0 bits (164), Expect(2) = 5e-032
Identities = 33/64 (51%), Positives = 44/64 (68%)
Frame = -3 / -2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKVVPVKSDLSNAAEQMQVTLAPRSFSSFDLALA 219
S+ + GST TVLTS ++MDENSFS P KV+P++S L + + M V + P SF+SFDL
Sbjct: 288 SLQSSGSTKTVLTSTNLMDENSFSQPKKVIPIQSLLQSVGKDMNVIVPPHSFTSFDLLKE 109
Query: 218 QSKL 207
S L
Sbjct: 108 SSNL 97
>gb|AC161401.2| Medicago truncatula chromosome 7 clone mte1-59b16, *** SEQUENCING IN
PROGRESS ***, 2 ordered pieces
Length = 95675
Score = 73.5 bits (154), Expect(4) = 8e-026
Identities = 28/41 (68%), Positives = 34/41 (82%)
Frame = -3 / +2
Query: 659 KNRWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTI 537
+NRW PDAIVFNS+Q+YGTPSYW+Q LF ESSGA +T+
Sbjct: 7916 QNRWIPDAIVFNSYQNYGTPSYWLQQLFIESSGATLLNSTL 8038
Score = 73.5 bits (154), Expect(4) = 8e-026
Identities = 28/41 (68%), Positives = 34/41 (82%)
Frame = -3 / +3
Query: 659 KNRWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTI 537
+NRW PDAIVFNS+Q+YGTPSYW+Q LF ESSGA +T+
Sbjct: 1419 QNRWIPDAIVFNSYQNYGTPSYWLQQLFIESSGATLLNSTL 1541
Score = 55.1 bits (114), Expect(4) = 8e-026
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = -3 / +1
Query: 464 VKVINFGSDAVTLTISTSGLQASINALGSTATVLTSADVMDENSFSNPTKV 312
++V+NFG+ IS +GL + + + S+ VLTS++ MDENSFS P KV
Sbjct: 8251 LQVVNFGNATENFMISINGLNSIVQSSNSSMVVLTSSNRMDENSFSEPNKV 8403
Score = 55.1 bits (114), Expect(4) = 8e-026
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = -3 / +2
Query: 464 VKVINFGSDAVTLTISTSGLQASINALGSTATVLTSADVMDENSFSNPTKV 312
++V+NFG+ IS +GL + + + S+ VLTS++ MDENSFS P KV
Sbjct: 1754 LQVVNFGNATENFMISINGLNSIVQSSNSSMVVLTSSNRMDENSFSEPNKV 1906
Score = 35.9 bits (72), Expect(4) = 8e-026
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -3 / +1
Query: 317 KVVPVKSDLSNAAEQMQVTLAPRSFSSFDLAL 222
++VP ++ L NA+ M V L P S +SFDL++
Sbjct: 47914 QIVPQRTALGNASNDMDVRLPPYSVTSFDLSI 48009
Score = 35.9 bits (72), Expect(4) = 8e-026
Identities = 14/32 (43%), Positives = 22/32 (68%)
Frame = -3 / +3
Query: 317 KVVPVKSDLSNAAEQMQVTLAPRSFSSFDLAL 222
++VP ++ L NA+ M V L P S +SFDL++
Sbjct: 2130 QIVPQRTALGNASNDMDVRLPPYSVTSFDLSI 2225
Score = 33.1 bits (66), Expect(4) = 8e-026
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3 / +2
Query: 737 AVWRGDAGRGXXXXXXXXXXXLTGLEKNRWN 645
AVW+ DAG G L GLEKNR++
Sbjct: 7523 AVWKEDAGAGSLYAAVAEAAFLIGLEKNRFD 7615
Score = 33.1 bits (66), Expect(4) = 8e-026
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3 / +3
Query: 737 AVWRGDAGRGXXXXXXXXXXXLTGLEKNRWN 645
AVW+ DAG G L GLEKNR++
Sbjct: 1026 AVWKEDAGAGSLYAAVAEAAFLIGLEKNRFD 1118
>gb|AC141107.5| Medicago truncatula clone mth2-34a12, complete sequence
Length = 142659
Score = 81.3 bits (171), Expect(4) = 9e-026
Identities = 32/57 (56%), Positives = 38/57 (66%)
Frame = -3 / +2
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 58688 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 58858
Score = 46.0 bits (94), Expect(4) = 9e-026
Identities = 19/29 (65%), Positives = 23/29 (79%)
Frame = -3 / +2
Query: 398 SINALGSTATVLTSADVMDENSFSNPTKV 312
S+ + GST TVLTS ++MDENSFS P KV
Sbjct: 59318 SLQSSGSTKTVLTSTNLMDENSFSQPKKV 59404
Score = 37.3 bits (75), Expect(4) = 9e-026
Identities = 15/37 (40%), Positives = 23/37 (62%)
Frame = -3 / +1
Query: 317 KVVPVKSDLSNAAEQMQVTLAPRSFSSFDLALAQSKL 207
+V+P++S L + + M V + P SF+SFDL S L
Sbjct: 59536 QVIPIQSLLQSVGKDMNVIVPPHSFTSFDLLKESSNL 59646
Score = 34.5 bits (69), Expect(4) = 9e-026
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +3
Query: 735 CLER*CR*RKSSCFIGRSCFPYWTGEE 655
C +*CR RKS RS PYWTG+E
