TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3570920.2.1
(786 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516658.1|CO516658 s13dSG66G1100086_446644 Glandular tr... 110 2e-035
gb|AY804253.1| Medicago sativa acidic glucanase (Glu) mRNA,... 88 6e-032
gb|CB165091.2|CB165091 15III Alfalfa developing flower bud ... 126 5e-030
gb|U27179.1|MSU27179 Medicago sativa acidic glucanase mRNA,... 88 3e-026
>gb|CO516658.1|CO516658 s13dSG66G1100086_446644 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 533
Score = 110 bits (237), Expect(4) = 2e-035
Identities = 41/96 (42%), Positives = 66/96 (68%)
Frame = +1 / +2
Query: 340 ESQDVIDPNTGLVYTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKGAVKETGATPD 519
++ V+D L Y N+FDAQ+D++ A+ AL + +KI ++E+GWP+ G E GA D
Sbjct: 245 QNPGVVDSGNHLKYDNLFDAQIDAVNAAMSALQYDDVKITVSETGWPSLGDSNEIGAGQD 424
Query: 520 NAQTYNTNLIRHVVNDSGTPAKPGEEIDVYIFSLFN 627
N +YN NL++ V+N SGTP +P + ++V++F+LFN
Sbjct: 425 NTASYNGNLVKRVLNGSGTPLRPKDPLNVFLFALFN 532
Score = 35.3 bits (71), Expect(4) = 2e-035
Identities = 16/35 (45%), Positives = 22/35 (62%)
Frame = +1 / +3
Query: 97 ALKKAGLHKKITISSTHSLGILSRSFPPSAGAFNS 201
AL+K L K+I ISS +L L S+P S G+F +
Sbjct: 3 ALQKQNLDKQILISSPIALSALQSSYPTSTGSFKT 107
Score = 28.4 bits (56), Expect(4) = 2e-035
Identities = 10/22 (45%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 217 LKPMLQFLVENQAPFMVDLYPY 282
+KPML+FL + + MV+ YP+
Sbjct: 126 IKPMLEFLRQTGSYLMVNAYPF 191
Score = 24.3 bits (47), Expect(4) = 2e-035
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +1 / +2
Query: 280 YYAYQNSRSNVSLNYALF 333
++AY + +SL+YALF
Sbjct: 188 FFAYAANSDTISLDYALF 241
>gb|AY804253.1| Medicago sativa acidic glucanase (Glu) mRNA, complete cds
Length = 1113
Score = 88.2 bits (187), Expect(4) = 6e-032
Identities = 35/102 (34%), Positives = 62/102 (60%)
Frame = +1 / +1
Query: 67 VVPAMRNVHTALKKAGLHKKITISSTHSLGILSRSFPPSAGAFNSSYAYFLKPMLQFLVE 246
V+PA +N++ A++ LH +I +S+ + ++ SFPPS G+F + +L P + +LV
Sbjct: 424 VLPATQNIYQAIRAKNLHDQIKVSTAIDMTLIGNSFPPSKGSFRNDVRAYLDPFIGYLVY 603
Query: 247 NQAPFMVDLYPYYAYQNSRSNVSLNYALFSPESQDVIDPNTG 372
AP +V++YPY+++ + ++SL YALF+ V D G
Sbjct: 604 AGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMVQDGPNG 729
Score = 42.6 bits (87), Expect(4) = 6e-032
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +1 / +1
Query: 379 YTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKG 489
Y N+FDA +DS+ AL + ++++ESGWP+ G
Sbjct: 730 YQNLFDAMLDSVHAALDNTGIGWVNVVVSESGWPSDG 840
Score = 30.7 bits (61), Expect(4) = 6e-032
Identities = 10/32 (31%), Positives = 20/32 (62%)
Frame = +1 / +1
Query: 649 ESERNWGLFFPDKSSIYSLDWTGRGNVDVMTG 744
E E+++G+F+P+K Y + G N +++ G
Sbjct: 979 ELEKHFGVFYPNKQKKYPFGFGGERNGEIVNG 1074
Score = 25.7 bits (50), Expect(4) = 6e-032
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +1 / +1
Query: 517 DNAQTYNTNLIRHV 558
DNA+ Y NLIRHV
Sbjct: 859 DNARIYLDNLIRHV 900
>gb|CB165091.2|CB165091 15III Alfalfa developing flower bud library Medicago sativa cDNA,
mRNA sequence
Length = 289
Score = 126 bits (270), Expect = 5e-030
Identities = 54/62 (87%), Positives = 57/62 (91%)
Frame = +1 / +3
Query: 10 TYITVGAEVTESPANVSALVVPAMRNVHTALKKAGLHKKITISSTHSLGILSRSFPPSAG 189
TYITVGAEVTESP N+SALVVPAM NV ALKKAGLHKKI +SSTHSLG+LSRSFPPSAG
Sbjct: 102 TYITVGAEVTESPENISALVVPAMTNVLAALKKAGLHKKIKVSSTHSLGVLSRSFPPSAG 281
Query: 190 AF 195
AF
Sbjct: 282 AF 287
>gb|U27179.1|MSU27179 Medicago sativa acidic glucanase mRNA, complete cds
Length = 1374
Score = 88.2 bits (187), Expect(3) = 3e-026
Identities = 35/102 (34%), Positives = 62/102 (60%)
Frame = +1 / +1
Query: 67 VVPAMRNVHTALKKAGLHKKITISSTHSLGILSRSFPPSAGAFNSSYAYFLKPMLQFLVE 246
V+PA +N++ A++ LH +I +S+ + ++ SFPPS G+F + +L P + +LV
Sbjct: 475 VLPATQNIYQAIRAKNLHDQILVSTAIDMTLIGNSFPPSKGSFRNDVRAYLDPFIGYLVY 654
Query: 247 NQAPFMVDLYPYYAYQNSRSNVSLNYALFSPESQDVIDPNTG 372
AP +V++YPY+++ + ++SL YALF+ V D G
Sbjct: 655 AGAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMVQDGPNG 780
Score = 42.6 bits (87), Expect(3) = 3e-026
Identities = 15/37 (40%), Positives = 24/37 (64%)
Frame = +1 / +1
Query: 379 YTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKG 489
Y N+FDA +DS+ AL + ++++ESGWP+ G
Sbjct: 781 YQNLFDAMLDSVHAALDNTGIGWVNVVVSESGWPSDG 891
Score = 24.8 bits (48), Expect(3) = 3e-026
Identities = 8/23 (34%), Positives = 15/23 (65%)
Frame = +1 / +1
Query: 649 ESERNWGLFFPDKSSIYSLDWTG 717
E E+++G+F+P+K Y + G
Sbjct: 1027 ELEKHFGVFYPNKQKKYPFGFGG 1095
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.316 0.132 0.390
Matrix: BLOSUM62
Number of Hits to DB: 6,634,728
Number of Sequences: 7669
Number of extensions: 89942
Number of successful extensions: 1177
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 200298618
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)