TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS9f07.yg.2.1
(394 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY082444.1| Medicago sativa clone MS1-3FTa alpha 1,3 fuc... 224 4e-060
gb|AY082445.1| Medicago sativa clone MS1-3FTb alpha 1,3 fuc... 224 4e-060
gb|CO511798.1|CO511798 s13dSG02B0800073_103496 Glandular tr... 28 0.59
gb|L40327.1|ALFASPSYN Medicago sativa asparagine synthetase... 27 1.1
gb|CO513120.1|CO513120 s13dSG23B0300025_129404 Glandular tr... 27 1.5
gb|AW698676.1|AW698676 R110 non-glandular-haired subtracted... 23 1.8
gb|CO513145.1|CO513145 s13dSG23D0600046_129454 Glandular tr... 26 2.1
gb|L39371.1|ALFPEPC Medicago sativa phosphoenolpyruvate car... 25 4.0
gb|CO515621.1|CO515621 s13dSG47G0300024_418301 Glandular tr... 25 5.4
gb|CO516504.1|CO516504 s13dSG28G1000082_446336 Glandular tr... 25 5.4
>gb|AY082444.1| Medicago sativa clone MS1-3FTa alpha 1,3 fucosyltransferase mRNA,
complete cds
Length = 2027
Score = 224 bits (484), Expect = 4e-060
Identities = 88/131 (67%), Positives = 106/131 (80%)
Frame = -1 / +1
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSSKKGTVYHLF 215
KY+GPSDSFKAL+DMAAVHSSCRLCIH+ATK KEE++P F RPC C+ TVYH++
Sbjct: 1243 KYEGPSDSFKALVDMAAVHSSCRLCIHLATKSREKEEKSPDFKKRPCKCTRGSETVYHIY 1422
Query: 214 IRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRI 35
+RERG F+ ESIY+RS LTL + ++AVL KF SLNHVPVWK ERP ++GGD LK+Y+I
Sbjct: 1423 VRERGTFEMESIYLRSSNLTLESFKTAVLTKFTSLNHVPVWKPERPEILKGGDKLKVYKI 1602
Query: 34 YPVGLTQRQAL 2
P GLTQRQAL
Sbjct: 1603 IPAGLTQRQAL 1635
Score = 70.3 bits (147), Expect(2) = 8e-022
Identities = 32/47 (68%), Positives = 37/47 (78%)
Frame = +3 / -1
Query: 252 LQGRFVNFGVLSSFK*ILVAICIQRRQDE*TAAMSIRALKESDGPSY 392
LQGRF+ G+ SSF + VA +QRRQDE TAA+S RALKESDGPSY
Sbjct: 1385 LQGRFLKSGLFSSFSLLFVAK*MQRRQDECTAAISTRALKESDGPSY 1245
Score = 62.5 bits (130), Expect = 3e-011
Identities = 40/119 (33%), Positives = 67/119 (56%)
Frame = -3 / +3
Query: 392 V*WSI*FFQSSYRHGSGSFILSSLYTYCYQDSFKGGKDSKIYKSTL*LFQQKGDSLPLVY 213
V* +I*FFQS +GS +FIL L++ +++ +G ++ ++ ++TL + + + + +
Sbjct: 1245 V*RAI*FFQSPCGYGSCTFILPPLHSLGHKE*REGREEPRLQEATLQVHSRVRNCISYLC 1424
Query: 212 PRERAV*VREHLYEIGPVNTGSLGICSAR*I*VPQPRSCMEG*KATEY*RWG*PETIQN 36
R+ +* HL EI N+ L C + + VP+ SCME *K RW * E +QN
Sbjct: 1425 ERKGNI*DGVHLLEI*QFNS*VLQDCCSYEVHVPESCSCMEA*KT*NSKRWR*IEGLQN 1601
Score = 56.5 bits (117), Expect = 2e-009
Identities = 41/130 (31%), Positives = 59/130 (45%)
Frame = -2 / +2
Query: 393 SMMVHLILSKLLSTWQRFIHLVVFVYILLPRFI*RRKGLQNLQIDLVAVPAKRGQFTTCL 214
SM HLILSK L WQ +IHL F + R R++ Q + DL + + + +
Sbjct: 1244 SMKGHLILSKPLWIWQLYIHLAAFAFTWPQRVERRKRRAQTSRSDLASALEGQKLYIISM 1423
Query: 213 SEREGGLSQRAFI*DRAS*HWEPWNLQCSVNLGPSTTFLYGRMKGHRVLEVGMT*NYTEF 34
