TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748380.2.1
(1272 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516725.1|CO516725 s13dSG74F0300027_446778 Glandular tr... 146 1e-046
gb|CO515945.1|CO515945 s13dSG61B0400029_445218 Glandular tr... 147 1e-046
gb|AF335473.2|AF335473 Medicago sativa MADS box protein-lik... 106 9e-027
gb|L41727.1|ALFMBP Medicago sativa (clone nmh7) MADS-box pr... 103 2e-026
gb|AF042068.1|AF042068 Medicago sativa MADS-box protein NMH... 103 6e-023
gb|U91964.1|MSU91964 Medicago sativa MADS-box protein mRNA,... 102 1e-022
>gb|CO516725.1|CO516725 s13dSG74F0300027_446778 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 501
Score = 146 bits (314), Expect(2) = 1e-046
Identities = 61/81 (75%), Positives = 73/81 (90%)
Frame = -1 / +1
Query: 1203 MGRGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASD 1024
MGR KVQLKRIE+KINRQVTFSKRR GLLKKA EISVLCDAEVA+I+FS GKL+ Y++D
Sbjct: 91 MGRSKVQLKRIESKINRQVTFSKRRTGLLKKAKEISVLCDAEVALIIFSDNGKLFNYSTD 270
Query: 1023 SRMDKILERYERYSYAEKALI 961
S M++ILER+ERY YAE++L+
Sbjct: 271 SCMEQILERHERYGYAERSLL 333
Score = 96.4 bits (204), Expect(2) = 6e-026
Identities = 50/81 (61%), Positives = 52/81 (64%)
Frame = +2 / -2
Query: 974 SA*EYRS*RSRILSMRESEAYS*SLPLGEKTMTATSASQRTEISCAFFSSPFRRLEKVTC 1153
SA* Y S RS I SM ES S PL EK + ATSASQRT+IS AF S P EKVTC
Sbjct: 320 SA*PYLSWRSSICSMHESVE*LKSFPLSEKIIKATSASQRTDISLAFLSKPVLLFEKVTC 141
Query: 1154 RFILFSIRFSCTLPRPIFACL 1216
R IL SIRFSCTL PI L
Sbjct: 140 RLILLSIRFSCTLLLPIIVFL 78
Score = 55.1 bits (114), Expect(2) = 1e-046
Identities = 19/44 (43%), Positives = 33/44 (75%)
Frame = -1 / +1
Query: 966 LISAESESEGNWCHEYRKLKAKIETIQRCHKHLMGEDLESLNPK 835
L+ +++++ NW EY +LKAKIE ++R +H +GEDL+S++ K
Sbjct: 331 LVGNDTDTQENWTMEYTRLKAKIELLERNQRHYLGEDLDSMSHK 462
Score = 36.3 bits (73), Expect(2) = 6e-026
Identities = 18/36 (50%), Positives = 22/36 (61%)
Frame = +2 / -2
Query: 848 DSRSSPIRCLWHLCMVSILAFSFLYSWHQFPSLSDS 955
+S+SSP CLW L SI A S +YS QF +S S
Sbjct: 449 ESKSSPR*CLWFLSKSSIFALSLVYSIVQFS*VSVS 342
>gb|CO515945.1|CO515945 s13dSG61B0400029_445218 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 417
Score = 147 bits (316), Expect(2) = 1e-046
Identities = 61/81 (75%), Positives = 74/81 (91%)
Frame = -1 / +2
Query: 1203 MGRGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASD 1024
MGR KVQLKRIE+KINRQVTFSKRR GLLKKA EISVLCDAEVA+I+FS GKL++Y++D
Sbjct: 32 MGRSKVQLKRIESKINRQVTFSKRRTGLLKKAKEISVLCDAEVALIIFSDNGKLFDYSTD 211
Query: 1023 SRMDKILERYERYSYAEKALI 961
S M++ILER+ERY YAE++L+
Sbjct: 212 SCMEQILERHERYGYAERSLL 274
Score = 99.1 bits (210), Expect(2) = 6e-026
Identities = 51/81 (62%), Positives = 53/81 (65%)
Frame = +2 / -1
Query: 974 SA*EYRS*RSRILSMRESEAYS*SLPLGEKTMTATSASQRTEISCAFFSSPFRRLEKVTC 1153
SA* Y S RS I SM ES S S PL EK + ATSASQRT+IS AF S P EKVTC
Sbjct: 261 SA*PYLSWRSSICSMHESVE*SKSFPLSEKIIKATSASQRTDISLAFLSKPVLLFEKVTC 82
Query: 1154 RFILFSIRFSCTLPRPIFACL 1216
R IL SIRFSCTL PI L
Sbjct: 81 RLILLSIRFSCTLLLPIIVFL 19
Score = 54.2 bits (112), Expect(2) = 1e-046
Identities = 19/44 (43%), Positives = 32/44 (72%)
Frame = -1 / +2
