TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4424526.2.1
(1446 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase 279 4e-097
gb|U17436.1|MSU17436 Medicago sativa isoflavone reductase (... 99 4e-072
gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase... 234 2e-062
gb|CO514976.1|CO514976 s13dSG63G0800068_397457 Glandular tr... 226 7e-060
gb|CO511690.1|CO511690 s13dSG01A0300021_103280 Glandular tr... 220 3e-058
gb|CO512559.1|CO512559 s13dSG08E0900067_121044 Glandular tr... 85 6e-032
gb|CO515541.1|CO515541 s13dSG53C0200018_418141 Glandular tr... 82 4e-030
gb|CO517063.1|CO517063 s13dSG31H0600058_468672 Glandular tr... 102 2e-029
>emb|X58078.1|MSISREDMR M.sativa mRNA for isoflavone reductase
Length = 1181
Score = 279 bits (603), Expect(3) = 4e-097
Identities = 115/237 (48%), Positives = 156/237 (65%)
Frame = -1 / +1
Query: 1299 TAPSDPAKAALLKSFQDAGVTLLKGDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVD 1120
TA + K L+ ++Q GV LL+GD+ D +LV A+K D+VI G + I DQ +++
Sbjct: 196 TAANPETKEELIDNYQSLGVILLEGDINDHETLVKAIKQVDIVICAAGRLLIEDQVKIIK 375
Query: 1119 AIKEAGNVKRFFPSEFGLDVDRTGIVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAG 940
AIKEAGNVK+FFPSEFGLDVDR VEP + + K IRR EA G+PYTY F G
Sbjct: 376 AIKEAGNVKKFFPSEFGLDVDRHDAVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTG 555
Query: 939 FGLPKVGQVLAPGPPADKAVVLGDGDTKAVFVEEGDIATYTVLAADDPRAENKVLYIKPP 760
+ L + Q+ A PP DK V+LGDG+ K +V E D+ T+T+ AA+DP NK ++I+ P
Sbjct: 556 YFLRNLAQLDATDPPRDKVVILGDGNVKGAYVTEADVGTFTIRAANDPNTLNKAVHIRLP 735
Query: 759 ANTLSHNELLSLWEKKTGKTFRREYVPEEAVLKQIQESPIPLNIILAIGHAAFVRGE 589
N L+ NE+++LWEKK GKT + YV EE VLK IQES P N +LA+ H+ ++G+
Sbjct: 736 KNYLTQNEVIALWEKKIGKTLEKTYVSEEQVLKDIQESSFPHNYLLALYHSQQIKGD 906
Score = 91.8 bits (194), Expect(3) = 4e-029
Identities = 45/96 (46%), Positives = 59/96 (61%)
Frame = +2 / -1
Query: 968 V*GMPAASVARRMPTLAPRMDLAGSTMPVRSTSRPNSDGKNLLTFPASLMASTSRLWSAI 1147
V*G P+AS+ R+ + + L GST RSTS PNSDGKN LTFPASL+A S+I
Sbjct: 527 V*GTPSASITLRILAFSSKTCLTGSTAS*RSTSSPNSDGKNFLTFPASLIALIILT*SSI 348
Query: 1148 CIDPSTEMTTSAPFTALTRLAWSYRSPLRSVTPASW 1255
P+ ++T S ALTR++ S+ SP +TP W
Sbjct: 347 SSLPAAQITMSTCLIALTRVS*SFISPSSRITPKDW 240
Score = 61.1 bits (127), Expect(3) = 4e-097
Identities = 23/32 (71%), Positives = 28/32 (87%)
Frame = -1 / +1
Query: 579 FEIDPAKGVDASELYPDVKYTTVDEYLNRFL* 484
+EIDPAK ++ASE YPDV YTT DEYLN+F+*
Sbjct: 913 YEIDPAKDIEASEAYPDVTYTTADEYLNQFV* 1008
