TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 6293482.2.1
(2042 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 88 2e-054
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 88 1e-053
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 63 1e-039
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 61 3e-039
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 61 9e-039
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 70 2e-031
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 89 2e-028
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 82 1e-023
gb|CO516404.1|CO516404 s13dSG27C1100084_446136 Glandular tr... 99 2e-021
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 88.1 bits (186), Expect(6) = 2e-054
Identities = 31/76 (40%), Positives = 51/76 (67%)
Frame = -2 / +2
Query: 511 GGVDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQLPAGMKAEDVDMTE 332
G D K + F+F+ FGSGRR CPG+ ++ +++L++ F W+LP GMK ++DM++
Sbjct: 1379 GVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSD 1558
Query: 331 VFGITVSRKEKLLLVP 284
VFG+T R +L+ +P
Sbjct: 1559 VFGLTAPRASRLIAIP 1606
Score = 61.6 bits (128), Expect(6) = 2e-054
Identities = 27/82 (32%), Positives = 43/82 (52%)
Frame = -2 / +2
Query: 1573 LRLGAVPTVVVSSPRVAEAVLRTYDHVFSSRPRSLVSDIIMYGATDSCFAPYGDHFRKAR 1394
LR+G + V +S A VL+ D++FS+RP ++ + Y D FA YG +R+ R
Sbjct: 317 LRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGPFWRQMR 496
Query: 1393 KLVTVHLLNASKVRSQRPAREE 1328
KL + L + S + R+E
Sbjct: 497 KLCVMKLFSRKHAESWQSVRDE 562
Score = 56.5 bits (117), Expect(6) = 2e-054
Identities = 24/66 (36%), Positives = 41/66 (62%)
Frame = -2 / +2
Query: 943 LQKEYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEVRRNVPAGQ 764
L LT+D+IKAI++D+ GT+T +E+AM EL++ P +KK+Q+E+ V +
Sbjct: 962 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVVGLSR 1141
Query: 763 EMVTED 746
++ D
Sbjct: 1142 QVEEPD 1159
Score = 55.1 bits (114), Expect(6) = 2e-054
Identities = 21/42 (50%), Positives = 25/42 (59%)
Frame = -2 / +2
Query: 640 VAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFLSGDV 515
V GY IP RV++NAWA+GR + WE F P RFL V
Sbjct: 1259 VNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLKPGV 1384
Score = 32.2 bits (64), Expect(6) = 2e-054
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2 / +2
Query: 1072 AKTRRVRKRWDDLLDKLIDDHAARMATHQDEDDD 971
A+ + RK D +DK+ID+H + + DE+ D
Sbjct: 797 ARLVKARKDLDSFIDKIIDEHVQKKKSVVDEETD 898
Score = 24.4 bits (47), Expect(6) = 2e-054
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = -2 / +2
Query: 1660 IIGHLHLMGDIPYVSLAALATRYG 1589
+IG++ +M + + LA LA +YG
Sbjct: 233 VIGNMLMMDQLTHRGLANLAKKYG 304
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 88.1 bits (186), Expect(6) = 1e-053
Identities = 31/76 (40%), Positives = 51/76 (67%)
Frame = -2 / +1
Query: 511 GGVDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQLPAGMKAEDVDMTE 332
G D K + F+F+ FGSGRR CPG+ ++ +++L++ F W+LP GMK ++DM++
Sbjct: 1393 GVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSD 1572
Query: 331 VFGITVSRKEKLLLVP 284
VFG+T R +L+ +P
Sbjct: 1573 VFGLTAPRASRLIAIP 1620
Score = 61.6 bits (128), Expect(6) = 1e-053
Identities = 27/82 (32%), Positives = 43/82 (52%)
Frame = -2 / +1
Query: 1573 LRLGAVPTVVVSSPRVAEAVLRTYDHVFSSRPRSLVSDIIMYGATDSCFAPYGDHFRKAR 1394
LR+G + V +S A VL+ D++FS+RP ++ + Y D FA YG +R+ R
Sbjct: 331 LRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGPFWRQMR 510
Query: 1393 KLVTVHLLNASKVRSQRPAREE 1328
KL + L + S + R+E
Sbjct: 511 KLCVMKLFSRKHAESWQSVRDE 576
Score = 54.7 bits (113), Expect(6) = 1e-053
Identities = 23/66 (34%), Positives = 40/66 (60%)
Frame = -2 / +1
Query: 943 LQKEYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEVRRNVPAGQ 764
L LT+D+IKAI++D+ GT+T +E+ M EL++ P +KK+Q+E+ V +
Sbjct: 976 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVVGLSR 1155
Query: 763 EMVTED 746
++ D
Sbjct: 1156 QVEEPD 1173
Score = 53.8 bits (111), Expect(6) = 1e-053
Identities = 20/42 (47%), Positives = 25/42 (59%)
Frame = -2 / +1
Query: 640 VAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFLSGDV 515
