TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= F5H
(1360 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 204 e-123
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 201 e-122
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 157 1e-059
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 85 9e-041
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 85 1e-040
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 84 2e-040
gb|CO517155.1|CO517155 s13dSG33C0300022_474394 Glandular tr... 157 2e-039
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 65 1e-028
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 60 7e-025
gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular tr... 106 7e-024
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 84 4e-017
gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular tr... 56 1e-008
emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2 49 2e-006
gb|CO513530.1|CO513530 s13dSG12E0600039_139842 Glandular tr... 40 1e-005
gb|CB886623.1|CB886623 RX56 Medicago sativa cDNA-AFLP Medic... 41 4e-004
gb|CO512450.1|CO512450 s13dSG100B010000_114414 Glandular tr... 37 0.004
gb|CO512256.1|CO512256 s13dSG03G1200088_114026 Glandular tr... 33 0.078
gb|CO514039.1|CO514039 s13dSG72F0100011_156740 Glandular tr... 30 0.53
gb|CO517186.1|CO517186 s13dSG33F0800075_474456 Glandular tr... 26 0.79
gb|CO514748.1|CO514748 s13dSG55F1200103_328156 Glandular tr... 29 1.00
gb|CO515026.1|CO515026 s13dSG26E0700055_397557 Glandular tr... 28 2.6
gb|CO512408.1|CO512408 s13dSG80E1000071_114330 Glandular tr... 27 4.9
gb|M80235.1|ALFGRERP Alfalfa glucose-regulated endoplasmic ... 27 4.9
gb|CO513349.1|CO513349 s13dSG37H1200096_129862 Glandular tr... 27 6.7
gb|CO517117.1|CO517117 s13dSG29F0700063_468780 Glandular tr... 27 6.7
gb|AJ410110.1|AJ410110 AJ410110 Medicago sativa library (Ka... 26 9.2
gb|CO514687.1|CO514687 s13dSG46H0500048_328034 Glandular tr... 26 9.2
gb|CO515607.1|CO515607 s13dSG47E0300023_418273 Glandular tr... 26 9.2
gb|CO517194.1|CO517194 s13dSG33G0700056_474472 Glandular tr... 26 9.2
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 204 bits (440), Expect(3) = e-123
Identities = 82/140 (58%), Positives = 105/140 (75%)
Frame = +2 / +2
Query: 491 LHNTLRLTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDR 670
LHN+++LT+DNIKAIIMDVMFGGTETVASAIEWAMAE+M SP+DL+++QQELA+VVGL R
Sbjct: 962 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSR 1141
Query: 671 NVNESDLDKLPFLKCVIKETXXXXXXXXXXXXETAGDCVVGGYSVPRGSRVMVNVWAIGR 850
V E D +KL +LKC +KET ETA + V GY +P+ +RVM+N WAIGR
Sbjct: 1142 QVEEPDFEKLTYLKCALKETLRLHPPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGR 1321
Query: 851 HRASWKDADAFRPSRFTPEG 910
W++ ++F+PSRF G
Sbjct: 1322 DANCWEEPESFKPSRFLKPG 1381
Score = 157 bits (337), Expect(3) = e-123
Identities = 62/81 (76%), Positives = 68/81 (83%)
Frame = +2 / +2
Query: 926 DFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGDVFG 1105
DFKG FEF+PFGSGRRSCPG LGLYAL+LAVA L H F W LPDGMKPSE+DM DVFG
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1567
Query: 1106 LTAPRATRLYAVPTPRLNCPL 1168