Score = 50.1 bits (103), Expect(3) = 4e-097
Identities = 20/35 (57%), Positives = 28/35 (80%)
Frame = -1 / +1
Query: 1401 KSKILVVGGTGYLGRHVVAASARLGHPTSALVRDT 1297
++KIL++G TG +GRH+V AS + G+PT ALVR T
Sbjct: 61 ENKILILGPTGAIGRHIVWASIKAGNPTYALVRKT 165
Score = 38.6 bits (78), Expect(3) = 4e-029
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = +2 / -1
Query: 500 RYSSTVVYLTSGYSSLASTPLAGSIS 577
+YSS VVY+TSG +S AS LAGSIS
Sbjct: 992 KYSSAVVYVTSG*ASEASISLAGSIS 915
Score = 37.7 bits (76), Expect(3) = 4e-029
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = +2 / -1
Query: 1307 TSAEVGWPSRALAATTCLPRYPVPPTTRIL 1396
T A VG+P+ LA T CLP PV P RIL
Sbjct: 155 TKAYVGFPALILAQTMCLPIAPVGPRMRIL 66
>gb|U17436.1|MSU17436 Medicago sativa isoflavone reductase (IFR) gene, complete cds
Length = 2730
Score = 99.1 bits (210), Expect(5) = 4e-072
Identities = 40/80 (50%), Positives = 50/80 (62%)
Frame = -1 / +3
Query: 1095 KRFFPSEFGLDVDRTGIVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQ 916
++FFPSEFGLDVDR VEP + + K IRR EA G+PYTY F G+ L + Q
Sbjct: 1428 QKFFPSEFGLDVDRHEAVEPVRQVFEEKASIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQ 1607
Query: 915 VLAPGPPADKAVVLGDGDTK 856
+ PP DK V+LGDG+ K
Sbjct: 1608 LDTTDPPRDKVVILGDGNVK 1667
Score = 88.6 bits (187), Expect(5) = 4e-072
Identities = 34/66 (51%), Positives = 47/66 (71%)
Frame = -1 / +2
Query: 849 FVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELLSLWEKKTGKTFRREYVPEEA 670
+V E D+ T+T+ AA+DP NK ++I+ P N L+ NE+++LWEKK GKT + YV EE
Sbjct: 1769 YVTEADVGTFTIRAANDPNTLNKAVHIRLPENYLTQNEVIALWEKKIGKTLEKTYVSEEQ 1948
Query: 669 VLKQIQ 652
VLK IQ
Sbjct: 1949 VLKDIQ 1966
Score = 61.1 bits (127), Expect(5) = 4e-072
Identities = 23/32 (71%), Positives = 28/32 (87%)
Frame = -1 / +3
Query: 579 FEIDPAKGVDASELYPDVKYTTVDEYLNRFL* 484
+EIDPAK ++ASE YPDV YTT DEYLN+F+*
Sbjct: 2268 YEIDPAKDIEASEAYPDVTYTTADEYLNQFV* 2363
Score = 58.8 bits (122), Expect(5) = 4e-072
Identities = 24/47 (51%), Positives = 33/47 (70%)
Frame = -1 / +2
Query: 1233 LKGDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVK 1093
L+GD+ D +LV A+K D+VI G + I DQ +++ AIKEAGNVK
Sbjct: 1151 LQGDINDHETLVKAIKQVDIVICAAGRLLIEDQVKIIKAIKEAGNVK 1291
Score = 50.1 bits (103), Expect(5) = 4e-072
Identities = 20/35 (57%), Positives = 28/35 (80%)
Frame = -1 / +3
Query: 1401 KSKILVVGGTGYLGRHVVAASARLGHPTSALVRDT 1297
++KIL++G TG +GRH+V AS + G+PT ALVR T
Sbjct: 867 ENKILILGPTGAIGRHIVWASIKAGNPTYALVRKT 971
