TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404928.2.1
(932 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|CT573076.1| Medicago truncatula chromosome 5 clone mth2... 171 2e-085
gb|BQ154941.1|BQ154941 NF074E06IR1F1051 Irradiated Medicago... 217 1e-083
gb|CX529733.1|CX529733 s13dNF52B09MJ074_245512 Methyl Jasmo... 170 7e-045
gb|AW694998.1|AW694998 NF082D04ST1F1041 Developing stem Med... 115 1e-024
gb|AW191221.1|AW191221 T113396e KV2 Medicago truncatula cDN... 108 2e-022
gb|AW191214.1|AW191214 T113386e KV2 Medicago truncatula cDN... 106 6e-022
gb|AA660354.1|AA660354 00225 MtRHE Medicago truncatula cDNA... 104 3e-021
>emb|CT573076.1| Medicago truncatula chromosome 5 clone mth2-181d5, COMPLETE SEQUENCE
Length = 89591
Score = 171 bits (368), Expect(6) = 2e-085
Identities = 66/78 (84%), Positives = 74/78 (94%)
Frame = -1 / +2
Query: 665 RVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAVETYQAG 486
+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFTKGYTRKGA+QFFMKEY+KA+ETYQ G
Sbjct: 17234 QAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFTKGYTRKGAVQFFMKEYEKALETYQEG 17413
Query: 485 LKHDPNNQELLDGVKRCV 432
LKHD NNQELL+GV+R +
Sbjct: 17414 LKHDANNQELLEGVRRYI 17467
Score = 115 bits (246), Expect = 1e-024
Identities = 47/114 (41%), Positives = 71/114 (62%)
Frame = -1 / +2
Query: 785 YDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEGL 606
+D +ADE + KGN F + AI+H++EA+ +P + +YSNR+A Y L + L
Sbjct: 13952 FDLSMADEAKAKGNAAFSSGDFSTAIRHFSEAIDLSPTNHVLYSNRSAAYASLQNYTDAL 14131
Query: 605 KDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGV 444
DA+K +EL P ++KGY+R GA + +YD AV Y+ GL+ DPNN+ L G+
Sbjct: 14132 TDAKKTVELKPDWSKGYSRLGAAHLGLSQYDDAVSAYKKGLEIDPNNEPLKSGL 14293
Score = 107 bits (229), Expect(6) = 2e-042
Identities = 47/65 (72%), Positives = 53/65 (81%)
Frame = +1 / -3
Query: 436 HLLTPSSSSWLFGSCFKPAW*VSTALSYSFMKN*IAPFLVYPLVKVGSSSRHFSASLRPS 615
+L TPSS+SWLF SCF P+ VS A SYSFMKN* APF V PLVKVGSSS HFSAS +PS
Sbjct: 17463 YLRTPSSNSWLFASCFNPS*YVSKAFSYSFMKN*TAPFRV*PLVKVGSSSIHFSASFKPS 17284
Query: 616 GMAPN 630
G+AP+
Sbjct: 17283 GIAPS 17269
Score = 80.8 bits (170), Expect(6) = 2e-085
Identities = 33/39 (84%), Positives = 36/39 (92%)
Frame = -1 / +2
Query: 314 QVLIDFQENPSAAQEHLKNPGVMQKIQKLVSAGIVQMR* 198
QVL+DFQENP AAQEH KNPGVM KIQKL+SAGIVQM+*
Sbjct: 17933 QVLVDFQENPKAAQEHTKNPGVMDKIQKLISAGIVQMK* 18049
Score = 67.5 bits (141), Expect(6) = 2e-085
Identities = 23/30 (76%), Positives = 30/30 (100%)
Frame = -1 / +3
Query: 749 GNEFFKEQKYPEAIKHYTEALKRNPKDPRV 660
GNE+FK+QKYPEAIKHYTE++KRNP++P+V
Sbjct: 17067 GNEYFKQQKYPEAIKHYTESIKRNPQNPKV 17156