Query: 966 LISAESESEGNWCHEYRKLKAKIETIQRCHKHLMGEDLESLNPK 835
L+ +++++ NW EY +LKAKIE ++R +H +GEDL S++ K
Sbjct: 272 LVGNDTDTQENWTMEYTRLKAKIELLERNQRHYLGEDLNSMSHK 403
Score = 33.6 bits (67), Expect(2) = 6e-026
Identities = 17/34 (50%), Positives = 20/34 (58%)
Frame = +2 / -1
Query: 854 RSSPIRCLWHLCMVSILAFSFLYSWHQFPSLSDS 955
+SSP CLW L SI A S +YS QF +S S
Sbjct: 384 KSSPR*CLWFLSKSSIFALSLVYSIVQFS*VSVS 283
>gb|AF335473.2|AF335473 Medicago sativa MADS box protein-like protein NGL9 mRNA, complete cds
Length = 801
Score = 106 bits (227), Expect(2) = 9e-027
Identities = 40/61 (65%), Positives = 55/61 (90%)
Frame = -1 / +1
Query: 1203 MGRGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASD 1024
MGRGK+++KRIEN NRQVT+SKR++G+LKKA+EI+VLCDA+V+ I+F+P GK++EY S
Sbjct: 1 MGRGKIEIKRIENSSNRQVTYSKRKSGILKKANEINVLCDAQVSTIIFAPSGKMHEYISP 180
Query: 1023 S 1021
S
Sbjct: 181 S 183
Score = 28.6 bits (56), Expect(2) = 9e-027
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = -1 / +1
Query: 936 NWCHEYRKLKAKIETIQRCHKHLMGEDLESLNPK 835
N +E KLK + E ++ +HL G+D+ +LN K
Sbjct: 256 NLRNEIEKLKKENEDMEIKLRHLKGKDINTLNYK 357
>gb|L41727.1|ALFMBP Medicago sativa (clone nmh7) MADS-box protein mRNA, complete cds
Length = 965
Score = 103 bits (220), Expect(2) = 2e-026
Identities = 42/65 (64%), Positives = 53/65 (81%)
Frame = -1 / +1
Query: 1203 MGRGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASD 1024
M RGK+Q+KRIEN NRQV +SKRRNGL KKA+E++VLCDA+V++I+FS GKL+EY S
Sbjct: 1 MARGKIQIKRIENTTNRQVIYSKRRNGLFKKANELTVLCDAKVSIIMFSSTGKLHEYISP 180
Query: 1023 SRMDK 1009
S K
Sbjct: 181 SASTK 195
Score = 30.9 bits (61), Expect(2) = 2e-026
Identities = 22/86 (25%), Positives = 37/86 (43%)
Frame = -1 / +1
Query: 936 NWCHEYRKLKAKIETIQRCHKHLMGEDLESLNPKXXXXXXXXXXXXLKHIRSRKSHLMAE 757
N +KLK +++ + MGE L L+ + LK IR RK ++
Sbjct: 256 NMQENLKKLKDVNRNLRKEIRQGMGECLNDLSMEELRLLEDEMDKALKAIRERKYKVITN 435
Query: 756 SISELQKKERSLQEENKALQKELSER 679
I +KK + +E + L ++L R
Sbjct: 436 QIDTQRKKFNNEREVDNRLLRDLDAR 513
>gb|AF042068.1|AF042068 Medicago sativa MADS-box protein NMH 7 (nmh 7) gene, complete cds
Length = 6359
Score = 103 bits (219), Expect = 6e-023
Identities = 41/61 (67%), Positives = 53/61 (86%)
Frame = -1 / +2
Query: 1203 MGRGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASD 1024
M RGK+Q+KRIEN NRQVT+SKRR+GL KKA+E++VLCDA+V++I+FS GKL+EY S
Sbjct: 1154 MARGKIQIKRIENTTNRQVTYSKRRDGLFKKANELTVLCDAKVSIIMFSSTGKLHEYISP 1333
Query: 1023 S 1021
S
Sbjct: 1334 S 1336
>gb|U91964.1|MSU91964 Medicago sativa MADS-box protein mRNA, partial cds
Length = 775
Score = 102 bits (217), Expect = 1e-022
Identities = 41/59 (69%), Positives = 50/59 (84%)
Frame = -1 / +1
Query: 1197 RGKVQLKRIENKINRQVTFSKRRNGLLKKAHEISVLCDAEVAVIVFSPKGKLYEYASDS 1021
RGK+Q+KRIEN NRQV FSKRRNGLLKKA E+++LCDAEV V++FS KLY++AS S
Sbjct: 1 RGKIQIKRIENTTNRQVIFSKRRNGLLKKAKELAILCDAEVGVMIFSSTAKLYDFASTS 177
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,270,207
Number of Sequences: 7669
Number of extensions: 96864
Number of successful extensions: 684
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 349144380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)