Score = 47.8 bits (98), Expect(4) = 4e-021
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +2 / -3
Query: 968 V*GMPAASVARRMPTLAPRMDLAGSTMPVRSTSRPNSDGKN 1090
V*G P+AS+ R+ + + L GST RSTS PNSDGKN
Sbjct: 1555 V*GTPSASITLRILAFSSKTCLTGSTAS*RSTSSPNSDGKN 1433
Score = 39.1 bits (79), Expect(4) = 4e-021
Identities = 21/47 (44%), Positives = 29/47 (61%)
Frame = +2 / -1
Query: 1094 LTFPASLMASTSRLWSAICIDPSTEMTTSAPFTALTRLAWSYRSPLR 1234
LTFPASL+A S+I P+ ++T S ALTR++ S+ SP R
Sbjct: 1290 LTFPASLIALIILT*SSISSLPAAQITMSTCLIALTRVS*SFISPCR 1150
Score = 38.6 bits (78), Expect(4) = 4e-021
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = +2 / -3
Query: 500 RYSSTVVYLTSGYSSLASTPLAGSIS 577
+YSS VVY+TSG +S AS LAGSIS
Sbjct: 2347 KYSSAVVYVTSG*ASEASISLAGSIS 2270
Score = 37.7 bits (76), Expect(4) = 4e-021
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = +2 / -3
Query: 1307 TSAEVGWPSRALAATTCLPRYPVPPTTRIL 1396
T A VG+P+ LA T CLP PV P RIL
Sbjct: 961 TKAYVGFPALILAQTMCLPIAPVGPRIRIL 872
>gb|AF201458.1|AF201458 Medicago sativa isoflavone reductase-like NAD(P)H-dependent
oxidoreductase (IRL1) mRNA, complete cds
Length = 1220
Score = 234 bits (505), Expect = 2e-062
Identities = 103/182 (56%), Positives = 127/182 (69%)
Frame = -1 / +2
Query: 1407 SEKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLK 1228
+EKSKIL +GGTGY+G+H+V ASA+ GHPT ALVR++ +DPAKA LL +F+ GV L+
Sbjct: 26 AEKSKILFIGGTGYIGKHIVEASAKAGHPTFALVRESTLADPAKANLLNNFKTLGVNLVP 205
Query: 1227 GDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTG 1048
GDLYD +LV +K DVVIS +G I DQ +++ AIKEAGNVKRFFPS FG DVDR
Sbjct: 206 GDLYDHQNLVKVIKQVDVVISTVGHALIEDQVKIIAAIKEAGNVKRFFPSAFGNDVDRVH 385
Query: 1047 IVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPPADKAVVLGD 868
V+PAKS K IRRA EA GIPYTY + +FAG+ LP + Q PP K V+
Sbjct: 386 AVDPAKSAFYGKAKIRRAIEAEGIPYTYVSSNYFAGYFLPTLAQPGQFAPPPPKDKVVIY 565
Query: 867 GD 862
GD
Sbjct: 566 GD 571
Score = 199 bits (428), Expect = 1e-051
Identities = 79/143 (55%), Positives = 108/143 (75%)
Frame = -1 / +2
Query: 909 APGPPADKAVVLGDGDTKAVFVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELL 730
AP PP DK V+ GDG+ KAVF +E DI T+T+ A D P NK+LYIKPP + S NEL+
Sbjct: 530 APPPPKDKVVIYGDGNPKAVFNKEDDIGTFTIRAVDAPGPWNKILYIKPPKESYSSNELV 709
Query: 729 SLWEKKTGKTFRREYVPEEAVLKQIQESPIPLNIILAIGHAAFVRGEQTGFEIDPAKGVD 550
+LWEKK+GK ++ +PE+ +LK IQE+PIP+ ++L I H FV+G+ T F I+P+ GV+
