TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3866391.2.1
(613 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 65 2e-036
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 65 8e-036
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 61 1e-034
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 67 1e-034
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 66 1e-033
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 111 5e-033
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 114 2e-029
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 67 5e-026
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 65.2 bits (136), Expect(4) = 2e-036
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = +2 / +3
Query: 374 DCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGS 508
+C I GY IP G + N W + RDP W+R EF PERF+E G+
Sbjct: 1095 ECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETGA 1229
Score = 54.7 bits (113), Expect(4) = 2e-036
Identities = 20/53 (37%), Positives = 36/53 (67%)
Frame = +2 / +3
Query: 86 EYNLTRDQIKAQLVVMFEAGTDTSFIVLEYAMVQLMRNPHLMTKLQAEVRSTI 244
E +T++QIK +V +F AGTD++ + E+A+ +L+ NP ++ K + EV S +
Sbjct: 816 EIKITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEVYSVV 974
Score = 44.1 bits (90), Expect(4) = 2e-036
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2 / +3
Query: 530 KGNDFQYIPFGAGRRMCPAISFATA 604
+G FQ +PFG+GRRMCP ++ AT+
Sbjct: 1257 RGQHFQLLPFGSGRRMCPGVNLATS 1331
Score = 38.2 bits (77), Expect(4) = 2e-036
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = +2 / +3
Query: 263 IVTEDELHSLSYLKAVVKETLRLHMPAPLL 352
+V E + +L Y++A+VKET R+H P P++
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLPVV 1076
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 64.8 bits (135), Expect(4) = 8e-036
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = +2 / +3
Query: 374 DCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGS 508
+C I GY IP G + N W + RDP W+R EF PERF+E G+
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETGA 1229
Score = 52.8 bits (109), Expect(4) = 8e-036
Identities = 20/53 (37%), Positives = 34/53 (64%)
Frame = +2 / +3
Query: 86 EYNLTRDQIKAQLVVMFEAGTDTSFIVLEYAMVQLMRNPHLMTKLQAEVRSTI 244
E +T+D IK +V F AGTD++ + E+A+ +L+ NP ++ K + EV S +
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVV 974
Score = 44.1 bits (90), Expect(4) = 8e-036
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2 / +3
Query: 530 KGNDFQYIPFGAGRRMCPAISFATA 604
+G FQ +PFG+GRRMCP ++ AT+
Sbjct: 1257 RGQHFQLLPFGSGRRMCPGVNLATS 1331
Score = 38.2 bits (77), Expect(4) = 8e-036
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = +2 / +3
Query: 263 IVTEDELHSLSYLKAVVKETLRLHMPAPLL 352
+V E + +L Y++A+VKET R+H P P++
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLPVV 1076
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 61.1 bits (127), Expect(4) = 1e-034
Identities = 21/45 (46%), Positives = 27/45 (60%)
Frame = +2 / +3
Query: 374 DCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGS 508
+C I GY IP G + N W + RD W+R EF PERF+E G+
Sbjct: 1095 ECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETGA 1229
Score = 52.8 bits (109), Expect(4) = 1e-034
Identities = 20/53 (37%), Positives = 34/53 (64%)
Frame = +2 / +3
Query: 86 EYNLTRDQIKAQLVVMFEAGTDTSFIVLEYAMVQLMRNPHLMTKLQAEVRSTI 244
E +T+D IK +V F AGTD++ + E+A+ +L+ NP ++ K + EV S +
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEVYSVV 974
Score = 44.1 bits (90), Expect(4) = 1e-034
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2 / +3
Query: 530 KGNDFQYIPFGAGRRMCPAISFATA 604
+G FQ +PFG+GRRMCP ++ AT+
Sbjct: 1257 RGQHFQLLPFGSGRRMCPGVNLATS 1331
Score = 38.2 bits (77), Expect(4) = 1e-034
Identities = 13/30 (43%), Positives = 22/30 (73%)
Frame = +2 / +3
Query: 263 IVTEDELHSLSYLKAVVKETLRLHMPAPLL 352
+V E + +L Y++A+VKET R+H P P++
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLPVV 1076
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 67.0 bits (140), Expect(4) = 1e-034
Identities = 24/60 (40%), Positives = 35/60 (58%)
Frame = +2 / +2
Query: 350 LVPHLSMADCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGSAAAMDSN 529
L+ H + + T+ GY IP R ++N+WA+ RD +CWE E F P RF++ G SN
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLKPGVPDFKGSN 1405
Score = 46.0 bits (94), Expect(4) = 1e-034
Identities = 19/56 (33%), Positives = 33/56 (58%)