LTAPRA+RL A+PT R+ CPL
Sbjct: 1568 LTAPRASRLIAIPTKRVLCPL 1630
Score = 114 bits (243), Expect(3) = e-123
Identities = 47/84 (55%), Positives = 59/84 (70%)
Frame = +2 / +2
Query: 137 IFNLTKNVTFRAAFGTRDGEDQEEFIAILQEFSKLFGAFNVVDFLPWLSWMDLQGINXXX 316
+FNLTKN+ +RAAFG+ E Q+EFI ILQEFSKLFGAFN+ DF+P +D QG+N
Sbjct: 626 VFNLTKNIIYRAAFGSSSREGQDEFIGILQEFSKLFGAFNISDFVPCFGAIDPQGLNARL 805
Query: 317 XXXXXXXXXFIDKIIDEHVRRGKN 388
FIDKIIDEHV++ K+
Sbjct: 806 VKARKDLDSFIDKIIDEHVQKKKS 877
Score = 82.6 bits (174), Expect(4) = 1e-041
Identities = 37/73 (50%), Positives = 50/73 (68%)
Frame = -2 / -2
Query: 1167 KGQLSRGVGTA*SLVARGAVRPKTSPMSSSEGFMPSGSDQLKPCASWATASSSAYRPSAV 988
+G +R VG A S +A GAV P TS +S S GF+PSG+ Q+K + ATA SSAY+P+ +
Sbjct: 1629 RGHKTRLVGIAMSRLALGAVSPNTSLISISLGFIPSGNSQVKQ*SK*ATAKSSAYKPNCI 1450
Query: 987 PGHERRPEPKGRN 949
PG + RP+P G N
Sbjct: 1449 PGQDLRPDPNGIN 1411
Score = 77.1 bits (162), Expect(4) = 1e-041
Identities = 38/77 (49%), Positives = 43/77 (55%)
Frame = -2 / -2
Query: 372 TCSSMILSMNXXXXXXXXXXXRLMPCRSIQLSHGRKSTTLKAPNSFENSCRMAMNSSWSS 193
TCSSMILSM L P SI G KS LKAPN+ ENSC + +NSS S
Sbjct: 861 TCSSMILSMKLSKSLRAFTSLALSP*GSIAPKQGTKSEILKAPNNLENSCNIPINSSCPS 682
Query: 192 PSRVPKAARNVTFLVRL 142
PKAAR + FLV+L
Sbjct: 681 LELDPKAARYIMFLVKL 631
Score = 40.9 bits (83), Expect(4) = 1e-041
Identities = 25/67 (37%), Positives = 29/67 (43%)
Frame = -2 / -2
Query: 723 LMTHLRKGSLSRSDSFTFXXXXXXXXXXXXXXXXXSGLCXXXXXXXXXXXATVSVPPNIT 544
L H R S S+S S T SGL ATVSVPPNIT
Sbjct: 1194 LRAHFR*VSFSKSGSSTCRLRPTTSASSCWTFLRSSGLFINSAIAHSIADATVSVPPNIT 1015
Query: 543 SMMIALI 523
S+M+AL+
Sbjct: 1014 SIMMALM 994
Score = 31.3 bits (62), Expect(4) = 1e-041
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = -2 / -2
Query: 894 RDGRNASASFHEARWRPMAHTLTMTREPLGTE*PPTTQSPA 772
RDG S S + P+AH +TR G +*P T S A
Sbjct: 1365 RDGLKLSGSSQQFASLPIAHAFIITRACFGMK*PLTVASSA 1243
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 201 bits (434), Expect(3) = e-122
Identities = 81/140 (57%), Positives = 103/140 (73%)
Frame = +2 / +1
Query: 491 LHNTLRLTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDR 670
LHN+++LT+DNIKAIIMDVMFGGTETVASAIEW MAE+M SP+DL+++QQELA+VVGL R
Sbjct: 976 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSR 1155
Query: 671 NVNESDLDKLPFLKCVIKETXXXXXXXXXXXXETAGDCVVGGYSVPRGSRVMVNVWAIGR 850
V E D +KL +LKC +KET ETA + V GY +P+ +RVM+N WAIGR
Sbjct: 1156 QVEEPDFEKLTYLKCALKETLRLHPPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGR 1335
Query: 851 HRASWKDADAFRPSRFTPEG 910
W + ++F+PSRF G
Sbjct: 1336 DANCWDEPESFKPSRFLKPG 1395
Score = 157 bits (337), Expect(3) = e-122
Identities = 62/81 (76%), Positives = 68/81 (83%)
Frame = +2 / +1
Query: 926 DFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGDVFG 1105
DFKG FEF+PFGSGRRSCPG LGLYAL+LAVA L H F W LPDGMKPSE+DM DVFG
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1581
Query: 1106 LTAPRATRLYAVPTPRLNCPL 1168
LTAPRA+RL A+PT R+ CPL
Sbjct: 1582 LTAPRASRLIAIPTKRVLCPL 1644
Score = 113 bits (242), Expect(3) = e-122
Identities = 47/84 (55%), Positives = 58/84 (69%)
Frame = +2 / +1
Query: 137 IFNLTKNVTFRAAFGTRDGEDQEEFIAILQEFSKLFGAFNVVDFLPWLSWMDLQGINXXX 316
