TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404928.2.2
(1151 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CT573076.1| Medicago truncatula chromosome 5 clone mth2... 198 e-128
gb|CX529733.1|CX529733 s13dNF52B09MJ074_245512 Methyl Jasmo... 336 3e-091
gb|BQ154941.1|BQ154941 NF074E06IR1F1051 Irradiated Medicago... 214 1e-082
gb|CX533112.1|CX533112 s13dNF0CA03MJ017_319389 Methyl Jasmo... 184 3e-045
emb|CR479179.1| mth2-189M5RM1 BAC end, cultivar Jemalong A1... 160 4e-038
emb|CR479743.1| mth2-190I5FM1 BAC end, cultivar Jemalong A1... 140 5e-032
gb|AW694998.1|AW694998 NF082D04ST1F1041 Developing stem Med... 112 1e-023
gb|AW191221.1|AW191221 T113396e KV2 Medicago truncatula cDN... 108 2e-022
gb|AW191214.1|AW191214 T113386e KV2 Medicago truncatula cDN... 106 5e-022
>emb|CT573076.1| Medicago truncatula chromosome 5 clone mth2-181d5, COMPLETE SEQUENCE
Length = 89591
Score = 198 bits (427), Expect(6) = e-128
Identities = 81/96 (84%), Positives = 90/96 (93%)
Frame = -3 / +2
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 16646 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 16825
Query: 969 NPDTLKKLNEAERAKKELEQQEYYDPKLADEEREKG 862
NPDTLKKLNEAE+AKKELEQQEY+DP LADEEREKG
Sbjct: 16826 NPDTLKKLNEAEKAKKELEQQEYFDPNLADEEREKG 16933
Score = 171 bits (367), Expect(6) = e-128
Identities = 67/78 (85%), Positives = 73/78 (93%)
Frame = -3 / +2
Query: 780 RVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETYQAG 601
+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFTKGYTRKGA+QFFMKEY+KA+ETYQ G
Sbjct: 17234 QAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFTKGYTRKGAVQFFMKEYEKALETYQEG 17413
Query: 600 LKHDPKNQELLDGVRRCI 547
LKHD NQELL+GVRR I
Sbjct: 17414 LKHDANNQELLEGVRRYI 17467
Score = 112 bits (239), Expect(5) = 1e-065
Identities = 59/97 (60%), Positives = 61/97 (62%)
Frame = +2 / -3
Query: 860 LPFSLSSSANFGS*YXXXXXXXXXXXXXXXXXRVSGFRCSVRAFW*VSMAISKSLEVLAS 1039
+PFSLSSSA FGS Y RVSG RCSV AFW*VSM S SLE A
Sbjct: 16935 VPFSLSSSAKFGSKYSCCSSSFLAFSASLSFFRVSGLRCSVSAFW*VSMTGS*SLEHFAV 16756
Query: 1040 LTRAVPFLVSALEIILKSARSSLPLSTALSQSLMHSS 1150
L AVPFLV AL IIL SA +S PLSTALSQS M SS
Sbjct: 16755 LPMAVPFLVKALAIIL*SALNSRPLSTALSQSFMQSS 16645
Score = 112 bits (239), Expect(2) = 1e-030
Identities = 46/117 (39%), Positives = 71/117 (60%)
Frame = -3 / +2
Query: 909 QEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMP 730
Q +D +ADE + KGN F + AI+H++EA+ +P + +YSNR+A Y L
Sbjct: 13943 QNPFDLSMADEAKAKGNAAFSSGDFSTAIRHFSEAIDLSPTNHVLYSNRSAAYASLQNYT 14122
Query: 729 EGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETYQAGLKHDPKNQELLDGV 559
+ L DA+K +EL P ++KGY+R GA + +YD A+ Y+ GL+ DP N+ L G+
Sbjct: 14123 DALTDAKKTVELKPDWSKGYSRLGAAHLGLSQYDDAVSAYKKGLEIDPNNEPLKSGL 14293
Score = 110 bits (235), Expect(5) = 1e-065
Identities = 48/66 (72%), Positives = 54/66 (81%)
Frame = +2 / -3
Query: 548 MHLLTPSSSSWFFGSCFRPA**VSMALSYSFMKN*IAPFLVYPLVKVGSSSRHFSASLRP 727
M+L TPSS+SW F SCF P+* VS A SYSFMKN* APF V PLVKVGSSS HFSAS +P
Sbjct: 17466 MYLRTPSSNSWLFASCFNPS*YVSKAFSYSFMKN*TAPFRV*PLVKVGSSSIHFSASFKP 17287
Query: 728 SGMAPN 745
SG+AP+
Sbjct: 17286 SGIAPS 17269
Score = 79.0 bits (166), Expect(6) = e-128