Frame = +2 / +2
Query: 77 LQQEYNLTRDQIKAQLVVMFEAGTDTSFIVLEYAMVQLMRNPHLMTKLQAEVRSTI 244
L LT+D IKA ++ + GT+T +E+AM +LM++P + K+Q E+ +
Sbjct: 962 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQELAEVV 1129
Score = 42.8 bits (87), Expect(4) = 1e-034
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +2 / +2
Query: 266 VTEDELHSLSYLKAVVKETLRLHMPAPLLV 355
V E + L+YLK +KETLRLH P PLL+
Sbjct: 1145 VEEPDFEKLTYLKCALKETLRLHPPIPLLL 1234
Score = 40.0 bits (81), Expect(4) = 1e-034
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +2 / +2
Query: 530 KGNDFQYIPFGAGRRMCPAISFATAA 607
KG++F++IPFG+GRR CP + A
Sbjct: 1394 KGSNFEFIPFGSGRRSCPGMQLGLYA 1471
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 65.7 bits (137), Expect(4) = 1e-033
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +2 / +1
Query: 350 LVPHLSMADCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGSAAAMDSN 529
L+ H + + T+ GY IP R ++N+WA+ RD +CW+ E F P RF++ G SN
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPGVPDFKGSN 1419
Score = 44.1 bits (90), Expect(4) = 1e-033
Identities = 18/56 (32%), Positives = 32/56 (57%)
Frame = +2 / +1
Query: 77 LQQEYNLTRDQIKAQLVVMFEAGTDTSFIVLEYAMVQLMRNPHLMTKLQAEVRSTI 244
L LT+D IKA ++ + GT+T +E+ M +LM++P + K+Q E+ +
Sbjct: 976 LHNSIKLTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQELAEVV 1143
Score = 42.8 bits (87), Expect(4) = 1e-033
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +2 / +1
Query: 266 VTEDELHSLSYLKAVVKETLRLHMPAPLLV 355
V E + L+YLK +KETLRLH P PLL+
Sbjct: 1159 VEEPDFEKLTYLKCALKETLRLHPPIPLLL 1248
Score = 40.0 bits (81), Expect(4) = 1e-033
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +2 / +1
Query: 530 KGNDFQYIPFGAGRRMCPAISFATAA 607
KG++F++IPFG+GRR CP + A
Sbjct: 1408 KGSNFEFIPFGSGRRSCPGMQLGLYA 1485
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 111 bits (236), Expect(2) = 5e-033
Identities = 43/88 (48%), Positives = 62/88 (70%)
Frame = +2 / +3
Query: 266 VTEDELHSLSYLKAVVKETLRLHMPAPLLVPHLSMADCTIQGYTIPSGTRTIVNSWALAR 445
VTE +LH L YL+AV+KETLRL M PLLVPH+++ D + G+ IP+ ++ +VN+W L
Sbjct: 1068 VTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDPKLNGFDIPAESKILVNAWWLPN 1247
Query: 446 DPSCWERAEEFMPERFMEGGSAAAMDSN 529
+P+ W++ EEF PERF+E S + N
Sbjct: 1248 NPAHWKKPEEFRPERFLEEESHVEANGN 1331
Score = 44.1 bits (90), Expect(2) = 5e-033
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = +2 / +3
Query: 533 GNDFQYIPFGAGRRMCPAISFA 598
GNDF+Y+PFG GRR CP I A
Sbjct: 1326 GNDFRYLPFGVGRRSCPGIILA 1391
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 114 bits (243), Expect(2) = 2e-029
Identities = 48/96 (50%), Positives = 61/96 (63%)
Frame = +2 / +2
Query: 209 MTKLQAEVRSTIINNGTEIVTEDELHSLSYLKAVVKETLRLHMPAPLLVPHLSMADCTIQ 388
M +A+V + + V E ELH L YLK+V+KET+RLH PLL+P S+ C I
Sbjct: 164 MVMEKAQVEVRRVFDKKGYVDETELHQLIYLKSVIKETMRLHPSLPLLIPRESIKPCQIN 343
Query: 389 GYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFM 496
GY IP+ TR IVN+WA+ RDP W AE F PERF+
Sbjct: 344 GYDIPAKTRVIVNAWAIGRDPRYWVDAESFKPERFL 451
Score = 28.6 bits (56), Expect(2) = 2e-029
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = +2 / +2
Query: 530 KGNDFQYIPFGA 565
KG DF+YIPFG+
Sbjct: 470 KGTDFEYIPFGS 505
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 66.6 bits (139), Expect(3) = 5e-026
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +2 / +2
Query: 350 LVPHLSMADCTIQGYTIPSGTRTIVNSWALARDPSCWERAEEFMPERFMEGGSAAAMDSN 529
L+ H + + T+ GY IP R ++N+WA+ RD +CW+ E F P RF++ G SN
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPGEPDFKGSN 265
Score = 42.8 bits (87), Expect(3) = 5e-026
Identities = 17/30 (56%), Positives = 21/30 (70%)
Frame = +2 / +2
Query: 266 VTEDELHSLSYLKAVVKETLRLHMPAPLLV 355
V E + L+YLK +KETLRLH P PLL+
Sbjct: 5 VEEPDFEKLTYLKCALKETLRLHPPIPLLL 94
Score = 40.0 bits (81), Expect(3) = 5e-026
Identities = 13/26 (50%), Positives = 19/26 (73%)
Frame = +2 / +2
Query: 530 KGNDFQYIPFGAGRRMCPAISFATAA 607
KG++F++IPFG+GRR CP + A
Sbjct: 254 KGSNFEFIPFGSGRRSCPGMQLGLYA 331
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,372,020
Number of Sequences: 7669
Number of extensions: 78903
Number of successful extensions: 1498
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 41
effective length of database: 934,139
effective search space used: 151330518
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)