+FNLTKN+ +RAAFG+ E Q+EFI ILQEFSKLFGAFN+ DF+P +D QG+N
Sbjct: 640 VFNLTKNIIYRAAFGSSSREGQDEFIGILQEFSKLFGAFNISDFVPCFGAIDPQGLNARL 819
Query: 317 XXXXXXXXXFIDKIIDEHVRRGKN 388
FIDKIIDEHV + K+
Sbjct: 820 VKARKDLDSFIDKIIDEHVEKKKS 891
Score = 84.0 bits (177), Expect(4) = 5e-040
Identities = 38/73 (52%), Positives = 50/73 (68%)
Frame = -2 / -1
Query: 1167 KGQLSRGVGTA*SLVARGAVRPKTSPMSSSEGFMPSGSDQLKPCASWATASSSAYRPSAV 988
KG +R VG A S +A GAV P TS +S S GF+PSG+ Q+K + ATA SSAY+P+ +
Sbjct: 1643 KGHKTRLVGIAMSRLALGAVSPNTSLISISLGFIPSGNSQVKQ*SK*ATAKSSAYKPNCI 1464
Query: 987 PGHERRPEPKGRN 949
PG + RP+P G N
Sbjct: 1463 PGQDLRPDPNGIN 1425
Score = 77.1 bits (162), Expect(4) = 5e-040
Identities = 38/77 (49%), Positives = 43/77 (55%)
Frame = -2 / -1
Query: 372 TCSSMILSMNXXXXXXXXXXXRLMPCRSIQLSHGRKSTTLKAPNSFENSCRMAMNSSWSS 193
TCSSMILSM L P SI G KS LKAPN+ ENSC + +NSS S
Sbjct: 875 TCSSMILSMKLSKSLRAFTSLALSP*GSIAPKQGTKSEILKAPNNLENSCNIPINSSCPS 696
Query: 192 PSRVPKAARNVTFLVRL 142
PKAAR + FLV+L
Sbjct: 695 LELDPKAARYIMFLVKL 645
Score = 34.1 bits (68), Expect(4) = 5e-040
Identities = 16/31 (51%), Positives = 19/31 (61%)
Frame = -2 / -1
Query: 615 GLCXXXXXXXXXXXATVSVPPNITSMMIALI 523
GL ATVSVPPNITS+M+AL+
Sbjct: 1100 GLFINSAITHSIADATVSVPPNITSIMMALM 1008
Score = 31.3 bits (62), Expect(4) = 5e-040
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = -2 / -1
Query: 894 RDGRNASASFHEARWRPMAHTLTMTREPLGTE*PPTTQSPA 772
RDG S S + P+AH +TR G +*P T S A
Sbjct: 1379 RDGLKLSGSSQQFASLPIAHAFIITRACFGMK*PLTVASSA 1257
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 157 bits (337), Expect(2) = 1e-059
Identities = 62/81 (76%), Positives = 68/81 (83%)
Frame = +2 / +2
Query: 926 DFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGDVFG 1105
DFKG FEF+PFGSGRRSCPG LGLYAL+LAVA L H F W LPDGMKPSE+DM DVFG
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 427
Query: 1106 LTAPRATRLYAVPTPRLNCPL 1168
LTAPRA+RL A+PT R+ CPL
Sbjct: 428 LTAPRASRLIAIPTKRVLCPL 490
Score = 88.6 bits (187), Expect(2) = 1e-059
Identities = 35/80 (43%), Positives = 47/80 (58%)
Frame = +2 / +2
Query: 674 VNESDLDKLPFLKCVIKETXXXXXXXXXXXXETAGDCVVGGYSVPRGSRVMVNVWAIGRH 853
V E D +KL +LKC +KET ETA + V GY +P+ +RVM+N WAIGR
Sbjct: 5 VEEPDFEKLTYLKCALKETLRLHPPIPLLLHETAEEATVNGYFIPKQARVMINAWAIGRD 184
Query: 854 RASWKDADAFRPSRFTPEGE 913
W + ++F+PSRF GE
Sbjct: 185 ANCWDEPESFKPSRFLKPGE 244
Score = 84.0 bits (177), Expect(2) = 9e-021
Identities = 38/73 (52%), Positives = 50/73 (68%)
Frame = -2 / -1
Query: 1167 KGQLSRGVGTA*SLVARGAVRPKTSPMSSSEGFMPSGSDQLKPCASWATASSSAYRPSAV 988
KG +R VG A S +A GAV P TS +S S GF+PSG+ Q+K + ATA SSAY+P+ +
Sbjct: 489 KGHKTRLVGIAMSRLALGAVSPNTSLISISLGFIPSGNSQVKQ*SK*ATAKSSAYKPNCI 310
Query: 987 PGHERRPEPKGRN 949
PG + RP+P G N
Sbjct: 309 PGQDLRPDPNGIN 271
Score = 31.3 bits (62), Expect(2) = 9e-021
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = -2 / -1
Query: 894 RDGRNASASFHEARWRPMAHTLTMTREPLGTE*PPTTQSPA 772
RDG S S + P+AH +TR G +*P T S A
Sbjct: 225 RDGLKLSGSSQQFASLPIAHAFIITRACFGMK*PLTVASSA 103
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 85.4 bits (180), Expect(4) = 9e-041
