TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2455862.2.1
(712 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC174353.4| Medicago truncatula clone mth2-8a15, WORKING... 70 4e-026
gb|BQ137690.1|BQ137690 NF003B05NR1F1000 Nodulated root Medi... 70 5e-026
gb|CA918790.1|CA918790 EST636508 MTUS Medicago truncatula c... 70 5e-026
gb|AW981218.1|AW981218 EST392308 DSIL Medicago truncatula c... 70 5e-026
gb|CX541958.1|CX541958 s13dNF84B06GS057_467224 Germinating ... 69 1e-025
gb|BG583555.1|BG583555 EST485307 GVN Medicago truncatula cD... 68 5e-024
gb|CA921291.1|CA921291 EST639009 MTUS Medicago truncatula c... 67 1e-023
gb|AW299181.1|AW299181 EST305991 KV2 Medicago truncatula cD... 67 1e-023
gb|CA922506.1|CA922506 EST640224 MTUS Medicago truncatula c... 67 1e-023
gb|AC148761.1| Medicago truncatula chromosome 2 clone mth2-... 66 4e-023
gb|BG647282.1|BG647282 EST508901 HOGA Medicago truncatula c... 70 9e-023
gb|BQ165643.1|BQ165643 EST611512 KVKC Medicago truncatula c... 65 4e-022
emb|CR969619.1| mth4-23B8FM1 BAC end, cultivar Jemalong A17... 65 4e-022
gb|CX522122.1|CX522122 s13dNF77D10VI080_470048 Virus-Infect... 65 4e-022
gb|AW574197.1|AW574197 EST316788 GVN Medicago truncatula cD... 63 8e-022
emb|CR299174.1| mte1-17C1FM1 BAC end, cultivar Jemalong A17... 65 1e-021
>gb|AC174353.4| Medicago truncatula clone mth2-8a15, WORKING DRAFT SEQUENCE, 17
unordered pieces
Length = 101872
Score = 70.3 bits (147), Expect(2) = 4e-026
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +1 / -1
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACV 162
+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+
Sbjct: 39289 FPKNQPMRIYSSLWNADNWATRGGLVKTDWTKAPFTASYRNFNAQACI 39146
Score = 68.9 bits (144), Expect(2) = 4e-026
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +1 / -1
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
W+S++LDA G + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 39079 WLSQELDATGQGRLKWVQKNYMIYNYCKDTKRFPQGLPPECNHS* 38945
Score = 67.0 bits (140), Expect(2) = 9e-024
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +1 / -2
Query: 196 AGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
A + W+S+QLD+ + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 42219 ASSASWLSQQLDSTGQQRLRWVQKNYMIYNYCSDKKRFPQGLPVECTHS* 42070
Score = 65.2 bits (136), Expect(2) = 4e-022
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +1 / +1
Query: 13 LAYPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+A+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A+
Sbjct: 30580 VAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAE 30720
Score = 64.3 bits (134), Expect(2) = 9e-024
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1 / -2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+
Sbjct: 42411 FPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNAN 42277
Score = 60.6 bits (126), Expect(2) = 4e-022
Identities = 22/54 (40%), Positives = 34/54 (62%)
Frame = +1 / +1
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FIGLLV 363
++ W ++QLD+ ++ ++NYM YNYC D RFPQG P EC+ +* I +
Sbjct: 30730 SNAWFTQQLDSTSQQRLSEVQKNYMIYNYCTDIKRFPQGLPTECTAS*LISFYI 30891
>gb|BQ137690.1|BQ137690 NF003B05NR1F1000 Nodulated root Medicago truncatula cDNA clone
NF003B05NR 5', mRNA sequence
Length = 1133
Score = 70.3 bits (147), Expect(2) = 5e-026
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +1 / +2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACV 162
