TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4424417.2.1
(1137 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol deh... 213 9e-057
gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehyd... 213 9e-057
gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol deh... 150 3e-037
emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol d... 150 3e-037
gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehyd... 150 3e-037
>gb|AF083333.1|AF083333 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad1) mRNA,
complete cds
Length = 1390
Score = 213 bits (459), Expect(2) = 9e-057
Identities = 88/147 (59%), Positives = 115/147 (78%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSP K+ EA+D+LGAD+F+VSKD E+MKAA TMD II+T+SA +L P +GLLK NG
Sbjct: 735 ISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNG 914
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++ VGLP+KPLE+ F L+ G K + GS IGGM++TQEM++ KH +TADIEL+
Sbjct: 915 KLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHE 1094
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLKN 562
+NTAMERL KADV+YRFVID+ N+ +
Sbjct: 1095 INTAMERLHKADVKYRFVIDVANSFSS 1175
Score = 22.6 bits (43), Expect(2) = 9e-057
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +2 / +3
Query: 2 SPMKYHGLNVP 34
SPMKY+G+ P
Sbjct: 615 SPMKYYGMTEP 647
>gb|L46856.1|ALFCAD1A Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, complete
cds
Length = 1390
Score = 213 bits (459), Expect(2) = 9e-057
Identities = 88/147 (59%), Positives = 115/147 (78%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
ISTSP K+ EA+D+LGAD+F+VSKD E+MKAA TMD II+T+SA +L P +GLLK NG
Sbjct: 735 ISTSPNKETEAIDKLGADSFLVSKDPEKMKAAMGTMDYIIDTISAAHSLMPLLGLLKLNG 914
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K++ VGLP+KPLE+ F L+ G K + GS IGGM++TQEM++ KH +TADIEL+
Sbjct: 915 KLVTVGLPSKPLELSVFPLVAGRKLIGGSNIGGMKETQEMLDFCGKHNITADIELIKMHE 1094
Query: 482 VNTAMERLAKADVRYRFVIDIGNTLKN 562
+NTAMERL KADV+YRFVID+ N+ +
Sbjct: 1095 INTAMERLHKADVKYRFVIDVANSFSS 1175
Score = 22.6 bits (43), Expect(2) = 9e-057
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +2 / +3
Query: 2 SPMKYHGLNVP 34
SPMKY+G+ P
Sbjct: 615 SPMKYYGMTEP 647
>gb|AF083332.1|AF083332 Medicago sativa cinnamyl-alcohol dehydrogenase (MsaCad2) mRNA,
complete cds
Length = 1324
Score = 150 bits (322), Expect = 3e-037
Identities = 64/141 (45%), Positives = 98/141 (69%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S KK+EAL+ LGAD ++VS D M+ AA ++D II+TV L PY+ LLK +G
Sbjct: 687 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 866
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K+I++G+ PL+ +++G K++ GS +G +++T+EM+ + G+T+ IE+V DY
Sbjct: 867 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 1046
Query: 482 VNTAMERLAKADVRYRFVIDI 544
+N A ERL K DVRYRFV+D+
Sbjct: 1047 INKAFERLEKNDVRYRFVVDV 1109
>emb|Z19573.1|MSCIALDHA M.sativa encoding cinnamyl alcohol dehydrogenase
Length = 1321
Score = 150 bits (322), Expect = 3e-037
Identities = 64/141 (45%), Positives = 98/141 (69%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S KK+EAL+ LGAD ++VS D M+ AA ++D II+TV L PY+ LLK +G
Sbjct: 711 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 890
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K+I++G+ PL+ +++G K++ GS +G +++T+EM+ + G+T+ IE+V DY
Sbjct: 891 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 1070
Query: 482 VNTAMERLAKADVRYRFVIDI 544
+N A ERL K DVRYRFV+D+
Sbjct: 1071 INKAFERLEKNDVRYRFVVDV 1133
>gb|L46857.1|ALFCAD1B Medicago sativa cinnamyl-alcohol dehydrogenase (cad1) mRNA, partial
cds
Length = 1201
Score = 150 bits (322), Expect = 3e-037
Identities = 64/141 (45%), Positives = 98/141 (69%)
Frame = +2 / +3
Query: 122 ISTSPGKKQEALDRLGADAFVVSKDAEEMKAAASTMDGIINTVSANLTLAPYMGLLKPNG 301
IS+S KK+EAL+ LGAD ++VS D M+ AA ++D II+TV L PY+ LLK +G
Sbjct: 564 ISSSDKKKKEALEDLGADNYLVSSDTVGMQEAADSLDYIIDTVPVGHPLEPYLSLLKIDG 743
Query: 302 KMIMVGLPTKPLEIPPFDLIIGNKTLAGSCIGGMRDTQEMINVAAKHGVTADIELVAADY 481
K+I++G+ PL+ +++G K++ GS +G +++T+EM+ + G+T+ IE+V DY
Sbjct: 744 KLILMGVINTPLQFVTPMVMLGRKSITGSFVGSVKETEEMLEFWKEKGLTSMIEIVTMDY 923
Query: 482 VNTAMERLAKADVRYRFVIDI 544
+N A ERL K DVRYRFV+D+
Sbjct: 924 INKAFERLEKNDVRYRFVVDV 986
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,302,342
Number of Sequences: 7669
Number of extensions: 101403
Number of successful extensions: 958
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 307798335
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)