E+ L F *D A * P L + P FLYG +K + +V + * +T+
Sbjct: 1424 *EKGEHLRWSPFT*DLAI*LLSPSRLLFLRSSRP*IMFLYGSLKDLKF*KVAIN*RFTK* 1603
Query: 33 TQSV*HNVRL 4
*H +L
Sbjct: 1604 YLRA*HRGKL 1633
Score = 47.8 bits (98), Expect(2) = 3e-014
Identities = 20/46 (43%), Positives = 25/46 (54%)
Frame = +2 / -3
Query: 257 RSICKFWSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTIIL 394
RS+ + W+ L L + K R*M CH+ K FERIRW IL
Sbjct: 1380 RSLLEVWALLFLLSTLCGQVNAKAAR*MYSCHIHKGFERIRWPFIL 1243
Score = 46.4 bits (95), Expect(2) = 8e-022
Identities = 28/65 (43%), Positives = 31/65 (47%)
Frame = +3 / -1
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPDLI*MLSDL 194
CV+P G IL +F FHTGT RD+N TA K V DL *M S
Sbjct: 1622 CVKPAGIIL*TFNLSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDLK*MDSIS 1443
Query: 195 NRPLS 209
N PLS
Sbjct: 1442 NVPLS 1428
Score = 43.2 bits (88), Expect(2) = 3e-014
Identities = 24/65 (36%), Positives = 35/65 (53%)
Frame = +2 / -3
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARSHIN 181
+SL LC V Q I T F LPYRN+++G + ++ +G + *+ARS +N
Sbjct: 1635 KSLPLCQARRYYFVNLQFIATF*NFRSFRLPYRNMIQGRELRKNSSLEGLKS*IARSQVN 1456
Query: 182 AL*LK 196
L LK
Sbjct: 1455 GLHLK 1441
Score = 26.3 bits (51), Expect(2) = 0.33
Identities = 14/24 (58%), Positives = 14/24 (58%)
Frame = +1 / -2
Query: 322 KDDKMNEPLPCR*EL*KNQMDHHT 393
K KMN LP L*KNQM HT
Sbjct: 1315 KGGKMNVQLPYPQGL*KNQMALHT 1244
Score = 22.1 bits (42), Expect(2) = 0.33
Identities = 13/28 (46%), Positives = 14/28 (50%)
Frame = +1 / -2
Query: 37 FCIVSGHPHL*YSVAFHPSIQERG*GT* 120
FC S + HL F SIQE GT*
Sbjct: 1600 FCKPSIYRHLLEFQVFQASIQEHDSGT* 1517
>gb|AY082445.1| Medicago sativa clone MS1-3FTb alpha 1,3 fucosyltransferase mRNA,
complete cds
Length = 2134
Score = 224 bits (484), Expect = 4e-060
Identities = 88/131 (67%), Positives = 106/131 (80%)
Frame = -1 / +1
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSSKKGTVYHLF 215
KY+GPSDSFKAL+DMAAVHSSCRLCIH+ATK KEE++P F RPC C+ TVYH++
Sbjct: 1243 KYEGPSDSFKALVDMAAVHSSCRLCIHLATKSREKEEKSPDFKKRPCKCTRGSETVYHIY 1422
Query: 214 IRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRI 35
+RERG F+ ESIY+RS LTL + ++AVL KF SLNHVPVWK ERP ++GGD LK+Y+I
Sbjct: 1423 VRERGTFEMESIYLRSSNLTLESFKTAVLTKFTSLNHVPVWKPERPQILKGGDKLKVYKI 1602
Query: 34 YPVGLTQRQAL 2
P GLTQRQAL
Sbjct: 1603 IPAGLTQRQAL 1635
Score = 70.3 bits (147), Expect(2) = 1e-021
Identities = 32/47 (68%), Positives = 37/47 (78%)
Frame = +3 / -3
Query: 252 LQGRFVNFGVLSSFK*ILVAICIQRRQDE*TAAMSIRALKESDGPSY 392
LQGRF+ G+ SSF + VA +QRRQDE TAA+S RALKESDGPSY
Sbjct: 1385 LQGRFLKSGLFSSFSLLFVAK*MQRRQDECTAAISTRALKESDGPSY 1245
Score = 69.3 bits (145), Expect = 2e-013
Identities = 41/119 (34%), Positives = 69/119 (57%)
Frame = -3 / +3