+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+
Sbjct: 131 FPKNQPMRIYSSLWNADNWATRGGLVKTDWTKAPFTASYRNFNAQACI 274
Score = 68.9 bits (144), Expect(2) = 5e-026
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +1 / +2
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
W+S++LDA G + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 341 WLSQELDATGQGRLKWVQKNYMIYNYCKDTKRFPQGLPPECNHS* 475
>gb|CA918790.1|CA918790 EST636508 MTUS Medicago truncatula cDNA clone MTUS-3C4, mRNA
sequence
Length = 757
Score = 70.3 bits (147), Expect(2) = 5e-026
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +1 / -3
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACV 162
+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+
Sbjct: 500 FPKNQPMRIYSSLWNADNWATRGGLVKTDWTKAPFTASYRNFNAQACI 357
Score = 68.9 bits (144), Expect(2) = 5e-026
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +1 / -3
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
W+S++LDA G + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 290 WLSQELDATGQGRLKWVQKNYMIYNYCKDTKRFPQGLPPECNHS* 156
>gb|AW981218.1|AW981218 EST392308 DSIL Medicago truncatula cDNA clone pDSIL-12C3, mRNA
sequence
Length = 570
Score = 70.3 bits (147), Expect(2) = 5e-026
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +1 / -2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACV 162
+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+
Sbjct: 524 FPKNQPMRIYSSLWNADNWATRGGLVKTDWTKAPFTASYRNFNAQACI 381
Score = 68.9 bits (144), Expect(2) = 5e-026
Identities = 24/45 (53%), Positives = 32/45 (71%)
Frame = +1 / -2
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
W+S++LDA G + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 314 WLSQELDATGQGRLKWVQKNYMIYNYCKDTKRFPQGLPPECNHS* 180
>gb|CX541958.1|CX541958 s13dNF84B06GS057_467224 Germinating Seed Medicago truncatula cDNA,
mRNA sequence
Length = 591
Score = 69.3 bits (145), Expect(2) = 1e-025
Identities = 22/48 (45%), Positives = 34/48 (70%)
Frame = +1 / +3
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACV 162
+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+
Sbjct: 249 FPKNQPMRIYSSLWNADNWATRGGLVKTDWTKAPFTASYRKFNAQACI 392
Score = 68.4 bits (143), Expect(2) = 1e-025
Identities = 23/44 (52%), Positives = 31/44 (70%)
Frame = +1 / +3
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN 342
W+S++LDA G + W ++NYM YNYC D RFPQG P EC+ +
Sbjct: 459 WLSQELDATGQGRLKWVQKNYMIYNYCKDTKRFPQGLPPECNHS 590
>gb|BG583555.1|BG583555 EST485307 GVN Medicago truncatula cDNA clone pGVN-73N11 5' end,
mRNA sequence
Length = 720
Score = 68.0 bits (142), Expect(2) = 5e-024
Identities = 25/52 (48%), Positives = 34/52 (65%)
Frame = +1 / +2
Query: 196 AGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FI 351
A + W+S+QLD+ + W ++NYM YNYC D RFPQG P EC+ +* I
Sbjct: 362 ASSASWLSQQLDSTGQQRLRWVQKNYMIYNYCSDKKRFPQGLPVECTHS*SI 517
Score = 64.3 bits (134), Expect(2) = 5e-024
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1 / +2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+
Sbjct: 170 FPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNAN 304
>gb|CA921291.1|CA921291 EST639009 MTUS Medicago truncatula cDNA clone MTUS-38E9, mRNA
sequence
Length = 705
Score = 67.0 bits (140), Expect(2) = 1e-023
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +1 / -2
Query: 196 AGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
