TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH17d04.yg.2.1
(637 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY082444.1| Medicago sativa clone MS1-3FTa alpha 1,3 fuc... 340 1e-094
gb|AY082445.1| Medicago sativa clone MS1-3FTb alpha 1,3 fuc... 340 1e-094
>gb|AY082444.1| Medicago sativa clone MS1-3FTa alpha 1,3 fucosyltransferase mRNA,
complete cds
Length = 2027
Score = 340 bits (736), Expect = 1e-094
Identities = 134/212 (63%), Positives = 164/212 (77%)
Frame = -1 / +1
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVAS 458
RY+FS FE E DY EKFFQ V G+IPVVVG PNIQ+F P + L+I L+DV S
Sbjct: 1000 RYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGPPNIQDFAPSPDSFLYIKELEDVES 1179
Query: 457 VAKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERT 278
VAK+M+ +A NP+A+N SLRWKY+GPSDSFKAL+DMAAVHSSCRLCIH+ATK KEE++
Sbjct: 1180 VAKSMRYLAENPEAYNHSLRWKYEGPSDSFKALVDMAAVHSSCRLCIHLATKSREKEEKS 1359
Query: 277 PKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVP 98
P F RPC C+ T+YH+++RERG F+ ESIY+RS LTL + ++AVL KF SLNHVP
Sbjct: 1360 PDFKKRPCKCTRGSETVYHIYVRERGTFEMESIYLRSSNLTLESFKTAVLTKFTSLNHVP 1539
Query: 97 VWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
VWK ERP ++GGD LK+Y+I P GLTQRQAL
Sbjct: 1540 VWKPERPEILKGGDKLKVYKIIPAGLTQRQAL 1635
Score = 159 bits (341), Expect(2) = 5e-049
Identities = 78/128 (60%), Positives = 88/128 (68%)
Frame = +3 / -1
Query: 252 LQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHLKD*LKASGFE 431
LQGRF+ G+ SSF + VA +QRRQDE TAA+S RALKESDGPSYFHL +*L ASGF
Sbjct: 1385 LQGRFLKSGLFSSFSLLFVAK*MQRRQDECTAAISTRALKESDGPSYFHLNE*LYASGFS 1206
Query: 432 AIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXTXX*KNFSXX*SXSXXX 611
A + + LAT++TSSS LI G S*IFG PTTTGI P T KNFS *S S
Sbjct: 1205 ARYLMDLATDSTSSSSLIYKNESGEGAKS*IFGGPTTTGIVPATSDWKNFSVT*SSSLEF 1026
Query: 612 SNXXLNL* 635
SN LNL*
Sbjct: 1025 SNAKLNL* 1002
Score = 49.6 bits (102), Expect(2) = 5e-049
Identities = 29/65 (44%), Positives = 31/65 (47%)
Frame = +3 / -1
Query: 15 CVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDL 194
CV+P G IL F FHTGT RD+N TA K RV DL *M S
Sbjct: 1622 CVKPAGIIL*TFNLSPPFRISGLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDLK*MDSIS 1443
Query: 195 NRPLS 209
N PLS
Sbjct: 1442 NVPLS 1428
>gb|AY082445.1| Medicago sativa clone MS1-3FTb alpha 1,3 fucosyltransferase mRNA,
complete cds
Length = 2134
Score = 340 bits (736), Expect = 1e-094
Identities = 134/212 (63%), Positives = 164/212 (77%)
Frame = -1 / +1
Query: 637 RYRFSXXFEXXXEXDYXXEKFFQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVAS 458
RY+FS FE E DY EKFFQ V G+IPVVVG PNIQ+F P + L+I L+DV S
Sbjct: 1000 RYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGPPNIQDFAPSPDSFLYIKELEDVES 1179
