TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8612115.2.1
(1078 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF530579.1| Medicago sativa high molecular weight root v... 72 6e-063
gb|CO516195.1|CO516195 s13dSG67E0800067_445718 Glandular tr... 154 3e-049
gb|CO511735.1|CO511735 s13dSG01E0300023_103370 Glandular tr... 73 1e-024
>gb|AF530579.1| Medicago sativa high molecular weight root vegetative storage
protein precursor, mRNA, partial cds
Length = 1319
Score = 71.6 bits (150), Expect(6) = 6e-063
Identities = 28/37 (75%), Positives = 29/37 (78%)
Frame = +2 / +3
Query: 521 SAAPQCPFPDASLGTALATGLFDYVWVQFYNNPPCQY 631
SAAPQC PD L A+ TGLFDYV VQFYNNPPCQY
Sbjct: 516 SAAPQCFMPDYYLDKAIKTGLFDYVLVQFYNNPPCQY 626
Score = 71.2 bits (149), Expect(6) = 6e-063
Identities = 30/49 (61%), Positives = 37/49 (75%)
Frame = +2 / +3
Query: 200 CTGVGADVKACQRMGVKVLLSIGGGVGSYGLSSRADARSVAAYLWDNYL 346
CT + ++K CQ+ GVKVLLSIGG VGSY LSS DA++VA YL N+L
Sbjct: 231 CTKLEPEIKHCQQKGVKVLLSIGGAVGSYSLSSPEDAKNVADYLHTNFL 377
Score = 53.3 bits (110), Expect(6) = 6e-063
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = +2 / +3
Query: 71 GXVXQTCATGNYRFVNVAFLPTFGKGQTPALNLAGHC 181
G + TC TGNY V +AFL FG G+ P N AGHC
Sbjct: 105 GTLTSTCDTGNYEIVLLAFLNVFGGGRVPNWNFAGHC 215
Score = 52.8 bits (109), Expect(6) = 6e-063
Identities = 17/37 (45%), Positives = 27/37 (72%)
Frame = +2 / +3
Query: 731 AAGSGFVPASDLVAQVLPVVKNSTKYGGIMLWSRYYD 841
A G++P DL+++V P +K ++ YGGIMLW R++D
Sbjct: 738 APSGGYIPPDDLISKVPPSIKPTSNYGGIMLWDRFHD 848
Score = 51.9 bits (107), Expect(6) = 6e-063
Identities = 20/28 (71%), Positives = 21/28 (75%)
Frame = +3 / +3
Query: 390 LGDAVLDGVDFDIESGGGMYWDDLARFL 473
LG LDGVDFDIE G +YWDDLAR L
Sbjct: 396 LGSVTLDGVDFDIEGGTNLYWDDLARDL 479
Score = 27.6 bits (54), Expect(6) = 6e-063
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +3 / +3
Query: 6 PGSPHSASRRXIAIYWGQNGNEG 74
P + ++S IAIYWGQN +G
Sbjct: 39 PFTIKASSSGGIAIYWGQNLGDG 107
>gb|CO516195.1|CO516195 s13dSG67E0800067_445718 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 154 bits (330), Expect(3) = 3e-049
Identities = 58/98 (59%), Positives = 70/98 (71%)
Frame = +2 / +1
Query: 53 GPERQRGXVXQTCATGNYRFVNVAFLPTFGKGQTPALNLAGHCDPASGGCTGVGADVKAC 232
G G + C T NY+FVN+AFL TFG GQTP LNLAGHCDPAS GCT ++++ C
Sbjct: 139 GQNGNEGSLADACNTNNYQFVNIAFLSTFGNGQTPTLNLAGHCDPASNGCTKFSSEIQTC 318
Query: 233 QRMGVKVLLSIGGGVGSYGLSSRADARSVAAYLWDNYL 346
Q G++VLLS+GGG GSY LSS DA VA YLW+N+L
Sbjct: 319 QSKGIRVLLSLGGGAGSYSLSSADDATQVANYLWNNFL 432
Score = 79.0 bits (166), Expect(3) = 2e-021
Identities = 43/90 (47%), Positives = 47/90 (52%)
Frame = -2 / -1
Query: 339 LSQRYAATLRASARDESP*LPTPPPMESRTLTPMRWHALTSAPTPVHPPLAGSQWPARLS 160
L R AT AS+ +E *LP PPP +S TL P+ S V P AGSQWPAR
Sbjct: 425 LFHR*FATCVASSAEERE*LPAPPPRDSNTLMPLL*QV*ISLLNFVQPFEAGSQWPARFR 246
Query: 159 AGVCPLPNVGRKATLTNL*XPVAQVCXTSP 70
GV PLP V RKA TN * V Q P
Sbjct: 245 VGVWPLPKVDRKAMFTN**LFVLQASAKEP 156
Score = 40.0 bits (81), Expect(3) = 3e-049
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +3 / +1
Query: 390 LGDAVLDGVDFDIESGGGMYWD 455
L DAVLDG+DFDIE+GG Y D
Sbjct: 457 LXDAVLDGIDFDIEAGGEHYDD 522
Score = 35.0 bits (70), Expect(3) = 3e-049
Identities = 12/18 (66%), Positives = 14/18 (77%)
Frame = +3 / +1
Query: 21 SASRRXIAIYWGQNGNEG 74
S+ IA+YWGQNGNEG
Sbjct: 106 SSHAAGIAVYWGQNGNEG 159
Score = 28.6 bits (56), Expect(3) = 2e-021
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = -1 / -1
Query: 439 PPLSMSKSTPSRTAS 395
PP S+SKS PS+TAS
Sbjct: 506 PPASISKSIPSKTAS 462
Score = 26.7 bits (52), Expect(3) = 2e-021
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = -1 / -1
Query: 73 PSLPFWPQ*MAM 38
PS PFWPQ* A+
Sbjct: 158 PSFPFWPQ*TAI 123
>gb|CO511735.1|CO511735 s13dSG01E0300023_103370 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 411
Score = 72.5 bits (152), Expect(2) = 1e-024
Identities = 27/51 (52%), Positives = 37/51 (72%)
Frame = +2 / +2
Query: 731 AAGSGFVPASDLVAQVLPVVKNSTKYGGIMLWSRYYDGLTGYSDAVKSYV* 883
A GF+P+ L +QVLP +K S KYGG+M+W R+ DG +GYS+A+K V*
Sbjct: 161 APSGGFIPSDVLNSQVLPAIKGSAKYGGVMIWDRFNDGQSGYSNAIKGSV* 313
Score = 55.6 bits (115), Expect(2) = 1e-024
Identities = 19/21 (90%), Positives = 20/21 (95%)
Frame = +2 / +2
Query: 575 TGLFDYVWVQFYNNPPCQYSA 637
TGLFDYVWVQFYNNP CQYS+
Sbjct: 5 TGLFDYVWVQFYNNPQCQYSS 67
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,478,811
Number of Sequences: 7669
Number of extensions: 77162
Number of successful extensions: 811
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 289422315
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)