A + W+S+QLD+ + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 329 ASSASWLSQQLDSTGQQRLRWVQKNYMIYNYCSDKKRFPQGLPVECTHS* 180
Score = 64.3 bits (134), Expect(2) = 1e-023
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1 / -2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+
Sbjct: 521 FPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNAN 387
>gb|AW299181.1|AW299181 EST305991 KV2 Medicago truncatula cDNA clone KV2-9J15, mRNA
sequence
Length = 695
Score = 67.0 bits (140), Expect(2) = 1e-023
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +1 / +1
Query: 196 AGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
A + W+S+QLD+ + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 382 ASSASWLSQQLDSTGQQRLRWVQKNYMIYNYCSDKKRFPQGLPVECTHS* 531
Score = 64.3 bits (134), Expect(2) = 1e-023
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1 / +1
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+
Sbjct: 190 FPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNAN 324
>gb|CA922506.1|CA922506 EST640224 MTUS Medicago truncatula cDNA clone MTUS-54E11, mRNA
sequence
Length = 669
Score = 67.0 bits (140), Expect(2) = 1e-023
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +1 / -2
Query: 196 AGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN* 345
A + W+S+QLD+ + W ++NYM YNYC D RFPQG P EC+ +*
Sbjct: 362 ASSASWLSQQLDSTGQQRLRWVQKNYMIYNYCSDKKRFPQGLPVECTHS* 213
Score = 64.3 bits (134), Expect(2) = 1e-023
Identities = 21/45 (46%), Positives = 32/45 (71%)
Frame = +1 / -2
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+
Sbjct: 554 FPKNQPMRIYSSLWNADDWATRGGLVKTDWSKAPFTASYRNFNAN 420
>gb|AC148761.1| Medicago truncatula chromosome 2 clone mth2-19h23, *** SEQUENCING IN
PROGRESS ***, 14 unordered pieces
Length = 125354
Score = 65.7 bits (137), Expect(2) = 4e-023
Identities = 23/57 (40%), Positives = 35/57 (61%)
Frame = +1 / +1
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADACVPDGDGRPLS 189
+P + M ++++LW+ WAT+ G VK DW+ APF SYR ++A AC+ G S
Sbjct: 66889 FPKNQAMRIYSSLWNADNWATRGGIVKTDWTNAPFIASYRNFNAKACIWTSSGSSCS 67059
Score = 63.4 bits (132), Expect(2) = 4e-023
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +1 / +1
Query: 211 WMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAEC 333
W+ LD+ + W ++NYM YNYC D RFPQGFPAEC
Sbjct: 67099 WLRESLDSKGIRKIRWVQKNYMIYNYCTDYKRFPQGFPAEC 67221
>gb|BG647282.1|BG647282 EST508901 HOGA Medicago truncatula cDNA clone pHOGA-16O5 5' end,
mRNA sequence
Length = 759
Score = 69.8 bits (146), Expect(2) = 9e-023
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = +1 / +1
Query: 19 YPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADAC 159
+P +PM ++++LW+ WAT+ G VK DWS APF SYR ++A+AC
Sbjct: 430 FPKSQPMRIYSSLWNADDWATRGGLVKTDWSNAPFTASYRNFNANAC 570
Score = 58.3 bits (121), Expect(2) = 9e-023
Identities = 22/57 (38%), Positives = 30/57 (52%)
Frame = +1 / +1
Query: 160 VPDGDGRPLSCPAGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAE 330
V G S + + W S +LD+ + W ++NYM YNYC D RFPQG P +
Sbjct: 574 VSSGTSSCSSTQSNKNSWFSEELDSTSHERLKWVQKNYMIYNYCTDTKRFPQGLPQD 744
>gb|BQ165643.1|BQ165643 EST611512 KVKC Medicago truncatula cDNA clone pKVKC-10F10, mRNA
sequence
Length = 800
Score = 65.2 bits (136), Expect(2) = 4e-022
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +1 / -2
Query: 13 LAYPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+A+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A+
