TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2437629.2.2
(1041 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ154720.1|BQ154720 NF099G01IR1F1008 Irradiated Medicago... 130 3e-036
gb|CX523674.1|CX523674 s13dNF02F03AT029_322732 Aphid-Infect... 130 3e-036
gb|CA922013.1|CA922013 EST639731 MTUS Medicago truncatula c... 130 3e-036
gb|BI311684.1|BI311684 EST5313434 GESD Medicago truncatula ... 133 1e-033
gb|AC137820.11| Medicago truncatula clone mth2-15f9, comple... 82 4e-028
gb|CA990471.1|CA990471 EST643979 GESD Medicago truncatula c... 122 7e-027
gb|AC155101.19| Medicago truncatula clone mth2-139j13, comp... 90 1e-026
gb|BF519079.1|BF519079 EST456539 DSIL Medicago truncatula c... 121 3e-026
gb|CA990480.1|CA990480 EST643988 GESD Medicago truncatula c... 120 3e-026
gb|BE324374.2|BE324374 NF015G01PL1F1005 Phosphate starved l... 114 2e-024
>gb|BQ154720.1|BQ154720 NF099G01IR1F1008 Irradiated Medicago truncatula cDNA clone
NF099G01IR 5', mRNA sequence
Length = 666
Score = 130 bits (279), Expect(2) = 3e-036
Identities = 52/69 (75%), Positives = 59/69 (85%)
Frame = +1 / +1
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS RPNLS
Sbjct: 286 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLS 465
Query: 184 IREGIGMVV 210
IREGI ++V
Sbjct: 466 IREGIEIIV 492
Score = 46.0 bits (94), Expect(2) = 3e-036
Identities = 19/32 (59%), Positives = 25/32 (78%)
Frame = +3 / +1
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMPQVI+ +E + ++ I LNR+
Sbjct: 481 EIIVSGGMTMPQVISPIERVAHPNERISLNRI 576
>gb|CX523674.1|CX523674 s13dNF02F03AT029_322732 Aphid-Infected Shoots Medicago truncatula
cDNA, mRNA sequence
Length = 692
Score = 130 bits (279), Expect(2) = 3e-036
Identities = 52/69 (75%), Positives = 59/69 (85%)
Frame = +1 / -2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS RPNLS
Sbjct: 505 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLS 326
Query: 184 IREGIGMVV 210
IREGI ++V
Sbjct: 325 IREGIEIIV 299
Score = 46.0 bits (94), Expect(2) = 3e-036
Identities = 19/32 (59%), Positives = 25/32 (78%)
Frame = +3 / -2
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMPQVI+ +E + ++ I LNR+
Sbjct: 310 EIIVSGGMTMPQVISPIERVAHPNERISLNRI 215
>gb|CA922013.1|CA922013 EST639731 MTUS Medicago truncatula cDNA clone MTUS-47E10, mRNA
sequence
Length = 779
Score = 130 bits (279), Expect(2) = 3e-036
Identities = 52/69 (75%), Positives = 59/69 (85%)
Frame = +1 / -2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS RPNLS
Sbjct: 481 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLS 302
Query: 184 IREGIGMVV 210
IREGI ++V
Sbjct: 301 IREGIEIIV 275
Score = 46.0 bits (94), Expect(2) = 3e-036
Identities = 19/32 (59%), Positives = 25/32 (78%)
Frame = +3 / -2
Query: 396 EIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
EIIVSGGMTMPQVI+ +E + ++ I LNR+
Sbjct: 286 EIIVSGGMTMPQVISPIERVAHPNERISLNRI 191
>gb|BI311684.1|BI311684 EST5313434 GESD Medicago truncatula cDNA clone pGESD13J1 5' end,
mRNA sequence
Length = 687
Score = 133 bits (286), Expect(2) = 1e-033
Identities = 55/69 (79%), Positives = 60/69 (86%)
Frame = +1 / +1
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYA GFITS+VVLQT GDEELC VYVPTNHLYIGDIFLVN+ RPNLS
Sbjct: 205 AIIRHPRVGEYALGFITSSVVLQTYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRPNLS 384
Query: 184 IREGIGMVV 210
+REGI +VV
Sbjct: 385 VREGIEIVV 411
Score = 34.1 bits (68), Expect(2) = 1e-033
Identities = 11/18 (61%), Positives = 18/18 (100%)
Frame = +3 / +1
Query: 396 EIIVSGGMTMPQVIASLE 449
EI+VSGGM+MPQ++++L+
Sbjct: 400 EIVVSGGMSMPQILSTLD 453
>gb|AC137820.11| Medicago truncatula clone mth2-15f9, complete sequence
Length = 134674
Score = 82.2 bits (173), Expect(3) = 4e-028
Identities = 32/44 (72%), Positives = 36/44 (81%)
Frame = +1 / -3
Query: 70 QTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSIREGIG 201
Q + DEELCSV++PTNHLYIGDI L+NS RPNLSIREGIG
Sbjct: 117650 QKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLSIREGIG 117519
Score = 53.8 bits (111), Expect(3) = 4e-028
Identities = 21/23 (91%), Positives = 22/23 (95%)