Score = 61.1 bits (127), Expect(6) = 2e-042
Identities = 26/39 (66%), Positives = 27/39 (69%)
Frame = +1 / -3
Query: 199 HLI*TIPALTSF*IFCITPGFFRCSWAALGFSWKSIRTC 315
+ I TIPAL SF I ITPGF CS AA GFSWKS TC
Sbjct: 18048 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTC 17932
Score = 48.7 bits (100), Expect(6) = 2e-085
Identities = 26/57 (45%), Positives = 36/57 (63%)
Frame = -3 / +1
Query: 906 RI*YCYRXLPEGSDXASEPRNS*KVX*G*ESKERVRATRVL*SKIGR*GERERQRVL 736
R+* C+ LP+ + A PR+ + * *E +ER R TR+L SK G *GER+R +L
Sbjct: 16774 RL*TCH*DLPKSAYRAP*PRHPEETQ*S*EGQERTRTTRILRSKFG**GERKRYYLL 16944
Score = 45.1 bits (92), Expect(6) = 2e-085
Identities = 18/22 (81%), Positives = 19/22 (86%)
Frame = -1 / +2
Query: 374 QNKAMQDPEIQNILTDPIMRQV 309
Q KAM DPEIQNIL DP+MRQV
Sbjct: 17786 QAKAMSDPEIQNILQDPVMRQV 17851
Score = 37.3 bits (75), Expect(6) = 2e-042
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +1 / -2
Query: 694 SV*CFIASGYFCSLKNSLP 750
SV*C IASGYFC LK S P
Sbjct: 17122 SV*CLIASGYFCCLKYSFP 17066
Score = 37.3 bits (75), Expect(6) = 2e-085
Identities = 12/22 (54%), Positives = 20/22 (90%)
Frame = -1 / +1
Query: 437 CVEQINKANRGEISQDELQERQ 372
CV+QIN+ +RG+++ +EL+ERQ
Sbjct: 17560 CVKQINRTSRGDVTPEELKERQ 17625
Score = 34.5 bits (69), Expect(6) = 2e-042
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +3 / -2
Query: 309 NLSHDRVGKNVLDLWVLHSFVLSFLQ 386
NLSH+RV K+VL+LW+ H L +Q
Sbjct: 17851 NLSHNRVLKDVLNLWI*HCLSLRNIQ 17774
Score = 34.1 bits (68), Expect(6) = 2e-042
Identities = 15/23 (65%), Positives = 16/23 (69%)
Frame = +1 / -1
Query: 373 CLSCNSSWLISPLLALLICSTHL 441
CLS NSS + SP L LLIC T L
Sbjct: 17624 CLSFNSSGVTSPRLVLLICFTQL 17556
Score = 29.0 bits (57), Expect(6) = 2e-042
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = +1 / -3
Query: 841 RVSGFRCXVRAFW 879
RVSG RC V AFW
Sbjct: 16839 RVSGLRCSVSAFW 16801
>gb|BQ154941.1|BQ154941 NF074E06IR1F1051 Irradiated Medicago truncatula cDNA clone
NF074E06IR 5', mRNA sequence
Length = 631
Score = 217 bits (469), Expect(2) = 1e-083
Identities = 86/114 (75%), Positives = 103/114 (90%)
Frame = -1 / +3
Query: 539 IQFFMKEYDKAVETYQAGLKHDPNNQELLDGVKRCVEQINKANRGEISQDELQERQNKAM 360
+QFFMKEY+KA+ETYQ GLKHD NNQELL+GV+ CV+QIN+ +RG+++ +EL+ERQ KAM
Sbjct: 147 VQFFMKEYEKALETYQEGLKHDANNQELLEGVRSCVKQINRTSRGDVTPEELKERQAKAM 326
Query: 359 QDPEIQNILTDPIMRQVLIDFQENPSAAQEHLKNPGVMQKIQKLVSAGIVQMR* 198
DPEIQNIL DP+MRQVL+DFQENP AAQEH KNPGVM KIQKL+SAGIVQM+*
Sbjct: 327 SDPEIQNILQDPVMRQVLVDFQENPKAAQEHTKNPGVMDKIQKLISAGIVQMK* 488
Score = 162 bits (348), Expect(2) = 5e-049
Identities = 76/113 (67%), Positives = 81/113 (71%)
Frame = +1 / -1
Query: 199 HLI*TIPALTSF*IFCITPGFFRCSWAALGFSWKSIRTCRMIGSVRMFWISGSCIALFCL 378