Identities = 33/39 (84%), Positives = 36/39 (92%)
Frame = -3 / +2
Query: 429 QVLNDFQENPRAAQEHLKNPGVMQKIQKLVSAGIVQMR* 313
QVL DFQENP+AAQEH KNPGVM KIQKL+SAGIVQM+*
Sbjct: 17933 QVLVDFQENPKAAQEHTKNPGVMDKIQKLISAGIVQMK* 18049
Score = 68.9 bits (144), Expect(5) = 1e-065
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = +2 / -3
Query: 314 HLIWTIPALTSF*IFCITPGFFRCSWAALGFSWKSFSTCL 433
+ IWTIPAL SF I ITPGF CS AA GFSWKS +TC+
Sbjct: 18048 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTCI 17929
Score = 67.5 bits (141), Expect(6) = e-128
Identities = 23/30 (76%), Positives = 30/30 (100%)
Frame = -3 / +3
Query: 864 GNEFFKEQKYPEAIKHYTEALKRNPKDPRV 775
GNE+FK+QKYPEAIKHYTE++KRNP++P+V
Sbjct: 17067 GNEYFKQQKYPEAIKHYTESIKRNPQNPKV 17156
Score = 52.8 bits (109), Expect(2) = 1e-030
Identities = 19/26 (73%), Positives = 24/26 (92%)
Frame = -3 / +1
Query: 1146 ECIKDCDKAVERGRELRADFKMISRA 1069
+CIKDCD+ VE GRELR+D+KMI+RA
Sbjct: 7348 DCIKDCDRVVEGGRELRSDYKMIARA 7425
Score = 45.1 bits (92), Expect(6) = e-128
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = -3 / +2
Query: 489 QNKAMQDPEIQNILTDPIMRQV 424
Q KAM DPEIQNIL DP+MRQV
Sbjct: 17786 QAKAMSDPEIQNILQDPVMRQV 17851
Score = 37.3 bits (75), Expect(5) = 1e-065
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +2 / -2
Query: 809 SV*CFIASGYFCSLKNSLP 865
SV*C IASGYFC LK S P
Sbjct: 17122 SV*CLIASGYFCCLKYSFP 17066
Score = 36.8 bits (74), Expect(6) = e-128
Identities = 11/22 (50%), Positives = 20/22 (90%)
Frame = -3 / +1
Query: 552 CIEQINKANRGEISQDELQERQ 487
C++QIN+ +RG+++ +EL+ERQ
Sbjct: 17560 CVKQINRTSRGDVTPEELKERQ 17625
Score = 32.2 bits (64), Expect(5) = 1e-065
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +1 / -2
Query: 424 HLSHDRVGKNVLDLWVLHSFVLSFLQ 501
+LSH+RV K+VL+LW+ H L +Q
Sbjct: 17851 NLSHNRVLKDVLNLWI*HCLSLRNIQ 17774
>gb|CX529733.1|CX529733 s13dNF52B09MJ074_245512 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 336 bits (729), Expect = 3e-091
Identities = 133/157 (84%), Positives = 150/157 (95%)
Frame = -3 / +3
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 141 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 320
Query: 969 NPDTLKKLNEAERAKKELEQQEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNP 790
NPDTLKKLNEAE+AKKELEQQEY+DP LADEEREKGNE+FK+QKYPEAIKHYTE++KRNP
Sbjct: 321 NPDTLKKLNEAEKAKKELEQQEYFDPNLADEEREKGNEYFKQQKYPEAIKHYTESIKRNP 500
Query: 789 KDPRVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFT 679
++P+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFT
Sbjct: 501 QNPKAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFT 611
Score = 143 bits (306), Expect(2) = 5e-040
Identities = 73/114 (64%), Positives = 74/114 (64%)
Frame = +2 / -2
Query: 809 SV*CFIASGYFCSLKNSLPFSLSSSANFGS*YXXXXXXXXXXXXXXXXXRVSGFRCSVRA 988
SV*C IASGYFC LK S PFSLSSSA FGS Y RVSG RCSV A
Sbjct: 481 SV*CLIASGYFCCLKYSFPFSLSSSAKFGSKYSCCSSSFLAFSASLSFFRVSGLRCSVSA 302
Query: 989 FW*VSMAISKSLEVLASLTRAVPFLVSALEIILKSARSSLPLSTALSQSLMHSS 1150
FW*VSM S SLE A L AVPFLV AL IIL SA +S PLSTALSQS M SS
Sbjct: 301 FW*VSMTGS*SLEHFAVLPMAVPFLVKALAIIL*SALNSRPLSTALSQSFMQSS 140
Score = 43.7 bits (89), Expect(2) = 5e-040
