TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665451.2.1
(911 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512932.1|CO512932 s13dSG86E0600039_121790 Glandular tr... 67 5e-028
gb|CO515733.1|CO515733 s13dSG77B1200101_419615 Glandular tr... 73 5e-028
gb|CO513560.1|CO513560 s13dSG12H0800064_139902 Glandular tr... 70 9e-025
gb|CO512672.1|CO512672 s13dSG19C0200006_121270 Glandular tr... 62 1e-024
gb|AJ410143.1|AJ410143 AJ410143 Medicago sativa library (Ka... 70 4e-022
gb|CO516142.1|CO516142 s13dSG65H0600060_445612 Glandular tr... 73 3e-021
>gb|CO512932.1|CO512932 s13dSG86E0600039_121790 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 569
Score = 66.6 bits (139), Expect(3) = 5e-028
Identities = 26/52 (50%), Positives = 35/52 (67%)
Frame = +3 / +2
Query: 207 RAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRLTKPFFMAI 362
+ I+ES +I EY+DE + PLLP PY RA ARFWADF E +L ++A+
Sbjct: 209 KPIAESFIILEYIDETWKQSPLLPQHPYERALARFWADFAEQKLLNAAWIAM 364
Score = 48.7 bits (100), Expect(3) = 5e-028
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +3 / +2
Query: 30 VKLIGFFGSPYAFRAEAALCLKGVPYELILEDLFGSKSELL 152
VKL+GF+ SP+ R E AL LKGV YE + ED+F LL
Sbjct: 38 VKLVGFWVSPFVKRVEWALKLKGVDYEYVEEDIFNKTPLLL 160
Score = 41.4 bits (84), Expect(3) = 5e-028
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +3 / +2
Query: 444 GKRFFAGDRPGYLDVAASALGPWRSVIEELNGVALL 551
GK+FF GD GYLD+A + W V EE+ + ++
Sbjct: 443 GKKFFGGDNIGYLDLALGWISYWLPVFEEVGSMQII 550
>gb|CO515733.1|CO515733 s13dSG77B1200101_419615 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 72.5 bits (152), Expect(3) = 5e-028
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +3 / +2
Query: 201 DGRAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRLTKPF 350
DG++ISESLVI EYVDE + PLLP DPY RA ARFWA F + + F
Sbjct: 215 DGKSISESLVILEYVDEIWKQNPLLPHDPYERAKARFWAKFADDKCVPAF 364
Score = 42.3 bits (86), Expect(3) = 5e-028
Identities = 17/42 (40%), Positives = 26/42 (61%)
Frame = +3 / +2
Query: 36 LIGFFGSPYAFRAEAALCLKGVPYELILEDLFGSKSELLLHH 161
L+GF+GSP+ R + AL LKG+ Y+ + ED LL ++
Sbjct: 56 LLGFWGSPFVLRVKWALELKGIQYQYVEEDFRNKSDMLLQYN 181
Score = 41.8 bits (85), Expect(3) = 5e-028
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +3 / +2
Query: 444 GKRFFAGDRPGYLDVAASALGPWRSVIEELNGVALL 551
G+R+F G+ G+ D+A G W VIEE G+ L+
Sbjct: 455 GRRYFGGENIGFADIAVGIFGFWARVIEETVGINLI 562
>gb|CO513560.1|CO513560 s13dSG12H0800064_139902 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 585
Score = 70.3 bits (147), Expect(3) = 9e-025
Identities = 27/49 (55%), Positives = 36/49 (73%)
Frame = +3 / +3
Query: 201 DGRAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRLTKP 347
+G+ ISESLVI EY+DE + P+LP+DPY RA ARFW+ FI+ + P
Sbjct: 231 NGKPISESLVILEYIDETWKQNPILPSDPYKRALARFWSKFIDDKCMIP 377
Score = 39.1 bits (79), Expect(3) = 9e-025
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = +3 / +3
