TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478075.2.1
(807 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC146562.17| Medicago truncatula clone mth2-14d10, compl... 100 6e-025
gb|AC135318.40| Medicago truncatula clone mth2-36o3, WORKIN... 67 4e-024
gb|AC149129.3| Medicago truncatula chromosome 7 BAC clone m... 67 4e-024
gb|BE239884.1|BE239884 EST403933 MHRP- Medicago truncatula ... 74 3e-021
gb|CA921296.1|CA921296 EST639014 MTUS Medicago truncatula c... 74 3e-021
gb|AJ845937.1|AJ845937 AJ845937 MtSNF Medicago truncatula c... 74 4e-021
>gb|AC146562.17| Medicago truncatula clone mth2-14d10, complete sequence
Length = 118505
Score = 100 bits (214), Expect(2) = 6e-025
Identities = 39/82 (47%), Positives = 53/82 (64%)
Frame = +3 / +2
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA++D+ +AA+EK G +++VVSE+GWPS GG+ G
Sbjct: 26954 YNNIFDAMLDSVYAALEKVGGSNVKIVVSESGWPSKGGDSASIDNAATYYSNLINHIKNG 27133
Query: 444 TPRRPGKALETYLFAMFNENGK 509
TP+RPG A+ETYLFAMF+EN K
Sbjct: 27134 TPKRPGAAIETYLFAMFDENQK 27199
Score = 76.7 bits (161), Expect(3) = 6e-021
Identities = 29/66 (43%), Positives = 40/66 (60%)
Frame = +3 / +3
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA +D+ +AA+EK G G+++VVSE+GWPSAGG+ G
Sbjct: 6168 YRNLFDAQLDSVYAALEKVGASGVKIVVSESGWPSAGGDSASTDNAATYYRNLINHVRNG 6347
Query: 444 TPRRPG 461
TP+RPG
Sbjct: 6348 TPKRPG 6365
Score = 74.4 bits (156), Expect(3) = 3e-021
Identities = 28/66 (42%), Positives = 39/66 (59%)
Frame = +3 / +1
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA +D+ +AA+EK G G+++VVSE+GWPSA G+ G
Sbjct: 1504 YQNLFDAQLDSVYAALEKVGASGVKIVVSESGWPSAAGDSASTDNAATYYRNLINHVKNG 1683
Query: 444 TPRRPG 461
TP+RPG
Sbjct: 1684 TPKRPG 1701
Score = 34.5 bits (69), Expect(2) = 6e-025
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +3 / +2
Query: 522 EQHFGLFQPDMSEVYHV 572
EQH+GLF PD S YHV
Sbjct: 27215 EQHYGLFNPDKSPKYHV 27265
Score = 34.5 bits (69), Expect(3) = 3e-021
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3 / +1
Query: 522 EQHFGLFQPDMSEVYHVDF 578
EQHFGLF PD S Y + F
Sbjct: 1762 EQHFGLFNPDKSPKYQISF 1818
Score = 31.8 bits (63), Expect(3) = 6e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / +3
Query: 465 ALETYLFAMFNENGK 509
A+ETYLFAMF+EN K
Sbjct: 6366 AIETYLFAMFDENQK 6410
Score = 31.8 bits (63), Expect(3) = 3e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / +1
Query: 465 ALETYLFAMFNENGK 509
A+ETYLFAMF+EN K
Sbjct: 1702 AIETYLFAMFDENQK 1746
Score = 30.9 bits (61), Expect(3) = 6e-021
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +3 / +3
Query: 522 EQHFGLFQPDMSEVYHVDF 578
EQHFGLF P+ + Y + F
Sbjct: 6426 EQHFGLFNPNRTPKYQISF 6482
