TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448655.2.1
(682 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC152498.11| Medicago truncatula clone mth2-38m20, compl... 143 6e-062
gb|AW560211.1|AW560211 EST315251 DSIR Medicago truncatula c... 162 6e-039
gb|AJ497568.1|AJ497568 AJ497568 MTFLOW Medicago truncatula ... 162 6e-039
gb|CA920379.1|CA920379 EST638097 MTUS Medicago truncatula c... 161 1e-038
gb|AC146723.24| Medicago truncatula clone mth2-34n4, WORKIN... 102 1e-025
gb|AC126785.11| Medicago truncatula clone mth2-9i24, comple... 100 1e-025
gb|CG940173.1|CG940173 MBEJW07TF mth2 Medicago truncatula g... 110 2e-023
gb|BG449670.1|BG449670 NF055F06IN1F1057 Insect herbivory Me... 81 1e-021
>gb|AC152498.11| Medicago truncatula clone mth2-38m20, complete sequence
Length = 127132
Score = 143 bits (307), Expect(2) = 6e-062
Identities = 54/67 (80%), Positives = 61/67 (91%)
Frame = +2 / +2
Query: 2 VHHEFTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGF 181
VHHE+ APIGFAAPYAHWAE+LILGIPSF+GPA+VPGHMITFWLWI LRQVEAI+THSG+
Sbjct: 65564 VHHEYQAPIGFAAPYAHWAEILILGIPSFLGPAMVPGHMITFWLWIALRQVEAIDTHSGY 65743
Query: 182 DFPFTPT 202
F P+
Sbjct: 65744 YFCHFPS 65764
Score = 125 bits (267), Expect(2) = 6e-062
Identities = 44/49 (89%), Positives = 48/49 (97%)
Frame = +2 / +3
Query: 179 FDFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDK 325
+DFP+ PTKYIPFYGGAE+HDYHHYVGGQSQSNFASVFTYCDY+YGTDK
Sbjct: 67449 YDFPWNPTKYIPFYGGAEHHDYHHYVGGQSQSNFASVFTYCDYIYGTDK 67595
Score = 90.0 bits (190), Expect(2) = 1e-030
Identities = 38/53 (71%), Positives = 44/53 (83%)
Frame = -2 / -2
Query: 177 PLCVSIAST*RSIIQSQNVIMWPGTMAGPTKEGMPSISTSAQCAYGAANPIGA 19
PLCVS+AST R+ I +QNVIM PGT+AGP K+G+P I SAQCA GAANPIGA
Sbjct: 65739 PLCVSMASTWRNAIHNQNVIM*PGTIAGPRKDGIPKIKISAQCA*GAANPIGA 65581
Score = 73.9 bits (155), Expect(2) = 1e-030
Identities = 37/48 (77%), Positives = 39/48 (81%)
Frame = -2 / -1
Query: 324 LSVPYK*SQ*VKTEAKLLWLWPPT****SWYSAPP*NGIYFVGVNGKS 181
LSVP *SQ VKT+AKLLWL PPT** *S SAPP*NG+YFVG GKS
Sbjct: 67594 LSVP*M*SQYVKTDAKLLWLCPPT**W*S*CSAPP*NGMYFVGFQGKS 67451
>gb|AW560211.1|AW560211 EST315251 DSIR Medicago truncatula cDNA clone pDSIR-26M4, mRNA
sequence
Length = 656
Score = 162 bits (348), Expect = 6e-039
Identities = 55/111 (49%), Positives = 78/111 (70%)
Frame = +2 / -2
Query: 2 VHHEFTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGF 181
VHHE+ P G + YAH AE+L LG + VGPAI H+IT WLW++LR +E +E H G+
Sbjct: 598 VHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGY 419
Query: 182 DFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYR 334
FP++P+ ++P YGG+++HDYHH + N++S FTY D ++GTD GYR
Sbjct: 418 HFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDIGYR 266
>gb|AJ497568.1|AJ497568 AJ497568 MTFLOW Medicago truncatula cDNA clone mt--abc955111a03,
mRNA sequence
Length = 495
Score = 162 bits (348), Expect = 6e-039
Identities = 55/111 (49%), Positives = 78/111 (70%)
Frame = +2 / +1
Query: 2 VHHEFTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGF 181
VHHE+ P G + YAH AE+L LG + VGPAI H+IT WLW++LR +E +E H G+
Sbjct: 52 VHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGY 231
Query: 182 DFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYR 334
FP++P+ ++P YGG+++HDYHH + N++S FTY D ++GTD GYR
Sbjct: 232 HFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDIGYR 384
>gb|CA920379.1|CA920379 EST638097 MTUS Medicago truncatula cDNA clone MTUS-27E3, mRNA