Query: 15 SSPPPVKLIGFFGSPYAFRAEAALCLKGVPYE 110
S+ VKL G GSP++ R E AL LKGV Y+
Sbjct: 51 SNQEEVKLFGLVGSPFSTRVEIALKLKGVEYK 146
Score = 36.3 bits (73), Expect(3) = 9e-025
Identities = 13/26 (50%), Positives = 21/26 (80%)
Frame = +3 / +3
Query: 120 EDLFGSKSELLLHHNPVHKKVPVLLH 197
E+ G+ S+ ++ +NPV+KKVPVL+H
Sbjct: 153 EEKQGNLSDTVIKYNPVYKKVPVLVH 230
>gb|CO512672.1|CO512672 s13dSG19C0200006_121270 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 62.5 bits (130), Expect(3) = 1e-024
Identities = 24/44 (54%), Positives = 30/44 (68%)
Frame = +3 / +2
Query: 207 RAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRL 338
+ I+E +I EY+DE + PLLP PY RA ARFWADF E +L
Sbjct: 263 KPIAELFIILEYIDETWKQSPLLPQHPYERALARFWADFAEQKL 394
Score = 48.7 bits (100), Expect(3) = 1e-024
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = +3 / +2
Query: 30 VKLIGFFGSPYAFRAEAALCLKGVPYELILEDLFGSKSELL 152
VKL+GF+ SP+ R E AL LKGV YE + ED+F LL
Sbjct: 92 VKLVGFWVSPFVKRVEWALKLKGVDYEYVEEDIFNKTPLLL 214
Score = 34.1 bits (68), Expect(3) = 1e-024
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3 / +2
Query: 444 GKRFFAGDRPGYLDVAASALGPW 512
GK+FF GD GYLD+A + W
Sbjct: 497 GKKFFGGDNIGYLDLALGWISYW 565
>gb|AJ410143.1|AJ410143 AJ410143 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U62, mRNA sequence
Length = 822
Score = 70.3 bits (147), Expect(3) = 4e-022
Identities = 27/49 (55%), Positives = 36/49 (73%)
Frame = +3 / +1
Query: 201 DGRAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRLTKP 347
+G+ ISESLVI EY+DE + P+LP+DPY RA ARFW+ FI+ + P
Sbjct: 151 NGKPISESLVILEYIDETWKQNPILPSDPYKRALARFWSKFIDDKCMIP 297
Score = 36.3 bits (73), Expect(3) = 4e-022
Identities = 13/26 (50%), Positives = 21/26 (80%)
Frame = +3 / +1
Query: 120 EDLFGSKSELLLHHNPVHKKVPVLLH 197
E+ G+ S+ ++ +NPV+KKVPVL+H
Sbjct: 73 EEKQGNLSDTVIKYNPVYKKVPVLVH 150
Score = 29.9 bits (59), Expect(3) = 4e-022
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +3 / +1
Query: 51 GSPYAFRAEAALCLKGVPYE 110
GSP++ R E AL LKGV Y+
Sbjct: 7 GSPFSTRVEIALKLKGVEYK 66
>gb|CO516142.1|CO516142 s13dSG65H0600060_445612 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 368
Score = 72.5 bits (152), Expect(2) = 3e-021
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = +3 / +1
Query: 201 DGRAISESLVIAEYVDEAFDGPPLLPADPYARAAARFWADFIETRLTKPF 350
DG++ISESLVI EYVDE + PLLP DPY RA ARFWA F + + F
Sbjct: 217 DGKSISESLVILEYVDEIWKQNPLLPHDPYERAKARFWAKFADDKCVPAF 366
Score = 43.7 bits (89), Expect(2) = 3e-021
Identities = 16/27 (59%), Positives = 24/27 (88%)
Frame = +3 / +1
Query: 117 LEDLFGSKSELLLHHNPVHKKVPVLLH 197
+E+ F +KS++LL +NPV+KKVPVL+H
Sbjct: 136 VEEDFRNKSDMLLQYNPVYKKVPVLVH 216
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,703,288
Number of Sequences: 7669
Number of extensions: 56330
Number of successful extensions: 2127
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 238888260
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)