Frame = +1 / -2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQ 72
AIIRHPR+GEYAFGFITSTV LQ
Sbjct: 118878 AIIRHPRVGEYAFGFITSTVTLQ 118810
Score = 47.8 bits (98), Expect(3) = 4e-028
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +3 / -2
Query: 393 AEIIVSGGMTMPQVIASLEPMPRKSQNIRLNRM 491
AEIIVSGGMTMPQVI+ +E + ++ I LNR+
Sbjct: 117291 AEIIVSGGMTMPQVISPIERVAHPNERISLNRI 117193
>gb|CA990471.1|CA990471 EST643979 GESD Medicago truncatula cDNA clone GESD-28D22, mRNA
sequence
Length = 821
Score = 122 bits (261), Expect(2) = 7e-027
Identities = 49/64 (76%), Positives = 54/64 (84%)
Frame = +1 / +2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS R NLS
Sbjct: 542 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSKDVIRTNLS 721
Query: 184 IREG 195
IREG
Sbjct: 722 IREG 733
Score = 23.1 bits (44), Expect(2) = 7e-027
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +3 / +3
Query: 417 MTMPQVIASLEPMPRKSQNI 476
MTMPQVI+ +E ++ I
Sbjct: 756 MTMPQVISPIERSAHPNEEI 815
>gb|AC155101.19| Medicago truncatula clone mth2-139j13, complete sequence
Length = 124152
Score = 90.4 bits (191), Expect(3) = 1e-026
Identities = 40/80 (50%), Positives = 53/80 (66%)
Frame = +1 / -1
Query: 46 FITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSIREGIGMVVWLI*C 225
++ V QT GDEELC VYVPTNHLYIGDIFLVN+ RPNLS+REGIG + +
Sbjct: 113136 YLNYIAVSQTYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRPNLSVREGIGELY*FLKL 112957
Query: 226 LASLYYYAMWQLSLKFLLLV 285
+L+ + +L+ L+L+
Sbjct: 112956 F*ALHCRSKGRLNYVALILI 112897
Score = 83.5 bits (176), Expect(2) = 2e-022
Identities = 33/46 (71%), Positives = 37/46 (80%)
Frame = +1 / -3
Query: 64 VLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLSIREGIG 201
+ Q+ GDEELC VYVPTNHLYIGDIFLVN+ RP LS+REGIG
Sbjct: 104140 LFQSYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRPTLSVREGIG 104003
Score = 54.2 bits (112), Expect(2) = 2e-022
Identities = 21/26 (80%), Positives = 24/26 (92%)
Frame = +1 / -2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDK 81
AIIRHPR+GEYAFGFITS+VVLQ +
Sbjct: 107057 AIIRHPRVGEYAFGFITSSVVLQVSE 106980
Score = 51.0 bits (105), Expect(3) = 1e-026
Identities = 20/23 (86%), Positives = 22/23 (95%)
Frame = +1 / -3
Query: 4 AIIRHPRIGEYAFGFITSTVVLQ 72
AIIRHPR+GEYA GFITS+VVLQ
Sbjct: 113437 AIIRHPRVGEYALGFITSSVVLQ 113369
Score = 37.3 bits (75), Expect(3) = 1e-026
Identities = 13/19 (68%), Positives = 19/19 (100%)
Frame = +3 / -1
Query: 393 AEIIVSGGMTMPQVIASLE 449
AEI+VSGGM+MPQ++++LE
Sbjct: 103539 AEIVVSGGMSMPQILSTLE 103483
>gb|BF519079.1|BF519079 EST456539 DSIL Medicago truncatula cDNA clone pDSIL-19L9, mRNA
sequence
Length = 651
Score = 121 bits (258), Expect = 3e-026
Identities = 48/62 (77%), Positives = 53/62 (85%)
Frame = +1 / +1
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS RPNLS
Sbjct: 466 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSKDVIRPNLS 645
Query: 184 IR 189
IR
Sbjct: 646 IR 651
>gb|CA990480.1|CA990480 EST643988 GESD Medicago truncatula cDNA clone GESD-28F22, mRNA
sequence
Length = 712
Score = 120 bits (257), Expect = 3e-026
Identities = 49/65 (75%), Positives = 54/65 (83%)
Frame = +1 / +2
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRPNLS 183
AIIRHPR+GEYAFGFITSTV LQ + DEELCSV++PTNHLYIGDI L+NS R NLS
Sbjct: 515 AIIRHPRVGEYAFGFITSTVTLQKENEDEELCSVFIPTNHLYIGDIILINSRDVIRTNLS 694
Query: 184 IREGI 198
IRE I
Sbjct: 695 IRERI 709
>gb|BE324374.2|BE324374 NF015G01PL1F1005 Phosphate starved leaf Medicago truncatula cDNA
clone NF015G01PL 5', mRNA sequence
Length = 346
Score = 114 bits (244), Expect = 2e-024
Identities = 46/57 (80%), Positives = 50/57 (87%)
Frame = +1 / +3
Query: 4 AIIRHPRIGEYAFGFITSTVVLQTDKGDEELCSVYVPTNHLYIGDIFLVNSXXXXRP 174
AIIRHPR+GEYAFGFITS+VVLQ+ GDEELC VYVPTNHLYIGDIFLVN+ RP
Sbjct: 171 AIIRHPRVGEYAFGFITSSVVLQSYSGDEELCCVYVPTNHLYIGDIFLVNTKDVIRP 341
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,198,397,463
Number of Sequences: 392609
Number of extensions: 19460950
Number of successful extensions: 111136
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 36564393276
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)