Identities = 22/50 (44%), Positives = 27/50 (54%)
Frame = +1 / -1
Query: 682 EGGV*FKTLLCIFKTFRHGS*LGVAGSPIAVNPRILWVSLESLRVVFYCF 831
EG + TLLCI TFRH V S + ++ RILWVS L V+ F
Sbjct: 608 EGRIKLNTLLCIL*TFRHCPQFCVTCSSVTISLRILWVSFYRLCVMLNSF 459
>gb|BQ154941.1|BQ154941 NF074E06IR1F1051 Irradiated Medicago truncatula cDNA clone
NF074E06IR 5', mRNA sequence
Length = 631
Score = 214 bits (462), Expect(2) = 1e-082
Identities = 85/114 (74%), Positives = 102/114 (89%)
Frame = -3 / +3
Query: 654 IQFFMKEYDKAMETYQAGLKHDPKNQELLDGVRRCIEQINKANRGEISQDELQERQNKAM 475
+QFFMKEY+KA+ETYQ GLKHD NQELL+GVR C++QIN+ +RG+++ +EL+ERQ KAM
Sbjct: 147 VQFFMKEYEKALETYQEGLKHDANNQELLEGVRSCVKQINRTSRGDVTPEELKERQAKAM 326
Query: 474 QDPEIQNILTDPIMRQVLNDFQENPRAAQEHLKNPGVMQKIQKLVSAGIVQMR* 313
DPEIQNIL DP+MRQVL DFQENP+AAQEH KNPGVM KIQKL+SAGIVQM+*
Sbjct: 327 SDPEIQNILQDPVMRQVLVDFQENPKAAQEHTKNPGVMDKIQKLISAGIVQMK* 488
Score = 146 bits (313), Expect(2) = 4e-044
Identities = 68/113 (60%), Positives = 73/113 (64%)
Frame = +2 / -1
Query: 314 HLIWTIPALTSF*IFCITPGFFRCSWAALGFSWKSFSTCLMIGSVRMFWISGSCIALFCL 493
+ IWTIPAL SF I ITPGF CS AA GFSWKS +TCL+ GS RMF ISGS IAL CL
Sbjct: 487 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTCLITGS*RMF*ISGSDIALACL 308
Query: 494 SCNXXXXXXXXXXXXXXXMHLLTPSSSSWFFGSCFRPA**VSMALSYSFMKN* 652
S N L TPSS+SW F SCF P+* VS A SYSFMKN*
Sbjct: 307 SFNSSGVTSPRLVLLICFTQLRTPSSNSWLFASCFNPS*YVSKAFSYSFMKN* 149
Score = 114 bits (244), Expect(2) = 1e-082
Identities = 44/52 (84%), Positives = 48/52 (92%)
Frame = -3 / +2
Query: 798 RNPKDPRVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFF 643
RNP++P+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFTKGYTRKGA F
Sbjct: 2 RNPQNPKAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFTKGYTRKGAGSVF 157
Score = 111 bits (238), Expect(2) = 1e-031
Identities = 52/112 (46%), Positives = 71/112 (63%)
Frame = +1 / -3
Query: 319 HLDYPSTYKLLNLLHNSRVLQVLLGRPWVLLEII*HLSHDRVGKNVLDLWVLHSFVLSFL 498
HLD T +LL+L+H++RVL + L + LEI +LSH+RV K+VL+LW+ H LS L
Sbjct: 482 HLDNSGTDQLLDLVHHTRVLGMFLCSLRIFLEINQYLSHNRVLKDVLNLWI*HCLSLSLL 303
Query: 499 QFVLTYFTSVSLVNLFNAPPNTIKQFLVFRVMLQASLIGFHGLVIFFHEELN 654
Q +Y ++ +NL + N KQFLV +M Q LI F L IFFHE+LN
Sbjct: 302 QLFRSYISTTGSINLLHTTSNPFKQFLVVCIMFQPFLICFQSLFIFFHEKLN 147
Score = 54.2 bits (112), Expect(2) = 4e-044
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = +2 / -2
Query: 656 APFLVYPLVKVGSSSRHFSASLRPSGMAPN 745
APF V PLVKVGSSS HFSAS +PSG+AP+
Sbjct: 144 APFRV*PLVKVGSSSIHFSASFKPSGIAPS 55
Score = 46.9 bits (96), Expect(2) = 1e-031
Identities = 21/47 (44%), Positives = 26/47 (55%)
Frame = +1 / -1
Query: 658 TFPCVPFSEGGV*FKTLLCIFKTFRHGS*LGVAGSPIAVNPRILWVS 798
T P + EG + TLLCI TFRH V S + ++ RILWVS
Sbjct: 142 TLPSITLGEGRIKLNTLLCIL*TFRHCPQFCVTCSSVTISLRILWVS 2
>gb|CX533112.1|CX533112 s13dNF0CA03MJ017_319389 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 657
Score = 184 bits (396), Expect = 3e-045
Identities = 75/90 (83%), Positives = 84/90 (93%)
Frame = -3 / +3
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 387 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 566
Query: 969 NPDTLKKLNEAERAKKELEQQEYYDPKLAD 880
