TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAF31c07.yg.2.1
         (352 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AW698589.1|AW698589  g555 glandular-haired subtracted cDN...    29   0.36 
gb|CO515817.1|CO515817  s13dSG54D0700060_421173 Glandular tr...    28   0.68 
gb|CO516929.1|CO516929  s13dSG58F0300027_468404 Glandular tr...    26   2.4  
gb|CO515258.1|CO515258  s13dSG96G0300024_399381 Glandular tr...    25   3.3  
gb|CO515099.1|CO515099  s13dSG40B0600057_399063 Glandular tr...    25   4.6  
gb|CO512234.1|CO512234  s13dSG03E1200087_113982 Glandular tr...    24   8.7  
gb|CO513025.1|CO513025  s13dSG09G0300020_121976 Glandular tr...    24   8.7  

>gb|AW698589.1|AW698589 g555 glandular-haired subtracted cDNA library Medicago sativa cDNA,
           mRNA sequence
          Length = 415

 Score = 28.6 bits (56), Expect = 0.36
 Identities = 9/21 (42%), Positives = 14/21 (66%)
 Frame = +3 / +3

                                
Query: 15  GSTSAGLHQILPWLYYHNVIG 77
           GS S GLHQ + W+++  + G
Sbjct: 315 GSXSRGLHQYVSWIFFAFMFG 377



>gb|CO515817.1|CO515817 s13dSG54D0700060_421173 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 538

 Score = 27.6 bits (54), Expect = 0.68
 Identities = 11/18 (61%), Positives = 12/18 (66%)
 Frame = -1 / +3

                             
Query: 121 GFAASPSTNRKKCETPMT 68
           GFA SPS + KK  TP T
Sbjct: 192 GFATSPSISLKKTRTPTT 245




 Score = 25.4 bits (49), Expect(2) = 2.2
 Identities = 11/31 (35%), Positives = 17/31 (54%)
 Frame = +3 / -1

                                          
Query: 69  VIGVSHFFLLVEGEAAKPAXTSVLESIQPHL 161
           V+GV  F   +EGE AKP    + + ++  L
Sbjct: 244 VVGVLVFLREMEGEVAKPGRRDLWKMVEEGL 152




 Score = 16.6 bits (30), Expect(2) = 2.2
 Identities = 4/7 (57%), Positives = 6/7 (85%)
 Frame = +3 / -2

                  
Query: 42  ILPWLYY 62
           +LPWL +
Sbjct: 456 LLPWLVF 436



>gb|CO516929.1|CO516929 s13dSG58F0300027_468404 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 468

 Score = 25.8 bits (50), Expect = 2.4
 Identities = 9/30 (30%), Positives = 16/30 (53%)
 Frame = +3 / +3

                                         
Query: 9   ITGSTSAGLHQILPWLYYHNVIGVSHFFLL 98
           +TG  +  LH I+PW +   +  +  F +L
Sbjct: 147 LTGCRATHLHLIIPWAWRELIFILLEFLIL 236



>gb|CO515258.1|CO515258 s13dSG96G0300024_399381 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 494

 Score = 25.4 bits (49), Expect = 3.3
 Identities = 8/23 (34%), Positives = 14/23 (60%)
 Frame = +2 / -3

                                  
Query: 17  KHICWPTPDSSMVVLSQCHWCFT 85
           K   WP P+SS++  ++  W F+
Sbjct: 393 KEFIWPKPESSIIKETR*IWSFS 325



>gb|CO515099.1|CO515099 s13dSG40B0600057_399063 Glandular trichomes Medicago sativa cDNA,
          mRNA sequence
          Length = 328

 Score = 24.9 bits (48), Expect = 4.6
 Identities = 8/20 (40%), Positives = 13/20 (65%)
 Frame = +1 / +2

                              
Query: 28 LAYTRFFHGCIITMSLVFHI 87
          L YT  + GCI  +++ FH+
Sbjct: 23 LLYT*IWRGCIFYVAVCFHV 82



>gb|CO512234.1|CO512234 s13dSG03E1200087_113982 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 588

 Score = 24.0 bits (46), Expect = 8.7
 Identities = 8/24 (33%), Positives = 15/24 (62%)
 Frame = +2 / +3

                                   
Query: 47  SMVVLSQCHWCFTFLSIS*RRGCK 118
           S+   S+C+W F   +I+ R+ C+
Sbjct: 327 SIQSFSRCYWWFVESTITCRQACR 398



>gb|CO513025.1|CO513025 s13dSG09G0300020_121976 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 503

 Score = 24.0 bits (46), Expect = 8.7
 Identities = 11/35 (31%), Positives = 18/35 (51%)
 Frame = +1 / -3

                                              
Query: 49  HGCIITMSLVFHISFY*LKERLQSQLXPLFLNLFS 153
           HGC +++S+ FH+    +  +    L   FL L S
Sbjct: 318 HGCSLSVSMQFHMPHLTIS*QRYQCLPQAFLTLSS 214



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  May 2, 2006  2:40 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 2,094,162
Number of Sequences: 7669
Number of extensions: 18841
Number of successful extensions: 1646
Number of sequences better than 10.0: 9
length of database: 1,248,568
effective HSP length: 40
effective length of database: 941,808
effective search space used: 71577408
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)