TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521825.2.1
(2812 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC170988.4| Medicago truncatula chromosome 7 clone mth2-... 93 e-140
gb|BI308555.1|BI308555 EST529965 GPOD Medicago truncatula c... 139 9e-065
gb|AC157377.1| Medicago truncatula chromosome 7 clone mth2-... 84 5e-035
gb|CX530044.1|CX530044 s13dNF98E02MJ008_246124 Methyl Jasmo... 131 7e-029
>gb|AC170988.4| Medicago truncatula chromosome 7 clone mth2-53m12, *** SEQUENCING IN
PROGRESS ***
Length = 122501
Score = 93.2 bits (197), Expect(13) = e-140
Identities = 34/52 (65%), Positives = 42/52 (80%)
Frame = -2 / +1
Query: 1290 YLKNKDDSEWSMGEIAHTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDK 1135
+L K D +W MG+I HTLTNRR+ E C+ YAESHDQ++VGDKT+AF LMDK
Sbjct: 112399 FLCRKKDEDWRMGDIVHTLTNRRWLENCVVYAESHDQALVGDKTLAFWLMDK 112554
Score = 86.3 bits (182), Expect(13) = e-140
Identities = 34/50 (68%), Positives = 41/50 (82%)
Frame = -2 / +1
Query: 1137 KEMYTGMSDLQPASPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFGHP 988
++MY M+ +P++P IDRGIAL KMI ITM LGG+GYLNFMGNEFGHP
Sbjct: 112936 QDMYDFMALDRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHP 113085
Score = 79.9 bits (168), Expect(13) = e-140
Identities = 29/38 (76%), Positives = 34/38 (89%)
Frame = -2 / +1
Query: 1626 VLRFLLSNLRYWLDEFMFDGFRFDGVTSMLYHHHGINV 1513
VLR+LLSN R+WLDE+ FDGFRFDGVTSM+Y HHG+ V
Sbjct: 103495 VLRYLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLQV 103608
Score = 78.5 bits (165), Expect(13) = e-140
Identities = 31/41 (75%), Positives = 35/41 (85%)
Frame = -2 / +1
Query: 1890 FGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVH 1768
+ YHVTNFFA SSR GTPEDLK L+D+AH LGL VLMD+VH
Sbjct: 102418 YRYHVTNFFAPSSRFGTPEDLKSLIDRAHELGLLVLMDIVH 102540
Score = 74.4 bits (156), Expect(13) = e-140
Identities = 29/53 (54%), Positives = 38/53 (71%)
Frame = -2 / +1
Query: 840 FLSSSKQIVSDMNDEEKVIVFERGDLVFVFNFHPKKTYEGYKVGCDLPGKYRV 682
F+ S Q +S N+ ++VI+FER +LVFVFNFH +Y YKVGC PGKY+V
Sbjct: 116098 FMISEHQYISRKNEGDRVIIFERDNLVFVFNFHWTNSYSDYKVGCLKPGKYKV 116256
Score = 69.3 bits (145), Expect(13) = e-140
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = -2 / +1
Query: 2010 EKPAVSTYREFADNVLPRIRANNYNTVQLMAVMEHSYYASFG 1885
++P ++TY F D+VLPRI+ YN VQ+MA+ EHSYYASFG
Sbjct: 102100 QEPKINTYANFRDDVLPRIKKLGYNAVQIMAIQEHSYYASFG 102225
Score = 68.0 bits (142), Expect(13) = e-140
Identities = 27/46 (58%), Positives = 32/46 (69%)
Frame = -2 / +2
Query: 1521 INVGFTGNYQEYFSLDTAVDAVVYMMLANHLMHKLLPEATVVAEDV 1384
+ V FTGNY EYF T V+AVVY+ML N L+H L PEA + EDV
Sbjct: 110699 LQVSFTGNYNEYFGFATDVEAVVYLMLVNDLIHGLYPEAVSIGEDV 110836