NPDTLKKLNEAE+AKKELEQQEY+DP LAD
Sbjct: 567 NPDTLKKLNEAEKAKKELEQQEYFDPNLAD 656
>emb|CR479179.1| mth2-189M5RM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 405
Score = 160 bits (344), Expect = 4e-038
Identities = 66/79 (83%), Positives = 74/79 (93%)
Frame = -3 / +1
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 169 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 348
Query: 969 NPDTLKKLNEAERAKKELE 913
NPDTLKKLNEAE+AKKELE
Sbjct: 349 NPDTLKKLNEAEKAKKELE 405
>emb|CR479743.1| mth2-190I5FM1 BAC end, cultivar Jemalong A17 of Medicago truncatula,
genomic survey sequence
Length = 379
Score = 140 bits (300), Expect = 5e-032
Identities = 57/69 (82%), Positives = 64/69 (92%)
Frame = -3 / +3
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 171 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 350
Query: 969 NPDTLKKLN 943
NPDTLKKLN
Sbjct: 351 NPDTLKKLN 377
>gb|AW694998.1|AW694998 NF082D04ST1F1041 Developing stem Medicago truncatula cDNA clone
NF082D04ST 5', mRNA sequence
Length = 666
Score = 112 bits (239), Expect = 1e-023
Identities = 46/117 (39%), Positives = 71/117 (60%)
Frame = -3 / +2
Query: 909 QEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMP 730
Q +D +ADE + KGN F + AI+H++EA+ +P + +YSNR+A Y L
Sbjct: 62 QNPFDLSMADEAKAKGNAAFSSGDFSTAIRHFSEAIDLSPTNHVLYSNRSAAYASLQNYT 241
Query: 729 EGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETYQAGLKHDPKNQELLDGV 559
+ L DA+K +EL P ++KGY+R GA + +YD A+ Y+ GL+ DP N+ L G+
Sbjct: 242 DALTDAKKTVELKPDWSKGYSRLGAAHLGLSQYDDAVSAYKKGLEIDPNNEPLKSGL 412
>gb|AW191221.1|AW191221 T113396e KV2 Medicago truncatula cDNA clone pKV2-2B23, mRNA
sequence
Length = 428
Score = 108 bits (231), Expect = 2e-022
Identities = 46/118 (38%), Positives = 75/118 (63%)
Frame = -3 / -2
Query: 921 ELEQQEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKL 742
EL+ + Y +++GNEFFK KY +A YT+A+K++P +P ++SNRAA +L
Sbjct: 382 ELDPKGY*RTSCEISLKDQGNEFFKSGKYLKAAALYTQAIKKDPSNPTLFSNRAAALLQL 203
Query: 741 GAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETYQAGLKHDPKNQELL 568
+ + L DAE ++L P + KG+ RKG I MK YD A+ ++Q +++P++QE+L
Sbjct: 202 DKLNKALDDAEMTIKLKPEWEKGHFRKGCILEAMKRYDDALASFQIASQYNPQSQEVL 29
>gb|AW191214.1|AW191214 T113386e KV2 Medicago truncatula cDNA clone pKV2-2A22, mRNA
sequence
Length = 415
Score = 106 bits (227), Expect = 5e-022
Identities = 45/118 (38%), Positives = 75/118 (63%)
Frame = -3 / -2
Query: 921 ELEQQEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKL 742
EL+ + Y +++GNEFFK KY +A YT+A+K++P +P ++SNRAA +L
Sbjct: 381 ELDPKGY*RTSCEISLKDQGNEFFKSGKYLKAAALYTQAIKKDPSNPTLFSNRAAALLQL 202
Query: 741 GAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETYQAGLKHDPKNQELL 568
+ + L DAE ++L P + KG+ RKG I MK YD A+ ++Q +++P++Q++L
Sbjct: 201 DKLNKALDDAEMTIKLKPEWEKGHFRKGCILEAMKRYDDALASFQIASQYNPQSQKVL 28
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 1,091,842,439
Number of Sequences: 392609
Number of extensions: 15330932
Number of successful extensions: 53858
Number of sequences better than 1.0e-020: 10
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 41213474208
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)