sequence
Length = 680
Score = 161 bits (346), Expect = 1e-038
Identities = 54/111 (48%), Positives = 78/111 (70%)
Frame = +2 / -2
Query: 2 VHHEFTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGF 181
VHHE+ P G + YAH AE+L LG + VGPAI H+IT WLW+++R +E +E H G+
Sbjct: 517 VHHEYATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVVRVLETVEAHCGY 338
Query: 182 DFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYR 334
FP++P+ ++P YGG+++HDYHH + N++S FTY D ++GTD GYR
Sbjct: 337 HFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYR 185
>gb|AC146723.24| Medicago truncatula clone mth2-34n4, WORKING DRAFT SEQUENCE, 3 ordered
pieces
Length = 121085
Score = 102 bits (217), Expect(2) = 1e-025
Identities = 34/71 (47%), Positives = 49/71 (69%)
Frame = +2 / +2
Query: 14 FTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGFDFPF 193
+ P G + YAH AE+L LG + VGPAI H+IT WLW++LR +E +E H G+ FP+
Sbjct: 85664 YATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVLRVLETVEAHCGYHFPW 85843
Query: 194 TPTKYIPFYGG 226
+P+ ++P YGG
Sbjct: 85844 SPSNFLPLYGG 85876
Score = 35.4 bits (71), Expect(2) = 1e-025
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2 / +2
Query: 227 AEYHDYHHYVGGQSQSNFASVFTYCD 304
+++HDYHH + N++S FTY D
Sbjct: 85976 SDFHDYHHRLLYTKSGNYSSTFTYMD 86053
>gb|AC126785.11| Medicago truncatula clone mth2-9i24, complete sequence
Length = 132702
Score = 100 bits (214), Expect(2) = 1e-025
Identities = 33/71 (46%), Positives = 49/71 (69%)
Frame = +2 / +2
Query: 14 FTAPIGFAAPYAHWAEVLILGIPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGFDFPF 193
+ P G + YAH AE+L LG + VGPAI H+IT WLW+++R +E +E H G+ FP+
Sbjct: 103919 YATPFGLTSEYAHPAEILFLGFATIVGPAITGPHLITLWLWMVVRVLETVEAHCGYHFPW 104098
Query: 194 TPTKYIPFYGG 226
+P+ ++P YGG
Sbjct: 104099 SPSNFLPLYGG 104131
Score = 36.3 bits (73), Expect(2) = 1e-025
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2 / +2
Query: 212 PFYGGAEYHDYHHYVGGQSQSNFASVFTYCD 304
P + +++HDYHH + N++S FTY D
Sbjct: 104216 PQFFRSDFHDYHHRLLYTKSGNYSSTFTYMD 104308
>gb|CG940173.1|CG940173 MBEJW07TF mth2 Medicago truncatula genomic clone 71B13, DNA
sequence
Length = 892
Score = 110 bits (235), Expect = 2e-023
Identities = 38/49 (77%), Positives = 44/49 (89%)
Frame = +2 / -3
Query: 179 FDFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDK 325
++ P++PTKYIPFYGG YHDYHHYVGG+SQ NFASVFTYCDY+YGT K
Sbjct: 788 YELPWSPTKYIPFYGGPAYHDYHHYVGGRSQGNFASVFTYCDYIYGTIK 642
>gb|BG449670.1|BG449670 NF055F06IN1F1057 Insect herbivory Medicago truncatula cDNA clone
NF055F06IN 5', mRNA sequence
Length = 664
Score = 80.8 bits (170), Expect(2) = 1e-021
Identities = 25/54 (46%), Positives = 40/54 (74%)
Frame = +2 / -1
Query: 173 SGFDFPFTPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYLYGTDKGYR 334
+G+ FP++P+ ++P YGG+++HDYHH + N++S FTY D ++GTD GYR
Sbjct: 526 AGYHFPWSPSNFLPLYGGSDFHDYHHRLLYTKSGNYSSTFTYMDRIFGTDVGYR 365
Score = 43.7 bits (89), Expect(2) = 1e-021
Identities = 15/37 (40%), Positives = 23/37 (62%)
Frame = +2 / -3
Query: 77 IPSFVGPAIVPGHMITFWLWIILRQVEAIETHSGFDF 187
+ + VGPAI H+ T WL +++R +E +E H G F
Sbjct: 623 VATIVGPAITGPHLKTLWLXMVVRVLETVEAHCGLPF 513
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 714,883,633
Number of Sequences: 392609
Number of extensions: 10275656
Number of successful extensions: 49153
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 21679472540
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)