>gb|AC135318.40| Medicago truncatula clone mth2-36o3, WORKING DRAFT SEQUENCE, 2 ordered
pieces
Length = 120801
Score = 67.0 bits (140), Expect(2) = 4e-024
Identities = 24/50 (48%), Positives = 36/50 (72%)
Frame = +3 / -3
Query: 231 AASTVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGE 380
A + V DG YTN+FDA+VD+ +AA+EK + + L++ ETGWP+AG +
Sbjct: 94183 AVAPVVDGELSYTNLFDAMVDSIYAALEKIDAKNVSLIIGETGWPAAGND 94034
Score = 65.7 bits (137), Expect(2) = 4e-024
Identities = 24/42 (57%), Positives = 35/42 (83%)
Frame = +3 / -3
Query: 441 GTPRRPGKALETYLFAMFNENGKAEGVEQHFGLFQPDMSEVY 566
GTPRRP +A++ ++FAMF+E+ KA GVEQ++GLF D++ VY
Sbjct: 93964 GTPRRPNQAIDAFIFAMFDEDQKAAGVEQNWGLFYHDLTPVY 93839
Score = 64.3 bits (134), Expect(2) = 9e-023
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = +3 / -1
Query: 441 GTPRRPGKALETYLFAMFNENGKAEGVEQHFGLFQPDMSEVY 566
GTPRRP +A+ +FAMF+E+ KA GVEQ++GLF DM+ VY
Sbjct: 89313 GTPRRPNQAINVLIFAMFDEDQKAAGVEQNWGLFYHDMTPVY 89188
Score = 63.8 bits (133), Expect(2) = 9e-023
Identities = 23/50 (46%), Positives = 35/50 (70%)
Frame = +3 / -3
Query: 231 AASTVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGE 380
A + V DG YT +FDA+VD+ +AA+EK + + L++ ETGWP+AG +
Sbjct: 89533 AVAPVVDGELSYTKLFDAMVDSIYAALEKIDAKDVSLIIGETGWPAAGND 89384
>gb|AC149129.3| Medicago truncatula chromosome 7 BAC clone mth2-139m18, complete
sequence
Length = 106423
Score = 67.0 bits (140), Expect(2) = 4e-024
Identities = 24/50 (48%), Positives = 36/50 (72%)
Frame = +3 / -1
Query: 231 AASTVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGE 380
A + V DG YTN+FDA+VD+ +AA+EK + + L++ ETGWP+AG +
Sbjct: 12472 AVAPVVDGELSYTNLFDAMVDSIYAALEKIDAKNVSLIIGETGWPAAGND 12323
Score = 65.7 bits (137), Expect(2) = 4e-024
Identities = 24/42 (57%), Positives = 35/42 (83%)
Frame = +3 / -1
Query: 441 GTPRRPGKALETYLFAMFNENGKAEGVEQHFGLFQPDMSEVY 566
GTPRRP +A++ ++FAMF+E+ KA GVEQ++GLF D++ VY
Sbjct: 12253 GTPRRPNQAIDAFIFAMFDEDQKAAGVEQNWGLFYHDLTPVY 12128
Score = 64.3 bits (134), Expect(2) = 9e-023
Identities = 25/42 (59%), Positives = 33/42 (78%)
Frame = +3 / -3
Query: 441 GTPRRPGKALETYLFAMFNENGKAEGVEQHFGLFQPDMSEVY 566
GTPRRP +A+ +FAMF+E+ KA GVEQ++GLF DM+ VY
Sbjct: 7604 GTPRRPNQAINVLIFAMFDEDQKAAGVEQNWGLFYHDMTPVY 7479
Score = 63.8 bits (133), Expect(2) = 9e-023
Identities = 23/50 (46%), Positives = 35/50 (70%)
Frame = +3 / -2
Query: 231 AASTVTDGGAVYTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGE 380
A + V DG YT +FDA+VD+ +AA+EK + + L++ ETGWP+AG +
Sbjct: 7824 AVAPVVDGELSYTKLFDAMVDSIYAALEKIDAKDVSLIIGETGWPAAGND 7675
>gb|BE239884.1|BE239884 EST403933 MHRP- Medicago truncatula cDNA clone pMHRP-38N17, mRNA
sequence
Length = 614
Score = 74.4 bits (156), Expect(3) = 3e-021