+ I TIPAL SF I ITPGF CS AA GFSWKS TC + GS RMF ISGS IAL CL
Sbjct: 487 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTCLITGS*RMF*ISGSDIALACL 308
Query: 379 SCNSSWLISPLLALLICSTHLLTPSSSSWLFGSCFKPAW*VSTALSYSFMKN* 537
S NSS + SP L LLIC T L TPSS+SWLF SCF P+ VS A SYSFMKN*
Sbjct: 307 SFNSSGVTSPRLVLLICFTQLRTPSSNSWLFASCFNPS*YVSKAFSYSFMKN* 149
Score = 114 bits (244), Expect(2) = 1e-083
Identities = 44/52 (84%), Positives = 48/52 (92%)
Frame = -1 / +2
Query: 683 RNPKDPRVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFF 528
RNP++P+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFTKGYTRKGA F
Sbjct: 2 RNPQNPKAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFTKGYTRKGAGSVF 157
Score = 109 bits (232), Expect(2) = 4e-030
Identities = 51/112 (45%), Positives = 71/112 (63%)
Frame = +3 / -3
Query: 204 HLNYPSTYKLLNLLHNPGVLQVLLGRTWVLLEIY*NLSHDRVGKNVLDLWVLHSFVLSFL 383
HL+ T +LL+L+H+ VL + L + LEI LSH+RV K+VL+LW+ H LS L
Sbjct: 482 HLDNSGTDQLLDLVHHTRVLGMFLCSLRIFLEINQYLSHNRVLKDVLNLWI*HCLSLSLL 303
Query: 384 QFVLAYFTSVGLVDLFNTPLNTIKQFLVVRVMLQASLVGFHGLVIFFHEELN 539
Q +Y ++ G ++L +T N KQFLVV +M Q L+ F L IFFHE+LN
Sbjct: 302 QLFRSYISTTGSINLLHTTSNPFKQFLVVCIMFQPFLICFQSLFIFFHEKLN 147
Score = 54.2 bits (112), Expect(2) = 5e-049
Identities = 23/30 (76%), Positives = 26/30 (86%)
Frame = +1 / -2
Query: 541 APFLVYPLVKVGSSSRHFSASLRPSGMAPN 630
APF V PLVKVGSSS HFSAS +PSG+AP+
Sbjct: 144 APFRV*PLVKVGSSSIHFSASFKPSGIAPS 55
Score = 44.1 bits (90), Expect(2) = 4e-030
Identities = 21/47 (44%), Positives = 25/47 (53%)
Frame = +3 / -1
Query: 543 TFPRIPFGEGGVKFKTLFCVFKTFRHGS*LGVAGGPIAINPRILRVS 683
T P I GEG +K TL C+ TFRH V + I+ RIL VS
Sbjct: 142 TLPSITLGEGRIKLNTLLCIL*TFRHCPQFCVTCSSVTISLRILWVS 2
>gb|CX529733.1|CX529733 s13dNF52B09MJ074_245512 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 611
Score = 170 bits (366), Expect(2) = 7e-045
Identities = 63/75 (84%), Positives = 73/75 (97%)
Frame = -1 / +3
Query: 788 FYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEG 609
++DP LADEEREKGNE+FK+QKYPEAIKHYTE++KRNP++P+ YSNRAACYTKLGAMPEG
Sbjct: 387 YFDPNLADEEREKGNEYFKQQKYPEAIKHYTESIKRNPQNPKAYSNRAACYTKLGAMPEG 566
Query: 608 LKDAEKCLELDPTFT 564
LKDAEKC+ELDPTFT
Sbjct: 567 LKDAEKCIELDPTFT 611
Score = 32.2 bits (64), Expect(2) = 7e-045
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -1 / +3
Query: 902 FDIAIEXSQKALTXHRNPET 843
++ IE QKALT HRNP+T
Sbjct: 273 YEPVIETYQKALTEHRNPDT 332
>gb|AW694998.1|AW694998 NF082D04ST1F1041 Developing stem Medicago truncatula cDNA clone
NF082D04ST 5', mRNA sequence
Length = 666
Score = 115 bits (246), Expect = 1e-024
