TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3871943.2.1
(1085 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U28148.1|MSU28148 Medicago sativa putative pectinesteras... 154 7e-061
gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular tr... 215 6e-057
gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular tr... 111 2e-035
gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) ... 129 3e-032
gb|AY587267.1| Medicago sativa pectin methylesterase (PER) ... 129 4e-032
>gb|U28148.1|MSU28148 Medicago sativa putative pectinesterase mRNA, complete cds
Length = 1573
Score = 154 bits (331), Expect(2) = 7e-061
Identities = 62/147 (42%), Positives = 83/147 (56%)
Frame = -2 / +1
Query: 1084 PTQTVVTGDKSNXXXXXXXXXXXXXAEGNGFICKSMGFVNTAGPEGHQAVAMHVQGDKSV 905
PT+T TG + G F+ K +GF NTAG HQAVA+ V D+++
Sbjct: 643 PTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGKHQAVALRVTADQAI 822
Query: 904 FYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCLMTVRKPGDSQSNMV 725
FYNC+ +G+QDTLYV + RQF+RDC + GT+DF+FG +FQNC + R P Q +V
Sbjct: 823 FYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCKLVCRLPAKGQQCLV 1002
Query: 724 TAQGRTDPNMPTGIVLQGCRIVPEQAL 644
TA GR N + +V Q E AL
Sbjct: 1003 TAGGREKQNSASALVFQSSHFTGEPAL 1083
Score = 95.0 bits (201), Expect(2) = 7e-061
Identities = 37/82 (45%), Positives = 50/82 (60%)
Frame = -2 / +1
Query: 619 TYLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKRVN 440
+YLGRPWK+Y++ V+M+STI + PEG+ WMG +T Y EY N GPGA T+ RV
Sbjct: 1105 SYLGRPWKQYSKVVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVK 1284
Query: 439 WPGYHVIGQADATAFTAGAFID 374
W G V+ A + G F +
Sbjct: 1285 WHGVKVLTSNVAAEYYPGKFFE 1350
Score = 82.2 bits (173), Expect(2) = 2e-023
Identities = 50/116 (43%), Positives = 55/116 (47%)
Frame = +3 / -3
Query: 645 SACSGTMRQPWSTMPVGMLGSVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPS 824
SA S + W T + + RP AVT W G QFW + PK KS VP
Sbjct: 1082 SAGSPVK*ELWKTRALALFCFSRPPAVTRHCWPFAGNLQTSLQFWKTPKRSPKTKSIVPE 903
Query: 825 TSQSRKNCRLACTYSVSWYPSNRQL*NTDLSPCTCIATAWCPSGPAVFTNPIDLQM 992
QSR N R T SVSW PS QL*N S T ATA C PAVF+ PI M
Sbjct: 902 MLQSR*NWRWDWT*SVSWKPSI*QL*NIAWSAVTRRATA*CLPVPAVFSKPISFAM 735
Score = 41.4 bits (84), Expect(2) = 2e-023
Identities = 19/33 (57%), Positives = 20/33 (60%)
Frame = +3 / -3
Query: 519 PIHSAHPSGLMRSPMVLSITTVLAYSFHGRPR* 617
PIH PSG + MV SI T L Y FHG PR*
Sbjct: 1205 PIHGM*PSGTKIASMVESIITTLEYCFHGLPR* 1107
>gb|CO514932.1|CO514932 s13dSG63B1100093_397369 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 215 bits (465), Expect = 6e-057
Identities = 85/153 (55%), Positives = 102/153 (66%)
Frame = -2 / +2
Query: 949 GHQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNC 770
GHQAVA+ V D +V Y C GYQDT+Y H+NRQF+R+C++ GTVDFIFGN+A +FQNC
Sbjct: 2 GHQAVALRVGSDHAVVYRCNVIGYQDTMYAHSNRQFYRECDIYGTVDFIFGNAAVVFQNC 181
Query: 769 LMTVRKPGDSQSNMVTAQGRTDPNMPTGIVLQGCRIVPEQALFPDRLQIATYLGRPWKEY 590
+ RKP Q N +TAQ R DPN TGI + CRI+ Q L + TYLGRPWK Y
Sbjct: 182 SLYARKPMPYQKNTITAQNRKDPNQNTGISIHNCRILATQDLEASKGNFTTYLGRPWKLY 361
Query: 589 ARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYY 491
+RTV M S +GD I P GW EW L TLYY
Sbjct: 362 SRTVYMLSYMGDHIHPRGWLEWNTTFALDTLYY 460
Score = 119 bits (254), Expect = 7e-028