Query: 392 V*WSI*FFQSSYRHGSGSFILSSLYTYCYQDSFKGGKDSKIYKSTL*LFQQKGDSLPLVY 213
V* +I*FFQS +GS +FIL L++ +++ +G ++ ++ ++TL + + + + +
Sbjct: 1245 V*RAI*FFQSPCGYGSCTFILPPLHSLGHKE*REGREEPRLQEATLQVHSRVRNCISYLC 1424
Query: 212 PRERAV*VREHLYEIGPVNTGSLGICSAR*I*VPQPRSCMEG*KATEY*RWG*PETIQN 36
R+ +* HL EI N+G L C + + VP+ SCME *K + RW * E +QN
Sbjct: 1425 ERKGNI*DGVHLLEI*QFNSGVLQDCCSYEVHVPESCSCMEA*KTSNSKRWR*IEGLQN 1601
Score = 62.5 bits (130), Expect = 3e-011
Identities = 42/130 (32%), Positives = 60/130 (46%)
Frame = -2 / +2
Query: 393 SMMVHLILSKLLSTWQRFIHLVVFVYILLPRFI*RRKGLQNLQIDLVAVPAKRGQFTTCL 214
SM HLILSK L WQ +IHL F + R R++ Q + DL + + + +
Sbjct: 1244 SMKGHLILSKPLWIWQLYIHLAAFAFTWPQRVERRKRRAQTSRSDLASALEGQKLYIISM 1423
Query: 213 SEREGGLSQRAFI*DRAS*HWEPWNLQCSVNLGPSTTFLYGRMKGHRVLEVGMT*NYTEF 34
E+ L F *D A * W P L + P FLYG +K + +V + * +T+
Sbjct: 1424 *EKGEHLRWSPFT*DLAI*LWSPSRLLFLRSSRP*IMFLYGSLKDLKF*KVAIN*RFTK* 1603
Query: 33 TQSV*HNVRL 4
*H +L
Sbjct: 1604 YLRA*HRGKL 1633
Score = 47.8 bits (98), Expect(2) = 1e-014
Identities = 20/46 (43%), Positives = 25/46 (54%)
Frame = +2 / -2
Query: 257 RSICKFWSPFLL*MNLGSNMYTKTTR*MNRCHVDKSFERIRWTIIL 394
RS+ + W+ L L + K R*M CH+ K FERIRW IL
Sbjct: 1380 RSLLEVWALLFLLSTLCGQVNAKAAR*MYSCHIHKGFERIRWPFIL 1243
Score = 45.5 bits (93), Expect(2) = 1e-021
Identities = 25/65 (38%), Positives = 35/65 (53%)
Frame = +2 / -2
Query: 2 QSLTLC*TDWVNSV*FQVIPTSNTRWPFILPYRNVVEGPKFTEHCRFQGSQC*LARSHIN 181
+SL LC V Q I T F LPYRN+++G + ++ +G Q *+ARS +N
Sbjct: 1635 KSLPLCQARRYYFVNLQFIATF*NLRSFRLPYRNMIQGRELRKNSSLEGLQS*IARSQVN 1456
Query: 182 AL*LK 196
L LK
Sbjct: 1455 GLHLK 1441
Score = 44.6 bits (91), Expect(2) = 1e-014
Identities = 28/65 (43%), Positives = 31/65 (47%)
Frame = +3 / -3
Query: 15 CVRPTG*ILYSFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPSVNWPDLI*MLSDL 194
CV+P G IL +F FHTGT RD+N TA K V DL *M S
Sbjct: 1622 CVKPAGIIL*TFNLSPPFRI*GLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDLK*MDSIS 1443
Query: 195 NRPLS 209
N PLS
Sbjct: 1442 NVPLS 1428
Score = 26.3 bits (51), Expect(2) = 0.059
Identities = 14/24 (58%), Positives = 14/24 (58%)
Frame = +1 / -1
Query: 322 KDDKMNEPLPCR*EL*KNQMDHHT 393
K KMN LP L*KNQM HT
Sbjct: 1315 KGGKMNVQLPYPQGL*KNQMALHT 1244
Score = 24.9 bits (48), Expect(2) = 0.059
Identities = 20/48 (41%), Positives = 21/48 (43%)
Frame = +1 / -1
Query: 37 FCIVSGHPHL*YSVAFHPSIQERG*GT*IYRALQIPRLPVLTGPISYK 180
FC S + HL F SIQE GT* Q R P L IS K
Sbjct: 1600 FCKPSIYRHLLEFEVFQASIQEHDSGT*TS*EQQS*RTPELNC*ISSK 1457
>gb|CO511798.1|CO511798 s13dSG02B0800073_103496 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 616
Score = 28.1 bits (55), Expect = 0.59
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -2 / +2
Query: 351 WQRFIHLVVFVYILLPRFI*RRKGLQNL 268