Identities = 30/76 (39%), Positives = 55/76 (72%)
Frame = +2 / +3
Query: 503 LRLTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNE 682
+++T+D+IK +++D GT++ A A EWA+AE++++P L + ++E+ VVG DR V+E
Sbjct: 819 IKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDE 998
Query: 683 SDLDKLPFLKCVIKET 730
D LP+++ ++KET
Sbjct: 999 VDTQNLPYIRAIVKET 1046
Score = 54.2 bits (112), Expect(4) = 9e-041
Identities = 23/48 (47%), Positives = 28/48 (58%)
Frame = +2 / +3
Query: 905 EGEAAGLDFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFN 1048
EGEA LD +G F+ LPFGSGRR CPG L + +A L F+
Sbjct: 1230 EGEARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFD 1373
Score = 52.8 bits (109), Expect(4) = 9e-041
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +2 / +3
Query: 779 DCVVGGYSVPRGSRVMVNVWAIGRHRASWKDADAFRPSRFTPEG 910
+C + GY +P G+ ++ NVW +GR W FRP RF G
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETG 1226
Score = 25.8 bits (50), Expect(4) = 9e-041
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2 / +3
Query: 1076 SELDMGDVFGLTAPRATRLYAVPTPRL 1156
+++ M + GLT PRA L VP R+
Sbjct: 1419 AKVSMEERAGLTVPRAHSLVCVPLARI 1499
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 85.4 bits (180), Expect(4) = 1e-040
Identities = 30/76 (39%), Positives = 55/76 (72%)
Frame = +2 / +3
Query: 503 LRLTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNE 682
+++T+D+IK +++D GT++ A A EWA+AE++++P L + ++E+ VVG DR V+E
Sbjct: 819 IKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVVGKDRLVDE 998
Query: 683 SDLDKLPFLKCVIKET 730
D LP+++ ++KET
Sbjct: 999 VDTQNLPYIRAIVKET 1046
Score = 54.2 bits (112), Expect(4) = 1e-040
Identities = 23/48 (47%), Positives = 28/48 (58%)
Frame = +2 / +3
Query: 905 EGEAAGLDFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFN 1048
EGEA LD +G F+ LPFGSGRR CPG L + +A L F+
Sbjct: 1230 EGEARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFD 1373
Score = 52.4 bits (108), Expect(4) = 1e-040
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = +2 / +3
Query: 779 DCVVGGYSVPRGSRVMVNVWAIGRHRASWKDADAFRPSRFTPEG 910
+C + GY +P G+ ++ NVW +GR W FRP RF G
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETG 1226
Score = 25.8 bits (50), Expect(4) = 1e-040
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2 / +3
Query: 1076 SELDMGDVFGLTAPRATRLYAVPTPRL 1156
+++ M + GLT PRA L VP R+
Sbjct: 1419 AKVSMEERAGLTVPRAHSLVCVPLARI 1499
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 84.0 bits (177), Expect(4) = 2e-040
Identities = 29/76 (38%), Positives = 56/76 (73%)
Frame = +2 / +3
Query: 503 LRLTRDNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNE 682
+++T++ IK +++D+ GT++ A A EWA+AE++++P L++ ++E+ VVG DR V+E
Sbjct: 819 IKITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVVGKDRLVDE 998
Query: 683 SDLDKLPFLKCVIKET 730
D LP+++ ++KET
Sbjct: 999 VDTQNLPYIRAIVKET 1046
Score = 54.7 bits (113), Expect(4) = 2e-040
Identities = 23/48 (47%), Positives = 28/48 (58%)
Frame = +2 / +3
Query: 905 EGEAAGLDFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFN 1048
EGEA LD +G F+ LPFGSGRR CPG L + +A L F+