Sbjct: 415 VAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAE 275
Score = 60.6 bits (126), Expect(2) = 4e-022
Identities = 22/54 (40%), Positives = 34/54 (62%)
Frame = +1 / -2
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FIGLLV 363
++ W ++QLD+ ++ ++NYM YNYC D RFPQG P EC+ +* I +
Sbjct: 265 SNAWFTQQLDSTSQQRLSEVQKNYMIYNYCTDIKRFPQGLPTECTAS*LISFYI 104
>emb|CR969619.1| mth4-23B8FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 772
Score = 65.2 bits (136), Expect(2) = 4e-022
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +1 / -3
Query: 13 LAYPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+A+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A+
Sbjct: 449 VAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAE 309
Score = 60.6 bits (126), Expect(2) = 4e-022
Identities = 22/54 (40%), Positives = 34/54 (62%)
Frame = +1 / -3
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FIGLLV 363
++ W ++QLD+ ++ ++NYM YNYC D RFPQG P EC+ +* I +
Sbjct: 299 SNAWFTQQLDSTSQQRLSEVQKNYMIYNYCTDIKRFPQGLPTECTAS*LISFYI 138
>gb|CX522122.1|CX522122 s13dNF77D10VI080_470048 Virus-Infected Leaves Medicago truncatula
cDNA, mRNA sequence
Length = 607
Score = 65.2 bits (136), Expect(2) = 4e-022
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +1 / +1
Query: 13 LAYPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+A+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A+
Sbjct: 160 VAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAE 300
Score = 60.6 bits (126), Expect(2) = 4e-022
Identities = 22/54 (40%), Positives = 34/54 (62%)
Frame = +1 / +1
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FIGLLV 363
++ W ++QLD+ ++ ++NYM YNYC D RFPQG P EC+ +* I +
Sbjct: 310 SNAWFTQQLDSTSQQRLSEVQKNYMIYNYCTDIKRFPQGLPTECTAS*LISFYI 471
>gb|AW574197.1|AW574197 EST316788 GVN Medicago truncatula cDNA clone pGVN-51L17, mRNA
sequence
Length = 496
Score = 62.9 bits (131), Expect(2) = 8e-022
Identities = 22/49 (44%), Positives = 29/49 (59%)
Frame = +1 / +3
Query: 187 SCPAGTDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAEC 333
S + + W S +LD+ + W ++NYM YNYC D RFPQG P EC
Sbjct: 165 STQSNKNSWFSEELDSTSHERLKWVQKNYMIYNYCTDTKRFPQGLPQEC 311
Score = 62.0 bits (129), Expect(2) = 8e-022
Identities = 21/41 (51%), Positives = 30/41 (73%)
Frame = +1 / +3
Query: 37 MSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSADAC 159
M ++++LW+ WAT+ G VK DWS APF SYR ++A+AC
Sbjct: 12 MLIYSSLWNADDWATRGGLVKTDWSNAPFTASYRNFNANAC 134
>emb|CR299174.1| mte1-17C1FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 722
Score = 65.2 bits (136), Expect(2) = 1e-021
Identities = 21/47 (44%), Positives = 34/47 (72%)
Frame = +1 / +1
Query: 13 LAYPGGKPMSVHATLWDGSYWATQQGKVKVDWSAAPFAVSYRGYSAD 153
+A+P +PM ++++LW+ WAT+ G VK DW+ APF SYR ++A+
Sbjct: 391 VAFPKNQPMRIYSSLWNADDWATRGGLVKTDWTNAPFTASYRNFNAE 531
Score = 58.8 bits (122), Expect(2) = 1e-021
Identities = 21/54 (38%), Positives = 34/54 (62%)
Frame = +1 / +1
Query: 202 TDRWMSRQLDAAEWGTVAWARQNYMHYNYCDDGWRFPQGFPAECSRN*FIGLLV 363
++ W ++QLD+ ++ ++NYM YNYC D RFPQG P +C+ +* I +
Sbjct: 541 SNAWFTQQLDSTSQQRLSEVQKNYMIYNYCTDIKRFPQGLPTKCTAS*LISFYI 702
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 521,412,789
Number of Sequences: 392609
Number of extensions: 6331071
Number of successful extensions: 38082
Number of sequences better than 1.0e-020: 19
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 22939906990
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)