TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3671909.2.1
(767 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 92 2e-026
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 92 2e-026
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 92 2e-026
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 91.8 bits (194), Expect(2) = 2e-026
Identities = 35/65 (53%), Positives = 47/65 (72%)
Frame = -1 / +1
Query: 581 EFKGADFELLPFGAGRRMCPGMAFGLANVELALASLLFHFDWEAPGVSDPAEFDMAEAFG 402
+FKG++FE +PFG+GRR CPGM GL ++LA+A LL F WE P P+E DM++ FG
Sbjct: 1402 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1581
Query: 401 ITARR 387
+TA R
Sbjct: 1582 LTAPR 1596
Score = 41.4 bits (84), Expect(2) = 2e-026
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -1 / +1
Query: 749 LLPRQSQEPRRVLGYDVPAGTTVLVNVWALGRDGRYW 639
LL ++ E V GY +P V++N WA+GRD W
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 1350
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 91.8 bits (194), Expect(2) = 2e-026
Identities = 35/65 (53%), Positives = 47/65 (72%)
Frame = -1 / +2
Query: 581 EFKGADFELLPFGAGRRMCPGMAFGLANVELALASLLFHFDWEAPGVSDPAEFDMAEAFG 402
+FKG++FE +PFG+GRR CPGM GL ++LA+A LL F WE P P+E DM++ FG
Sbjct: 1388 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 1567
Query: 401 ITARR 387
+TA R
Sbjct: 1568 LTAPR 1582
Score = 41.4 bits (84), Expect(2) = 2e-026
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -1 / +2
Query: 749 LLPRQSQEPRRVLGYDVPAGTTVLVNVWALGRDGRYW 639
LL ++ E V GY +P V++N WA+GRD W
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 1336
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 91.8 bits (194), Expect(2) = 2e-026
Identities = 35/65 (53%), Positives = 47/65 (72%)
Frame = -1 / +2
Query: 581 EFKGADFELLPFGAGRRMCPGMAFGLANVELALASLLFHFDWEAPGVSDPAEFDMAEAFG 402
+FKG++FE +PFG+GRR CPGM GL ++LA+A LL F WE P P+E DM++ FG
Sbjct: 248 DFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMSDVFG 427
Query: 401 ITARR 387
+TA R
Sbjct: 428 LTAPR 442
Score = 41.4 bits (84), Expect(2) = 2e-026
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = -1 / +2
Query: 749 LLPRQSQEPRRVLGYDVPAGTTVLVNVWALGRDGRYW 639
LL ++ E V GY +P V++N WA+GRD W
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCW 196
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,914,189
Number of Sequences: 7669
Number of extensions: 54611
Number of successful extensions: 561
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 197338110
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)