Sbjct: 1230 EGEAGPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFD 1373
Score = 52.8 bits (109), Expect(4) = 2e-040
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +2 / +3
Query: 779 DCVVGGYSVPRGSRVMVNVWAIGRHRASWKDADAFRPSRFTPEG 910
+C + GY +P G+ V+ NVW +GR W FRP RF G
Sbjct: 1095 ECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETG 1226
Score = 25.8 bits (50), Expect(4) = 2e-040
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +2 / +3
Query: 1076 SELDMGDVFGLTAPRATRLYAVPTPRL 1156
+++ M + GLT PRA L VP R+
Sbjct: 1419 AKVSMEERAGLTVPRAHSLVCVPLARI 1499
>gb|CO517155.1|CO517155 s13dSG33C0300022_474394 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 571
Score = 157 bits (338), Expect = 2e-039
Identities = 63/81 (77%), Positives = 68/81 (83%)
Frame = +2 / -1
Query: 926 DFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGDVFG 1105
DFKG FEF+PFGSGRRSCPG LGLYAL+LAVA L H F W LPDGMKPSE+DM DVFG
Sbjct: 550 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 371
Query: 1106 LTAPRATRLYAVPTPRLNCPL 1168
LTAPRA+RL AVPT R+ CPL
Sbjct: 370 LTAPRASRLIAVPTKRVLCPL 308
Score = 85.4 bits (180), Expect = 1e-017
Identities = 38/73 (52%), Positives = 51/73 (69%)
Frame = -2 / +3
Query: 1167 KGQLSRGVGTA*SLVARGAVRPKTSPMSSSEGFMPSGSDQLKPCASWATASSSAYRPSAV 988
+G +R VGTA S +A GAV P TS +S S GF+PSG+ Q+K + ATA SSAY+P+ +
Sbjct: 309 RGHKTRLVGTAMSRLALGAVSPNTSLISISLGFIPSGNSQVKQ*SK*ATAKSSAYKPNCI 488
Query: 987 PGHERRPEPKGRN 949
PG + RP+P G N
Sbjct: 489 PGQDLRPDPNGIN 527
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 65.2 bits (136), Expect(3) = 1e-028
Identities = 27/71 (38%), Positives = 44/71 (61%)
Frame = +2 / +3
Query: 518 DNIKAIIMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNESDLDK 697
DN+ I+ ++ ET +IEW +AE+++ D ++++E+ V+G V E DL K
Sbjct: 912 DNVLYIVENINVAAIETTLWSIEWGIAELVNHQDIQNKVREEMDRVLGPGHQVTEPDLHK 1091
Query: 698 LPFLKCVIKET 730
LP+L+ VIKET
Sbjct: 1092 LPYLQAVIKET 1124
Score = 47.3 bits (97), Expect(3) = 1e-028
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +2 / +3
Query: 911 EAAGLDFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDG 1066
E + ++ G F +LPFG GRRSCPG L L L + + +L P G
Sbjct: 1302 EESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPG 1457
Score = 46.9 bits (96), Expect(3) = 1e-028
Identities = 16/43 (37%), Positives = 25/43 (58%)
Frame = +2 / +3
Query: 779 DCVVGGYSVPRGSRVMVNVWAIGRHRASWKDADAFRPSRFTPE 907
D + G+ +P S+++VN W + + A WK + FRP RF E
Sbjct: 1176 DPKLNGFDIPAESKILVNAWWLPNNPAHWKKPEEFRPERFLEE 1304
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 60.2 bits (125), Expect(3) = 7e-025
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +2 / +2
Query: 767 ETAGDCVVGGYSVPRGSRVMVNVWAIGRHRASWKDADAFRPSRF 898
E+ C + GY +P +RV+VN WAIGR W DA++F+P RF
Sbjct: 317 ESIKPCQINGYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPERF 448
Score = 57.4 bits (119), Expect(3) = 7e-025
Identities = 25/65 (38%), Positives = 41/65 (63%)
Frame = +2 / +2
Query: 536 IMDVMFGGTETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNESDLDKLPFLKC 715
+ D+ GGTE+ + + WAM+EM+ +P + + Q E+ V V+E++L +L +LK
Sbjct: 83 LQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRRVFDKKGYVDETELHQLIYLKS 262
