TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3696657.2.1
(1531 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY219848.1| Medicago sativa polygalactorunase PG11 precu... 192 9e-072
emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene 180 1e-058
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 197 5e-058
>gb|AY219848.1| Medicago sativa polygalactorunase PG11 precursor, gene, complete cds
Length = 2213
Score = 192 bits (414), Expect(4) = 9e-072
Identities = 78/167 (46%), Positives = 105/167 (62%)
Frame = -2 / +1
Query: 996 VINATVAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVGGSSKISILNST 817
V N+ V G+ +SK FH+ + + T VTAP D NTDG+H+G S+ I ILN+
Sbjct: 1228 VNNSMVHGLTSKDSKNFHVMVLGCNNFTFDGFTVTAPGDSPNTDGIHMGRSTDIKILNTN 1407
Query: 816 IGTGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNG 637
I TGDDC+S+G G + V ++ CGPG GISVG LG+Y E +V + V+NC L T NG
Sbjct: 1408 IATGDDCISLGDGSRKITVQNVKCGPGHGISVGSLGKYTTEDNVEGVIVKNCTLTATQNG 1587
Query: 636 VRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVCPGK 496
VRIK++ DA +T S + FE+I M V NP++IDQ +CP C K
Sbjct: 1588 VRIKTWPDAPGTITVSDIHFEDIIMNNVMNPVIIDQEYCPWNQCSKK 1728
Score = 117 bits (251), Expect(2) = 3e-028
Identities = 67/164 (40%), Positives = 82/164 (50%)
Frame = +2 / -1
Query: 506 HTFCGQKYWSMTMGLATSSILMFSKVRWEAVSTDSASTYDLMRTPLVVFLSTQFRTVMSL 685
H F GQ WSM G T +++ SK E V AS L+ TP V + QF T+
Sbjct: 1718 HWFHGQYSWSMMTGFMTLFMIISSKCISETVIVPGASGQVLILTPF*VAVRVQFLTITPS 1539
Query: 686 TSFSSL*RPRQPTLMPCPGPQVMLSTKTPLHPGPIETQSSPVPMVELRMLILEEPPTWTP 865
T S + PR PTL+P PGP T L P P E QSSPV M+ LR+LI + P W P
Sbjct: 1538 TLSSVVYFPRLPTLIP*PGPHFTFWTVIFLLPSPNEIQSSPVAMLVLRILISVDLPMWIP 1359
Query: 866 SVFSTSAGAVTFTSFTVMLSELSMLMWKNLELSSLIPATVALIT 997
SV S GAVT V L + WK L + P T+ L+T
Sbjct: 1358 SVLGLSPGAVTVKPSNVKLLHPKTITWKFLLSLEVRPWTMELLT 1227
Score = 59.7 bits (124), Expect(4) = 9e-072
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = -2 / +3
Query: 489 NSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGK 340
N S + + ++F+NI GTS TPE + L+CS PC GV L +V + + GK
Sbjct: 1845 NPSKIKLSKISFKNIQGTSGTPEGVVLICSSGVPCDGVELNNVGLTFNGK 1994
Score = 45.1 bits (92), Expect(4) = 9e-072
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -2 / +2
Query: 1317 KAVQDAWTSACEATGSATVVIPKGDYLVGPLNFTGPCK 1204
KA+ +AW AC +T +A ++IP G Y + + GPCK
Sbjct: 770 KAIGNAWKEACLSTTAAKIMIPGGTYRMNGVELKGPCK 883
Score = 38.2 bits (77), Expect(4) = 9e-072
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = -2 / +2
Query: 1146 KYTASWIELSHVNNIGITGSGTLDGQGTAVYSKSKTDNVKAMPNTLVLFHVIN 988
K WI+ ++ ++ ++G G DGQG VY K+ + N+ V ++N
Sbjct: 944 KAADQWIKFLYMEHLTLSGKGVFDGQGATVYKKAAPAAAWSGKNSNVKIFMVN 1102
Score = 27.6 bits (54), Expect(2) = 3e-028
Identities = 14/32 (43%), Positives = 15/32 (46%)
Frame = +2 / -2
Query: 362 TSMRETPLHGCVSEQSRLMASGVDDVPVMLRN 457
T TP HG Q R SGV +VP M N
Sbjct: 1972 TLFSSTPSHGTPLLQIRTTPSGVPEVP*MFLN 1877
>emb|Y11118.1|MSPOLGAL3 M.sativa PG3 gene
Length = 2185
Score = 180 bits (387), Expect(3) = 1e-058
Identities = 70/162 (43%), Positives = 101/162 (62%)
Frame = -2 / +1
Query: 990 NATVAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVGGSSKISILNSTIG 811
++ V I +SK FH+N+ + T V +TAP NTDG+H+G S+ + +LN+ I