Sbjct: 58080 CNRK*CRARKSFGSPCRSWIPYWTGKE 58160
>gb|BE942556.1|BE942556 EST422135 MGHG Medicago truncatula cDNA clone pMGHG-8J4, mRNA
sequence
Length = 624
Score = 81.3 bits (171), Expect(3) = 3e-024
Identities = 32/57 (56%), Positives = 38/57 (66%)
Frame = -3 / +2
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 347 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 517
Score = 35.0 bits (70), Expect(3) = 3e-024
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = -3 / +2
Query: 482 ENSFLRVKVINFGSDAVTLTISTSGL 405
+ +++R+K +NFG+ AV L IS +GL
Sbjct: 524 KKNYIRIKAVNFGTSAVNLKISFNGL 601
Score = 34.5 bits (69), Expect(3) = 3e-024
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +1
Query: 735 CLER*CR*RKSSCFIGRSCFPYWTGEE 655
C +*CR RKS RS PYWTG+E
Sbjct: 208 CNRK*CRARKSFGSPCRSWIPYWTGKE 288
>gb|CX521404.1|CX521404 s13dNF75F04VI032_450594 Virus-Infected Leaves Medicago truncatula
cDNA, mRNA sequence
Length = 455
Score = 81.7 bits (172), Expect(3) = 8e-024
Identities = 32/66 (48%), Positives = 42/66 (63%)
Frame = -3 / +3
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDSENS 474
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S +
Sbjct: 168 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNSVDK 347
Query: 473 FLRVKV 456
+++
Sbjct: 348 KNHIRI 365
Score = 34.5 bits (69), Expect(3) = 8e-024
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +2
Query: 735 CLER*CR*RKSSCFIGRSCFPYWTGEE 655
C +*CR RKS RS PYWTG+E
Sbjct: 29 CNRK*CRARKSFGSPCRSWIPYWTGKE 109
Score = 33.1 bits (66), Expect(3) = 8e-024
Identities = 12/26 (46%), Positives = 19/26 (73%)
Frame = -3 / +3
Query: 482 ENSFLRVKVINFGSDAVTLTISTSGL 405
+ + +R+K +NFG+ AV L IS +GL
Sbjct: 345 KKNHIRIKAVNFGTSAVNLKISFNGL 422
>gb|BE998628.1|BE998628 EST430351 GVSN Medicago truncatula cDNA clone pGVSN-12F5, mRNA
sequence
Length = 580
Score = 81.3 bits (171), Expect(3) = 1e-021
Identities = 32/57 (56%), Positives = 38/57 (66%)
Frame = -3 / +1
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 349 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 519
Score = 34.5 bits (69), Expect(3) = 1e-021
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +3
Query: 735 CLER*CR*RKSSCFIGRSCFPYWTGEE 655
C +*CR RKS RS PYWTG+E
Sbjct: 210 CNRK*CRARKSFGSPCRSWIPYWTGKE 290
Score = 25.8 bits (50), Expect(3) = 1e-021
Identities = 7/17 (41%), Positives = 14/17 (82%)
Frame = -3 / +1
Query: 482 ENSFLRVKVINFGSDAV 432
+ +++R+K +NFG+ AV
Sbjct: 526 KKNYIRIKAVNFGTSAV 576
>gb|BQ138850.1|BQ138850 NF008B12PH1F1094 Phoma-infected Medicago truncatula cDNA clone
NF008B12PH 5', mRNA sequence
Length = 670
Score = 81.3 bits (171), Expect(3) = 2e-021
Identities = 32/57 (56%), Positives = 38/57 (66%)
Frame = -3 / +3
Query: 653 RWNPDAIVFNSWQHYGTPSYWMQTLFRESSGAMFHPTTIXXXXXXXXXXXAITWQDS 483
RWNPDAIVFNS+Q YGTPSYWMQ F ES+GA +++ AITWQ+S
Sbjct: 435 RWNPDAIVFNSFQLYGTPSYWMQLFFSESNGATLLNSSLQTTASNSLVASAITWQNS 605
Score = 34.5 bits (69), Expect(3) = 2e-021
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = -2 / +2
Query: 735 CLER*CR*RKSSCFIGRSCFPYWTGEE 655
C +*CR RKS RS PYWTG+E
Sbjct: 296 CNRK*CRARKSFGSPCRSWIPYWTGKE 376
Score = 25.4 bits (49), Expect(3) = 2e-021
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = -3 / +2
Query: 476 SFLRVKVINFGSDAVTL 426
+++R K +NFG+ AV L
Sbjct: 617 NYIRXKAVNFGTSAVNL 667
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 723,517,696
Number of Sequences: 392609
Number of extensions: 9844832
Number of successful extensions: 42919
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 24074297995
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)