Query: 716 VIKET 730
VIKET
Sbjct: 263 VIKET 277
Score = 29.0 bits (57), Expect(3) = 7e-025
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +2 / +2
Query: 926 DFKGGCFEFLPFGS 967
DFKG FE++PFGS
Sbjct: 464 DFKGTDFEYIPFGS 505
Score = 27.2 bits (53), Expect = 4.9
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -1 / -2
Query: 745 RVQPERLLDDALEEGELVQVRLVHV 671
R+QP DD L +LVQ+ L+H+
Sbjct: 292 RMQPHCFFDDGL*VYQLVQLCLIHI 218
>gb|CO513716.1|CO513716 s13dSG15C0700050_140214 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 300
Score = 106 bits (226), Expect = 7e-024
Identities = 41/98 (41%), Positives = 58/98 (59%)
Frame = +2 / +2
Query: 803 VPRGSRVMVNVWAIGRHRASWKDADAFRPSRFTPEGEAAGLDFKGGCFEFLPFGSGRRSC 982
+P+GS ++VNVWAI R W D F P RF P GE +D KG FE +PFG+GRR C
Sbjct: 5 IPKGSTLLVNVWAIARDPGKWADPLMFNPERFLPGGENCDVDVKGNDFEVIPFGAGRRIC 184
Query: 983 PGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGD 1096
G L + ++L +A L H N L +G+ +++M +
Sbjct: 185 AGLNLRIRMVQLQIATLVHSLNRELENGLNAKDINMDE 298
Score = 40.5 bits (82), Expect = 5e-004
Identities = 21/61 (34%), Positives = 22/61 (36%)
Frame = -2 / -1
Query: 987 PGHERRPEPKGRNXXXXXXXXXXXXXXSGVKRDGRNASASFHEARWRPMAHTLTMTREPL 808
P H RP PKG G G N + S H R MAHT T EP
Sbjct: 189 PAHILRPAPKGITSKSFPFTSTSQFSPPGKNLSGLNINGSAHFPGSRAMAHTFTKRVEPF 10
Query: 807 G 805
G
Sbjct: 9 G 7
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 84.0 bits (177), Expect = 4e-017
Identities = 33/70 (47%), Positives = 43/70 (61%)
Frame = +2 / +1
Query: 923 LDFKGGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDMGDVF 1102
+++KG FE +PFG+GRR CPG L + L VA L H F W+L DG+ P L+M + F
Sbjct: 73 INYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASLLHNFEWNLADGLIPEHLNMDEQF 252
Query: 1103 GLTAPRATRL 1132
GLT R L
Sbjct: 253 GLTLKRVQPL 282
Score = 28.6 bits (56), Expect = 1.9
Identities = 17/52 (32%), Positives = 18/52 (34%)
Frame = -2 / -1
Query: 1110 VRPKTSPMSSSEGFMPSGSDQLKPCASWATASSSAYRPSAVPGHERRPEPKG 955
V P S M G PS K C AT + PG P PKG
Sbjct: 260 VNPNCSSMFRCSGISPSARFHSKLCKRDATIKCIVL*ANGNPGQILLPAPKG 105
>gb|CO514181.1|CO514181 s13dSG70C1200086_157024 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 358
Score = 56.1 bits (116), Expect = 1e-008
Identities = 24/56 (42%), Positives = 36/56 (64%)
Frame = +2 / +1
Query: 563 ETVASAIEWAMAEMMHSPDDLRRLQQELADVVGLDRNVNESDLDKLPFLKCVIKET 730
ET AIEW +AE+++ D ++++E+ V+G V E DL KLP+L+ IKET
Sbjct: 127 ETTLWAIEWGIAELVNHQDIQNKVREEMDRVLGPGHQVTEPDLHKLPYLQAGIKET 294
>emb|Z50800.1|MSASET2MR M.sativa mRNA for ASET2
Length = 1193
Score = 48.7 bits (100), Expect = 2e-006
Identities = 18/18 (100%), Positives = 18/18 (100%)
Frame = -3 / -1
Query: 65 LVPNSCSPGDPLVLERPP 12
LVPNSCSPGDPLVLERPP
Sbjct: 65 LVPNSCSPGDPLVLERPP 12
Score = 39.1 bits (79), Expect = 0.001
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3 / +3
Query: 15 RPL*N*WIPRAAGIRHE 65
R L*N*WIPRAAGIRHE
Sbjct: 15 RTL*N*WIPRAAGIRHE 65
Score = 36.8 bits (74), Expect = 0.006
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -1 / -2
Query: 64 SCRIPAARGIH*F*SGR 14
SCRIPAARGIH*F*S R