Identities = 28/66 (42%), Positives = 39/66 (59%)
Frame = +3 / +2
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA +D+ +AA+EK G G+++VVSE+GWPSA G+ G
Sbjct: 134 YQNLFDAQLDSVYAALEKVGASGVKIVVSESGWPSAAGDSASTDNAATYYRNLINHVKNG 313
Query: 444 TPRRPG 461
TP+RPG
Sbjct: 314 TPKRPG 331
Score = 34.5 bits (69), Expect(3) = 3e-021
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3 / +2
Query: 522 EQHFGLFQPDMSEVYHVDF 578
EQHFGLF PD S Y + F
Sbjct: 392 EQHFGLFNPDKSPKYQISF 448
Score = 31.8 bits (63), Expect(3) = 3e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / +2
Query: 465 ALETYLFAMFNENGK 509
A+ETYLFAMF+EN K
Sbjct: 332 AIETYLFAMFDENQK 376
>gb|CA921296.1|CA921296 EST639014 MTUS Medicago truncatula cDNA clone MTUS-38F3, mRNA
sequence
Length = 505
Score = 74.4 bits (156), Expect(3) = 3e-021
Identities = 28/66 (42%), Positives = 39/66 (59%)
Frame = +3 / -1
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA +D+ +AA+EK G G+++VVSE+GWPSA G+ G
Sbjct: 478 YQNLFDAQLDSVYAALEKVGASGVKIVVSESGWPSAAGDSASTDNAATYYRNLINHVKNG 299
Query: 444 TPRRPG 461
TP+RPG
Sbjct: 298 TPKRPG 281
Score = 34.5 bits (69), Expect(3) = 3e-021
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3 / -1
Query: 522 EQHFGLFQPDMSEVYHVDF 578
EQHFGLF PD S Y + F
Sbjct: 220 EQHFGLFNPDKSPKYQISF 164
Score = 31.8 bits (63), Expect(3) = 3e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / -1
Query: 465 ALETYLFAMFNENGK 509
A+ETYLFAMF+EN K
Sbjct: 280 AIETYLFAMFDENQK 236
>gb|AJ845937.1|AJ845937 AJ845937 MtSNF Medicago truncatula cDNA clone MtNF14B04N1, mRNA
sequence
Length = 555
Score = 74.4 bits (156), Expect(3) = 4e-021
Identities = 28/66 (42%), Positives = 39/66 (59%)
Frame = +3 / +3
Query: 264 YTNMFDAIVDATHAAVEKAGVQGLELVVSETGWPSAGGEGXXXXXXXXXXXXXXXXXDGG 443
Y N+FDA +D+ +AA+EK G G+++VVSE+GWPSA G+ G
Sbjct: 78 YQNLFDAQLDSVYAALEKVGASGVKIVVSESGWPSAAGDSASTDNAATYYRNLINHVKNG 257
Query: 444 TPRRPG 461
TP+RPG
Sbjct: 258 TPKRPG 275
Score = 34.1 bits (68), Expect(3) = 4e-021
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +3 / +3
Query: 522 EQHFGLFQPDMSEVYHVDF 578
EQHFGLF PD S Y + F
Sbjct: 336 EQHFGLFNPDKSPKYQIGF 392
Score = 31.8 bits (63), Expect(3) = 4e-021
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3 / +3
Query: 465 ALETYLFAMFNENGK 509
A+ETYLFAMF+EN K
Sbjct: 276 AIETYLFAMFDENQK 320
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 498,543,001
Number of Sequences: 392609
Number of extensions: 5150259
Number of successful extensions: 26708
Number of sequences better than 1.0e-020: 8
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 26973297230
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)