V GY IP RV++NAWA+GR + W+ F P RFL V
Sbjct: 1273 VNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPGV 1398
Score = 32.2 bits (64), Expect(6) = 1e-053
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -2 / +1
Query: 1072 AKTRRVRKRWDDLLDKLIDDHAARMATHQDEDDD 971
A+ + RK D +DK+ID+H + + DE+ D
Sbjct: 811 ARLVKARKDLDSFIDKIIDEHVEKKKSVVDEETD 912
Score = 24.9 bits (48), Expect(6) = 1e-053
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -2 / +1
Query: 1660 IIGHLHLMGDIPYVSLAALATRYG 1589
IIG++ +M + + LA LA +YG
Sbjct: 247 IIGNMLMMDQLTHRGLANLAKKYG 318
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 62.9 bits (131), Expect(4) = 1e-039
Identities = 22/48 (45%), Positives = 29/48 (60%)
Frame = -2 / +3
Query: 652 DACEVAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFLSGDVAG 509
+ CE+ GY IP ++ N W +GR S YW+R +EF PERFL G
Sbjct: 1092 EECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETGAEG 1235
Score = 61.1 bits (127), Expect(4) = 1e-039
Identities = 22/49 (44%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 934 EYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEV 788
E +T+DHIK +++D F AGTD++ + E+A+ ELI P +++K +EEV
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEV 962
Score = 56.1 bits (116), Expect(4) = 1e-039
Identities = 22/45 (48%), Positives = 33/45 (73%)
Frame = -2 / +3
Query: 505 VDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQL 371
+DL+ FQ L FGSGRRMCPGV+ A++ + +L++L+ FD Q+
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 46.4 bits (95), Expect(4) = 1e-039
Identities = 20/73 (27%), Positives = 38/73 (52%)
Frame = -2 / +3
Query: 1426 APYGDHFRKARKLVTVHLLNASKVRSQRPAREEEVXXXXXXXXXXXXXREPVDMSELLHS 1247
AP+G +++ RKL+ LLNA+ V RP R +++ ++P+D++E L
Sbjct: 324 APFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQIRKFLRVMAQGAEAQKPLDLTEELLK 503
Query: 1246 FVNNLVCRAVSGK 1208
+ N+ + G+
Sbjct: 504 WTNSTTSMMMLGE 542
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 61.1 bits (127), Expect(4) = 3e-039
Identities = 22/48 (45%), Positives = 28/48 (58%)
Frame = -2 / +3
Query: 652 DACEVAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFLSGDVAG 509
+ CE+ GY IP V+ N W +GR YW+R +EF PERFL G
Sbjct: 1092 EECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETGAEG 1235
Score = 59.7 bits (124), Expect(4) = 3e-039
Identities = 24/48 (50%), Positives = 35/48 (72%)
Frame = -2 / +3
Query: 514 AGGVDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQL 371
AG +DL+ FQ L FGSGRRMCPGV+ A++ + +L++L+ FD Q+
Sbjct: 1239 AGPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 57.0 bits (118), Expect(4) = 3e-039
Identities = 20/49 (40%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 934 EYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEV 788
E +T++ IK +++D+F AGTD++ + E+A+ ELI P +++K +EEV
Sbjct: 816 EIKITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEV 962
Score = 47.3 bits (97), Expect(4) = 3e-039
Identities = 20/72 (27%), Positives = 38/72 (52%)
Frame = -2 / +3
Query: 1423 PYGDHFRKARKLVTVHLLNASKVRSQRPAREEEVXXXXXXXXXXXXXREPVDMSELLHSF 1244
P+G ++R RKL+ LLNA+ V RP R +++ ++P+D++E L +
Sbjct: 327 PFGPYWRFVRKLIMNDLLNATTVNKLRPLRTQQIRKFLRVMAQSAEAQKPLDVTEELLKW 506
Query: 1243 VNNLVCRAVSGK 1208
N+ + + G+
Sbjct: 507 TNSTISMMMLGE 542
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 61.1 bits (127), Expect(4) = 9e-039
Identities = 22/49 (44%), Positives = 36/49 (73%)
Frame = -2 / +3
Query: 934 EYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEV 788
E +T+DHIK +++D F AGTD++ + E+A+ ELI P +++K +EEV
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEV 962
Score = 60.6 bits (126), Expect(4) = 9e-039
Identities = 21/48 (43%), Positives = 28/48 (58%)
Frame = -2 / +3
Query: 652 DACEVAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFLSGDVAG 509
+ CE+ GY IP ++ N W +GR YW+R +EF PERFL G
Sbjct: 1092 EECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETGAEG 1235
Score = 56.1 bits (116), Expect(4) = 9e-039
Identities = 22/45 (48%), Positives = 33/45 (73%)
Frame = -2 / +3