Sbjct: 64 SCRIPAARGIH*F*SVR 14
Score = 34.5 bits (69), Expect = 0.030
Identities = 13/14 (92%), Positives = 13/14 (92%)
Frame = +2 / +2
Query: 14 AAALELVDPPGCRN 55
A ALELVDPPGCRN
Sbjct: 14 ADALELVDPPGCRN 55
>gb|CO513530.1|CO513530 s13dSG12E0600039_139842 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 499
Score = 40.0 bits (81), Expect(2) = 1e-005
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +2 / +3
Query: 935 GGCFEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNWSLP 1060
G F LPFGSGRR CPG L + ++ + F+ +P
Sbjct: 6 GQHFTLLPFGSGRRMCPGVNLATAGMATLLSSIIQCFDLQVP 131
Score = 24.0 bits (46), Expect(2) = 1e-005
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +2 / +3
Query: 1103 GLTAPRATRLYAVPTPR 1153
GLT PRA L VP R
Sbjct: 192 GLTVPRAHNLMCVPLAR 242
>gb|CB886623.1|CB886623 RX56 Medicago sativa cDNA-AFLP Medicago sativa cDNA, mRNA sequence
Length = 102
Score = 40.9 bits (83), Expect = 4e-004
Identities = 15/32 (46%), Positives = 19/32 (59%)
Frame = +2 / +3
Query: 1013 ELAVAQLAHGFNWSLPDGMKPSELDMGDVFGL 1108
E +AQL H FNW LPDGM E M + + +
Sbjct: 6 EFVLAQLVHCFNWDLPDGMSLDEFGMANWYAV 101
Score = 26.3 bits (51), Expect = 9.2
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = -2 / -3
Query: 1089 MSSSEGFMPSGSDQLKPCASWATASS 1012
M +S +PSG QLK C S A +S
Sbjct: 82 MPNSSRDIPSGRSQLKQCTS*ARTNS 5
>gb|CO512450.1|CO512450 s13dSG100B010000_114414 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 359
Score = 37.3 bits (75), Expect = 0.004
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = +2 / +1
Query: 983 PGTALGLYALELAVAQLAHGFNWSLPDGMKPSELDM 1090
PG+ L + L L + L + F+W L +GM+P ++++
Sbjct: 4 PGSPLAVRMLHLMLGSLINSFDWKLENGMEPKDMNL 111
>gb|CO512256.1|CO512256 s13dSG03G1200088_114026 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 615
Score = 33.1 bits (66), Expect = 0.078
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2 / +2
Query: 944 FEFLPFGSGRRSCPGTALGLYALELAVAQLAHGFNW 1051
+ F+PFG G R CPG + + + L F W
Sbjct: 74 YTFVPFGGGPRMCPGKEYARLEILVFMHNLVKRFKW 181
>gb|CO514039.1|CO514039 s13dSG72F0100011_156740 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 519
Score = 30.4 bits (60), Expect = 0.53
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2 / -3
Query: 1145 TPRLNCPLY*RHARATAITIVPSGGCGV 1228
TPRL+ P R +T +VP GGC V
Sbjct: 340 TPRLSSPTSSRGVVSTLPQLVPGGGCSV 257
>gb|CO517186.1|CO517186 s13dSG33F0800075_474456 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 647
Score = 26.3 bits (51), Expect(2) = 0.79
Identities = 8/16 (50%), Positives = 10/16 (62%)
Frame = +3 / -1
Query: 780 TASWAATPCPGAPASW 827
T +W + P PGA SW
Sbjct: 356 TKTWNSNPLPGASKSW 309
Score = 23.5 bits (45), Expect(2) = 0.79
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +2 / -1
Query: 269 LPWLSWMDLQGIN 307
LPWL W L G N
Sbjct: 494 LPWLRWEPLGGSN 456
>gb|CO514748.1|CO514748 s13dSG55F1200103_328156 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 380
Score = 29.5 bits (58), Expect = 1.00
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1 / +3
Query: 292 PAGHQPPPPRRTIRAGPVHRQD 357
P + PPPP I AGP+ R++
Sbjct: 165 PHRNPPPPPENDIEAGPLSRRN 230