Sbjct: 1213 HSIVRDITSKDSKNFHVNVLGCTNFTFDSVTITAPVTSINTDGIHIGRSTDVKVLNTNIA 1392
Query: 810 TGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNGVR 631
TGDDCVS+G G + V ++ CGPG GISVG LG+Y E+ V + V+NC + NT NGVR
Sbjct: 1393 TGDDCVSLGDGSRQITVQNVNCGPGHGISVGSLGKYPKEEAVEHVLVKNCTITNTDNGVR 1572
Query: 630 IKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVC 505
IK++ + + + FE+I M V NP++IDQ +CP C
Sbjct: 1573 IKTWPSSPGTSPITDMHFEDIIMVNVLNPVIIDQEYCPWNQC 1698
Score = 48.3 bits (99), Expect(3) = 1e-058
Identities = 18/47 (38%), Positives = 29/47 (61%)
Frame = -2 / +2
Query: 483 SHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAG 343
S + + V F+NI GTS T + + L+CS++ PC V L +V + + G
Sbjct: 1808 SKIKISKVIFKNIRGTSKTKDGVVLICSKSFPCDAVELNNVALTFNG 1948
Score = 41.8 bits (85), Expect(3) = 1e-058
Identities = 15/37 (40%), Positives = 25/37 (67%)
Frame = -2 / +3
Query: 1314 AVQDAWTSACEATGSATVVIPKGDYLVGPLNFTGPCK 1204
A +AW +AC +T ++ V+IP+G Y + ++ GPCK
Sbjct: 450 AFTNAWKAACGSTTASKVMIPRGVYKMHAVDVKGPCK 560
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 197 bits (424), Expect(2) = 5e-058
Identities = 80/175 (45%), Positives = 111/175 (63%)
Frame = -2 / +1
Query: 996 VINATVAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVGGSSKISILNST 817
V N+ V G+ +SK FH+ + ++IT +TAP D NTDG+H+G S+ + ILN+
Sbjct: 493 VNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTN 672
Query: 816 IGTGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNG 637
IGTGDDCVSIG G + V + CGPG G+SVG LG++ E++V ITV+NC L T NG
Sbjct: 673 IGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNG 852
Query: 636 VRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVCPGKRSNSSHVS 472
VRIK++ DA +T S + FE+I M V NP++IDQ + P C K + +S
Sbjct: 853 VRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKIKLS 1017
Score = 122 bits (262), Expect(2) = 2e-030
Identities = 69/164 (42%), Positives = 84/164 (51%)
Frame = +2 / -3
Query: 506 HTFCGQKYWSMTMGLATSSILMFSKVRWEAVSTDSASTYDLMRTPLVVFLSTQFRTVMSL 685
H F G WSM G T +++ SK E V AS L+ TPL V + QF TV+
Sbjct: 983 HWFHG*YSWSMMTGFLTFVMVISSKCISETVIVPGASGQVLILTPLSVAVRVQFLTVIPS 804
Query: 686 TSFSSL*RPRQPTLMPCPGPQVMLSTKTPLHPGPIETQSSPVPMVELRMLILEEPPTWTP 865
T S + PR PTL P PGPQ T L P PIETQSSPVPM+ LR+L + P W P
Sbjct: 803 TFSSVVNFPRLPTLRP*PGPQFTPWTVICLLPSPIETQSSPVPMLVLRILTSVDFPMWIP 624
Query: 866 SVFSTSAGAVTFTSFTVMLSELSMLMWKNLELSSLIPATVALIT 997
SV S GAV V+ + WK L + P T+ L+T
Sbjct: 623 SVLGLSPGAVIVKPSNVIFLHPKTITWKFLLSLEVTPRTMELLT 492
Score = 47.8 bits (98), Expect(2) = 5e-058
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = -2 / +1
Query: 1329 TDCTKAVQDAWTSACEATGSATVVIPKGDYLVGPLNFTGPCK 1204
+D +A+ AW AC +T +A +VIP G Y + + GPCK
Sbjct: 136 SDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCK 261
Score = 25.4 bits (49), Expect(2) = 2e-030
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = +2 / -3
Query: 362 TSMRETPLHGCVSEQSRLMASGVDDVPVMLRN 457
T TP HG Q R S V +VP+ N
Sbjct: 1121 TLFSSTPSHGTALLQIRTTPSAVPEVPLTFLN 1026
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,598,790
Number of Sequences: 7669
Number of extensions: 131045
Number of successful extensions: 933
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 423676380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)