TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4695478.2.1
(1201 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 61 4e-032
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 58 3e-030
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 73 1e-027
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 60.6 bits (126), Expect(4) = 4e-032
Identities = 27/52 (51%), Positives = 34/52 (65%)
Frame = -2 / +1
Query: 1116 AMSTGRTEEAAGSAETACVTXAXXXIASWLVKLLLSRGYTVHGTVRDLSDNK 961
A+S R + ++ CVT A IASWLV+LLL+RGYTV TVRD +D K
Sbjct: 22 AVSKKREKLSSNLGNVVCVTGASGYIASWLVRLLLNRGYTVKATVRDPNDPK 177
Score = 56.1 bits (116), Expect(4) = 4e-032
Identities = 23/44 (52%), Positives = 29/44 (65%)
Frame = -2 / +1
Query: 963 KTAHLKRLENASENLKLFKADVLDYDAMAGAVAGCQGVFHVATP 832
K HL +LE A E L+LFKA++L+ A V GC GVFH A+P
Sbjct: 178 KGDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASP 309
Score = 42.3 bits (86), Expect(4) = 4e-032
Identities = 17/50 (34%), Positives = 26/50 (52%)
Frame = -2 / +1
Query: 690 NPKDWPKDKIKDENCWSDKEFCRNEENWYSVAKISSEEAALEYAKQTGLD 541
N K D + DE ++D + C WY V+K +EEAA ++ K +D
Sbjct: 451 NGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKFVKVNNID 600
Score = 29.5 bits (58), Expect(4) = 4e-032
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -2 / +1
Query: 816 ITDPEREMLGPAVTGTINAL 757
+ DP+ E++ PA+ GT+N L
Sbjct: 322 VKDPQAELIDPALKGTLNVL 381
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 58.3 bits (121), Expect(4) = 3e-030
Identities = 27/52 (51%), Positives = 33/52 (63%)
Frame = -2 / +1
Query: 1116 AMSTGRTEEAAGSAETACVTXAXXXIASWLVKLLLSRGYTVHGTVRDLSDNK 961
A+S R + ++ CVT A IASWLV LLL+RGYTV TVRD +D K
Sbjct: 22 AVSKKREKMSSNLGNVVCVTGASGYIASWLV*LLLNRGYTVKATVRDPNDPK 177
Score = 57.0 bits (118), Expect(4) = 3e-030
Identities = 23/44 (52%), Positives = 29/44 (65%)
Frame = -2 / +1
Query: 963 KTAHLKRLENASENLKLFKADVLDYDAMAGAVAGCQGVFHVATP 832
K HL +LE A E L+LFKA++L+ A V GC GVFH A+P
Sbjct: 178 KVDHLVKLEGAKERLQLFKANLLEEGAFDSVVQGCHGVFHTASP 309
Score = 37.3 bits (75), Expect(4) = 3e-030
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = -2 / +1
Query: 690 NPKDWPKDKIKDENCWSDKEFCRNEENWYSVAKISSEEAALEY 562
N K D + DE ++D + C WY V+K +EEAA ++
Sbjct: 451 NGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAWKF 579
Score = 29.5 bits (58), Expect(4) = 3e-030
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -2 / +1
Query: 816 ITDPEREMLGPAVTGTINAL 757
+ DP+ E++ PA+ GT+N L
Sbjct: 322 VKDPQAELIDPALKGTLNVL 381
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 73.5 bits (154), Expect(3) = 1e-027
Identities = 33/110 (30%), Positives = 60/110 (54%)
Frame = -2 / +2
Query: 606 YSVAKISSEEAALEYAKQTGLDVVTVNPAVVFGPLLQPTLNTSCQFLVYFLKGGPDRMRD 427
Y+V+K +E+A LE+ +Q G+DVVT+ + G + P L S + + + G +++
Sbjct: 542 YAVSKTLAEKAVLEFGEQNGIDVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIGV 721
Query: 426 KLWHIVDVRDTADALLLVYETPQASGRHICAPHFISARDLLELLKTMYPD 277
+H+V V D A A + + E GR+ C+P + ++ +LL YP+
Sbjct: 722 TRFHMVHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYPE 871
Score = 50.1 bits (103), Expect(3) = 1e-027
Identities = 19/41 (46%), Positives = 26/41 (63%)
Frame = -2 / +2
Query: 951 LKRLENASENLKLFKADVLDYDAMAGAVAGCQGVFHVATPV 829
L L ASE L F AD+ + D+ A A+ GC G+FH A+P+
Sbjct: 197 LTNLPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPI 319
Score = 39.1 bits (79), Expect(3) = 1e-027
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = -2 / +2
Query: 1110 STGRTEEAAGSAETACVTXAXXXIASWLVKLLLSRGYTVHGTVR 979
S ++ + A CVT + SW++K LL GY+V+ T+R
Sbjct: 32 SQKKSVQMAEGKGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIR 163
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,673,412
Number of Sequences: 7669
Number of extensions: 124267
Number of successful extensions: 1180
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 327093156
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)