TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3858636.2.1
         (907 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|X58180.1|MSPAL  M.sativa PAL mRNA for phenylalanine ammo...   249   2e-071
gb|CO516771.1|CO516771  s13dSG94C0200006_446870 Glandular tr...   100   2e-022

>emb|X58180.1|MSPAL M.sativa PAL mRNA for phenylalanine ammonia-lyase
          Length = 2409

 Score =  249 bits (539), Expect(3) = 2e-071
 Identities = 99/174 (56%), Positives = 132/174 (75%)
 Frame = +2 / +1

                                                                        
Query: 107  ARKVLTTSLGGDLHSARFSEKALLTAIDREAVYGYYDDPCSANSPLMKKIRAVLVDHAPP 286
            A+K LT  + G+LH +RF EK LL  +DRE V+ Y DDPCSA  PL +K+R VLVDHA  
Sbjct: 1753 AKKTLTMGVNGELHPSRFCEKDLLKVVDREHVFAYIDDPCSATYPLSQKLRQVLVDHALV 1932

                                                                        
Query: 287  SGEAEKDASASVFSKINRFEEELREALPREMEAARVAFETGAAPIANRIKESRSYPLYRF 466
            +GE+EK+ + S+F KI  FEEEL+  LP+E+E+AR A+E+G   I N+I   RSYPLY+F
Sbjct: 1933 NGESEKNFNTSIFQKIATFEEELKTLLPKEVESARTAYESGNPTIPNKINGCRSYPLYKF 2112

                                                                  
Query: 467  IRQDLGAVYLTGEKLKSPGEECNKVFLALSEGKLIDPMLDCLKEWDGKPLPIC* 628
            +R++LG   LTGE + SPGEEC+K+F A+ +GK+IDP+L+CL EW+G PLPIC*
Sbjct: 2113 VREELGTGLLTGENVISPGEECDKLFSAMCQGKIIDPLLECLGEWNGAPLPIC* 2274




 Score = 39.6 bits (80), Expect(3) = 2e-071
 Identities = 14/21 (66%), Positives = 17/21 (80%)
 Frame = +1 / +1

                                
Query: 7   ESDPLNWGKAAAELTGSHLEE 69
           E+DPLNWG AA  + GSHL+E
Sbjct: 190 EADPLNWGVAAEAMKGSHLDE 252




 Score = 21.7 bits (41), Expect(3) = 2e-071
 Identities = 7/11 (63%), Positives = 10/11 (90%)
 Frame = +1 / +1

                       
Query: 58   HLEENLKSAAR 90
            HLEENLK++ +
Sbjct: 1702 HLEENLKNSVK 1734



>gb|CO516771.1|CO516771 s13dSG94C0200006_446870 Glandular trichomes Medicago sativa cDNA,
           mRNA sequence
          Length = 404

 Score =  100 bits (213), Expect(2) = 2e-022
 Identities = 39/76 (51%), Positives = 55/76 (72%)
 Frame = +2 / +3

                                                                       
Query: 107 ARKVLTTSLGGDLHSARFSEKALLTAIDREAVYGYYDDPCSANSPLMKKIRAVLVDHAPP 286
           A+++LT  + G+LH +RF EK LL  ++ E V+ Y DDPCSA  PLM+K+R VLVDHA  
Sbjct: 174 AKRILTVGVNGELHPSRFCEKDLLNVVEGEYVFAYVDDPCSATYPLMQKLRHVLVDHALQ 353

                           
Query: 287 SGEAEKDASASVFSKI 334
           +G+ E ++S S+F KI
Sbjct: 354 NGDKEANSSTSIFQKI 401




 Score = 21.7 bits (41), Expect(2) = 2e-022
 Identities = 7/11 (63%), Positives = 9/11 (81%)
 Frame = +1 / +3

                      
Query: 58  HLEENLKSAAR 90
           H+EENLKS  +
Sbjct: 123 HIEENLKSVVK 155



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 5,331,085
Number of Sequences: 7669
Number of extensions: 68404
Number of successful extensions: 750
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 237050658
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)