Identities = 47/114 (41%), Positives = 71/114 (62%)
Frame = -1 / +2
Query: 785 YDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEGL 606
+D +ADE + KGN F + AI+H++EA+ +P + +YSNR+A Y L + L
Sbjct: 71 FDLSMADEAKAKGNAAFSSGDFSTAIRHFSEAIDLSPTNHVLYSNRSAAYASLQNYTDAL 250
Query: 605 KDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGV 444
DA+K +EL P ++KGY+R GA + +YD AV Y+ GL+ DPNN+ L G+
Sbjct: 251 TDAKKTVELKPDWSKGYSRLGAAHLGLSQYDDAVSAYKKGLEIDPNNEPLKSGL 412
>gb|AW191221.1|AW191221 T113396e KV2 Medicago truncatula cDNA clone pKV2-2B23, mRNA
sequence
Length = 428
Score = 108 bits (230), Expect = 2e-022
Identities = 44/107 (41%), Positives = 71/107 (66%)
Frame = -1 / -2
Query: 758 REKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEGLKDAEKCLEL 579
+++GNEFFK KY +A YT+A+K++P +P ++SNRAA +L + + L DAE ++L
Sbjct: 334 KDQGNEFFKSGKYLKAAALYTQAIKKDPSNPTLFSNRAAALLQLDKLNKALDDAEMTIKL 155
Query: 578 DPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGVKR 438
P + KG+ RKG I MK YD A+ ++Q +++P +QE+L K+
Sbjct: 154 KPEWEKGHFRKGCILEAMKRYDDALASFQIASQYNPQSQEVLKKKKK 14
>gb|AW191214.1|AW191214 T113386e KV2 Medicago truncatula cDNA clone pKV2-2A22, mRNA
sequence
Length = 415
Score = 106 bits (226), Expect = 6e-022
Identities = 43/107 (40%), Positives = 71/107 (66%)
Frame = -1 / -2
Query: 758 REKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEGLKDAEKCLEL 579
+++GNEFFK KY +A YT+A+K++P +P ++SNRAA +L + + L DAE ++L
Sbjct: 333 KDQGNEFFKSGKYLKAAALYTQAIKKDPSNPTLFSNRAAALLQLDKLNKALDDAEMTIKL 154
Query: 578 DPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGVKR 438
P + KG+ RKG I MK YD A+ ++Q +++P +Q++L K+
Sbjct: 153 KPEWEKGHFRKGCILEAMKRYDDALASFQIASQYNPQSQKVLKKKKK 13
>gb|AA660354.1|AA660354 00225 MtRHE Medicago truncatula cDNA 5' similar to stress
inducible; heat shock protein, mRNA sequence
Length = 590
Score = 104 bits (221), Expect = 3e-021
Identities = 44/113 (38%), Positives = 65/113 (57%)
Frame = -1 / +3
Query: 782 DPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEGLK 603
D +A E + KG+ F + AI H++EA+ +P + +YSNR+A Y L + L
Sbjct: 51 DLSMAXEAKAKGDAAFSSGXFSTAIXHFSEAIDXSPTNHVLYSNRSAAYASLXNYTDALT 230
Query: 602 DAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGV 444
DA K + L P ++KGY+R GA + +YD AV Y GL+ DPNN+ L G+
Sbjct: 231 DANKTVXLKPDWSKGYSRLGAAHLGLSQYDDAVSAYXKGLEIDPNNEPLKSGL 389
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 867,522,308
Number of Sequences: 392609
Number of extensions: 12688621
Number of successful extensions: 54017
Number of sequences better than 1.0e-020: 9
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 32040963180
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)