Sbjct: 710 ALWEKKSGKPAQKPSLPEDNLLKDIQEAPIPITVVLPIHHGVFVKGDHTNFVIEPSFGVE 889
Query: 549 ASELYPDVKYTTVDEYLNRFL*A 481
A ELYPDVKYTTV+EYL++ +*+
Sbjct: 890 AYELYPDVKYTTVEEYLDQSV*S 958
Score = 141 bits (303), Expect(2) = 2e-047
Identities = 79/196 (40%), Positives = 101/196 (51%)
Frame = +2 / -3
Query: 818 VYVAMSPSSTNTALVSPSPRTTALSAGGPGARTCPTLGRXXXXXXXXXXXV*GMPAASVA 997
V ++ S +T L SP T + GG +GR V*GMP+AS+A
Sbjct: 615 VPMSSSLLNTALGLPSPYMTTLSFGGGGANCPG*ANVGRKYPAK*LLETYV*GMPSASIA 436
Query: 998 RRMPTLAPRMDLAGSTMPVRSTSRPNSDGKNLLTFPASLMASTSRLWSAICIDPSTEMTT 1177
RR+ D AGST RSTS PN++GKNLLT PASL+A+ S+I P+ +MTT
Sbjct: 435 RRILAFP*NADFAGSTAWTRSTSFPNAEGKNLLTLPASLIAAIIFT*SSIRAWPTVDMTT 256
Query: 1178 SAPFTALTRLAWSYRSPLRSVTPASWKXXXXXXXXXXXXXXXXTSAEVGWPSRALAATTC 1357
S T+ SYRSP +TP K T+A+VG P+ ALA+T C
Sbjct: 255 STCLITFTKF**SYRSPGTKLTPKVLKLLRRLALAGSARVDSRTNAKVGCPAFALASTIC 76
Query: 1358 LPRYPVPPTTRILLFS 1405
P PVPP +ILLFS
Sbjct: 75 FPM*PVPPMNKILLFS 28
Score = 67.5 bits (141), Expect(2) = 2e-047
Identities = 37/61 (60%), Positives = 39/61 (63%)
Frame = +2 / -3
Query: 500 RYSSTVVYLTSGYSSLASTPLAGSISKPVCSPRTNAACPIARMMFRGIGDSWICLSTASS 679
RYSSTVVYLTSGY+S ASTP GSI+K V SP TN I GIG S I L SS
Sbjct: 939 RYSSTVVYLTSGYNS*ASTPKDGSITKFV*SPFTNTPWWIGNTTVIGIGAS*ISLRRLSS 760
Query: 680 G 682
G
Sbjct: 759 G 757
>gb|CO514976.1|CO514976 s13dSG63G0800068_397457 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 480
Score = 226 bits (487), Expect = 7e-060
Identities = 95/157 (60%), Positives = 117/157 (74%)
Frame = -1 / +1
Query: 1407 SEKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLK 1228
+EKSKIL +GGTGY+G+H+V ASA+ GHPT ALVR++ +DPAKA LL +F+ GV L+
Sbjct: 7 AEKSKILFIGGTGYIGKHIVEASAKAGHPTFALVRESTLADPAKANLLNNFKTLGVNLVP 186
Query: 1227 GDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTG 1048
GDLYD +LV +K DVVIS +G I DQ +++ AIKEAGNVKRFFPSEFG DVDR
Sbjct: 187 GDLYDHENLVKVIKQVDVVISTVGHALIEDQVKIIAAIKEAGNVKRFFPSEFGNDVDRVH 366
Query: 1047 IVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGF 937
V+PAKS K IRRA EA GIPYTY + +FAG+
Sbjct: 367 AVDPAKSAFEGKAKIRRAIEAEGIPYTYVSSNYFAGY 477
Score = 138 bits (296), Expect = 2e-033
Identities = 69/146 (47%), Positives = 86/146 (58%)
Frame = +2 / -2
Query: 968 V*GMPAASVARRMPTLAPRMDLAGSTMPVRSTSRPNSDGKNLLTFPASLMASTSRLWSAI 1147
V*GMP+AS+ARR+ D AGST RSTS PNS+GKNLLT PASL+A+ S+I
Sbjct: 446 V*GMPSASIARRILAFPSNADFAGSTAWTRSTSFPNSEGKNLLTLPASLIAAIIFT*SSI 267
Query: 1148 CIDPSTEMTTSAPFTALTRLAWSYRSPLRSVTPASWKXXXXXXXXXXXXXXXXTSAEVGW 1327