Query: 457 VAKTMKNIASNPDAFNQSLRWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERT 278
VAK+M+ +A NP+A+N SLRWKY+GPSDSFKAL+DMAAVHSSCRLCIH+ATK KEE++
Sbjct: 1180 VAKSMRYLAENPEAYNHSLRWKYEGPSDSFKALVDMAAVHSSCRLCIHLATKSREKEEKS 1359
Query: 277 PKFTNRPCSCSTKKGTIYHLFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVP 98
P F RPC C+ T+YH+++RERG F+ ESIY+RS LTL + ++AVL KF SLNHVP
Sbjct: 1360 PDFKKRPCKCTRGSETVYHIYVRERGTFEMESIYLRSSNLTLESFKTAVLTKFTSLNHVP 1539
Query: 97 VWKDERPPSIRGGDDLKLYRIYPVGLTQRQAL 2
VWK ERP ++GGD LK+Y+I P GLTQRQAL
Sbjct: 1540 VWKPERPQILKGGDKLKVYKIIPAGLTQRQAL 1635
Score = 159 bits (341), Expect(2) = 2e-048
Identities = 78/128 (60%), Positives = 88/128 (68%)
Frame = +3 / -3
Query: 252 LQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESDGPSYFHLKD*LKASGFE 431
LQGRF+ G+ SSF + VA +QRRQDE TAA+S RALKESDGPSYFHL +*L ASGF
Sbjct: 1385 LQGRFLKSGLFSSFSLLFVAK*MQRRQDECTAAISTRALKESDGPSYFHLNE*LYASGFS 1206
Query: 432 AIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGIEPXTXX*KNFSXX*SXSXXX 611
A + + LAT++TSSS LI G S*IFG PTTTGI P T KNFS *S S
Sbjct: 1205 ARYLMDLATDSTSSSSLIYKNESGEGAKS*IFGGPTTTGIVPATSDWKNFSVT*SSSLEF 1026
Query: 612 SNXXLNL* 635
SN LNL*
Sbjct: 1025 SNAKLNL* 1002
Score = 86.8 bits (183), Expect(2) = 6e-025
Identities = 48/127 (37%), Positives = 63/127 (49%)
Frame = +2 / -2
Query: 257 RTICKFWSPFLL*MDLGSNMYTKTTR*MNRCHVNKSFERIGWTIILPPQRLIEGIRV*SN 436
R++ + W+ L L + K R*M CH++K FERI W ILPPQ +I RV
Sbjct: 1380 RSLLEVWALLFLLSTLCGQVNAKAAR*MYSCHIHKGFERIRWPFILPPQ*MIICFRVFC* 1201
Query: 437 IFHCLSN*SNIIKLXNM*YCAFSRXKLLNIWSXXXXXXXXXXXXLKKLFXXIILLXXXLK 616
+ H L N NI+ ++* R K+LNIW L+KLF IILL K
Sbjct: 1200 VSHGLGNRLNIL*FFDI*K*IRRRSKILNIWRTNNHRDSSSNKRLEKLFSYIILLVRIFK 1021
Query: 617 XXAESVA 637
A+ VA
Sbjct: 1020 C*AKFVA 1000
Score = 47.8 bits (98), Expect(2) = 2e-048
Identities = 29/65 (44%), Positives = 31/65 (47%)
Frame = +3 / -3
Query: 15 CVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDL 194
CV+P G IL F FHTGT RD+N TA K RV DL *M S
Sbjct: 1622 CVKPAGIIL*TFNLSPPFRI*GLSGFHTGT*FRDVNFVRTAVLKDSRVKLLDLK*MDSIS 1443
Query: 195 NRPLS 209
N PLS
Sbjct: 1442 NVPLS 1428
Score = 41.4 bits (84), Expect(2) = 6e-025
Identities = 18/38 (47%), Positives = 26/38 (68%)
Frame = +2 / -2
Query: 83 FILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LK 196
F LPYRN+++G + ++ +G QS*+A S VN L LK
Sbjct: 1554 FRLPYRNMIQGRELRKNSSLEGLQS*IARSQVNGLHLK 1441
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,115,888
Number of Sequences: 7669
Number of extensions: 68694
Number of successful extensions: 946
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 156573430
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)