Identities = 64/142 (45%), Positives = 73/142 (51%)
Frame = +3 / -3
Query: 525 HSAHPSGLMRSPMVLSITTVLAYSFHGRPR*VAIWRRSGKSACSGTMRQPWSTMPVGMLG 704
HS HP G + SPM ++ TVL YSFHGRPR V + +C + Q W +PV G
Sbjct: 426 HSNHPRGCI*SPMYDNMYTVLEYSFHGRPRYVVKFPLEASRSCVARILQLWMDIPVF*FG 247
Query: 705 SVRPCAVTMLDWLSPGLRTVMRQFWNSAAELPKMKSTVPSTSQSRKNCRLACTYSVSWYP 884
S AV + W G QF + A LP MKSTVP S SR N R C Y VS YP
Sbjct: 246 SFLFWAVMVFFW*GMGFLA*RLQF*KTTAALPNMKSTVP*MSHSR*N*RFECAYIVS*YP 67
Query: 885 SNRQL*NTDLSPCTCIATAWCP 950
Q * T S T ATAWCP
Sbjct: 66 ITLQR*TTAWSDPTRRATAWCP 1
>gb|CO514488.1|CO514488 s13dSG43C1100084_327636 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 111 bits (236), Expect(3) = 2e-035
Identities = 40/72 (55%), Positives = 51/72 (70%)
Frame = -2 / +3
Query: 625 IATYLGRPWKEYARTVVMESTIGDLIRPEGWAEWMGDLGLKTLYYAEYANTGPGAGTSKR 446
+ ++LGRPW++Y+RTV M++ I I P GW W G+ L TLYYAEYANTG G+ TS R
Sbjct: 93 VKSHLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNR 272
Query: 445 VNWPGYHVIGQA 410
V W GYHV+ A
Sbjct: 273 VTWKGYHVLTSA 308
Score = 37.3 bits (75), Expect(3) = 2e-035
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -2 / +3
Query: 721 AQGRTDPNMPTGIVLQGCRIVPEQALFPDRLQ 626
AQGRTDPN TGI++ R+ + L P ++
Sbjct: 3 AQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVK 98
Score = 34.1 bits (68), Expect(3) = 2e-035
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = -2 / +3
Query: 412 ADATAFTAGAFIDGASWLQSTGTPNVMG 329
+ A+ FT G FI G SW+ +TG P G
Sbjct: 309 SQASPFTVGNFIAGNSWIGNTGVPFTSG 392
>gb|AY587268.1| Medicago sativa pectin methylesterase (PEF) mRNA, partial cds
Length = 351
Score = 129 bits (277), Expect(2) = 3e-032
Identities = 48/101 (47%), Positives = 67/101 (66%)
Frame = -2 / +1
Query: 946 HQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCL 767
HQAVA+ V D+++FYNC+ +G+QDTLYV + RQF+RDC + GT+DF+FG++ +FQNC
Sbjct: 4 HQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGDAFGVFQNCK 183
Query: 766 MTVRKPGDSQSNMVTAQGRTDPNMPTGIVLQGCRIVPEQAL 644
+ R P Q +VTA GR N + +V Q E AL
Sbjct: 184 LVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPAL 306
Score = 24.0 bits (46), Expect(2) = 3e-032
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = -2 / +1
Query: 619 TYLGRPWK 596
+YLGRPWK
Sbjct: 328 SYLGRPWK 351
>gb|AY587267.1| Medicago sativa pectin methylesterase (PER) mRNA, partial cds
Length = 351
Score = 129 bits (277), Expect(2) = 4e-032
Identities = 47/98 (47%), Positives = 66/98 (67%)
Frame = -2 / +1
Query: 946 HQAVAMHVQGDKSVFYNCRFEGYQDTLYVHANRQFFRDCEVLGTVDFIFGNSAALFQNCL 767
HQAVA+ V DK++FYNC+ +GYQ TL+ + RQF+RDC + GT+D ++G++ A+FQNC
Sbjct: 4 HQAVALRVTADKAIFYNCQIDGYQATLFAESQRQFYRDCSISGTIDMVYGDAFAVFQNCK 183
Query: 766 MTVRKPGDSQSNMVTAQGRTDPNMPTGIVLQGCRIVPE 653
+ VRKP + Q V A GRT + +G V Q C E
Sbjct: 184 LIVRKPLEEQQCFVAADGRTKSDSSSGFVFQSCHFTGE 297
Score = 23.5 bits (45), Expect(2) = 4e-032
Identities = 7/7 (100%), Positives = 7/7 (100%)
Frame = -2 / +1
Query: 616 YLGRPWK 596
YLGRPWK
Sbjct: 331 YLGRPWK 351
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,374,468
Number of Sequences: 7669
Number of extensions: 95905
Number of successful extensions: 861
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 292178718
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)