P+ +MTTS T+ + SYRSP +TP K T+A+VG
Sbjct: 266 RAWPTVDMTTSTCLITFTKFS*SYRSPGTKLTPKVLKLLRRLALAGSARVDSRTNAKVGC 87
Query: 1328 PSRALAATTCLPRYPVPPTTRILLFS 1405
P+ ALA+T C P PVPP +ILLFS
Sbjct: 86 PAFALASTICFPM*PVPPMNKILLFS 9
>gb|CO511690.1|CO511690 s13dSG01A0300021_103280 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 220 bits (475), Expect = 3e-058
Identities = 95/159 (59%), Positives = 115/159 (72%)
Frame = -1 / +1
Query: 1407 SEKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLK 1228
+EKSKIL +GGTGY+G+H+V ASA+ GHPT ALVR++ +DPAKA LL +F+ GV L+
Sbjct: 91 AEKSKILFIGGTGYIGKHIVEASAKAGHPTFALVRESTLADPAKANLLNNFKTLGVNLVP 270
Query: 1227 GDLYDQASLVSAVKGADVVISVLGSMQIADQSRLVDAIKEAGNVKRFFPSEFGLDVDRTG 1048
GDLYD +LV +K DVVIS +G I DQ +++ AIKEAGNVKRFFPSEFG DVDR
Sbjct: 271 GDLYDHENLVKVIKQVDVVISTVGHALIEDQVKIIAAIKEAGNVKRFFPSEFGNDVDRVH 450
Query: 1047 IVEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGL 931
V+PAKS K IRRA EA GIPYTY AG+ L
Sbjct: 451 AVDPAKSAFEGKAKIRRAIEAEGIPYTYVSQQLLAGYFL 567
Score = 138 bits (296), Expect = 2e-033
Identities = 69/146 (47%), Positives = 86/146 (58%)
Frame = +2 / -2
Query: 968 V*GMPAASVARRMPTLAPRMDLAGSTMPVRSTSRPNSDGKNLLTFPASLMASTSRLWSAI 1147
V*GMP+AS+ARR+ D AGST RSTS PNS+GKNLLT PASL+A+ S+I
Sbjct: 530 V*GMPSASIARRILAFPSNADFAGSTAWTRSTSFPNSEGKNLLTLPASLIAAIIFT*SSI 351
Query: 1148 CIDPSTEMTTSAPFTALTRLAWSYRSPLRSVTPASWKXXXXXXXXXXXXXXXXTSAEVGW 1327
P+ +MTTS T+ + SYRSP +TP K T+A+VG
Sbjct: 350 RAWPTVDMTTSTCLITFTKFS*SYRSPGTKLTPKVLKLLRRLALAGSARVDSRTNAKVGC 171
Query: 1328 PSRALAATTCLPRYPVPPTTRILLFS 1405
P+ ALA+T C P PVPP +ILLFS
Sbjct: 170 PAFALASTICFPM*PVPPMNKILLFS 93
>gb|CO512559.1|CO512559 s13dSG08E0900067_121044 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 573
Score = 85.4 bits (180), Expect(3) = 6e-032
Identities = 40/94 (42%), Positives = 53/94 (56%)
Frame = -1 / +1
Query: 1404 EKSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKG 1225
EKSK+LVVGGTGY+GR +V AS GH T L R D K +L SF+ G L++G
Sbjct: 55 EKSKVLVVGGTGYIGRRIVKASLEQGHETYVLQRPDIGLDTEKMQMLLSFKKLGAHLVEG 234
Query: 1224 DLYDQASLVSAVKGADVVISVLGSMQIADQSRLV 1123
+ SLV AVK DVVI + + + ++
Sbjct: 235 SFSNHQSLVDAVKLVDVVICTMSGVHFRSHNLML 336
Score = 45.5 bits (93), Expect(3) = 6e-032
Identities = 17/25 (68%), Positives = 22/25 (88%)
Frame = -1 / +1
Query: 1137 QSRLVDAIKEAGNVKRFFPSEFGLD 1063
Q +L++AIK+A NVKRF PSEFG+D
Sbjct: 337 QLKLIEAIKDAENVKRFLPSEFGMD 411