>gb|CO515026.1|CO515026 s13dSG26E0700055_397557 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 456
Score = 28.1 bits (55), Expect = 2.6
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +1 / +2
Query: 292 PAGHQPPPPRRTIRAGPVHRQD 357
P + PPPP I AGP+ R++
Sbjct: 173 PHRNPPPPPVNDIEAGPLSRRN 238
>gb|CO512408.1|CO512408 s13dSG80E1000071_114330 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 357
Score = 27.2 bits (53), Expect = 4.9
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -1 / +1
Query: 1219 PTRGDDGNGSRPRMASVQGAVEPGRRHGVEPRGAR 1115
P R + RP+ + + E GRR G EPR AR
Sbjct: 133 PRRSNCSLEKRPQRRTGPKSPEKGRRKG*EPRCAR 237
>gb|M80235.1|ALFGRERP Alfalfa glucose-regulated endoplasmic reticular protein mRNA,
complete cds
Length = 1478
Score = 27.2 bits (53), Expect = 4.9
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = -1 / -3
Query: 1228 YPTPTRGDDGNGSRPRMASVQGAVEPGR 1145
Y PT+ D SRP S+ G ++ G+
Sbjct: 1401 YHKPTKETDCKSSRPSQKSISGKLQRGK 1318
>gb|CO513349.1|CO513349 s13dSG37H1200096_129862 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 581
Score = 26.7 bits (52), Expect = 6.7
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +1 / -2
Query: 241 VRRLQRRRLPAVAELDGPAGHQPPPPR 321
VR L R + + E GP PPPPR
Sbjct: 367 VRSLPRASILSFPETLGPLPPPPPPPR 287
>gb|CO517117.1|CO517117 s13dSG29F0700063_468780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 492
Score = 26.7 bits (52), Expect = 6.7
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = -2 / -3
Query: 1095 SPMSSSEGFMPSGSDQLKPCASWATASSSAYRP 997
S S+SEG + + PC W S SA P
Sbjct: 460 SKNSTSEGSTDQNRNGVSPCPRWTCRSQSA*LP 362
>gb|AJ410110.1|AJ410110 AJ410110 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U281, mRNA sequence
Length = 754
Score = 26.3 bits (51), Expect = 9.2
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3 / -3
Query: 65 LVPNSCSPG 39
LVPNSCSPG
Sbjct: 56 LVPNSCSPG 30
>gb|CO514687.1|CO514687 s13dSG46H0500048_328034 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 26.3 bits (51), Expect = 9.2
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2 / +2
Query: 209 FIAILQEFSKLFGAFNVVDFLPWLS 283
F A++ + LFG F VV F PW S
Sbjct: 410 FSAVILLY*GLFGWFCVVAF*PWFS 484
>gb|CO515607.1|CO515607 s13dSG47E0300023_418273 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 573
Score = 26.3 bits (51), Expect = 9.2
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = -2 / -1
Query: 1110 VRPKTSPMSSSEGFMPSGSDQLKPCASWATA 1018
+RP+T+P ++ P+G K SW T+
Sbjct: 453 LRPRTTPTTNDVSNSPTGQSISKGEPSWVTS 361
>gb|CO517194.1|CO517194 s13dSG33G0700056_474472 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 641
Score = 26.3 bits (51), Expect = 9.2
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -3 / +2
Query: 1358 FFFFFLRIKKIDNGCLQHTLQHRP 1287
FFFF+L +K + +T++H P
Sbjct: 80 FFFFYLSLKIFTHQNKNYTIRHTP 151
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,225,813
Number of Sequences: 7669
Number of extensions: 58094
Number of successful extensions: 1021
Number of sequences better than 10.0: 31
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 371741856
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)