TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBL6b07.xg.2.1
(644 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 217 4e-057
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 82 2e-030
emb|AX776636.1| Sequence 5 from Patent WO02102841 77 7e-030
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 77 7e-030
emb|AX776635.1| Sequence 4 from Patent WO02102841 77 2e-027
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 77 2e-027
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 64 1e-023
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 63 3e-023
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 217 bits (468), Expect = 4e-057
Identities = 87/119 (73%), Positives = 103/119 (86%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLHAVEIIHRDVKTNNILLDNSFHVKVADFGLSRLYPPEVTH 196
L W R+ IA+ETAEALAYLHA +++HRDVK+NNILLD FHVKVADFGLSRL+P +VTH
Sbjct: 238 LPWSVRLDIALETAEALAYLHASDVMHRDVKSNNILLDEKFHVKVADFGLSRLFPNDVTH 417
Query: 197 VSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLANM 373
VST PQGTPGYVDP Y+QCY+LT+KSD YSFGVVLVEL+SS AVD+ R +++NLANM
Sbjct: 418 VSTAPQGTPGYVDPEYYQCYQLTDKSDAYSFGVVLVELISSLRAVDITRHRNDVNLANM 594
Score = 146 bits (314), Expect = 2e-036
Identities = 73/112 (65%), Positives = 78/112 (69%)
Frame = -3 / -1
Query: 375 AMLARLISLWLLPMSTAGLEDTSSTSTTPKLYTSLFSVSL*HWWYTGST*PGVPCGTVDT 196
AMLA+ SL L +STA +D SST TTPKL SL SVS *H Y+GST*PGVPCG VDT
Sbjct: 596 AMLAKFTSLRCLVISTARNDDISSTKTTPKL*ASLLSVSW*H**YSGST*PGVPCGAVDT 417
Query: 195 WVTSGGYRRDSPKSATFTWKLLSSSMLLVFTSRCMISTAWRYASASAVSMAM 40
VTS G + PKSAT TW S MLL TSRCM S AWR ASASAVS AM
Sbjct: 416 CVTSLGNNLERPKSATLTWNFSSRRMLLDLTSRCMTSDAWR*ASASAVSNAM 261
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 82.2 bits (173), Expect(4) = 2e-030
Identities = 35/82 (42%), Positives = 52/82 (63%)
Frame = +2 / +1
Query: 182 PEVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEIN 361
P TH+ST +GT GY+DP Y + Y+LTEKSDVYSFGV+LVE+++ + V+ + E
Sbjct: 1021 PGATHISTQVKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVEPKKKIDERV 1200
Query: 362 LANMALNRIQNHEVGQLVDPEL 427
A+ ++N + +DP L
Sbjct: 1201 TIRWAMKMLKNGDAVFAMDPRL 1266
Score = 46.0 bits (94), Expect(4) = 2e-030
Identities = 17/29 (58%), Positives = 22/29 (75%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRL 175
IIHRD+K +NIL+ + KVADFG +RL
Sbjct: 925 IIHRDIKASNILISENLRAKVADFGFARL 1011
Score = 27.6 bits (54), Expect(4) = 2e-030
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +2 / +1
Query: 455 KSIDLVAELAFRCLQLERDSRPSMXXVVEAL 547
K + V +LAF+CL SRP+M E L
Sbjct: 1288 KVVKKVFKLAFQCLAPSIHSRPAMKNCAEVL 1380
Score = 27.2 bits (53), Expect(4) = 2e-030
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2 / +1
Query: 14 GLTWPRRMSIAIETAEALAYLH 79
GL R+ IAI+ A A+ YLH
Sbjct: 841 GLEIGERLDIAIDIAHAITYLH 906
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 76.7 bits (161), Expect(3) = 7e-030
Identities = 33/77 (42%), Positives = 53/77 (68%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
++VS +GT GY+DP Y++ +L+EKSDV+SFGVVL+E+VS + +++ R E +L
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2681
Query: 371 MALNRIQNHEVGQLVDP 421
A I+ +V ++VDP
Sbjct: 2682 WAKPYIRASKVDEIVDP 2732
Score = 51.9 bits (107), Expect(3) = 7e-030
Identities = 21/33 (63%), Positives = 25/33 (75%)
Frame = +2 / +3
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
+IHRDVK++NILLD S KVADFG S+ P E
Sbjct: 2397 VIHRDVKSSNILLDQSMCAKVADFGFSKYAPQE 2495
Score = 35.0 bits (70), Expect(3) = 7e-030
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+SIA+ A LAYLH
Sbjct: 2316 LDWPTRLSIALGAARGLAYLH 2378
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 76.7 bits (161), Expect(3) = 7e-030
Identities = 33/77 (42%), Positives = 53/77 (68%)
Frame = +2 / +3
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
++VS +GT GY+DP Y++ +L+EKSDV+SFGVVL+E+VS + +++ R E +L
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2681
Query: 371 MALNRIQNHEVGQLVDP 421
A I+ +V ++VDP
Sbjct: 2682 WAKPYIRASKVDEIVDP 2732
Score = 51.9 bits (107), Expect(3) = 7e-030
Identities = 21/33 (63%), Positives = 25/33 (75%)