Score = 65.7 bits (137), Expect(13) = e-140
Identities = 23/50 (46%), Positives = 33/50 (66%)
Frame = -2 / +3
Query: 1773 VHSHASNNVTDGLNGYDVGQSTQESYFHAGDRGYHKLWDSRLFNYANWEV 1624
+ S+ ++ L+G ++ T YFH+G RGYH +WDSRLFNY +WEV
Sbjct: 103245 ISDDCSHASSNTLDGLNMFDGTDAHYFHSGSRGYHWMWDSRLFNYGSWEV 103394
Score = 63.4 bits (132), Expect(13) = e-140
Identities = 22/35 (62%), Positives = 27/35 (77%)
Frame = -2 / +3
Query: 1386 VSGMPVLCRPVDEGGVGFDYRLAMAIPDRWIDYLK 1282
VSGMP C P +GG+GF+YRL MA+ D+WID LK
Sbjct: 111024 VSGMPTFCLPTQDGGIGFNYRLHMAVADKWIDLLK 111128
Score = 59.3 bits (123), Expect(9) = 4e-044
Identities = 27/41 (65%), Positives = 30/41 (73%)
Frame = +2 / -1
Query: 1763 WLWTTSIRTRKPKLCALSTRYLRSSGVPDLLLTAKKFVTWY 1885
+L T SIRT +P ALS R LRSSGVP+ LL AKK VTWY
Sbjct: 102545 YLCTISIRTSRPSSWALSIRDLRSSGVPNRLLGAKKLVTWY 102423
Score = 51.5 bits (106), Expect(13) = e-140
Identities = 18/26 (69%), Positives = 22/26 (84%)
Frame = -2 / +1
Query: 2091 ERYTFKHPRPSKPAAPRIYEAHVGMS 2014
E+Y FKHP+P +P + RIYEAHVGMS
Sbjct: 101542 EKYVFKHPQPKRPQSIRIYEAHVGMS 101619
Score = 47.3 bits (97), Expect(9) = 4e-044
Identities = 23/42 (54%), Positives = 27/42 (64%)
Frame = +2 / -1
Query: 680 ATLYFPGKSHPTL*PS*VFLGWKLKTKTKSPRSKTITFSSSF 805
+TLYFPG PTL* *+ + KL TKT+ SK IT S SF
Sbjct: 116258 STLYFPGFKQPTL*SE*LLVQ*KLNTKTRLSLSKIITLSPSF 116133
Score = 46.9 bits (96), Expect(9) = 4e-044
Identities = 20/36 (55%), Positives = 23/36 (63%)
Frame = +2 / -2
Query: 1280 FFR*SIHLSGIAIARR*SNPTPPSSTGRQRTGMPET 1387
+ R SIHLS AI +R* P PPS GRQ G+P T
Sbjct: 111130 YLRRSIHLSATAICKR*LKPIPPSCVGRQNVGIPLT 111023
Score = 46.4 bits (95), Expect(9) = 4e-044
Identities = 22/38 (57%), Positives = 25/38 (65%)
Frame = +2 / -1
Query: 1514 TLIPW**YSIDVTPSNRKPSNMNSSNQYLRLERRNLNT 1627
T P * Y IDVTPSNR PSN+ SS+ +L E R L T
Sbjct: 103607 TCSP**VYIIDVTPSNRNPSNLYSSSHHLAFESRYLRT 103494
Score = 45.1 bits (92), Expect(9) = 4e-044
Identities = 25/44 (56%), Positives = 29/44 (65%)
Frame = +2 / -3
Query: 1385 TSSATTVASGKSLCIKWFASIM*TTASTAVSKLKYSW*FPVNPT 1516
TSS ASG S ++ SI *TTAST+V+K KYS *FPV T
Sbjct: 110835 TSSPIETASG*SP*MRSLTSIR*TTASTSVAKPKYSL*FPVKDT 110704
Score = 45.1 bits (92), Expect(9) = 4e-044
Identities = 20/48 (41%), Positives = 25/48 (52%)
Frame = +1 / -3
Query: 1135 LVHQEKCNSFVANNRLIMALSICDAFFSISPVSQSMRYFTHRPLRVIF 1278
LVHQ KC V N L+M SI F V Q + Y TH P+ ++F
Sbjct: 112554 LVHQPKC*CLVTNQGLVMTFSINHTIF*PPSVCQCVDYITHSPIFILF 112411
Score = 38.2 bits (77), Expect(9) = 4e-044
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +1 / -3
Query: 1882 VPERSIVRMLHNCHQLNCVVVICSYAWQHIVCKFPICAYCWL 2007
+P+ SIVRM + H L+ ++ A Q+I+ K IC WL
