TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4679577.2.1
(833 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 102 8e-045
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 102 8e-045
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 102 2e-040
gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular tr... 138 4e-035
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 102 bits (217), Expect(3) = 8e-045
Identities = 34/60 (56%), Positives = 49/60 (81%)
Frame = +2 / +2
Query: 320 NTQWPCQAGKGYYGRGPLQISWNFNYGPAGQSIGFDGLGDPDAVARSAVLAFRSALWYWM 499
++++PC +GK YYGRGP+QISWN+NYG G++IG D L +PD VA V++F++ALW+WM
Sbjct: 539 SSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 718
Score = 59.7 bits (124), Expect(3) = 8e-045
Identities = 23/46 (50%), Positives = 28/46 (60%)
Frame = +2 / +2
Query: 122 CEANGFYTRDAFLAAAGYYPAFGRTGTVDDSKREIAAFFGNANHET 259
C+ G YT DAF++AA +P F G KREIAAF G +HET
Sbjct: 311 CQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 448
Score = 51.5 bits (106), Expect(3) = 8e-045
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = +2 / +2
Query: 533 GFGATIRAINGALECDGKNPNSVNNRVAYYKQFCQDFGVDPGNNLTC 673
G+G ING LEC V +R+ +YK++C GV G NL C
Sbjct: 809 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 949
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 102 bits (217), Expect(3) = 8e-045
Identities = 34/60 (56%), Positives = 49/60 (81%)
Frame = +2 / +1
Query: 320 NTQWPCQAGKGYYGRGPLQISWNFNYGPAGQSIGFDGLGDPDAVARSAVLAFRSALWYWM 499
++++PC +GK YYGRGP+QISWN+NYG G++IG D L +PD VA V++F++ALW+WM
Sbjct: 538 SSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 717
Score = 59.7 bits (124), Expect(3) = 8e-045
Identities = 23/46 (50%), Positives = 28/46 (60%)
Frame = +2 / +1
Query: 122 CEANGFYTRDAFLAAAGYYPAFGRTGTVDDSKREIAAFFGNANHET 259
C+ G YT DAF++AA +P F G KREIAAF G +HET
Sbjct: 310 CQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 447
Score = 51.5 bits (106), Expect(3) = 8e-045
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = +2 / +1
Query: 533 GFGATIRAINGALECDGKNPNSVNNRVAYYKQFCQDFGVDPGNNLTC 673
G+G ING LEC V +R+ +YK++C GV G NL C
Sbjct: 808 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 948
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 102 bits (217), Expect(3) = 2e-040
Identities = 34/60 (56%), Positives = 49/60 (81%)
Frame = +2 / +1
Query: 320 NTQWPCQAGKGYYGRGPLQISWNFNYGPAGQSIGFDGLGDPDAVARSAVLAFRSALWYWM 499
++++PC +GK YYGRGP+QISWN+NYG G++IG D L +PD VA V++F++ALW+WM
Sbjct: 508 SSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 687
Score = 51.5 bits (106), Expect(3) = 2e-040
Identities = 19/47 (40%), Positives = 25/47 (53%)
Frame = +2 / +1
Query: 533 GFGATIRAINGALECDGKNPNSVNNRVAYYKQFCQDFGVDPGNNLTC 673
G+G ING LEC V +R+ +YK++C GV G NL C
Sbjct: 778 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 918
Score = 44.6 bits (91), Expect(3) = 2e-040
Identities = 17/41 (41%), Positives = 22/41 (53%)
Frame = +2 / +1
Query: 122 CEANGFYTRDAFLAAAGYYPAFGRTGTVDDSKREIAAFFGN 244
C+ G YT DAF++AA +P F G KRE F G+
Sbjct: 277 CQGKGLYTYDAFISAAKAFPNFANNGDTATKKREDCCFLGS 399
>gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 138 bits (295), Expect(2) = 4e-035
Identities = 57/113 (50%), Positives = 69/113 (61%)
Frame = +2 / +2
Query: 20 QSGPCDVPETNNASVASIVTPXXXXXXXXXXXXSCEANGFYTRDAFLAAAGYYPAFGRTG 199
Q P +N+ +VA IVT SC FY+R AFL A Y FGR+G
Sbjct: 248 QISPSTPGPSNDVNVADIVTQEFFNSIIDQADSSCAGKNFYSRAAFLDALNSYNQFGRSG 427
Query: 200 TVDDSKREIAAFFGNANHETIKFCYINEIDGPSKNYCDRNNTQWPCQAGKGYY 358
+VDDSKREIAA F + HET+ FCYI EIDG SK+YCD +NT++PC KGYY
Sbjct: 428 SVDDSKREIAAAFAHFTHETLHFCYIEEIDGASKDYCDESNTKYPCAPNKGYY 586
Score = 24.9 bits (48), Expect(2) = 4e-035
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / +2
Query: 8 GAGCQSGPC 34
G GCQ GPC
Sbjct: 212 GTGCQQGPC 238
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,430,937
Number of Sequences: 7669
Number of extensions: 52763
Number of successful extensions: 627
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 217720450
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)