Frame = +2 / +3
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
+IHRDVK++NILLD S KVADFG S+ P E
Sbjct: 2397 VIHRDVKSSNILLDQSMCAKVADFGFSKYAPQE 2495
Score = 35.0 bits (70), Expect(3) = 7e-030
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +2 / +3
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+SIA+ A LAYLH
Sbjct: 2316 LDWPTRLSIALGAARGLAYLH 2378
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 76.7 bits (161), Expect(3) = 2e-027
Identities = 33/77 (42%), Positives = 53/77 (68%)
Frame = +2 / +1
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
++VS +GT GY+DP Y++ +L+EKSDV+SFGVVL+E+VS + +++ R E +L
Sbjct: 2521 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2700
Query: 371 MALNRIQNHEVGQLVDP 421
A I+ +V ++VDP
Sbjct: 2701 WAKPYIRASKVDEIVDP 2751
Score = 50.6 bits (104), Expect(3) = 2e-027
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
+IHRD+K++NILLD S KVADFG S+ P E
Sbjct: 2416 VIHRDLKSSNILLDQSMCAKVADFGFSKYAPQE 2514
Score = 27.6 bits (54), Expect(3) = 2e-027
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+SIA+ LA LH
Sbjct: 2335 LDWPTRLSIALGAPRGLA*LH 2397
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 76.7 bits (161), Expect(3) = 2e-027
Identities = 33/77 (42%), Positives = 53/77 (68%)
Frame = +2 / +1
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
++VS +GT GY+DP Y++ +L+EKSDV+SFGVVL+E+VS + +++ R E +L
Sbjct: 2500 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEWSLVE 2679
Query: 371 MALNRIQNHEVGQLVDP 421
A I+ +V ++VDP
Sbjct: 2680 WAKPYIRASKVDEIVDP 2730
Score = 50.6 bits (104), Expect(3) = 2e-027
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
+IHRD+K++NILLD S KVADFG S+ P E
Sbjct: 2395 VIHRDLKSSNILLDQSMCAKVADFGFSKYAPQE 2493
Score = 27.6 bits (54), Expect(3) = 2e-027
Identities = 11/21 (52%), Positives = 13/21 (61%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
L WP R+SIA+ LA LH
Sbjct: 2314 LDWPTRLSIALGAPRGLA*LH 2376
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 63.8 bits (133), Expect(4) = 1e-023
Identities = 30/62 (48%), Positives = 37/62 (59%)
Frame = +2 / +1
Query: 191 THVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAVDMGRSHSEINLAN 370
THVST GT GY P Y +LT KSD+YS GV L+EL++ + A+D R E NL
Sbjct: 238 THVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKEQNLVA 417
Query: 371 MA 376
A
Sbjct: 418 WA 423
Score = 43.7 bits (89), Expect(4) = 1e-023
Identities = 15/31 (48%), Positives = 25/31 (80%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYP 181
+I+RD+K +NILL +H K++DFGL+++ P
Sbjct: 133 VIYRDLKCSNILLGEDYHSKLSDFGLAKVGP 225
Score = 27.6 bits (54), Expect(4) = 1e-023
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +2 / +1
Query: 8 ERGLTWPRRMSIAIETAEALAYLH 79
++ L W RM IA A L YLH
Sbjct: 43 QKPLDWNTRMKIAAGAARGLEYLH 114
Score = 24.0 bits (46), Expect(4) = 1e-023
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +2 / +1
Query: 479 LAFRCLQLERDSRPSMXXVVEALN 550
+A C+Q + + RP + VV ALN
Sbjct: 520 IAAMCVQEQPNMRPVIADVVTALN 591
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 63.4 bits (132), Expect(3) = 3e-023
Identities = 26/49 (53%), Positives = 35/49 (71%)
Frame = +2 / +1
Query: 185 EVTHVSTVPQGTPGYVDPVYHQCYKLTEKSDVYSFGVVLVELVSSKPAV 331
E +H+S + GT GY+DP Y++ LT KSDVYSFGV L+EL+S A+
Sbjct: 463 EESHLSLLAAGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSGYKAI 609
Score = 51.0 bits (105), Expect(3) = 3e-023
Identities = 20/33 (60%), Positives = 27/33 (81%)
Frame = +2 / +1
Query: 89 IIHRDVKTNNILLDNSFHVKVADFGLSRLYPPE 187
IIHRD+KT+NILLD+ + KV+DFGLS + P +
Sbjct: 364 IIHRDIKTSNILLDSKWVAKVSDFGLSLMGPED 462
Score = 26.3 bits (51), Expect(3) = 3e-023
Identities = 7/21 (33%), Positives = 14/21 (66%)
Frame = +2 / +1
Query: 17 LTWPRRMSIAIETAEALAYLH 79
++W R+ +A++ A + YLH
Sbjct: 283 MSWNGRIKVALDAARGIEYLH 345
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,491,019
Number of Sequences: 7669
Number of extensions: 54489
Number of successful extensions: 975
Number of sequences better than 1.0e-020: 11
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 159352840
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)