Score = 40.0 bits (81), Expect(3) = 6e-032
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = -1 / +1
Query: 1044 VEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQV 913
+EP + K+ IR+A E A IP+TY A FAG+ + Q+
Sbjct: 433 LEPGRVTFDDKMAIRKAIEDANIPFTYISANCFAGYFAGNLSQM 564
>gb|CO515541.1|CO515541 s13dSG53C0200018_418141 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 539
Score = 81.7 bits (172), Expect(3) = 4e-030
Identities = 38/93 (40%), Positives = 52/93 (55%)
Frame = -1 / +2
Query: 1401 KSKILVVGGTGYLGRHVVAASARLGHPTSALVRDTAPSDPAKAALLKSFQDAGVTLLKGD 1222
KSK+LVVGGTGY+GR +V AS GH T L R + K +L SF+ G L++G
Sbjct: 62 KSKVLVVGGTGYIGRRIVKASLEQGHETYVLQRPDIGLETEKVQMLLSFKKLGAHLVEGS 241
Query: 1221 LYDQASLVSAVKGADVVISVLGSMQIADQSRLV 1123
+ SLV AVK DVVI + + + ++
Sbjct: 242 FSNHQSLVDAVKLVDVVICTMSGVHFRSHNLML 340
Score = 49.6 bits (102), Expect(3) = 4e-030
Identities = 19/25 (76%), Positives = 23/25 (92%)
Frame = -1 / +2
Query: 1137 QSRLVDAIKEAGNVKRFFPSEFGLD 1063
Q +LV+AIK+AGNVKRF PSEFG+D
Sbjct: 341 QLKLVEAIKDAGNVKRFLPSEFGMD 415
Score = 33.1 bits (66), Expect(3) = 4e-030
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -1 / +2
Query: 1044 VEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFA 943
+EP + K+ IR+ E A IP+TY A FA
Sbjct: 437 LEPGRVTFDEKMTIRKTIEDANIPFTYISANCFA 538
>gb|CO517063.1|CO517063 s13dSG31H0600058_468672 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 102 bits (218), Expect(2) = 2e-029
Identities = 41/107 (38%), Positives = 62/107 (57%)
Frame = -1 / +1
Query: 891 DKAVVLGDGDTKAVFVEEGDIATYTVLAADDPRAENKVLYIKPPANTLSHNELLSLWEKK 712
DK V+ GDG+ K V+++E D+ATYT+ DDPR NK +YI+PP N L+ EL+ WEK
Sbjct: 166 DKVVLYGDGNVKVVYMDEDDVATYTIKTIDDPRTLNKTIYIRPPENILTQRELVEKWEKL 345
Query: 711 TGKTFRREYVPEEAVLKQIQESPIPLNIILAIGHAAFVRGEQTGFEI 571
TGK + + E+ L ++ + + + + F G T FE+
Sbjct: 346 TGKQLEKSTISEQDFLSSMKGLDLAGQVAVGHFYHIFFEGCLTNFEL 486
Score = 41.8 bits (85), Expect(2) = 2e-029
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = -1 / +2
Query: 1044 VEPAKSILGAKVGIRRATEAAGIPYTYAVAGFFAGFGLPKVGQVLAPGPP 895
+EP + K+ IR+A E A IP+TY A FAG+ + Q+ PP
Sbjct: 14 LEPGRVTFDDKMAIRKAIEDANIPFTYISANCFAGYFAGNLSQMGTLFPP 163
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,512,184
Number of Sequences: 7669
Number of extensions: 78361
Number of successful extensions: 631
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 398164684
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)