TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAN24c09.yg.2.1
(425 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular tr... 160 9e-041
gb|DQ122795.1| Medicago sativa clone M22 putative cellulose... 97 5e-022
>gb|CO516105.1|CO516105 s13dSG65C1200098_445538 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 529
Score = 160 bits (344), Expect = 9e-041
Identities = 66/103 (64%), Positives = 78/103 (75%)
Frame = +1 / +1
Query: 100 FCLLWNV*TLCSLFHLLQLNNVASLWFMSLFICIFATSILXMRWSGVGIDDWWRNEQFWV 279
+C L + L F + +++N ASL FM+LFI I AT IL M+W GVGIDDWWRNEQFWV
Sbjct: 145 YCTLPAICLLTGKFIVPEISNYASLVFMALFISIAATGILEMQWGGVGIDDWWRNEQFWV 324
Query: 280 IGGVSSHLFAVFQGLLXVIAGVDTSXTXTSKGGDDDEFSELYL 408
IGG SSH FA+FQGLL V+AGVDT+ T TSK DD EFSELY+
Sbjct: 325 IGGASSHFFALFQGLLKVLAGVDTNFTVTSKAADDGEFSELYI 453
Score = 102 bits (217), Expect(2) = 8e-026
Identities = 43/69 (62%), Positives = 49/69 (71%)
Frame = -1 / -3
Query: 371 FDVXVXLVSTPAMTXRSP*NTAKRCEDTPPMTQNCSFLHQSSIPTPLHLIXRMLVAKMQI 192
FDV V VSTPA T P AK+C++ PP+TQNCSFLHQSSIPTP H I R+ VA M +
Sbjct: 416 FDVTVKFVSTPAKTLSKPWKRAKKCDEAPPITQNCSFLHQSSIPTPPHCISRIPVAAMDM 237
Query: 191 KSDMNHRLA 165
K MN RLA
Sbjct: 236 KRAMNTRLA 210
Score = 59.7 bits (124), Expect(3) = 2e-021
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +3 / +3
Query: 156 EQCCQPVVHVTFYLHFCYKHPXNEMEWCWD**LVEE*AVLGHWRCVL 296
+Q C+ VH ++H ++P + M WCW+ *LVEE* VLG WRC +
Sbjct: 201 KQLCKSCVHGPLHIHRSNRYP*DAMGWCWNR*LVEE*TVLGDWRCFI 341
Score = 40.5 bits (82), Expect(3) = 2e-021
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = +1 / +1
Query: 1 TLPAICLLTGKFITPEV 51
TLPAICLLTGKFI PE+
Sbjct: 151 TLPAICLLTGKFIVPEI 201
Score = 31.8 bits (63), Expect(3) = 2e-021
Identities = 15/25 (60%), Positives = 17/25 (68%)
Frame = +3 / +3
Query: 330 GHSWC*YKLHXDIKGWR**RILRAV 404
G SWC +KLH IK R* RIL A+
Sbjct: 375 GFSWCRHKLHCYIKSSR*WRILGAL 449
Score = 27.6 bits (54), Expect(2) = 8e-026
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1 / -3
Query: 47 SGVINFPVNKQMAG 6
SG INFPV + MAG
Sbjct: 197 SGTINFPVRRHMAG 156
>gb|DQ122795.1| Medicago sativa clone M22 putative cellulose synthase catalytic
subunit mRNA, partial cds
Length = 512
Score = 97.3 bits (206), Expect(2) = 5e-022
Identities = 39/97 (40%), Positives = 59/97 (60%)
Frame = +1 / +2
Query: 100 FCLLWNV*TLCSLFHLLQLNNVASLWFMSLFICIFATSILXMRWSGVGIDDWWRNEQFWV 279
+C L + F + L+ ++ + + + + ++L ++WSG+ + DWWRNEQFW+
Sbjct: 2 YCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHDWWRNEQFWL 181
Query: 280 IGGVSSHLFAVFQGLLXVIAGVDTSXTXTSKGGDDDE 390
IGG S+H AV QGLL VIAGVD S T TSK ++
Sbjct: 182 IGGTSAHPAAVLQGLLKVIAGVDISFTLTSKSATPED 292
Score = 19.9 bits (37), Expect(2) = 5e-022
Identities = 6/9 (66%), Positives = 9/9 (100%)
Frame = +1 / +2
Query: 382 DDEFSELYL 408
+DEF++LYL
Sbjct: 296 EDEFADLYL 322
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,710,731
Number of Sequences: 7669
Number of extensions: 49971
Number of successful extensions: 2707
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 40
effective length of database: 941,808
effective search space used: 95122608
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)