WQ+ +HL ++ + L +F+ RRK L+ +
Sbjct: 140 WQQDLHLEGYLAVALGKFLVRRKKLRKM 223
>gb|L40327.1|ALFASPSYN Medicago sativa asparagine synthetase gene, exons 1-13 and complete
cds
Length = 7969
Score = 27.2 bits (53), Expect = 1.1
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2 / -1
Query: 135 QCSVNLGPSTTFLYGRMKGHRVL 67
+C ++GPSTTF Y G R L
Sbjct: 1066 ECGKDVGPSTTFYYMFNGGRRSL 998
>gb|CO513120.1|CO513120 s13dSG23B0300025_129404 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 26.7 bits (52), Expect = 1.5
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -1 / -1
Query: 259 PCSCSSKKGTVYHLFIRERG 200
PCSC+++K T F++E G
Sbjct: 136 PCSCNTRKKTKVLCFLKEVG 77
>gb|AW698676.1|AW698676 R110 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 565
Score = 22.6 bits (43), Expect(2) = 1.8
Identities = 8/19 (42%), Positives = 10/19 (52%)
Frame = +3 / +1
Query: 117 LNLPSTADSKAPSVNWPDL 173
LNL +D +NWP L
Sbjct: 505 LNLTRCSDQPQSKLNWPPL 561
Score = 20.3 bits (38), Expect(2) = 1.8
Identities = 6/13 (46%), Positives = 8/13 (61%)
Frame = +2 / +2
Query: 62 TSNTRWPFILPYR 100
+ N WP +LP R
Sbjct: 233 SENQLWPHLLPIR 271
>gb|CO513145.1|CO513145 s13dSG23D0600046_129454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 594
Score = 26.3 bits (51), Expect = 2.1
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = -1 / -2
Query: 310 ATKIHLKEERTPKFTNRPCSCSSKK 236
ATKI L+ + +R CSCS+++
Sbjct: 479 ATKIQLETQLKTNLFHRHCSCSAQQ 405
>gb|L39371.1|ALFPEPC Medicago sativa phosphoenolpyruvate carboxylase (PEPC) gene, complete
cds
Length = 7490
Score = 25.4 bits (49), Expect = 4.0
Identities = 7/18 (38%), Positives = 12/18 (66%)
Frame = -2 / +2
Query: 363 LLSTWQRFIHLVVFVYIL 310
L+ W F HL +F+Y++
Sbjct: 6941 LVKFWLHFFHLYLFLYVM 6994
>gb|CO515621.1|CO515621 s13dSG47G0300024_418301 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 574
Score = 24.9 bits (48), Expect = 5.4
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = +1 / +3
Query: 271 ILESFPPLNESW*QYVYKD 327
I E+F P N SW VYK+
Sbjct: 282 ISETFLPKNPSWNPVVYKE 338
>gb|CO516504.1|CO516504 s13dSG28G1000082_446336 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 446
Score = 24.9 bits (48), Expect = 5.4
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -3 / +1
Query: 356 RHGSGSFILSSLYTYCYQDS 297
RHG+ SFI S L+ + Y S
Sbjct: 100 RHGNSSFIPSRLFHHTYSCS 159
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,453,770
Number of Sequences: 7669
Number of extensions: 42000
Number of successful extensions: 2685
Number of sequences better than 10.0: 13
length of database: 1,248,568
effective HSP length: 40
effective length of database: 941,808
effective search space used: 84762720
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)