TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594433.2.1
(766 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA921322.1|CA921322 EST639040 MTUS Medicago truncatula c... 163 6e-063
gb|CA858045.1|CA858045 EST635300 GLSD Medicago truncatula c... 163 6e-063
gb|BQ124593.1|BQ124593 EST610169 GLSD Medicago truncatula c... 156 4e-037
gb|CB891913.1|CB891913 EST648882 KV3 Medicago truncatula cD... 100 2e-028
>gb|CA921322.1|CA921322 EST639040 MTUS Medicago truncatula cDNA clone MTUS-39A2, mRNA
sequence
Length = 818
Score = 163 bits (350), Expect(2) = 6e-063
Identities = 59/87 (67%), Positives = 73/87 (83%)
Frame = -1 / -1
Query: 736 LKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSV 557
+K DQ MWYRACKTCNKKVTE +GYWCE CQKND+EC+ RYIMV KVSD +GEA+VS
Sbjct: 596 IKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAKVSDASGEAFVST 417
Query: 556 FNEHAEKIIGCSADELDRIRKEEGDDS 476
FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 416 FNEEAEKIIGCPAEELDNLKSQDGEEN 336
Score = 110 bits (235), Expect(2) = 2e-034
Identities = 47/72 (65%), Positives = 51/72 (70%)
Frame = +3 / +1
Query: 528 PMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSHQYPDPKVSVTFLLQVLQA 707
PMIFSA SL ET ASP+ SET T++YL HS SFFW SHQYP SVTFLLQVLQA
Sbjct: 388 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 567
Query: 708 RYHMFWSGLXGL 743
RYH+ WS L
Sbjct: 568 RYHIAWSAFMKL 603
Score = 99.6 bits (211), Expect(2) = 6e-063
Identities = 38/61 (62%), Positives = 51/61 (83%)
Frame = -1 / -1
Query: 484 DDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLT 305
++ Y +KLK+ATW+PHL RVSV+Q+EYN EKRQRIT R+ P++ AAESK+LLE I+K+
Sbjct: 341 ENPYDMKLKKATWIPHLLRVSVSQNEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 162
Query: 304 A 302
A
Sbjct: 161 A 159
Score = 57.0 bits (118), Expect(2) = 2e-034
Identities = 31/57 (54%), Positives = 35/57 (61%)
Frame = +3 / +1
Query: 297 *QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSF 467
*+A F + S R LDSAA S G + VILCLFS LYS TLTR R G VA +F
Sbjct: 154 *EATIFDMSSRRNLDSAAKSIGTTARAVILCLFSPLYSF*ETLTRKRCGIHVAFFNF 324
>gb|CA858045.1|CA858045 EST635300 GLSD Medicago truncatula cDNA clone pGLSD-26H18, mRNA
sequence
Length = 813
Score = 163 bits (350), Expect(2) = 6e-063
Identities = 59/87 (67%), Positives = 73/87 (83%)
Frame = -1 / +2
Query: 736 LKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSV 557
+K DQ MWYRACKTCNKKVTE +GYWCE CQKND+EC+ RYIMV KVSD +GEA+VS
Sbjct: 206 IKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAKVSDASGEAFVST 385
Query: 556 FNEHAEKIIGCSADELDRIRKEEGDDS 476
FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 386 FNEEAEKIIGCPAEELDNLKSQDGEEN 466
Score = 110 bits (235), Expect(2) = 2e-034
Identities = 47/72 (65%), Positives = 51/72 (70%)
Frame = +3 / -1
Query: 528 PMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSHQYPDPKVSVTFLLQVLQA 707
PMIFSA SL ET ASP+ SET T++YL HS SFFW SHQYP SVTFLLQVLQA
Sbjct: 414 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 235
Query: 708 RYHMFWSGLXGL 743
RYH+ WS L
Sbjct: 234 RYHIAWSAFMKL 199
Score = 99.6 bits (211), Expect(2) = 6e-063
Identities = 38/61 (62%), Positives = 51/61 (83%)
Frame = -1 / +2
Query: 484 DDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLT 305
++ Y +KLK+ATW+PHL RVSV+Q+EYN EKRQRIT R+ P++ AAESK+LLE I+K+
Sbjct: 461 ENPYDMKLKKATWIPHLLRVSVSQNEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 640
Query: 304 A 302
A
Sbjct: 641 A 643
Score = 57.0 bits (118), Expect(2) = 2e-034
Identities = 31/57 (54%), Positives = 35/57 (61%)
Frame = +3 / -1
Query: 297 *QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSF 467
*+A F + S R LDSAA S G + VILCLFS LYS TLTR R G VA +F
Sbjct: 648 *EATIFDMSSRRNLDSAAKSIGTTARAVILCLFSPLYSF*ETLTRKRCGIHVAFFNF 478
>gb|BQ124593.1|BQ124593 EST610169 GLSD Medicago truncatula cDNA clone pGLSD-35P17, mRNA
sequence
Length = 639
Score = 156 bits (335), Expect = 4e-037
Identities = 58/80 (72%), Positives = 67/80 (83%)
Frame = -1 / +3
Query: 736 LKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSV 557
+K DQ MWYRACKTCNKKVTE +GYWCE CQKND+EC+ RYIMV KVSD +GEA+VS
Sbjct: 399 IKADQAMWYRACKTCNKKVTEDMDAGYWCESCQKNDAECNFRYIMVAKVSDASGEAFVST 578
Query: 556 FNEHAEKIIGCSADELDRIR 497
FNE AEKIIGC A+ELD ++
Sbjct: 579 FNEEAEKIIGCPAEELDNLK 638
Score = 110 bits (235), Expect = 3e-023
Identities = 47/72 (65%), Positives = 51/72 (70%)
Frame = +3 / -3
Query: 528 PMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSHQYPDPKVSVTFLLQVLQA 707
PMIFSA SL ET ASP+ SET T++YL HS SFFW SHQYP SVTFLLQVLQA
Sbjct: 607 PMIFSASSLKVETKASPLASETFATIIYLKLHSASFFWQLSHQYPASMSSVTFLLQVLQA 428
Query: 708 RYHMFWSGLXGL 743
RYH+ WS L
Sbjct: 427 RYHIAWSAFMKL 392
>gb|CB891913.1|CB891913 EST648882 KV3 Medicago truncatula cDNA clone KV3-52F13, mRNA
sequence
Length = 370
Score = 100 bits (212), Expect(2) = 2e-028
Identities = 38/61 (62%), Positives = 51/61 (83%)
Frame = -1 / -1
Query: 484 DDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLT 305
++ Y +KLK+ATW+PHL RVSV+Q+EYN EKRQRIT R+ P++ AAESK+LLE I+K+
Sbjct: 289 ENPYDMKLKKATWIPHLLRVSVSQYEYNGEKRQRITARAVVPIDFAAESKFLLEDISKMV 110
Query: 304 A 302
A
Sbjct: 109 A 107
Score = 47.3 bits (97), Expect(2) = 2e-028
Identities = 16/29 (55%), Positives = 24/29 (82%)
Frame = -1 / -1
Query: 562 SVFNEHAEKIIGCSADELDRIRKEEGDDS 476
S FNE AEKIIGC A+ELD ++ ++G+++
Sbjct: 370 STFNEEAEKIIGCPAEELDNLKSQDGEEN 284
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 730,992,889
Number of Sequences: 392609
Number of extensions: 9612192
Number of successful extensions: 45191
Number of sequences better than 1.0e-020: 4
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 25208689000
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)