Query: 505 VDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQL 371
+DL+ FQ L FGSGRRMCPGV+ A++ + +L++L+ FD Q+
Sbjct: 1248 LDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 46.0 bits (94), Expect(4) = 9e-039
Identities = 19/72 (26%), Positives = 38/72 (52%)
Frame = -2 / +3
Query: 1423 PYGDHFRKARKLVTVHLLNASKVRSQRPAREEEVXXXXXXXXXXXXXREPVDMSELLHSF 1244
P+G +++ RKL+ LLNA+ V RP R +++ ++P+D++E L +
Sbjct: 327 PFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQIRKLLRVMAQGAEAQKPLDLTEELLKW 506
Query: 1243 VNNLVCRAVSGK 1208
N+ + + G+
Sbjct: 507 TNSTISMMMLGE 542
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 70.3 bits (147), Expect(3) = 2e-031
Identities = 35/87 (40%), Positives = 48/87 (55%)
Frame = -2 / +3
Query: 1585 DLMLLRLGAVPTVVVSSPRVAEAVLRTYDHVFSSRPRSLVSDIIMYGATDSCFAPYGDHF 1406
++ LLR+G VVVSSP +A+ VL T F SR R++V DI D F YG+H+
Sbjct: 228 EIFLLRMGQRNLVVVSSPELAKEVLHTQCVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHW 407
Query: 1405 RKARKLVTVHLLNASKVRSQRPAREEE 1325
RK R+++TV V+ R E E
Sbjct: 408 RKMRRIMTVPFFTNKVVQQYRYGWESE 488
Score = 68.4 bits (143), Expect(3) = 2e-031
Identities = 32/77 (41%), Positives = 47/77 (61%)
Frame = -2 / +3
Query: 757 VTEDNLPGMTDLKAVIKETLRXXXXXXXXXXHYSLDACEVAGYTIPANTRVVVNAWALGR 578
VTE +L + L+AVIKETLR H +L ++ G+ IPA ++++VNAW L
Sbjct: 1068 VTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDPKLNGFDIPAESKILVNAWWLPN 1247
Query: 577 HSGYWERENEFVPERFL 527
+ +W++ EF PERFL
Sbjct: 1248 NPAHWKKPEEFRPERFL 1298
Score = 40.0 bits (81), Expect(3) = 2e-031
Identities = 17/53 (32%), Positives = 26/53 (49%)
Frame = -2 / +3
Query: 505 VDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQLPAGMKAED 347
V+ N+F++L FG GRR CPG+ A + + L+ + P G D
Sbjct: 1314 VEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPGQSKID 1472
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 88.6 bits (187), Expect(2) = 2e-028
Identities = 31/76 (40%), Positives = 51/76 (67%)
Frame = -2 / +2
Query: 511 GGVDLKPNEFQFLAFGSGRRMCPGVHSASATIEAMLSNLMYRFDWQLPAGMKAEDVDMTE 332
G D K + F+F+ FGSGRR CPG+ ++ +++L++ F W+LP GMK ++DM++
Sbjct: 239 GEPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSD 418
Query: 331 VFGITVSRKEKLLLVP 284
VFG+T R +L+ +P
Sbjct: 419 VFGLTAPRASRLIAIP 466
Score = 53.3 bits (110), Expect(2) = 2e-028
Identities = 19/38 (50%), Positives = 24/38 (63%)
Frame = -2 / +2
Query: 640 VAGYTIPANTRVVVNAWALGRHSGYWERENEFVPERFL 527
V GY IP RV++NAWA+GR + W+ F P RFL
Sbjct: 119 VNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFL 232
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 82.2 bits (173), Expect(2) = 1e-023
Identities = 36/78 (46%), Positives = 45/78 (57%)
Frame = -2 / +2
Query: 757 VTEDNLPGMTDLKAVIKETLRXXXXXXXXXXHYSLDACEVAGYTIPANTRVVVNAWALGR 578
V E L + LK+VIKET+R S+ C++ GY IPA TRV+VNAWA+GR
Sbjct: 221 VDETELHQLIYLKSVIKETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGR 400
Query: 577 HSGYWERENEFVPERFLS 524
YW F PERFL+
Sbjct: 401 DPRYWVDAESFKPERFLN 454
Score = 43.7 bits (89), Expect(2) = 1e-023
Identities = 18/39 (46%), Positives = 28/39 (71%)
Frame = -2 / +2
Query: 898 LIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEVRR 782
L DMF GT++S + +AM+E+++ P +M+K Q EVRR
Sbjct: 83 LQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVRR 199
>gb|CO516404.1|CO516404 s13dSG27C1100084_446136 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 98.7 bits (209), Expect = 2e-021
Identities = 42/87 (48%), Positives = 60/87 (68%)
Frame = -2 / +3
Query: 1582 LMLLRLGAVPTVVVSSPRVAEAVLRTYDHVFSSRPRSLVSDIIMYGATDSCFAPYGDHFR 1403
LM L+LG + ++VSS +A+ VL+T+D F +RP L+S I+ Y A D F+ YG+H+R
Sbjct: 246 LMHLKLGEISYIIVSSSEMAKEVLKTHDLTFCNRPNLLLSTILSYNARDIAFSTYGEHWR 425
Query: 1402 KARKLVTVHLLNASKVRSQRPAREEEV 1322
+ RK+ V LLNA +V+S R REEEV
Sbjct: 426 QLRKICVVELLNAKRVQSFRSIREEEV 506
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,220,779
Number of Sequences: 7669
Number of extensions: 133418
Number of successful extensions: 1748
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 573699005
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)