Sbjct: 102228 LPKASIVRMFLDSHNLDSIIAKLFNARQYIISKIGICVDFWL 102103
Score = 35.0 bits (70), Expect(9) = 4e-044
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +1 / -3
Query: 991 VTKLISHKIQVAITSKGHCDEVNHLLECNPSINCR 1095
+ + I HKI + T+K H ++ NH + CN +I+ R
Sbjct: 113082 MAEFIPHKI*IPFTTKAHSNKPNHFV*CNTTID*R 112978
Score = 32.7 bits (65), Expect(13) = e-140
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2 / +2
Query: 915 DTDHLRYKYMNAFDQAMNALDERF 844
D ++LRY M FD+AM L+ER+
Sbjct: 115661 DAEYLRYHGMQEFDRAMQHLEERY 115732
Score = 32.7 bits (65), Expect(13) = e-140
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -2 / +2
Query: 963 GNNWSYDKCRRQWSLV 916
GNN SYDKCRR++ LV
Sbjct: 113279 GNNNSYDKCRRRFDLV 113326
Score = 32.2 bits (64), Expect(9) = 4e-044
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = +1 / -1
Query: 1624 YLPVSIVEQPTIPKFMITSISRMKIGLLGALSN 1722
Y P SIV++ IP MIT +RM+I +G++ +
Sbjct: 103394 YFPASIVKKARIPHPMITP*TRMEIMSIGSIKH 103296
Score = 29.0 bits (57), Expect(13) = e-140
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = -2 / +3
Query: 2355 QEAELIGDFNDWN 2317
Q A L+GDFN+WN
Sbjct: 98364 QSAALVGDFNNWN 98402
>gb|BI308555.1|BI308555 EST529965 GPOD Medicago truncatula cDNA clone pGPOD-7O17 5' end,
mRNA sequence
Length = 689
Score = 139 bits (298), Expect(2) = 9e-065
Identities = 55/99 (55%), Positives = 72/99 (72%)
Frame = -2 / +3
Query: 963 GNNWSYDKCRRQWSLVDTDHLRYKYMNAFDQAMNALDERFSFLSSSKQIVSDMNDEEKVI 784
GNN SYDKCRR++ L D ++LRY M FD+AM L+ER+ F+ S Q +S N+ ++VI
Sbjct: 246 GNNNSYDKCRRRFDLGDAEYLRYHGMQEFDRAMQHLEERYGFMISEHQYISRKNEGDRVI 425
Query: 783 VFERGDLVFVFNFHPKKTYEGYKVGCDLPGKYRVALDSD 667
+FER +LVFVFNFH +Y YKVGC PGKY++ LDSD
Sbjct: 426 IFERDNLVFVFNFHWTNSYSDYKVGCLKPGKYKIVLDSD 542
Score = 131 bits (281), Expect(2) = 9e-065
Identities = 52/69 (75%), Positives = 59/69 (85%)
Frame = -2 / +3
Query: 1173 VGDKTIAFLLMDKEMYTGMSDLQPASPTIDRGIALQKMIHFITMALGGDGYLNFMGNEFG 994
VGDKT+AF LMDK+MY M+ +P++P IDRGIAL KMI ITM LGG+GYLNFMGNEFG
Sbjct: 3 VGDKTLAFWLMDKDMYDFMALDRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFG 182
Query: 993 HPEWIDFPR 967
HPEWIDFPR
Sbjct: 183 HPEWIDFPR 209
Score = 84.9 bits (179), Expect(3) = 5e-026
Identities = 52/118 (44%), Positives = 65/118 (55%)
Frame = +2 / -2
Query: 611 DVK*STSWPTLPCPPKTRASESRATLYFPGKSHPTL*PS*VFLGWKLKTKTKSPRSKTIT 790
+VK S +L PP +SES+ LYFPG PTL* *+ + KL TKT+ SK IT
Sbjct: 598 EVKYSAV*LSLLKPPNKDSSESKTILYFPGFKQPTL*SE*LLVQ*KLNTKTRLSLSKIIT 419
Query: 791 FSSSFMSLTICFDDERKENLSSSAFIAWSNAFMYLYRK*SVSTRLHCRLHLS*LQLFP 964
S SF + K LSS IA SN+ + Y + S S + + RLHLS*L LFP
Sbjct: 418 LSPSFFLEIYWCSEIMKPYLSSRCCIARSNSCIP*YLRYSASPKSNLRLHLS*LLLFP 245
