TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4574602.2.1
(1248 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF530579.1| Medicago sativa high molecular weight root v... 76 4e-067
gb|CO516195.1|CO516195 s13dSG67E0800067_445718 Glandular tr... 243 5e-065
gb|CO511735.1|CO511735 s13dSG01E0300023_103370 Glandular tr... 76 2e-030
>gb|AF530579.1| Medicago sativa high molecular weight root vegetative storage
protein precursor, mRNA, partial cds
Length = 1319
Score = 75.8 bits (159), Expect(6) = 4e-067
Identities = 32/51 (62%), Positives = 37/51 (72%)
Frame = -2 / +3
Query: 914 CTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGG 762
CT +I+ CQ G+KVLLSIGGA GSY LSS +DA +VADYL NFL G
Sbjct: 231 CTKLEPEIKHCQQKGVKVLLSIGGAVGSYSLSSPEDAKNVADYLHTNFLSG 383
Score = 75.3 bits (158), Expect(6) = 4e-067
Identities = 27/56 (48%), Positives = 37/56 (66%)
Frame = -2 / +3
Query: 1100 VGTTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHC 933
+ +S+G IA+YWGQN +G+L C++G Y V +AFL FG G+ P N AGHC
Sbjct: 48 IKASSSGGIAIYWGQNLGDGTLTSTCDTGNYEIVLLAFLNVFGGGRVPNWNFAGHC 215
Score = 60.2 bits (125), Expect(6) = 4e-067
Identities = 26/51 (50%), Positives = 31/51 (60%)
Frame = -2 / +3
Query: 677 DLANALKGKGSVLLTAAPQCPYPDASLGPALQTGQFDNVWIQFYNNPGCAY 525
DL N + L+AAPQC PD L A++TG FD V +QFYNNP C Y
Sbjct: 474 DLDNLRQQNRYFYLSAAPQCFMPDYYLDKAIKTGLFDYVLVQFYNNPPCQY 626
Score = 56.5 bits (117), Expect(6) = 4e-067
Identities = 22/41 (53%), Positives = 26/41 (63%)
Frame = -2 / +3
Query: 425 AAGSGYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFNDVQNN 303
A GYI P L V P+I+ NYGGIM+WDRF+DV N
Sbjct: 738 APSGGYIPPDDLISKVPPSIKPTSNYGGIMLWDRFHDVTGN 860
Score = 49.2 bits (101), Expect(6) = 4e-067
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = -2 / +3
Query: 761 SGSSRPLGAAVLDGIDFDIENGQSAHYDDLANAL 660
SG PLG+ LDG+DFDIE G + ++DDLA L
Sbjct: 378 SGQFGPLGSVTLDGVDFDIEGGTNLYWDDLARDL 479
Score = 26.3 bits (51), Expect(6) = 4e-067
Identities = 8/18 (44%), Positives = 11/18 (61%)
Frame = -2 / +3
Query: 521 NGDDTNLVNAWNTWTSSI 468
N D L+ +WN WTS +
Sbjct: 636 NSDPKLLLQSWNAWTSLV 689
>gb|CO516195.1|CO516195 s13dSG67E0800067_445718 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 530
Score = 243 bits (524), Expect = 5e-065
Identities = 99/139 (71%), Positives = 112/139 (80%)
Frame = -2 / +1
Query: 1094 TTSAGNIAVYWGQNGNEGSLADACNSGLYAYVNIAFLTTFGNGQTPVLNLAGHCDPGSGS 915
++ A IAVYWGQNGNEGSLADACN+ Y +VNIAFL+TFGNGQTP LNLAGHCDP S
Sbjct: 106 SSHAAGIAVYWGQNGNEGSLADACNTNNYQFVNIAFLSTFGNGQTPTLNLAGHCDPASNG 285
Query: 914 CTGQSSDIQTCQSLGIKVLLSIGGASGSYGLSSTDDANSVADYLWDNFLGGSGSSRPLGA 735
CT SS+IQTCQS GI+VLLS+GG +GSY LSS DDA VA+YLW+NFLGG+ SS PL
Sbjct: 286 CTKFSSEIQTCQSKGIRVLLSLGGGAGSYSLSSADDATQVANYLWNNFLGGTSSSGPLXD 465
Query: 734 AVLDGIDFDIENGQSAHYD 678
AVLDGIDFDIE G + D
Sbjct: 466 AVLDGIDFDIEAGGEHYDD 522
Score = 147 bits (316), Expect = 2e-036
Identities = 75/127 (59%), Positives = 88/127 (69%)
Frame = +1 / -1
Query: 697 PFSMSKSMPSSTAAPRGRDEPLPPRKLSHR*SATLLASSVEERP*LPLAPPMERSTLMPS 876
P S+SKS+PS TA+ G ++ +PP+KL HR* AT +ASS EER *LP PP + +TLMP
Sbjct: 503 PASISKSIPSKTASXNGPEDDVPPKKLFHR*FATCVASSAEERE*LPAPPPRDSNTLMPL 324
Query: 877 DWQVWMSLLWPVQLPEPGSQCPARLRTGVCPLPKVVRNAMLT*A*SPELQASARLPSLPF 1056
QV +SLL VQ E GSQ PAR R GV PLPKV R AM T * LQASA+ PS PF
Sbjct: 323 L*QV*ISLLNFVQPFEAGSQWPARFRVGVWPLPKVDRKAMFTN**LFVLQASAKEPSFPF 144
Query: 1057 WPQYTAM 1077
WPQ TA+
Sbjct: 143 WPQ*TAI 123
>gb|CO511735.1|CO511735 s13dSG01E0300023_103370 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 411
Score = 75.8 bits (159), Expect(2) = 2e-030
Identities = 26/37 (70%), Positives = 30/37 (81%)
Frame = -2 / +2
Query: 581 TGQFDNVWIQFYNNPGCAYANGDDTNLVNAWNTWTSS 471
TG FD VW+QFYNNP C Y++G+ NLVNAWN WTSS
Sbjct: 5 TGLFDYVWVQFYNNPQCQYSSGNTNNLVNAWNQWTSS 115
Score = 71.6 bits (150), Expect(2) = 2e-030
Identities = 27/51 (52%), Positives = 35/51 (68%)
Frame = -2 / +2
Query: 425 AAGSGYIDPGTLTGTVIPAIRGIGNYGGIMVWDRFNDVQNNYSSQVKGSV* 273
A G+I L V+PAI+G YGG+M+WDRFND Q+ YS+ +KGSV*
Sbjct: 161 APSGGFIPSDVLNSQVLPAIKGSAKYGGVMIWDRFNDGQSGYSNAIKGSV* 313
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,616,363
Number of Sequences: 7669
Number of extensions: 97240
Number of successful extensions: 674
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 341793972
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)