Score = 46.0 bits (94), Expect(3) = 5e-026
Identities = 16/42 (38%), Positives = 27/42 (64%)
Frame = +1 / -1
Query: 970 WKVNPFWVTKLISHKIQVAITSKGHCDEVNHLLECNPSINCR 1095
WK++P + + I HKI + T+K H ++ NH + CN +I+ R
Sbjct: 206 WKIDPLRMAEFIPHKI*IPFTTKAHSNKPNHFV*CNTTID*R 81
Score = 28.1 bits (55), Expect(3) = 5e-026
Identities = 10/19 (52%), Positives = 11/19 (57%)
Frame = +1 / -1
Query: 1117 HASVHFLVHQEKCNSFVAN 1173
H +H LVHQ KC V N
Sbjct: 59 HEVIHILVHQPKC*CLVTN 3
>gb|AC157377.1| Medicago truncatula chromosome 7 clone mth2-75b23, *** SEQUENCING IN
PROGRESS ***, 53 unordered pieces
Length = 201327
Score = 84.0 bits (177), Expect(3) = 5e-035
Identities = 33/77 (42%), Positives = 50/77 (64%)
Frame = -2 / +2
Query: 1992 TYREFADNVLPRIRANNYNTVQLMAVMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVD 1813
++RE A+ + + AN + V+ M VMEH +Y S+GY T +FA +SR G+PE+L L+D
Sbjct: 11897 SWRELAEQLPDYLAANGFTHVEFMPVMEHPFYGSWGYQTTGYFAPTSRYGSPEELMCLID 12076
Query: 1812 KAHSLGLRVLMDVVHSH 1762
H G+ V++D V SH
Sbjct: 12077 ALHRRGVGVILDWVPSH 12127
Score = 67.5 bits (141), Expect(3) = 5e-035
Identities = 26/51 (50%), Positives = 32/51 (62%)
Frame = -2 / +2
Query: 1686 GDRGYHKLWDSRLFNYANWEVLRFLLSNLRYWLDEFMFDGFRFDGVTSMLY 1534
G RG+H W S +FNY EV FL+S+ YWL+ + DG R D V SMLY
Sbjct: 12191 GRRGFHPDWKSAIFNYGRSEVRCFLISSAHYWLERYHADGIRVDAVASMLY 12343
Score = 37.7 bits (76), Expect(3) = 5e-035
Identities = 16/61 (26%), Positives = 34/61 (55%)
Frame = -2 / +2
Query: 1467 VDAVVYMMLANHLMHKLLPEATVVAEDVSGMPVLCRPVDEGGVGFDYRLAMAIPDRWIDY 1288
++A+ ++ N +++ +V AE+ + P + +PV GG+GF Y+ M + +I Y
Sbjct: 12404 LEAIDFLHELNSALYEDFEGISVTAEESTSWPAVTKPVWLGGLGFGYKWNMGWMNDFISY 12583
Query: 1287 L 1285
+
Sbjct: 12584 I 12586
>gb|CX530044.1|CX530044 s13dNF98E02MJ008_246124 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 573
Score = 131 bits (280), Expect = 7e-029
Identities = 51/94 (54%), Positives = 68/94 (72%)
Frame = -2 / +3
Query: 948 YDKCRRQWSLVDTDHLRYKYMNAFDQAMNALDERFSFLSSSKQIVSDMNDEEKVIVFERG 769
YDKCRR++ L D ++LRY M FD+AM L+ER+ F+ S Q +S N+ ++VI+FER
Sbjct: 12 YDKCRRRFDLGDAEYLRYHGMQEFDRAMQHLEERYGFMISEHQYISRKNEGDRVIIFERD 191
Query: 768 DLVFVFNFHPKKTYEGYKVGCDLPGKYRVALDSD 667
+LVFVFNFH +Y YKVGC PGKY++ LDSD
Sbjct: 192 NLVFVFNFHWTNSYSDYKVGCLKPGKYKIVLDSD 293
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,868,110,341
Number of Sequences: 392609
Number of extensions: 43448166
Number of successful extensions: 165295
Number of sequences better than 1.0e-020: 6
length of database: 147,244,331
effective HSP length: 58
effective length of database: 124,473,009
effective search space used: 109287301902
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)